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Unit 8 test
Biotech study guide
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Test format
• 20 questions– Know vocabulary– Be able to place the steps for recombinant DNA
technology
• 1 open-ended (10 points)– Be able to read a DNA fingerprint– Explain the process of gel electrophoresis– How this technology is used to solve crimes
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What kind of cells are used to make animal clones?
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What kind of cells are used to make animal clones?
Body cells and egg cells
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This is a unique gel electrophoresis banding pattern produced by a person’s restriction fragments
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This is a unique gel electrophoresis banding pattern produced by a person’s restriction fragments
DNA fingerprint
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Similar to DNA replication because it makes lots of copies of the DNA
molecule
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Similar to DNA replication because it makes lots of copies of the DNA
molecule
PCR
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Small circular DNA molecules that are naturally found in bacteria
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Small circular DNA molecules that are naturally found in bacteria
plasmids
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Knowing the sequence of an organism’s DNA allows scientists to
study…
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Knowing the sequence of an organism’s DNA allows scientists to
study…
specific genes
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This is combining genes from different sources into a single DNA
molecule
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This is combining genes from different sources into a single DNA
molecule
recombinant DNA
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What process separates DNA fragments by their size and charge
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What process separates DNA fragments by their size and charge
gel electrophoresis
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During genetic modification, the organism’s cells take in ________
from another ___________.
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During genetic modification, the organism’s cells take in
__DNA____ from another __source____.
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What was added to the blood samples to create these fragments?
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What was added to the blood samples to create these fragments?
Restriction enzymes
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What is this process?
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What is this process?
Gel electrophoresis – be able to explain this process
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Who committed the crime?
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Who committed the crime?Suspect 2
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What would be possible sources for these DNA samples?
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What would be possible sources for these DNA samples?
Blood, hair, skin cells, bodily fluids
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What is the correct order?____ Insert recombinant DNA into bacteria cells
____ Isolate desired product from bacteria cells
____ Cut plasmid and desired gene with the same restriction enyzmes
____ Clone bacteria cells containing recombinant DNA
____ Combine plasmid and gene fragment, creating recombinant DNA
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What is the correct order?__3_ Insert recombinant DNA into bacteria cells
__5_ Isolate desired product from bacteria cells
__1_ Cut plasmid and desired gene with the same restriction enyzmes
__4_ Clone bacteria cells containing recombinant DNA
__2_ Combine plasmid and gene fragment, creating recombinant DNA
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What was used to cut this piece of DNA?
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What was used to cut this piece of DNA?
Restriction enzymes
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What are C & D?sticky ends
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If a scientists wants to make a human protein (like insulin) using
bacteria cells – what would be their first step?
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If a scientists wants to make a human protein (like insulin) using
bacteria cells – what would be their first step?
Use a restriction enzyme to cut out the human gene and the plasmid
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A stretch of DNA that varies among individuals is called a…
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A stretch of DNA that varies among individuals is called a…
genetic marker
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In this gel sample, band 3 moved further than all the other bands.
Why?
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In this gel sample, band 3 moved further
than all the other bands. Why?
Moved faster because it is smaller than other
pieces
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What are some advantages to transgenic (GMO) plants?
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What are some advantages to transgenic (GMO) plants?
More nutrientsresist pests
resist herbicideslonger shelf-life