(updated June 3, 2019) CURRICULUM VITAE GEORGE A. GARCIA ... · CURRICULUM VITAE GEORGE A. GARCIA, Ph. D. Professor and Chair of Medicinal Chemistry ... Committee on Institutional

(updated June 3, 2019)

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CURRICULUM VITAE GEORGE A. GARCIA, Ph. D.

Professor and Chair of Medicinal Chemistry Director, Interdepartmental Program in Medicinal Chemistry

BUSINESS ADDRESS HOME ADDRESS

The University of Michigan 8465 Parkridge Dr. College of Pharmacy Dexter, Michigan 48130 428 Church Street phone: (734) 330-3890 Ann Arbor, Michigan 48109-1065 Phone: (734) 764-2202 Fax: (734) 647-8430 E-mail: [email protected] URL: http://pharmacy.umich.edu/garcia-lab

EDUCATION AND ADVANCED TRAINING American Association of Colleges of Pharmacy, Academic Leadership Fellow, 2014-2015 American Association of Colleges of Pharmacy, Leadership Seminar, July 13, 2013 Committee on Institutional Cooperation, Academic Leadership Program Fellow, 2011-2012 Academic Impressions Workshop on “Evaluating Faculty to Improve Classroom Performance”, Vail, Colorado, June 7-9, 2006 Postdoctoral Fellow (Advisor: Sir Alan R. Fersht) Department of Chemistry, Cambridge University, England, 1988-1990 Ph. D., Pharmaceutical Chemistry (Advisor: George L. Kenyon) University of California, San Francisco, 1983-1988 B. S., Chemistry California State University, Sacramento, January 1982

PROFESSIONAL APPOINTMENTS 2012-present: Professor and Chair, Department of Medicinal Chemistry Director, Interdepartmental Program in Medicinal Chemistry University of Michigan, College of Pharmacy 2016-2017: Co-Director, Pharmacological Sciences Training Program 2015-2016: Director, Pharmacological Sciences Training Program 2011-2012: Associate-Chair, Department of Medicinal Chemistry University of Michigan, College of Pharmacy 2002-2012: Associate Professor of Medicinal Chemistry University of Michigan, College of Pharmacy 1996-2002: Associate Professor of Medicinal Chemistry and Pharmacognosy University of Michigan, College of Pharmacy 1990-1996: Assistant Professor of Medicinal Chemistry and Pharmacognosy University of Michigan, College of Pharmacy


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AWARDS AND HONORS 2005: University of Michigan, College of Pharmacy Student Appreciation Award 1988-1990: National Institutes of Health Postdoctoral Research Fellowship 1987: American Foundation for Pharmaceutical Education, "1987 Johnson & Johnson Pharmaceutical Chemistry Fellow" 1985-1987: American Foundation for Pharmaceutical Education, "H. A. B. Dunning Memorial Fellow" 1983-1986: University of California Regent's Fellowship 1984-1985: University of California, San Francisco Graduate Division, Minority Mentorship

PROFESSIONAL SERVICE Reviewing

Grant Proposals: • Ad-Hoc NIH –SBC-B Study Section, February 2017. • Ad-Hoc NIH –COBRE Study Section, June 2016. • Ad-Hoc M. J. Murdock Charitable Trust, November 2015. • Ad-Hoc Israel Science Foundation, August 2015. • Ad-Hoc NIH –SBIR Study Section, June 2015. • Ad-Hoc NIH – NIAID R21/R33 Review Panel Chair, Nov. 2013. • Ad-Hoc Kuwait University, 2007, 2008, 2011, 2012, 2013, 2015, 2017. • Ad-Hoc NSF – CHE-Organic Synthesis: Bioorganic and Macromolecular Materials March

2007. • Ad-Hoc NIH – INRSA Study Section, March 2006, June 2007. • Ad-Hoc NIH – Special Emphasis Panel, June 2005. • Ad-Hoc Science Foundation Ireland, Site Visit, April 2008, Mail-In Reviewer, January & June

2005. • Ad-Hoc NIH - Molecular & Cellular Biophysics Study Section, Telephone Review, July-2003. • Ad-Hoc Chairmen of European Research Councils’ Chemistry Committees (CERC3), Mail-In

Reviewer, April 2003. • Ad-Hoc NASA-Exobiology Program, Mail-In Reviewer, December 2002. • NIH - Bioorganic and Natural Products Chemistry Study Section, Oct 1999-2003. • Ad-Hoc Austrian Science Fund (FWF), May 2001. • Ad-Hoc NSF - Molecular Biochemistry Program, 1997, 1998, 1999, 2001. • Ad-Hoc NIH - Bioorganic and Natural Products Chemistry Study Section, Oct 17-18, 1996. • Ad-Hoc North Carolina Biotechnology Center, Jan. 1995 • Ad-Hoc NIH Site Visit, Universidad Central del Caribe, San Juan, Puerto Rico, Sept. 22-24,

1993 Paper Manuscripts:

• ACS Infectious Diseases • Archiv der Pharmazie • Biochimica et Biophysica Acta • Biochemistry • Bioorganic Chemistry • Biochimie • BioTechniques • Bioorganic and Med Chem Letters


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• Bioorganic and Medicinal Chemistry • BMC Infectious Diseases • Chemical Biology and Drug Design • Expert Opinion Drug Met & Tox • Inorganic Chemistry • Journal of Biological Chemistry • Journal of Medicinal Chemistry • Journal of Molecular Biology • Journal of Organic Chemistry • Nature: Scientific Reports • PNAS • RNA • Science

Promotion/Tenure for Other Universities: • Professor of Pharmaceutical Science, Professor of Medicinal Chemistry (2), 2017 • Professor of Medicinal Chemistry, 2015 (2) • Professor of Medicinal Chemistry, 2014 • Professor of Biochemistry and Molecular Biology, 2014 • Professor of Medicinal Chemistry, 2013 • Associate Professor of Medicinal Chemistry, 2012 • Associate Professor of Chemistry, 2005 • Professor of Chemistry, 1999

Academic Program Reviewer: • University of Toledo, Department of Medicinal Chemistry and Biological Chemistry (2018)

Editorial Board Member: • Molecular Biotechnology (1999-2001)

Cofounder and Coordinator (1990-1992) University of Michigan, Enzyme Discussion Group

Organizing Committee Chair (2002) Sixth Annual Meeting of the Michigan RNA Society

Program Chair (2011) 31st Midwest Enzyme Chemistry Conference Memberships: • American Association of Colleges of Pharmacy • American Chemical Society, Divisions of Medicinal Chemistry and Biological Chemistry • American Society for Biochemistry and Molecular Biology • International Society for Nucleosides, Nucleotides and Nucleic Acids • The RNA Society • Rho Chi • Sigma Xi

UNIVERSITY SERVICE University-wide:

1995-present: Chemistry-Biology Interface Training Program Faculty

1990-present: Pharmacological Sciences Training Program Faculty

1997-2018: Pharmacological Sciences Training Program, Executive Committee Member

2013-2015: Elected College of Pharmacy Representative to the University Faculty Senate Assembly

2004-2010: Chemical Biology Interdepartmental Program, Program Committee Member

2002-2004: Center for Research on Learning and Teaching, Advisory Board Member

2000-2002: Rackham Graduate School, Divisional Board Member


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1998 & 2003: Fulbright Campus Committee, Applicant Interviewer

1997-2006: Cellular and Molecular Biology Training Program Faculty

1995-1998: Biomedical Research Council, College of Pharmacy Representative

1995-1998: Rackham Graduate School, Merit Fellowship Selection Committee College of Pharmacy:

2018-present: Connections Committee

2016-present: Strategic Planning Committee

2015-present: Graduate Education Committee 2012-present: Administrative Operations Committee (ex officio, formerly Policy Review)

2012-present: Executive Committee (ex officio) (2007-2010, 1998-2001, elected faculty member)

2015: Chair Ad-Hoc EC Bylaws Review Subcommittee

2014: Chair Ad-Hoc EC Promotion and Tenure Procedures Review Subcommittee 2009-2012: Strategic Assessment Steering Committee (ACPE Accreditation and UM Provost’s Strategic Review)

2010-2011: Faculty Development Committee, Co-Chair

2009-2010: Ad-Hoc Basic Science Organizational Review Committee

1996-2008: Medicinal Chemistry Program WWW Site Webmaster

2005-2008: Medicinal Chemistry 1st Year Advisor & Recruitment Coordinator

2004-2008: Curriculum Committee

2005-2007: Ad-Hoc Faculty Evaluation and Development Committee

2003-2004: Academic Standing Committee

1999-2006: Chair-Computing Advisory Committee

1997-1999: Shared Research Equipment Committee

1996-2000: Pharm. D. Investigations Committee

1995-1999: Computer Resources Committee

1994-2001: Teaching Effectiveness Committee

1994-1999: "Bridge to a Ph. D." Program Committee

1994-1996: Publications Committee (1998-1999)

1995: Ad-Hoc Bylaws Review Subcommittee

1993-1998: Minority Summer High School Research Program, Faculty Mentor

2003-2004: Medicinal Chemistry Seminar Program Coordinator (1996-97, 2000-01)

1995-1996: College WWW Site Development Coordinator


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1991-2001: Committee on Diversity

PATENTS AND INVENTION DISCLOSURES • Garcia, G.A., Showalter, H.D., Rajeswaran, W., Lee, P., Franzblau, S.G., and Murakami, K., "

Novel Structure-Based Rifamycins for Drug-resistant TB and HIV Co-infection", Invention Disclosure (submitted 12/14/2018).

• Showalter, H.D., Garcia, G.A., Xu, H., Atwal, S.K., Nawarathne, I. and Kirchhoff, P.D., "Antimicrobial Compounds", WO 2013/086415 (2013). (provisional not pursued)

• Garcia, G.A., Showalter, H.D.H., and Brooks, A.F., "Syntheses of Radiolabelled PreQ1 and Queuine", submitted to UM Tech Transfer 8/8/2011, licensed to Moravek Biochemicals 9/14/11.

PUBLICATIONS RESEARCH PAPERS 1. Dirmaier, L.J., Garcia, G.A., Kozarich, J.W., and Kenyon, G.L., "Inhibition of Benzoylformate

Decarboxylase by p-Bromomethylbenzoylformate. Enzyme Catalyzed Modification of Thiamin Pyrophosphate by Halide Elimination and Tautomerization", Journal of the American Chemical Society (1986), 108, 3149-3150.

2. Weiss, P.M., Garcia, G.A., Kenyon, G.L., Cleland, W.W., and Cook, P.F., "Kinetics and Mechanism of Benzoylformate Decarboxylase Using 13C and Solvent Deuterium Isotope Effects on Benzoylformate and Benzoylformate Analogues", Biochemistry (1988), 27 (6), 2197-2205.

3. Reynolds, L.J., Garcia, G.A., Kozarich, J.W., and Kenyon, G.L., "Differential Reactivity in the Processing of p-Halomethylbenzoylformates by Benzoylformate Decarboxylase, a Thiamin Pyrophosphate-Dependent Enzyme", Biochemistry (1988), 27 (15), 5530-5538.

4. Garcia, G.A., Leatherbarrow, R.J., Eckstein, F., and Fersht, A.R., "Metal Ion Dependence of Phosphorothioate ATP Analogues in the Bacillus stearothermophilus Tyrosyl-tRNA Synthetase Reaction", Biochemistry (1990), 29 (6), 1643-1648.

5. Curnow, A.W., Kung, F.-L., Koch, K.A., and Garcia, G.A., "tRNA-Guanine Transglycosylase from Escherichia coli: Gross tRNA Structural Requirements for Recognition", Biochemistry (1993), 32 (19), 5239-5246.

6. Garcia, G.A., Koch, K.A., and Chong, S., "tRNA-Guanine Transglycosylase from Escherichia coli: Overexpression, Purification and Quaternary Structure", Journal of Molecular Biology (1993), 231, 489-497.

7. Avis, J.A., Day, A.G., Garcia, G.A., and Fersht, A.R., "The Reaction of Modified and Unmodified tRNATyr Substrates with Tyrosyl-tRNA Synthetase (Bacillus stearothermophilus)", Biochemistry (1993), 32 (20), 5312-5320.

8. Reuter, K., Chong, S., Ullrich, F., Kersten, H., and Garcia, G.A., "Serine-90 Is Required For Enzymic Activity By tRNA-Guanine Transglycosylase From Escherichia coli", Biochemistry (1994), 33 (23), 7041-7046.

9. Chong, S. and Garcia, G.A., "A Preferential PCR Method for Rapid and Highly Efficient Oligonucleotide-Directed Site-Specific Mutagenesis", BioTechniques (1994), 17 (4), 719-725.

10. Chong, S. and Garcia, G.A., "A Versatile and General Prokaryotic Expression Vector, pLACT7", BioTechniques (1994), 17 (4), 686-691.

11. Curnow, A.W. and Garcia, G.A., "tRNA-Guanine Transglycosylase from Escherichia coli:


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Recognition of Dimeric, Unmodified tRNATyr", Biochimie (1994), 76 (12), 1183-1191. 12. Chong, S., Curnow, A.W., Huston, T.J. and Garcia, G.A., "tRNA-Guanine Transglycosylase

from Escherichia coli is a Zinc-metalloprotein. Site-directed Mutagenesis Studies to Identify the Zinc Ligands", Biochemistry (1995), 34 (11), 3694-3701.

13. Curnow, A. W. and Garcia, G.A., "tRNA-Guanine Transglycosylase from Escherichia coli : Minimal tRNA Structure and Sequence Requirements for Recognition", Journal of Biological Chemistry (1995), 270 (29), 17264-17267.

14. Hoops, G.C., Townsend, L.B., and Garcia, G.A., "Studies of Heterocyclic Substrate and Analogues for tRNA-Guanine Transglycosylase from Escherichia coli", Biochemistry (1995), 34 (46), 15381-15387.

15. Hoops, G.C., Townsend, L.B., and Garcia, G.A., "Mechanism-based Inactivation of tRNA-Guanine Transglycosylase from Escherichia coli by 2-Amino-5-fluoromethylpyrrolo[2,3-d]pyrimidin-4(3H)-one", Biochemistry (1995), 34 (47), 15539-15544.

16. Garcia, G.A., Tierney, D.L., Chong, S., Clark, K., and Penner-Hahn, J.E., "X-Ray Absorption Spectroscopy of the Zinc Site in tRNA-Guanine Transglycosylase from Escherichia coli", Biochemistry (1996), 35 (9), 3133-3139.

17. Hoops, G.C., Park, J., Garcia, G.A., and Townsend, L.B., "Determination Of Acidic Ionization Constants Of Certain 5-Substituted 2-Aminopyrrolo[2,3-d]pyrimidin-4-ones". Journal of Heterocyclic Chemistry (1996), 33, 767-781.

18. Garcia, G.A. and Chong, S., "Cysteine 265 is in the Active Site of, but is not Essential for Catalysis by tRNA-Guanine Transglycosylase (TGT) from Escherichia coli ", Journal of Protein Chemistry (1997), 16 (1), 11-17.

19. Blake, R.E., O'Neil, J.R., and Garcia, G.A., "Oxygen Isotope Systematics of Biologically-Mediated Reactions of Phosphate I. Microbial Degradation of Organophosphorous Compounds", Geochimica et Cosmochimica Acta (1997), 61 (20), 4411-4422.

20. Kung, F.-L. and Garcia, G.A., "tRNA-Guanine Transglycosylase from Escherichia coli: The Effect of tRNA Primary Structure on Recognition", FEBS Letters (1998), 431, 427-432.

21. Blake, R.E., O'Neil, J.R., and Garcia, G.A., "Effects of microbial activity on the d18O of inorganic phosphate and textural features of synthetic apatities", American Mineralogist (1998), 83 (11-12, Part 2), 1516-1531.

22. Grädler, U., Ficner, R., Garcia, G.A., Stubbs, M.T., Klebe, G. and Reuter, K., "Mutagenesis and Crystallographic Studies of Zymomonas mobilis tRNA-Guanine Transglycosylase to Elucidate the Role of Serine 103 for Enzymatic Activity", FEBS Lett. (1999), 454 (1-2), 142-146.

23. Kung, F.-L., Nonekowski, S.T., and Garcia, G.A., "tRNA-Guanine Transglycosylase from Escherichia coli: Recognition of Noncognate-Cognate Chimeric tRNA and Discovery of a Novel Recognition Site Within the TYC Arm of tRNAPhe", RNA (2000), 6 (2), 233-244.

24. Grädler, U., Gerber, H.-D., Goodenough-Lashua, D.M., Garcia, G.A., Ficner, R., Reuter, K., Stubbs, M.T., and Klebe, G., "A New Target for Shigellosis: Rational Design and Crystallographic Studies of Inhibitors of tRNA-Guanine Transglycosylase", Journal of Molecular Biology (2001), 306, 455-467.

25. Nonekowski, S.T. and Garcia, G.A., "tRNA Recognition by the E. coli tRNA-Guanine Transglycosylase: the Role of U33 on U-G-U Sequence Recognition", RNA (2001), 7 (10), 1432-1441.


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26. Kittendorf, J.D., Barcomb, L.M., Nonekowski, S.T., and Garcia, G.A., "tRNA-Guanine Transglycosylase from E. coli: Molecular Mechanism and Role of Aspartate 89", Biochemistry (2001), 40 (47), 14123-14133.

27. Nonekowski, S.T., Kung, F.-L., and Garcia, G.A., "The E. coli tRNA-Guanine Transglycosylase Can Recognize and Modify DNA", Journal of Biological Chemistry (2002), 277(9), 7178-7182. PMID: 11751936

28. Brenk, R., Naerum, L., Graedler, U., Gerber, H.-D., Sohn, C., Garcia, G.A., Reuter, K., Stubbs, M.T., and Klebe, G. “Virtual Screening for Submicromolar Lead Inhibitors of TGT Based on a New Binding Mode Detected by Crystal Structure Analysis”, Journal of Medicinal Chemistry (2003), 46 (7), 1133-1143. PMID: 12646024

29. Brenk, R., Gerber, H.-D., Kittendorf, J.D., Garcia, G.A., Reuter, K., and Klebe, G. “From Hit to Lead: De Novo Design based on Virtual Screening Hits of Inhibitors of tRNA-guanine Transglycosylase, a Putative Target of Shigellosis Therapy”, Helvetica Chimica Acta (2003), 86, 1435-1452.

30. Goodenough-Lashua, D.M. and Garcia, G.A., “tRNA-Guanine Transglycosylase from Escherichia coli: a Ping-Pong Kinetic Mechanism is Consistent with Nucleophilic Catalysis”, Bioorganic Chemistry (2003), 31, 331-344. PMCID: 2784677

31. Heimbach, T., Rodriguez, N., Garcia, G.A., Oh, D.-M., and Fleisher, D., “Enzyme-mediated Precipitation of Parent Drugs from their Phosphate Prodrugs”, International Journal of Pharmaceutics (2003), 261 (1-2), 81-92.

32. Kittendorf, J.D., Sgraja, T., Reuter, K., Klebe, G., and Garcia, G.A., "An Essential Role for Aspartate 264 in Catalysis by tRNA-Guanine Transglycosylase from Escherichia coli", Journal of Biological Chemistry (2003), 278 (43), 42369-42376. PMID: 12909636

33. Brenk, R., Meyer, E., Reuter, K., Stubbs, M. T., Garcia, G.A., Diederich, F., and Klebe, G., "Crystallographic Study of Inhibitors of tRNA-Guanine Transglycosylase Suggests a New Structure-Based Pharmacophore for Virtual Screening", Journal of Molecular Biology (2004), 338 (1), 55-75.

34. Todorov, K.W., Tan, X.-J., Nonekowski, S.T., Garcia, G.A., and Carlson, H.A., “The Role of Aspartic Acid 143 in E. coli tRNA-Guanine Transglycosylase: Insights from Mutagenesis Studies and Computational Modeling”, Biophysical Journal (2005), 89 (3), 1965-1977. PMCID: 1366699

35. Todorov, K.W. and Garcia, G.A., "The Role of Aspartic Acid 143 in E. coli tRNA-Guanine Transglycosylase: Alteration of Heterocyclic Substrate Specificity”, Biochemistry (2006), 45 (2), 617-625. PMCID: 2533737

36. Hurt, J. K., Olgen, S. and Garcia, G.A., “Site-specific Modification of Shigella flexneri virF mRNA by tRNA-Guanine Transglycosylase In vitro”, Nucleic Acids Research (2007), 35, 4905-4913. PMCID: 1950534

37. Tidten, N., Stengl, B., Heine, A., Garcia, G.A., Klebe, G. and Reuter, K., “Glutamate Versus Glutamine Exchange Swaps Substrate Selectivity In tRNA-Guanine Transglycosylase: Insight Into The Regulation Of Substrate Selectivity By Kinetic And Crystallographic Studies”, Journal of Molecular Biology (2007) 374 (30), 764-776. PMCID: 2100405

38. Meyer, M.M., Roth, A., Chervin, S.M., Garcia, G.A., and Breaker, R.R., “Confirmation of a second natural preQ1 aptamer in Streptococcaceae bacteria”, RNA (2008) 14, 685-695. PMCID: 2271366

39. Garcia, G.A., Chervin, S.M., and Kittendorf, J.D., “Identification of the Rate-Determining Step of


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tRNA-Guanine Transglycosylase from E. coli”, Biochemistry (2009), 48(47), 11243-51. PMCID: 2789984

40. Hurt, J.K., McQuade, T., Emanuele, A.A., Larsen, M. and Garcia, G.A., “HTS of the Virulence Regulator VirF: a Novel Antibiotic Target for Shigellosis”, Journal of Biomolecular Screening (2010), 15, 379-387. PMCID: 2882805

41. Chen, Y.-C., Kelly, V.P., Stachura, S., and Garcia, G.A., “Characterization of the Human tRNA-Guanine Transglycosylase: Confirmation of the Heterodimeric Subunit Structure”, RNA (2010), 16(5), 958-968. PMCID: 2856889

42. Brooks, A.F., Garcia, G.A., and Showalter, H.D.H., “A Short, Concise Synthesis of Queuine”, Tetrahedron Letters (2010), 51, 4163-4165.

43. Chen, Y.-C., Brooks, A.F., Goodenough-Lashua, D.M., Kittendorf, J.D., Showalter, H.D.H. and Garcia, G.A., “Evolution of tRNA-Guanine Transglycosylase: Insight Gained from Studies of Heterocyclic Substrate Recognition by the Human and E. coli tRNA-Guanine Transglycosylases”, Nucleic Acids Research (2011), 39 (7), 2834-2844, PMCID: 3074131

44. Gill, Sumandeep K. and Garcia, G.A., “Rifamycin inhibition of WT and Rif-resistant Mycobacterium tuberculosis and Escherichia coli RNA polymerases in vitro”, Tuberculosis (2011), 91 (5), 361-369, PMID: 21704562

45. Thomas, C.E., Chen, Y.-C. and Garcia, G.A., “Differential Heterocyclic Substrate Recognition by, and Pteridine Inhibition of E. coli and Human tRNA-Guanine Transglycosylases”, Biochemical Biophysical Research Communications (2011), 410 (1), 34-39, PMCID: 3124622.

46. Jin, Y., Gill, S.K., Kirchhoff, P.D., Wan, B., Franzblau, S.G., Garcia, G.A., and Showalter, H.D.H., “Synthesis and Structure-Activity Relationships of Novel Substituted 8-Amino-and 1,8-Pyrazole Congeners of the Antituberculars Rifamycin S and Rifampin”, Bioorganic and Medicinal Chemistry Letters (2011), 21, 6094-6099, PMID: 21903392.

47. Gill, S.K., Hao, X., Kirchhoff, P.D., Wan, B., Zhang, N., Peng, K.-W., Franzblau, S.G., Garcia, G.A., and Showalter, H.D.H., “Structure-based Design of Novel Benzoxazinorifamycins with Potent Binding Affinity to Wild-type and Rifampin-resistant Mutant Mycobacterium Tuberculosis RNA Polymerases”, Journal of Medicinal Chemistry (2012), 55 (8), 3814–3826, PMID: 22452568 [PubMed - as supplied by publisher]

48. Lee, T.-Y., Park, Y.S., Garcia, G.A., Sunahara, R.K., Woods, J.H., and Yang, V.C., “Cell Permeable Cocaine Esterases Constructed by Chemical Conjugation and Recombinant Biology”, Molecular Pharmaceutics (2012), 9 (5), 1361-1373, PMID: 22404344 [PubMed - as supplied by publisher]

49. Brooks, A.F., Vélez-Martínez, C. S., Showalter, H.D.H., and Garcia, G.A. “Investigating the Prevalence of Queuine in Escherichia coli RNA via Incorporation of the Tritium-labeled Precursor, PreQ1“, Biochemical Biophysical Research Communications (2012), 425 (1), 83-88, PMID: 22819844 [PubMed - in process]

50. *Molodtsov, V., Nawarathne, I.N., Scharf, N.T., Kirchhoff, P.D., Showalter, H.D.H., Garcia, G.A., and Murakami, K.S., “X-ray crystal structures of the Escherichia coli RNA polymerase in complex with benzoxazinorifamycins”, Journal of Medicinal Chemistry (2013), 56 (11), 4758-4763, PMCID: 3745299 *Note that this paper (#50) was highlighted on the cover of this issue.

51. Emanuele, A.A., Praskievicz, N.A., Chen, Y.-C., Maurelli, A.T., and Garcia, G.A., “Potential Novel Antibiotics from HTS Targeting the Virulence-regulating Transcription Factor, VirF, from


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Shigella flexneri”, Journal of Antibiotics (2014), 67, 379-386, PMCID: 4050983. Note that this paper was featured on the journals webpage for this issue and was noted as #1 of the top 10 articles downloaded in the 15 days since the issue was published.

52. Beleh, M., Engels, M. and Garcia, G.A., “Integrating Basic Sciences in the Pharm. D. Curriculum: A New Medicinal Chemistry and Pharmacology Course Sequence”, American Journal of Pharmaceutical Education (2015), 79 (1), Article 13.

53. Emanuele, A.A. and Garcia, G.A., “Mechanism of Action and Initial, In vitro SAR of an Inhibitor of the Shigella flexneri Virulence Regulator VirF”, PLOS One (2015), e0137410 PMCID: PMC4564171.

54. Scharf, N.T., Kontos, A., Molodtsov, V., Murakami, K.S., and Garcia, G.A., “Novel Chemical Scaffolds for Inhibition of Rifamycin-Resistant RNA Polymerase Discovered from High-Throughput Screening”, SLAS Discovery (2017), 22 (3), 287-297, PMCID: PMC5323270.

55. Molodtsov, V., Scharf, N.T., Stefan, M.A., Garcia, G.A., and Murakami, K.S., “Structural Basis for Rifampin-Resistance of Bacterial RNA Polymerase by Clinically Important Rifampin Resistant Mutations”, Molecular Microbiology (2017), 103 (6), 1034-1045, PMCID: PMC5344776.

56. Stefan, M.A., Ugur, F.S., and Garcia, G.A., “Source of the Fitness Defect in Rifamycin-Resistant M. Tuberculosis RNA Polymerase and the Mechanism of Compensation by Secondary Mutations”, Antimicrobial Agents and Chemotherapy (2018), 62 (6), e00164-18, 1-13.

57. Stefan, M.A., Velazquez, G.M., Lee, P., Gallagher, E., and Garcia, G.A., “High-throughput Screening to Identify Inhibitors of the Interaction Between the M. tuberculosis Transcriptional Regulator CarD and RNA Polymerase (CarD•RNAP)”, In preparation.

BOOK CHAPTERS, REVIEW PAPERS AND COMMENTARIES

1. Kenyon, G.L. and Garcia, G.A., "Design of Kinase Inhibitors", Medicinal Research Reviews (1987), 7 (4), 398-416.

2. Garcia, G.A. and Chong, S., "Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7", in "Recombinant Gene Expression Protocols", Methods in Molecular Biology (1997), 62, 63-71.

3. Garcia, G.A. and Goodenough-Lashua, D.M., "Mechanisms of RNA-Modifying and -Editing Enzymes ", in Modification and Editing of RNA: The Alteration of RNA Structure and Function, Grosjean, H. and Benne, R. Eds., ASM Press (1998).

4. Garcia, G.A. and Goodenough-Lashua, D.M., "Appendix III: General Properties of RNA-Modifying and -Editing Enzymes", in Modification and Editing of RNA: The Alteration of RNA Structure and Function, Grosjean, H. and Benne, R. Eds., ASM Press (1998).

5. Garcia, G.A. and Kittendorf, J.D., “Transglycosylation, a Mechanism for RNA Modification (and Editing?)”, Bioorganic Chemistry (2005), 33 (3), 229-251.

6. Chervin, S.M., Kittendorf, J.D., and Garcia, G.A., “Probing the Intermediacy of Covalent RNA×Enzyme Complexes in RNA Modification Enzymes”, Methods in Enzymology (2007), 425, 121-137. PMCID: 2800168

7. Garcia, G.A., Hurt, J.K., and Chen, Y.-C., “RNA Modifying Enzymes”, invited chapter in Comprehensive Natural Products II: Chemistry and Biology, Volume 6: Carbohydrates, Nucleosides and Nucleic Acids, Mander, L and Lui, H.-W. Eds., Elsevier (2010).

8. Aristoff, P.A., Garcia, G.A., Kirchhoff, P.D., and Showalter, H.D.H., “Rifamycins – Obstacles and


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Opportunities”, Tuberculosis (2010), 90, 94-118. PMID: 20236863 9. Garcia, G.A., “Response to: Viewpoint – Point/Counterpoint: Should a Pharmacy Dean be a

Pharmacist?”, American Journal of Pharmaceutical Education (2015), 79 (9), Article 144. *Note that this commentary (#9) was highlighted in Pharmacy Times on Jan. 21, 2016.

10. Woods, T.M., Acosta, W.R., Chisholm-Burns, M., Chung, E.P., Cox, A. G., Garcia, G.A., and Klucken, J.R., “Academic Freedom Should Be Redefined: Point and Counterpoint”, American Journal of Pharmaceutical Education (2016), 80 (9), Article 146.

SELECTED ABSTRACTS (poster presentations from 2000, presenter underlined)

1. Goodenough-Lashua, D.M. and Garcia, G.A., "Kinetic Analyses of a tRNA-Guanine Transglycosylase Mutant with Enhanced Activity", ASBMB & ASPET Annual Meeting, June 4-8, 2000, Boston, Massachusetts.

2. Thomas, C.E. and Garcia, G.A., "Differential Substrate Recognition by tRNA-Guanine Transglycosylases", ASBMB & ASPET Annual Meeting, June 4-8, 2000, Boston, Massachusetts.

3. Goodenough-Lashua, D.M. and Garcia, G.A., "Role of Cysteine 145 in Eubacterial tRNA-Guanine Transglycosylase", 17th Enzyme Mechanisms Conference, January 3-7, 2001, Marco Island, Florida.

4. Thomas, C.E. and Garcia, G.A., "A Series of Amino Acid Substitutions That Confer Eukaryotic-Type Activity to E. coli TGT", “Experimental Biology 2002”,the FASEB Annual Meeting, April 20-24, 2002, New Orleans, LA.

5. Garcia, G.A. and Thomas, C.E., "Heterocyclic Substrate Recognition by tRNA-Guanine Transglycosylases from Human and Eubacteria", "RNA 2002", the Seventh Annual Meeting of the RNA Society, May 30-June 5, 2002, Madison, WI.

6. Kittendorf, J.D. and Garcia, G.A., "Examining the Role of Aspartate 264 in Catalysis by tRNA Guanine Transglycosylase", "RNA 2002", the Seventh Annual Meeting of the RNA Society, May 30-June 5, 2002, Madison, WI.

7. Garcia, G.A., Kittendorf, J.D. and Abold, K.W., "Catalytic Mechanism of tRNA-Guanine Transglycosylase: A Tale of Three Aspartates", 18th Enzyme Mechanisms Conference, January 4-7, 2003, Galveston Island, TX.

8. Garcia, G.A. and Thomas, C.E., "Differential Heterocyclic Substrate Recognition by tRNA-Guanine Transglycosylases", "RNA 2004", the Ninth Annual Meeting of the RNA Society, June 1-6, 2004, Madison, WI.

9. Chervin, S.M., Kittendorf, J.D. and Garcia, G.A., "Understanding the Rate-determining Step in the Kinetic Pathway of Eubacterial tRNA-Guanine Transglycosylase", 20th Enzyme Mechanisms Conference, January 3-7, 2007, St. Petersburg Beach, FL.

10. Chen, Y.-C. and Garcia, G.A., "Recognition of Cognate tRNAs by the Human tRNA-Guanine Transglycosylase", 223rd ACS National Meeting, March 25-29, 2007, Chicago, IL.

11. Chervin, S.M. and Garcia, G.A., "Efforts Toward the Isolation and Sequence Determination of the Putative Mammalian Enzyme Carbon-Mannosyltransferase", 223rd ACS National Meeting, March 25-29, 2007, Chicago, IL.

12. Chervin, S.M., Kittendorf, J.D. and Garcia, G.A., "Understanding the Rate-determining Step in the Kinetic Pathway of Eubacterial tRNA-Guanine Transglycosylase", 223rd ACS National


11

Meeting, March 25-29, 2007, Chicago, IL. 13. Cutcher, J.K. and Garcia, G.A., "Modification of an mRNA Involved in Bacterial Virulence by

tRNA-Guanine Transglycosylase", 223rd ACS National Meeting, March 25-29, 2007, Chicago, IL. 14. Lee, K.-H. and Garcia, G.A., "Regulation of Human tRNA-Guanine Transglycosylase:

Interactions of Catalytic and Regulatory Subunits", 223rd ACS National Meeting, March 25-29, 2007, Chicago, IL.

15. Brooks, A.F., Garcia, G.A., and Showalter, H.D.H., “Synthesis of Tritium Labeled Queuine, PreQ1 and Related Azide Probes for Determining the Prevalence of Queuine in RNA”, 32nd National Medicinal Chemistry Symposium, June 6-9, 2010, Minneapolis, MN.

16. Chen, Y.-C., Brooks, A.F., Goodenough-Lashua, D.M., Showalter, H.D.H. and Garcia, G.A., “Evolution of tRNA-Guanine Transglycosylase: Insight Gained from Studies of Heterocyclic Substrate Recognition by the Human and E. coli tRNA-Guanine Transglycosylases”, 2010 Midwest Enzyme Chemistry Conference, October 16, 2010, Northwestern University, Evanston, IL.

17. Gill, S.K. and Garcia, G.A., “Cloning, Expression in E. coli and Characterization of Wild-type and Rifamycin Resistant Mutants of M. tuberculosis RNA Polymerase”, 2010 Midwest Enzyme Chemistry Conference, October 16, 2010, Northwestern University, Evanston, IL.

18. Velez, C.S., Brooks, A.F., Showalter, H.D.H. and Garcia, G.A., “Determining the Prevalence of Queuine in Escherichia coli RNA”, 2010 Annual Biomedical Research Conference for Minority Students (ABRCMS), November 10-13, 2010, Charlotte, NC.

19. Brooks, A.F., Velez, C.S., Showalter, H.D.H. and Garcia, G.A., “Investigating the Prevalence of Queuine in Escherichia coli RNA via Incorporation of Tritium Labeled Precursor, PreQ1”, 241st ACS National Meeting & Exposition, March 27- 31, 2011. Anaheim, CA. (poster and oral presentation)

20. Brooks, A.F., Showalter, H.D.H. and Garcia, G.A., “Evaluation of Azide Congeners of PreQ1 as Substrates for tRNA Guanine Transglycosylase”, 241st ACS National Meeting & Exposition, March 27- 31, 2011. Anaheim, CA.

21. Gill, S.K., Showalter, H.D.H. and Garcia, G.A., “Characterization of rifamycin-resistant Mycobacterium tuberculosis RNA polymerases”, Experimental Biology 2012, April 21- 25, 2011, San Diego, CA. (poster and oral presentation)

22. Emanuele, A.A., Praskievicz, N.A., Chen, Y.-C., Maurelli, A.T., and Garcia, G.A., “Potential Novel Antibiotics from HTS Targeting the Virulence-regulating Transcription Factor, VirF, from Shigella flexneri”, Fusion Conferences: Drug Discovery Re-Invented, October 16-19, 2013, Scottsdale, AZ.

23. Ragazzone, N.J., Emanuele, A.A. and Garcia, G.A., “Development of Novel Inhibitors of the Key Shigella flexneri Virulence Regulator", 35th National Medicinal Chemistry Symposium, 6/26-29/2016, Chicago, IL.

24. Garcia, G.A., Scharf, N.T., Ashkar, S.R., Rajeswaran, R., Showalter, H.D.H., and White, A., “Antibiotic Discovery for Tuberculosis: Returning to a “Golden Target”, RNA Polymerase”, Tuberculosis Drug Discovery & Development Gordon Research Conference, 6/25 - 6/30/2017, Renaissance Tuscany Il Ciocco in Lucca (Barga) Italy.

25. Stefan, M.A. and Garcia, G.A., “Source of the Fitness Defect in Rifamycin-Resistant M. tuberculosis RNA Polymerase and the Mechanism of Compensation by Secondary Mutations”,


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FASEB Summer Conference: Mechanism and Regulation of Prokaryotic Transcription, 6/25-30. 2017, Saxtons River, VT.

26. Garcia, G.A., Scharf, N.T., Ashkar, S.R., Rajeswaran, R., Showalter, H., and White, A., “Antibiotic Discovery for Tuberculosis: Returning to a “Golden Target”, RNA Polymerase”, MichBio Symposium, 10/10/2017, Wayne State University, Detroit, MI.

27. Albert, J., Chang, R.S., Giles, M., Garcia, G.A., and Schwendeman, S.P., “Utilizing Zinc – His-tag coordination for remote loading of biomacromolecules into self-healing PLGA microparticles without organic solvent”, Controlled Release Society, 7/21-24/2019, Palacio de Congresos de Valencia, Spain.

28. Ragazzone, N.J. and Garcia, G.A., “Evaluating the VirF DNA Binding Domain as an Anti-Virulence Target”, Mechanisms of Microbial Transcription Gordon Research Conference, 7/28-8/2, 2019, Bates College, Lewiston, ME.

29. Guild, K.R., Stefan, M.A. and Garcia, G.A., “Exploration of Mycobacterium tuberculosis RNA polymerase's putative ppGpp binding site as a potential therapeutic target”, ACS National Meeting, 8/25-29/2019, San Diego, CA.

30. Ashkar, S.R., Rajeswaran, W., Showalter, H.D.H., White, A., Sherman, D.H., Tripathi, A. and Garcia, G.A., “Development of novel inhibitors for Rifamycin-resistant RNA polymerase of Mycobacterium tuberculosis”, Tuberculosis Drug Discovery & Development Gordon Research Conference, 7/7-12/19, Castelldefels, Spain.

31. Ashkar, S.R., Sherman, D.H., Tripathi, A. and Garcia, G.A., “Exploring Natural Products for Novel Inhibitors of Rifamycin-resistant RNA Polymerase of Mycobacterium tuberculosis”, New Frontiers in Natural Products Discovery – Corteva Agriscience, 8/7-9/19, Indianapolis, IN.

SELECTED INVITED LECTURES

5/4-8/94 EMBO Workshop on Nucleotide Modification and Base Conversion of RNA, Aussois, France. "Investigations Into the Structure and Substrate Recognition of tRNA-Guanine Transglycosylase (TGT) from Escherichia coli"

1/4-8/95 14th Enzyme Mechanisms Conference, Scottsdale Arizona "Post-transcriptional Modification of tRNA with Queuine" 2/17/95 University of Puerto Rico-Mayagüez, Department of Chemistry, "Post-transcriptional Modification of tRNA with Queuine" 5/27-6/1/95 16th International tRNA Workshop, Madison, Wisconsin. "tRNA-Guanine Transglycosylase from Escherichia coli" 6/30/96 FASEB Summer Conference, Copper Mountain, Colorado. “tRNA Recognition by and Mechanism of tRNA-Guanine Transglycosylase" 5/12/97 California State University, Sacramento, Department of Chemistry. "Mechanism-based Inhibition of tRNA-Guanine Transglycosylase, a tRNA Modifying

Enzyme" 12/15/97 Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Gif-sur-Yvette, France. “tRNA Recognition by and Mechanism of tRNA-Guanine Transglycosylase from

Escherichia coli" 12/18/97 Philipps-Universitaet Marburg, Max-Planck-Institute for Microbiology, Germany.


13

"Post-transcriptional Modification of tRNA with Queuine" 5/17-21/98 98th General Meeting of the American Society for Microbiology, Atlanta, Georgia. "RNA Modification via Transglycosylation" 4/12/00 Wichita State University, Department of Chemistry. "Post-transcriptional Modification of tRNA via Transglycosylation" 5/8/00 University of the Pacific, School of Pharmacy. "Post-transcriptional Modification of tRNA via Transglycosylation" 9/29/01 5th Annual Michigan RNA Society Meeting, Wayne State University. "The E. coli tRNA-Guanine Transglycosylase Can Recognize and Modify DNA" 11/26/01 Washington State University, Department of Chemistry. "Mechanism-based Inhibition of tRNA-Guanine Transglycosylase, A Potential New

Antibiotic Target" 8/22/02 Western University of the Health Sciences, College of Pharmacy "tRNA-Guanine Transglycosylase, A Potential Novel Antibiotic Target" 1/16/03 Ohio State University, Department of Microbiology "tRNA-Guanine Transglycosylase, A Potential Novel Antibiotic Target" 1/17/03 University of Dayton, Department of Chemistry "Mechanism-Based Inhibition of tRNA-Guanine Transglycosylase, a Potential Novel

Antibiotic Target" 10/5/04 University of Iowa, Division of Medicinal and Natural Products Chemistry. "Nucleotide Modification by tRNA-Guanine Transglycosylase: A Tale of Three

Aspartates" 5/2/05 University of Tennesee, Memphis, Department of Molecular Sciences. "Nucleotide Modification by tRNA-Guanine Transglycosylase: A Tale of Three

Aspartates" 10/26/2007 3rd International Meeting on Medicinal and Pharmaceutical Chemistry, Antayla, Turkey. "Targeting the Regulation of Virulence Gene Expression as a Novel Antibiotic

Approach" 3/21/2008 Albion College Department of Chemistry. "Targeting the Regulation of Virulence Gene Expression as a Novel Antibiotic

Approach" 4/2/2008 Wayne State University, School of Pharmacy. "Targeting the Regulation of Virulence Gene Expression as a Novel Antibiotic

Approach" 3/21/2014 University of Michigan-Dearborn, College of Arts, Sciences and Letters. "Antibiotic Drug Discovery for “Neglected” Diseases" 3/4/2015 Michigan State University, Department of Chemistry. "Drug Discovery for Tuberculosis” 3/6/2015 Butler University, Department of Chemistry. "Drug Discovery for Tuberculosis” 9/9/2016 University of Michigan, Center for the Discovery of New Medicines. "Antibiotic Discovery for Tuberculosis: Returning to a “Golden Target”, RNA


14

Polymerase” 3/1/17 National Taiwan University, School of Pharmacy, Taiwan. "Antibiotic Discovery for Tuberculosis: Returning to a “Golden Target”, RNA

Polymerase” 3/3/17 National Yang-Ming University, School of Pharmacy, Taiwan. "Antibiotic Discovery for Tuberculosis: Returning to a “Golden Target”, RNA

Polymerase” 7/7-12/19 2019 Gordon Research Conference on Tuberculosis Drug Discovery & Development,

Castelldefels, Spain "Novel Approaches to Targeting M. tuberculosis RNA Polymerase” FUNDING Active:

US Public Health Service, National Institutes of Health (PI, 1R01AI110780-01-A1): "Novel Structure-Based Rifamycins for Drug-resistant TB and HIV Co-infection", 8/1/15-

1/31/2020 ($2,186,473 total direct costs) Tres Cantos Open Lab Foundation, GSK (PI) “Targeting the Virulence Regulator VirF, a Novel Approach to Antibiotics for Shigellosis”,

11/1/18 – 5/31/20 ($108,600 total costs) Submitted:

US Public Health Service, National Institutes of Health (PI, 1R01AI110780-06): "Novel Structure-Based Rifamycins for Drug-resistant TB and HIV Co-infection", 2/1/20-

1/31/2025 ($??? total direct costs) Michigan–Israel Partnership for Research and Education Collaborative Research Grant, Letter of

Intent (co-PI, PI Serge Ankri, Technicon Institute, Israel): "Determining if EhTRTD1 is the queuine salvage enzyme in the protozoan parasite

Entamoeba histolytica", 9/1/19-8/31/21 ($100,000 total costs) Previous:

US Public Health Service, National Institutes of Health (PI through 6/30/16, co-PI through 12/31/17, 2T32GM007767-37):

"Interdepartmental Training in Pharmacological Sciences", 7/1/15-6/30/2020 ($4,215,390 total direct costs)

UM Center for the Discovery of New Medicines (PI) “Identification of Novel Inhibitors of MTB RNA Polymerase via HTS”, 9/1/14 – 8/31/2015

($25,000)

College of Pharmacy, Vahlteich/UpJohn Research Award (PI) “Bridging Support”, 9/1/14 – 4/1/2015 ($40,000)

US Public Health Service, National Institutes of Health (PI, 1R21AI085179-01A1): "Targeting Bacterial Virulence Transcription Factors, a Novel Antibiotic Approach", 09/1/10

– 8/31/13 ($275,000 total direct costs)


15

College of Pharmacy, Vahlteich/UpJohn Research Award (PI) “Bridging Support”, 8/1/10 – 7/31/2011 ($19,500)

US Public Health Service, National Institutes of Health (PI, R01-GM65489-A1): "Mechanism and Differential Substrate Recognition of a tRNA-Modifying Enzyme", 8/01/03

- 12/31/07 ($800,000 total direct costs)

College of Pharmacy, Vahlteich/UpJohn Research Award (co-PI w/ S. Chervin): "Isolation, Subcloning and Overexpression of Mammalian Carbon-Mannosyltransferase",

9/1/06 - 12/31/07 ($40,000 total direct costs)

University of Michigan, College of Pharmacy, Vahlteich/Upjohn Research Award (PI) "Physiological Role(s) of Queuine", 4/1/02 - 3/31/03 ($40,000)

National Science Foundation: (co-I, D. Lubman PI): "MALDI-TOF-MS.", 4/01/2001 - 03/31/2002 ($162,900)

Eli Lilly and Company Foundation: (co-I, F. Ascione PI): "Creating a Partnership for Promoting Industry-Academia Relations: The Eli Lilly Academic

Technology Enhancement Program in the Pharmaceutical Sciences at the University of Michigan, College of Pharmacy", submitted 9/01/2000, awarded 1/26/2001 ($500,000)

National Science Foundation (PI, MCB-9720139): "Mechanism & Molecular Recognition of a tRNA Modification", 9/1/97 - 8/31/01 ($227,463)

US Public Health Service, National Institutes of Health (co-I, R25-GM-51793, V. Perry PI): "Bridging the Underrepresented Minority Gap: M.S. to Ph.D.", 09/30/94 - 08/31/99

($306,000)

National Science Foundation (PI, supplement to MCB-9720139): "Mechanism & Molecular Recognition of a tRNA Modification", 8/1/98 - 7/31/99 ($12,000 for

instrument replacement)

University of Michigan, College of Pharmacy, Upjohn Research Award (PI) "Mechanism & Molecular Recognition of a tRNA Modification", 7/1/98 - 6/30/99 ($25,000)

US Public Health Service, National Institutes of Health FIRST Award (PI, R29-GM45968): "Mechanism & Molecular Recognition of a tRNA Modification", 4/1/92 - 3/31/98 ($350,000)

US Public Health Service, National Institutes of Health Shannon Award (PI, R55-GM45968): "Mechanism & Molecular Recognition of a tRNA Modification", 9/1/91 - 3/31/92 ($36,500)

University of Michigan, Rackham Graduate School/Office of the Vice-President for Research (PI) Research Partnership Award (R. E. Blake), 9/1/95 - 8/31/96 (ca. $15,500)

University of Michigan, College of Pharmacy, Vahlteich Research Award (PI) "Queuosine Biosynthesis: Attachment of Cyclopentyl Moiety", 9/1/94 - 8/31/95 ($24,934)

University of Michigan, Rackham Graduate School (PI) Minority Faculty Development Fund Award, 7/1/93 - 6/30/94 ($13,062)

University of Michigan, College of Pharmacy, Upjohn Research Award (PI)


16

"Cloning and Expression of tRNA-Guanine Transglycosylase", 7/1/90 - 8/31/91 ($25,000) TEACHING (Courses, taught in conjunction with other faculty) 2016-present: Medicinal Chemistry 510: Principles of Drug Action II (21.5 lecture hours, course

coordinator) Pharm. D. course. Lecture material covers: adrenergics, cholinergics, histaminergics, NSAIDS, anti-coagulants, vitamins, and lipid-lowering agents. Course is integrated with Pharmacology.

2011-2015: Medicinal Chemistry 510 (previously 415): Principles of Drug Action I (12.5 lecture hours, course coordinator) Pharm. D. course. Lecture material covers physiochemical properties and drug action, receptor binding, ADME, general adrenergics and general cholinergics. Substantially revised course integrating Pharmacology.

2015: Medicinal Chemistry 600: Principles of Drug Action II (11 lecture hours) Pharm. D. course. Lecture material covered "Central Nervous System Agents" including: anesthetics, sedative-hypnotics, anticonvulsants, anxiolytics, neuroleptics, migraine, opioids, and CNS stimulants. Substantially revised course integrating Pharmacology.

2009-2010: Medicinal Chemistry 410: Principles of Medicinal Chemistry (20 lecture hours) Pharm. D. course. Lecture material covered physiochemical properties and drug action" and peripheral nervous system agents" including: local anesthetics, cholinergics, adrenergics and anti-histamines.

1994-present: Medicinal Chemistry 532: Bioorganic Principles of Medicinal Chemistry (6-12 lecture hours and course coordinator 1994-2009) Ph. D. graduate course. Lecture material has covered "Receptors", "Nucleic Acids as Drug Targets" and "Drug Metabolism", currently “Nucleic Acids”.

1991-2007: Medicinal Chemistry 410: Principles of Medicinal Chemistry (20 lecture hours, half of course) Pharm. D. course. Lecture material covered "Drug Metabolism" and "Central Nervous System Agents" including: anesthetics, sedative-hypnotics, anticonvulsants, anxiolytics, neuroleptics, neuromusculars, opioids, and CNS stimulants.

Winter 2006: Chemical Biology: Chemical Biology II (14 lecture hours, 1/3 of course) Ph. D. graduate course. Lecture material covered "Protein Homeostasis".

2000-01, Medicinal Chemistry 550 / Pharmacology 504: Responsible Conduct of Research 2014-15: and Seminars in Pharmacological Sciences (course co-coordinator)

Ph. D. graduate course. This course involves research ethics presentations and discussions alternating with individual research presentations by PSTP trainees, meeting on a monthly basis.

1998 & 2000, Medicinal Chemistry 740: Seminar in Medicinal Chemistry (individual) 2003-06: Ph. D. graduate course. Students research a topic from the literature and give a

seminar on it. The coordinator guides the students through the process, administratively manages the evaluation and feedback process, and assigns a grade for the course.


17

1991-96: Pharmacognosy 412: Principles of Pharmacognosy (7.5 lecture hours) Pharm. D. course. Lecture material covered "Antibiotics and the cytoplasmic membrane" (1.5 hrs) and "Inhibitors of Nucleic Acid Metabolism" (6 hrs).

Spring 1992: Pharmaceutics 755: Protein/Peptide Biotechnology (3 lecture hours) Ph. D. graduate course. Lecture material covered "Protein/Peptide Synthesis (Genetic Engineering Approaches)".

Ph. D. DISSERTATION ADVISOR (21) Current (5)

Ashkar, Shireen Medicinal Chemistry (matriculated Fall 2014) Dow, Garrett Medicinal Chemistry (matriculated Fall 2017) Guild (Harms), Katherine Medicinal Chemistry (matriculated Fall 2015) Ragazzone, Nicholas Medicinal Chemistry (matriculated Fall 2014) Velazquez, Glory Medicinal Chemistry (matriculated Fall 2017)

Completed (16)

Atwal (Gill), Chemical Biology (2012) Sumandeep “In vitro Characterization of Wild-type and Rifamycin-resistant

Mycobacterium tuberculosis RNA polymerases” Present Position: Scientist and Project Manager of IND-enabling Program, Carmot Therapeutics, Inc.

Brooks, Medicinal Chemistry (2012), Co-mentor with Prof. Hollis Showalter Allen “Synthesis of Tritium Labeled Queuine, PreQ1 and Related Azide Probes Toward Examining the Prevalence of Queuine” Present Position: Research Investigator, University of Michigan, MI Chen, Medicinal Chemistry (2011) Yi-Chen “Evolution of Eukaryal tRNA-Guanine Transglycosylase: Insight Gained

from the Characterization of the Human and Escherichia coli tRNA-Guanine Transglycosylases”

Present Position: Pharmacist, Ann Arbor, MI Chong, Pharmacognosy (1995) Shaorong “Characterization of tRNA-Guanine Transglycosylase from Escherichia

coli" Present Position: Director of Protein Biology and Biochemistry, Arbor Biotechnologies, Boston, MA

Curnow, Medicinal Chemistry (1995) Alan “Recognition of tRNA Analogues by the Post-transcriptional

Hypermodification Enzyme: tRNA-Guanine Transglycosylase" Present Position: Pharmacist, St. John Providence, Providence Park

Hospital, Novi, MI


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Emanuele, Medicinal Chemistry (2015) Anthony “An Antibiotic Discovery Campaign Targeting VirF, the Main

Transcriptional Regulator of Virulence in Shigella flexneri”, Present Position: Senior Development Scientist, Beckman-Coulter, Novi, MI

Goodenough-Lashua, Medicinal Chemistry (2001) DeeAnne “Mechanistic Studies of the Reaction Catalyzed by the Eubacterial tRNA-

Guanine Transglycosylase" Present Position: Associate Teaching Professor, Department of Chemistry

and Biochemistry, University of Notre Dame, South Bend, IN Hoops, Medicinal Chemistry (1995) Geoffrey “Studies of the Heterocyclic Substrate for the tRNA-Guanine

Transglycosylase from Escherichia coli" Present Position: Professor, Chemistry Dept., Butler University,

Indianapolis, IN Hurt (Cutcher), Medicinal Chemistry (2009) Julie “Modulation of Expression and Antibacterial Targeting of Shigella Flexneri

VirF” Present Position: Medical Writer, Whitsell Innovations, Chapel Hill, NC Kittendorf, Medicinal Chemistry (2004) Jeffrey “Catalysis by tRNA-Guanine Transglycosylase from Escherichia coli"

Present Position: Co-Founder and Managing Partner, PharmaForensics Laboratories and Co-Founder & Vice President, Alluvium Biosciences Inc., Ann Arbor, MI

Kung, Medicinal Chemistry (1998) Fan-Lu “Investigation of the tRNA Recognition of tRNA-Guanine Transglycosylase

from Escherichia coli" Present Position: Associate Professor, School of Pharmacy, National

Taiwan University, Taipei, Taiwan Nonekowski, Medicinal Chemistry (2001) Susanne “Exploring the Limits of tRNA Substrate Recognition by Escherichia coli

tRNA-Guanine Transglycosylase" Present Position: Distinguished University Lecturer, College of Pharmacy

and Pharmaceutical Sciences, University of Toledo, Toledo, OH Scharf, Medicinal Chemistry (2017) Nathan “Identification and Evaluation of Bacterial RNA Polymerase Inhibitors

Using a Novel Plasmid-based Transcription Assay” Present Position: Visiting Professor, University of Tampa, FL Stefan, Medicinal Chemistry (2018) Maxwell “Kinetic Characterization of Rifamycin-Resistant M. tuberculosis RNA

Polymerases and Novel Therapeutic Approach for Targeting Transcription”

Present Position: Postdoc, Sandia National Labs, Livermore, CA


19

Thomas, Medicinal Chemistry (2004) C. Eric “A Comparison of the Active Sites of Eukaryotic and Bacterial tRNA-

Guanine Transglycosylases” Present Position: Associate Director, LC-MS Scientific Lead, Covance Inc., Indianapolis, IN

Todorov (Abold), Medicinal Chemistry (2003) Katherine “Biochemical and Computational Exploration of Heterocyclic Substrate

Recognition and Discrimination by Eubacterial tRNA Guanine Transglycosylase”

Present Position: Lieutenant Colonel USAF, Springfield, VA POSTDOCTORAL ADVISOR

Chen, Yi-Chen Jan. 2011-Aug. 2011 Chervin, Stephanie Sept. 2005-Dec. 2007 Emanuele, Anthony Jan. 2016-June 2016 Lee, Kyung-Hoon Oct. 2005-August 2007 Nawarathne, Irosha May 2012-August 2014 Olgen, Sureyya Nov. 2003-Oct. 2005 Stefan, Maxwell July-August 2018

Ph. D. DISSERTATION COMMITTEE MEMBER (68 total) Current

Shenoy, Vikram Medicinal Chemistry (Amidon) Shergalis, Andrea Medicinal Chemistry (Neamati)

Completed (66, 32* out of department committees) Aggarwal, Parag Medicinal Chemistry (2006) *Anna, Laura Chemistry (1996) Bell, Jessica Medicinal Chemistry (2012) Birck, M. Medicinal Chemistry (2002) *Blake, Ruth Geological Sciences (1997) Brandvold, Kristoffer Medicinal Chemistry (2014) Breen, Meghan Medicinal Chemistry (2014) Burke (Marques), Fernanda Medicinal Chemistry (2007) *Chen, Bin Chemistry (2005) *Collard, Wendy Pharmaceutics (1999) *Dietz, Amber Chemistry (2005) Dotson, Garry Medicinal Chemistry (1994) Emonds, Mark Medicinal Chemistry (1993)


20

Fowler, Carol Medicinal Chemistry (2003) Gu, Liangcai Medicinal Chemistry (2008) Gudmunsson, Kristjan Medicinal Chemistry (1996) *Harris, Dinari Chemistry (2004) *Hedglin, Mark Chemical Biology (2010) *Heimbach, Tycho Pharmaceutics (2003) Ho, Jeffery Medicinal Chemistry (1996) Holt, Melissa Medicinal Chemistry (2014, MS) *Holub, David Chemistry (1995) *Hopkins, Nancy Biological Chemistry (1994) Hurshman, Amy Medicinal Chemistry (2000) Johnson, Taylor Medicinal Chemistry (2016) Joseph, Georges-son (Caleb) Medicinal Chemistry (2012) Kaplan, Will Medicinal Chemistry (2017) *Karamchand, Leshern Chemical Biology (2014) Khanna, Hemant Medicinal Chemistry (2001) Kwarcinski, Frank Medicinal Chemistry (2015) *Kwok, Kai Pharmaceutics (2001) *Lee, Tien-Yi Pharmaceutics (2011) *Lennon, Brett Biological Chemistry (1995) Li, Shasha Medicinal Chemistry (2017) *Liu, Xin Chemistry (2013) McKenzie, Donald Medicinal Chemistry (2000) Meredith, Tim Medicinal Chemistry (2005) *Nguyen, Hanh Nho Chemistry (2002) Nwokoye, Adaora Medicinal Chemistry (2011) *Ortiz, Mariliz Biological Chemistry (1999) *Pais, June Biological Chemistry (2007) *Pan, Jonathan Cellular and Molecular Biology (2006) Patrone, James Medicinal Chemistry (2010) *Patury, Srikanth Pathology (2011) Perry, Jason Medicinal Chemistry (2000) *Rider, Lance Biological Chemistry (2008) *Rinaldi, Arlie Chemistry (2013) *Rubio, Maria Belen Chemistry (2000)


21

*Savall, Brad Chemistry (2002) *Shin, Meong Cheol Pharmaceutics (2013) *Sirdeshpande, Bhagyashri Chemistry (1996) Smith, April Medicinal Chemistry (2001) Spiering, Michelle Medicinal Chemistry (2002) Sripathi, Kamali Medicinal Chemistry (2013) *Stull, Fredrick Chemical Biology (2014) Sun, Jing Medicinal Chemistry (2010) *Szkudlarek, Beth Pharmaceutics (1997) *TenHoor, Chris Pharmaceutics (1993) Tomsho, John Medicinal Chemistry (2004) Walker, John Medicinal Chemistry (1996) Winger, Jon Medicinal Chemistry (2004) *Wolfe, Abigail Biological Chemistry (2009) Xu, Hao Medicinal Chemistry (2015) Yu, Jing-Yu (Jerry) Medicinal Chemistry (2011) *Zhang, Yaru Chemical Biology (2014) *Zhou, Lily Chemistry (2006)

Pharm. D. INVESTIGATION ADVISOR (14 + 3 in process) Barcomb, Lisa Pharmacy Class of 2002 (Recipient of 2002 PharmD Research Award) Elijah, Joseph Pharmacy Class of 2021 (In Process) Emeigh, Candice Pharmacy Class of 2007 Eshelman, Susan Pharmacy Class of 1998 (Recipient of 1998 PharmD Research Award) Grudzinski, Amy Pharmacy Class of 1996 Idrees, Umbreen Pharmacy Class of 2001 Jamal, Sara Pharmacy Class of 1999 Kontos, Arrin Pharmacy Class of 2021 (In Process) LaFreniere, Keila Pharmacy Class of 2020 (In Process) Lalani, Rebecca Pharmacy Class of 2012 Mamdani, Muhammad Pharmacy Class of 1995 Pi, Judi Pharmacy Class of 1995 Sardelli, Laura Pharmacy Class of 2006 Smith, Amy Pharmacy Class of 1994 Thrasher, Claire Pharmacy Class of 2021 (In Process) Ward (Wiese), Kristen Pharmacy Class of 2014


22

Won, Young-Sam Pharmacy Class of 1994 Yu, Michelle Pharmacy Class of 2008

FACULTY MENTOR University of Michigan

Master’s Independent Research Project Kuo, Yu-Hsuan 2013-14, University of Michigan, Chemical Biology MS student (Note: non-thesis MS program)

Undergraduate Research Project Fath, Joan Summer 2011, University of Michigan-Flint undergraduate Holt, Melissa (Christopher) 2010, University of Michigan undergraduate (Biochemistry) Lofchy, Laren 2015-17, University of Michigan undergraduate (ChemE) Neal, Joslyn 2009-10, University of Michigan undergraduate (CMDB) Sánchez, Lisset 2017-18, University of Michigan undergraduate (MolecBiosci)

Undergraduate Honors Research Project Emanuele, Anthony 2008, University of Michigan undergraduate (Biochemistry) Ugur, Fatima 2012-15, University of Michigan undergraduate (Biochemistry)

Undergraduate Research Opportunity Program Dahlin, Christina 2005-6, University of Michigan undergraduate (Engineering) Kontos, Arrin 2013-15, University of Michigan undergraduate (Chemistry)

Summer Research Opportunity Program Furman, Cheryse Summer 2002, UC Irvine undergraduate

College of Pharmacy Bridge to a PhD Program

Babilonia, Elisa Summer 1999, University of Puerto Rico, Mayaguez, MS student Diaz, Edgar Summer 1999, University of Puerto Rico, Mayaguez, MS student Rodriguez, Carlos Summer 1996, University of Puerto Rico, Mayaguez, MS student

International Exchange Program Crochemar-Galou, Agnes Summer 2010, Paris Descartes University, Pharmacy student Tam, Hoi Wan (Irene) Fall 2009, University of Bath, England, Pharmacy student

REU Program Arredondo-Guerrero, Justin Summer 2014, University of the Incarnate Word Kane, Gordon Summer 2013, Beloit College Liyayi, Ian Summer 2017, Stevenson University, Maryland Redwood, Melissa Summer 2009, Eastern Michigan University Sánchez, Lisset Summer 2016, Miami-Dade Community College


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Velez-Martinez, Carol Summer 2010, University of Puerto Rico-Rio Piedras MICHR Summer Research Internship

Thrasher, Claire Summer 2018, Pharmacy Class of 2021 External

Kalamazoo College, Senior Individualized Project Baran, Brenda Summer 2007, Kalamazoo College undergraduate Bauer, Shannon Summer 2005, Kalamazoo College undergraduate Cutcher, Julie Summer 2003, Kalamazoo College undergraduate Hurt, Blake Summer 2004, Kalamazoo College undergraduate

Documents

(updated June 3, 2019) CURRICULUM VITAE GEORGE A. GARCIA ... · CURRICULUM VITAE GEORGE A. GARCIA, Ph. D. Professor and Chair of Medicinal Chemistry ... Committee on Institutional