Document Title : FORMAT FOR CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER

Reference SOP : CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER Reference SOP No : H/SOP/QCD/048/00 Format no. : H/F/QCD/021/00Annexure No. : 01 Page No. : 1 of 4

CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER

Instrument make : Shimadzu Date of calibration :

Model No. : UV 1800 Next Due :

Instrument code No. : QC/UVS/001

Frequency : Quarterly After Maintenance After relocation

1. CONTROL OF WAVELENGTH:

Standard : Holmium Filter Filter No. : _____________

Maxima at wavelength

(Standard)

Maxima at wavelength

(Observed)

Tolerance

nmRemarks

241.15 nm 240.15 to 242.15

287.15 nm 286.15 to288.15

361.50 nm 360.50 to 362.50

536.30 nm 533.30 to 539.30

2. LIMIT OF STRAY LIGHT:

Potassium chloride B.No.: _______________________Make:__________________

Potassium chloride

Gross weight in gm.

Tare weightin gm.

Net weightin gm.

Volume with distilled water

ml.

Wavelength Absorbance Limit Remark

200nm Not less than 2.0Complies/Does not

comply

Prepared by: Approved by:Authorised by:

Date: Date: Date:

Document Title : FORMAT OF CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER

Reference SOP : CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER Reference SOP No : H/SOP/QCD/048/00 Format no. : H/F/QCD/021/00Annexure No. : 01 Page No. : 2 of 4

3. CONTROL OF ABSORBANCE

Potassium dichromate:

B.No. Make Dried On Next due

Potassium dichromate

Gross weight in gm.

Tare weightin gm.

Net weightin gm.

Volume of 0.005M H2SO4. required (ml)

ml.(Solution- A)

Further dilution: ____________ml/_________ml. in 0.005M H2SO4.(Solution-B)

WavelengthObserved

Absorbance

Actual

Extinction(E)

Observed

Extinction(E)

Maximum

ToleranceRemark

235nm 124.5 122.90 to 126.20

Complies/Does not comply

257nm 144.5 142.80 to 146.20

Complies/Does not comply

313nm 48.6 47.00 to 50.30

Complies/Does not comply

350nm 107.3 105.6 to 109.0

Complies/Does not comply

430nm 15.9 15.7 to 16.1

Complies/Does not comply

Calculation for actual Extinction:

Observed Absorbance Xdilution factor Wt. of Pot. Dichromate taken X 100

Prepared by: Approved by:Authorised by:

Date: Date: Date:

Document Title : FORMAT OF CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER

Reference SOP : CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER Reference SOP No : H/SOP/QCD/048/00 Format no. : H/F/QCD/021/00Annexure No. : 01 Page No. : 3 of 4 4. RESOLUTION POWER

Toluene B.No.: Make :

Dilution: _______ml of toluene in _______ml of hexane.

Absorbance at maximum at about 269 nm (A)

Absorbance at minimum at about 266 nm (B)

Ratio =A/B

Limit : Less than 1.5

Remark: Complies / Does not complies

Note: All wavelength scan are enclosed.

Conclusion:

From the above observations we can conclude that Instrument is Complies / Does not comply above

checks and is suitable for routine work.

Compiled By: Checked By: Approved By:

Date: Date: Date :

Prepared by: Approved by:Authorised by:

Date: Date: Date:

Document Title : FORMAT OF CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER

Reference SOP : CALIBRATION OF UV/VISIBLE SPECTROPHOTOMETER Reference SOP No : H/SOP/QCD/048/00 Format no. : H/F/QCD/021/00Annexure No. : 01 Page No. : 4 of 4

1. CONTROL OF WAVELENGTH: 3. CONTROL OF ABSORBANCE:

2. LIMIT OF STRAY LIGHT: 4. RESOLUTION POWER:

Prepared by: Approved by:Authorised by:

Date: Date: Date:

Analyst Sign / Date