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Varian 900-LC Series iHPLC
2
What is the iHPLC?
• 2 models 920-LC and 940-LC
• Compact, stylish, automated analytical and semi prep HPLC
• Pre-plumbed and factory tested for the highest confidence
• Easy to order, install and use
– Single Ethernet cable connection
• Focused on improved productivity through automation
• Updated premium performance hardware
• Autosampler, Fraction Collector and Scale up Module for semi-prep
• Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical
• Updated Integrated Instrument Software Interface
• Eliminate repetition, modernise UI
• Consistent
• Easy to use with added functionality
• Adds to modular range
3
Varian 920-LC Integrated Analytical HPLC
4
Varian 920-LC What Is It?
• Compact, stylish automated analytical HPLC
– Pre-plumbed and factory tested for the highest confidence
– Single cable communications
• Virtually pulse-free dual piston pump
– Does not require a pulse damper
• State-of-the-art low pressure quaternary gradient module with built-in degasser
• Autosampler with cooling option
– Synchronised injection for outstanding retention time reproducibility
– Compatible with 200 x 1.6 ml HPLC vials or 3 microplates
5
Varian 920-LC What Is It?
• Either dual UV-Vis or PDA detector
– Optional second detector - Fluorescence or RI
• High precision (<0.15oC) dual column heater available
– Low delay solvent pre-heater for improved heat transfer
– Optional column switching and electric injection valve
• NEW improved instrument interface on GalaxieTM software
• External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.
6
Varian 920-LC Silver Bullets
• Premium analytical pump performance and reliability
• Superb Fast LC performance optimised for sub 3 um columns
– Improved throughput without compromising robustness
• High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength
• Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler
• Full system level computer controlled AIQ (validation)
• Updated driver for enhanced user experience
7
920-LC from Conventional to Fast HPLC
25cm x 4.6mm Pursuit XRS 5um 1.0ml/min
25cm x 4.6mm Pursuit XRS 5um 2.2ml/min
15cm x 4.6mm Pursuit XRS 5um 1.0ml/min
15cm x 4.6mm Pursuit XRS 5um 3.75ml/min
10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min
5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min
5cm x 2.0mm 2.4um 1.3ml/min16 x Speed Enhancement
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Varian 920-LC Fast LC PAHs
Varian 920-LC6 minute Analysis
Waters Acquity7 Minute Analysis
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Varian 920-LC Solvent Delivery System
• Dual piston quaternary pump produces fast gradients – even at 200 l/min
• Virtually pulse-free pumping without pulse damper
• Outstanding gradient compositional accuracy give retention time precision at the <1% level
• Built-in vacuum degas maintains pump prime and enhanced injection reliability
• Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns
• Overlay of 10 chromatograms
• 10% steps at 1ml/min
10
Varian 920-LC Autosampler
• Needle-in-flow path geometry for low carry-over (<0.01%)
• Typical inj precision (< 0.3% at 10 l)
• Optimized flow paths for fast injection turn-around
• Synchronised injection for best RT reproducibility
• Programmable wash setting for low carry-over
• Injection volumes from 1ul to 50L
• Cooled option available
11
Varian 920-LC Washout Performance
Blank injection <0.01% carryover
125 ug/ml Caffeine
12
Varian 920-LC Injection Synchronization
-20%
0%
20%
40%
60%
80%
100%
120%
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2
13
Sensitive UV-Vis Detectors
Typical Agilent VWD S/N ~ 25
Typical Varian 920-LC UV/Vis S/N > 36
14
Information Rich Diode Array Detection
• 1024 pixels provides full coverage from 190 – 950 nm
• Full spectra collection• Enhanced purity
• Automatic peak identification with customised libraries• Greater confidence on compound identification
• Choice of flowcells• 4 mm path length semi-micro • 9mm path length analytical
• Front panel access• Lamps and flowcells
• D2 and Vis lamps self aligned• Easily user maintained
15
Information Rich Diode Array Detection
• Automatic peak identification with customised libraries• Use spectra as well as RT for compound ID
• Greater confidence on compound identification
• Peak search of unknown yields 998.7 match for naphthalene
16
Varian 920-LC Fluorescence Detector
• Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)
• Time programmable for increase sensitivity• Automatic validation with built-in Hg lamp• Low 12 l dead volume flowcell for improved
chromatography
17
Varian 920-LC Refractive Index Detector
• Based upon 356-LC RI detector• Low volume, flow efficient cell for superior peak shape• Stable, long life and high intensity LED
• Minimises drift
• Provides excellent detection limits
• Enhanced life-time
• Temperature controlled optics minimises baseline drift and provides outstanding detection limits
• Complete automation thru Galaxie software
18
Varian 380-LC and 385-LC ELSD
• Universal HPLC detector• Compatible with gradients
• More sensitive than RI
• NEW Glass Nebuliser for Improved Reproducibility• 2 to 3 Times More Sensitive
• 40Hz Data Rate for Fast LC• Complete Galaxie compatibility
• Program changes thru a run
19
Heated Column Compartment
• Temperature range from ambient + 10oC up to 65oC
• Excellent temperature precision +0.15oC
– Ensures retention time reproducibility
– Eliminates re-runs
• Good temperature accuracy <1oC
– Low delay volume pre-heater for efficient heat transfer to mobile phase
• Rapid warm-up
– Typically from ambient to 40oc in <15 minutes
• Spacious - easily accommodates 2 x 250mm columns and 2 valves
• Option with electrically actuated column switching valve and injection valve
20
Excellent Long term Stability
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
11:4
6:00
12:2
2:00
12:5
8:00
13:3
4:00
14:1
0:00
14:4
6:00
15:2
2:00
15:5
8:00
16:3
4:00
17:1
0:00
17:4
6:00
18:2
2:00
18:5
8:00
19:3
4:00
20:1
0:00
20:4
6:00
21:2
2:00
21:5
8:00
22:3
4:00
23:1
0:00
23:4
6:00
0:22
:00
0:58
:00
1:34
:00
2:10
:00
2:46
:00
3:22
:00
3:58
:00
4:34
:00
5:10
:00
5:46
:00
6:22
:00
6:58
:00
7:34
:00
8:10
:00
8:46
:00
9:22
:00
Time
Te
mp
era
ture
(d
eg
ree
s C
)
Mobile phase input Temp
Mobile phase temp inside column
Oven Temp
21
Quick Warmup - Outstanding Stability
20
22
24
26
28
30
32
34
36
38
40
42
44
8:00
:00
8:10
:00
8:20
:00
8:30
:00
8:40
:00
8:50
:00
9:00
:00
9:10
:00
9:20
:00
9:30
:00
9:40
:00
Time
Te
mp
era
ture
(d
eg
ree
s C
)
Ambient Room
Column heater
Column
22
Reproducibility of 50 Injections
Reproducibility <0.8% over 50 injections and 12 hours
23
Varian 920-LC Software Usability
• User friendly GalaxieTM software
• Simplified, modern, consistent Instrument interface
• Modular 11 pages
• 920-LC 4 pages!!
• One page real-time status and chromatogram
• Advanced integration and calibration
• New IMS capabilities
• Automated instrument validation
• Reduces downtime and operating costs
• Fusion AE
24
Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC• Leak detector
• Increases confidence with unattended automated runs
• Single waste point• Ensures capture of all wastes
• Easy to connect to waste vessel
• Built-in Solvent bottle storage• Improved safety and convenience
• Lower hight than modular systems
25
Varian 920-LC Integrated HPLC
Special features of the Varian 920-LC• Clear front doors
• Stylish
• Provides access to autosampler without opening
• Safety increased as lab staff shielded from solvent spray and leaks
• Useful, convenient storage compartments• Column rack in door
• Tool drawer
• Operation log drawer
26
Varian 920-LC AIQ (Validation)
AIQ (Analytical Instrument Qualification)• Fully PC controlled IQ and OQ (not PQ)
• Puts the Varian 920-LC at the forefront of regulated capabilities
• Consistent, reproducible methodology• Includes pumps, autosampler, detectors, column
heater
• Chargeable option
• Fully documented
27
900 LC -Series AIQ
• Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package.
• Verifies the entire LC system by systematically running the following
• Flow Rate Accuracy Test
• Wavelength Detector Accuracy Test
• Auto-sampler Injector Accuracy Test (also measures Detector Linearity)
• Auto-sampler Injector Precision Test
• Auto-sampler Injector Carryover Test
• Proportioning Accuracy Test
• Detector Noise Test
• Detector Drift Test
• Auto-sampler Vial Identification Accuracy Test
• Proportioning Linearity
• Compositional Ripple
28
Varian 920-LC Configurations
pump inject detector Oven Detector 2
option
Options
Isocratic
00-100890-00Dual piston pump 10 ml head
Auto Dual UV/Vis
Opt FL, RI ELSD
Gradient
00-100891-00Quat, Grad, Degas Auto Dual
UV/VisOpt FL, RI ELSD opt
High Performance Gradient
00-100892-00
Quat, Grad, Degas Auto cooled Dual UV/Vis
Opt FL, RI ELSD opt
Development
00-100893-00Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt
Research
00-100894-00Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt
29
Varian 920-LC Consumables
• Excellent range of HPLC Columns providing industry leading resolution and robustness
• Pursuit
• Pursuit XRS
• Polymeric Phases - PL
• From 2mm to 2” ID Prepacked
• Other Speciality Phases
• NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC
• 2.8um Ultra is optimised for Speed
• 2.4um gives further enhancements in Resolution
30
2.8um Vs 2.4 um – High Res or Extra Speed
2.8um 2mm x 5cm for speed
2.4um 3mm x 5cm for RESOLUTION
Questions??