Metabolism · various metabolism Assay Kits: A Quick glance Tech help: email: [email protected] or call 800.891.9699 or 650-428-0236 or fax: 650.428.0336 C ColorimetricF L Assay

MetabolismAssay Kits

FINIAL-11-6-09-brochure.indd 1 11/16/2009 2:34:11 PM

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order instructions & warranty information

� DatePrinted:November,2009


Metabolism refers to the catabolic & anabolic biochemical reactions inside of the cell. It is the process (or really the sum of manyongoingenzymereactions)bywhichlivingcellsprocessnutrientmoleculesandmaintainalivingstate.Metabolisminvolvescomplexsequences of controlled chemical reactions called metabolic pathways. The diagram below shows some of the important metabolicpathwaysthatarecommonlystudiedbyresearchers.OurscientistsatBioVisionhavedevelopedanextensivelineofassaykitsusingourproprietaryprobesandtechnologies.Theassaysaredesignedtobeperformedina96-wellplateformatandanalyzedbyfluorometricand/orcolorimetricmethods.Thesemetabolicassaysaresensitive,accurateandeasytoperform.Moreover,thekitsaresuitableforuseinvariousindustriesandlaboratories,includingthoseinvolvedindrugdiscovery,preclinicalandclinicalresearch,nutrition,aswellas,thefoodandwineindustry.

�ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Metabolism Overview

features & Benefits

•Straight-forwardprocedure•Easytoperform,justadd-mix-read•Sensitive,accurateandreliable•Wideassayrange(2-200µM)•Easytocalculateresults•Bothfluorometricandcolorimetricmethodsareavailable•Suitableforhigh-throughputassays

general Assay Diagram

Sample Preparation

Add Reaction Mix

Read Fluorescence or Absorbance

Incubate


various metabolism Assay Kits:A Quick glance

� Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336

C F LColorimetricAssayKit C F LFluorometricAssayKit C F L BioluminescenceAssayKit

Amino Acids & ProteinsProduct Type Cat.# Size Page

AlanineAssayKit CF K652-100 100assays 8

L-AminoAcidQuantificationKit CF K639-100 100assays 9

BranchedChainAminoAcidAssayKit C K564-100 100assays 12

GlutamateAssayKit C K629-100 100assays 20

PhenylalanineAssayKit F K572-100 100assays 33

UreaAssayKit CF K375-100 100assays 38

Carbohydrate MetabolismProduct Type Cat.# Size Page

AmylaseAssayKit C K711-100 100assays 10

FreeGlycerolAssayKit CF K630-100 100assays 17

FructoseAssayKit CF K619-100 100assays 18

GalactoseAssayKit CF K621-100 100assays 18

GlucoseAssayKit CF K606-100 100assays 19

GlycogenAssayKit CF K646-100 100assays 22

LactoseAssayKit CF K624-100 100assays 28

MaltoseandGlucoseAssayKit CF K618-100 100assays 30

MaltoseAssayKit CF K628-100 100assays 30

SialicAcid(NANA)AssayKit CF K566-100 100assays 36

StarchAssayKit CF K647-100 100assays 36

SucroseAssayKit CF K626-100 100assays 37

Coenzymes & CofactorsProduct Type Cat.# Size Page

PicoProbeTMAcetyl-CoAAssayKit F K317-100 100assays 6

ADPAssayKit CF K355-100 100assays 8

ATPAssayKit CF K354-100 100assays 11

ATPCellViabilityAssayKit L K254-200 200assays 11

ADP/ATPRatioAssayKit L K255-200 200assays 8

CoenzymeA(CoA)AssayKit CF K367-100 100assays 15

FADAssayKit CF K357-100 100assays 17

GlutathioneColorimetricAssayKit C K261-100 100assays 21

GlutathioneFluorometricAssayKit F K251-100 100assays 21

Glutathione(GSH/GSSG/Total)AssayKit F K264-100 100assays 20

GlutathionePeroxidaseAssayKit C K762-100 100assays 21

GlutathioneReductaseAssayKit C K761-200 200assays 22

HemeAssayKit CF K672-100 100assays 25

NAD/NADHQuantificationKit C K337-100 100assays 31

NADP/NADPHQuantificationKit C K347-100 100assays 31

Inorganic IonsProduct Type Cat.# Size Page

AmmoniaAssayKit CF K370-100 100assays 10

CalciumAssayKit C K380-250 250assays 12

ChlorideAssayKit C K530-100 100assays 14

CobaltAssayKit C K505-100 100assays 15

IronAssayKit C K390-100 100assays 26

MagnesiumAssayKit C K385-100 100assays 29

NickelAssayKit C K510-100 100assays 31

NitricOxideColorimetricAssayKit C K262-200 2x96assays 32

NitricOxideFluorometricAssayKit F K252-200 2x96assays 32

PhosphateColorimetricAssayKit C K410-500 500assays 33

PhosphateFluorometricAssayKit F K420-100 100assays 33

Lipid MetabolismProduct Type Cat.# Size Page

AdipogenesisAssayKit CF K610-100 100assays 7

AdipolysisAssayKit CF K611-100 100assays 7

CETPActivityAssayKit F K601-100 100assays 13

CETPInhibitorDrugScreeningKit F K602-100 100assays 13

CholesterolQuantificationKit CF K603-100 100assays 14

FreeFattyAcidQuantificationKit CF K612-100 100assays 17


HDLandLDL/VLDLQuantificationKit CF K613-100 100assays 24

b-Hydroxybutyrate(b-HB)AssayKit C K632-100 100assays 25

LipaseAssayKit CF K722-100 100assays 29

PhosphatidylcholineAssayKit CF K576-100 100assays 34

PLTPActivityAssayKit F K604-100 100assays 34

PLTPInhibitorDrugScreeningKit F K605-100 100assays 34

TriglycerideQuantificationKit CF K622-100 100assays 38


Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 �

Intermediary MetabolismProduct Type Cat.# Size Page

PicoProbeTMAcetyl-CoAAssayKit F K317-100 100assays 6

AconitaseAssayKit C K716-100 100assays 7

AmylaseAssayKit C K711-100 100assays 10

L-CarnitineAssayKit CF K642-100 100assays 13

CitrateAssayKit CF K655-100 100assays 15

Choline/AcetylcholineQuantificationKit CF K615-100 100assays 14

CoenzymeA(CoA)AssayKit CF K367-100 100assays 15


FructoseAssayKit CF K619-100 100assays 18

GlucoseandSucroseAssayKit CF K616-100 100assays 19


Glucose-6-PhosphateAssayKit C K657-100 100assays 19

Glucose-6-PhosphateDehydrogenaseAssayKit C K757-100 100assays 20




IsocitrateAssayKit C K656-100 100assays 26

a-KetoglutarateAssayKit CF K677-100 100assays 26

D-LactateColorimetricAssayKit C K667-100 100assays 27

L-LactateAssayKit CF K607-100 100assays 27

L-LactateAssayKitII C K627-100 100assays 27

LDHQuantificationKit C K726-500 500assays 28

LDH-CytotoxicityAssayKit C K311-400 400assays 28

LDH-CytotoxicityAssayKitII C K313-500 500assays 29

LipaseAssayKit CF K722-100 100assays 29

MalateAssayKit C K637-100 100assays 30

NAD/NADHQuantificationKit C K337-100 100assays 31

NADP/NADPHQuantificationKit C K347-100 100assays 31

OxaloacetateAssayKit CF K659-100 100assays 32

PyruvateAssayKit CF K609-100 100assays 35

PyruvateKinaseAssayKit CF K709-100 100assays 35

SarcosineAssayKit CF K636-100 100assays 35

Oxidative StressProduct Type Cat.# Size Page

AscorbicAcidAssayKit CF K661-100 100assays 10

AscorbicAcidAssayKitII(FRASC) C K671-100 100assays 11

CatalaseAssayKit CF K773-100 100assays 12

EthanolAssayKit CF K620-100 100assays 16




GlutathionePeroxidaseAssayKit C K762-100 100assays 21

GlutathioneReductaseAssayKit C K761-200 200assays 22

GSTColorimetricAssayKit C K263-100 100assays 22

GSTFluorometricAssayKit F K260-100 100assays 23

HDACColorimetricAssayKit C K331-100 100assays 23

HDACFluorometricAssayKit F K330-100 100assays 24

HDACInhibitorDrugScreeningKit F K340-100 100assays 24

HATActivityAssayKit C K332-100 100assays 23

HydrogenPeroxideAssayKit CF K265-200 200assays 25

NitricOxideColorimetricAssayKit C K262-200 2x96assays 32

NitricOxideFluorometricAssayKit F K252-200 2x96assays 32

SODActivityAssayKit C K335-100 100assays 36

ThioredoxinReductaseAssayKit C K763-100 100assays 37

TotalAntioxidantCapacityAssayKit C K274-100 100assays 37

UricAcidAssayKit CF K608-100 100assays 38

XanthineOxidaseAssayKit CF K710-100 100assays 39

Serum ComponentsProduct Type Cat.# Size Page

AcidPhosphataseColorimetricAssayKit C K411-500 500assays 6

AcidPhosphataseFluorometricAssayKit F K421-500 500assays 6

AlkalinePhosphataseColorimetricAssayKit C K412-500 500assays 9

AlkalinePhosphataseFluorometricAssayKit F K422-500 500assays 9

CholesterolQuantificationKit CF K603-100 100assays 14

CreatineAssayKit CF K635-100 100assays 16

CreatinineAssayKit CF K625-100 100assays 16


Glucose-6-PhosphateAssayKit C K657-100 100assays 19

HDLandLDL/VLDLQuantificationKit CF K613-100 100assays 24

LactateAssayKit CF K607-100 100assays 27

LactateAssayKitII C K627-100 100assays 27

TriglycerideQuantificationKit CF K622-100 100assays 38


� ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Pico ProbeTMAcetyl CoA Quantification Kit

PicoProbeTMAcetylCoAQuantificationKit Cat.#K317-100 100assays

AcidPhosphataseColorimetricAssayKit Cat.#K411-500 500assays

Acetyl-CoA (AcCoA) is the metabolic intermediate which transfers carbon atoms tothe TCA cycle for energy production and is the link between fat and carbohydratemetabolism. When fatty acid levels are high, AcCoA levels rise beyond the energyrequirementsoftheorganismandtheexcessisconvertedtotheketonebodiesacetoneand β-hydroxybutyrate. AcCoA also contributes Acetate for histone acetylation andisoprenoid synthesis. BioVision has developed an easy, convenient assay to measurethe AcCoA level in biological samples. In the assay, AcCoA is specifically utilized togenerate products which react with OxiRed™Probe to generate color (λmax= 570 nm)andfluorescence(Ex/Em=535/587nm).Theassaycandetect0.1to10nmolofAcCoAinavarietyofsamples.

Acidphosphatases(AP)dephosphorylatephosphateestersunderacidconditions.Differentacidphosphataseisozymesarefoundindifferentorgansandtheirserumlevelsareusedas a diagnostic for disease in the corresponding organs. BioVision’s Acid PhosphataseAssay Kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assayfor measuring AP activity in serum and other samples. The kit uses p-nitrophenylphosphate(pNPP)asaphosphatasesubstratewhichturnsyellow(λmax=405nm)whendephosphorylatedbyAP.ThekitcandetectμUacidphosphataseactivityinsamples.

y = 57.006x + 0.1088R² = 0.9988

0

0.5

1

1.5

0 0.005 0.01 0.015 0.02 0.025

O.D.405nm

pNP (µmol)

pNP Standard Curve

y = 2.3831x + 0.0027R² = 0.9999

0

0.2

0.4

0.6

0.8

1

1.2

0 0.1 0.2 0.3 0.4 0.5

O.D.405

nm

AP (mU)

AP Enzyme Samples 40min Assay

y = 57.006x + 0.1088R² = 0.9988

0

0.5

1

1.5

0 0.005 0.01 0.015 0.02 0.025

O.D.405nm

pNP (µmol)

pNP Standard Curve

y = 2.3831x + 0.0027R² = 0.9999

0

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0 0.1 0.2 0.3 0.4 0.5

O.D.405

nm

AP (mU)

AP Enzyme Samples 40min Assay

Kit Contains: AP Assay Buffer, pNPP Substrate, AP Enzyme, Stop Solution and anInstructionManual.

y = 0.0906x + 0.0103

0

0.2

0.4

0.6

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1

0 2 4 6 8 10

OD(570nm)

nmol Acetyl CoA

y = 5349.1x + 305.19

0

2000

4000

6000

8000

10000

0 0.4 0.8 1.2 1.6 2

RFU

nmol Acetyl CoA

Kit Contains:AcCoAAssayBuffer,OxiRed™Probe,DMSO,ConversionEnzymeMix,AcCoASubstrate,AcCoADeveloper,AcCoAStandardandanInstructionManual.

Acid Phosphatase Colorimetric Assay Kit

Acid Phosphatase Fluorometric Assay Kit

AcidPhosphataseFluorometricAssayKit Cat.#K421-500 500assays

Acid phosphatase (AP) dephosphorylates phosphate esters in acid conditions. Differentforms of acid phosphatase are found in different organs and their serum levels are usedas a diagnosis for disease in the corresponding organs. For example, elevated prostaticacid phosphatase levels may indicate the presence of prostate cancer. BioVision’s AcidPhosphatase Fluorometric Assay Kit uses non-fluorescent Methylumbelliferyl phosphatedisodium(MUP)asthesubstratewhichhasEx/Em=360/440nmwhendephosphorylatedbyAP.Thekitprovidesanultra-sensitive,simple,directandHTS-readyfluorometricassaytomeasureAPactivityinserumandotherbiosamples.Thedetectionsensitivityis~1μU,moresensitivethancolorimetricassays.

Kit Contains: AP Assay Buffer, MUP Substrate, AP Enzyme, Stop Solution and anInstructionManual.

y = 19114x + 593.43R² = 0.9993

0

5000

10000

0 0.1 0.2 0.3 0.4 0.5

RFU

4 MU(nmol)

AP 4 MU Standard Curve

C F L

C F L

C F L



Aconitase Assay Kit

AconitaseAssayKit Cat.#K716-100 100assays

Aconitaseisaniron-sulfurproteincontaininga[Fe4S4]2+clusterthatcatalyzesthestereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acidcycle.Intissueextractsthereareactuallytwoaconitases,amitochondrial(m-)aconitaseandacytosolic(c-)aconitase.Theyarerelated,butdistinctlydifferentenzymesandarecodedondifferentchromosomes.Lossofaconitaseactivityincellsorinbiologicalsamplestreated with pro-oxidants has been interpreted as a measure of oxidative damage.BioVision’sAconitaseAssayKitprovidesahighlysensitive,simple,directandHTS-readycolorimetric assay for measuring Aconitase activity in biological samples. In the assay,citrate is converted by aconitase into isocitrate and then the Isocitrate converted byisocitratedehydrogenasetoα-ketoglutarateinthepresenceofNAD+/NADP+.TheincreaseofNADH/NADPHcanbedetectedbydeveloperandcanbemeasuredatOD=450nm.

y = 0.0625x + 0.021R² = 0.9985

0

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1

1.2

0 10 20

O.D. 450

nm

L Isocitrate (nmol)

Isocitrate Standard

Kit Contains: Aconitase Assay Buffer, Enzyme Mix, Aconitase Substrate, Aconitase Developer,Isocitrate Standard, Aconitase Positive Control, Aconitase Activation Mix and an InstructionManual.

Obesity is a significant risk factor in a variety of disease states including diabetes,atherosclerosis, cardiovascular disease and cancer. Adipogenesis is the process wherebyadiposetissueisformedthroughthedifferentiationofseveralcelltypesintoadipocytes.Adipocytes can efficiently accumulate lipids in the form of lipid droplets. BioVision’sadipogenesiskitquantifiestheamountoftriglycerideaccumulatedincells,whichcanbeusedasameasureoftheeffectsofvariousinducersandinhibitorsinthepathwaysleadingtolipidaccumulation.

Kit Contains:AssayBuffers,EnzymesandothercomponentsnecessarytodetecttriglyceridesinadipocytesincellcultureandanInstructionManual.

Adipogenesis Assay Kit

AdipogenesisAssayKit Cat.#K610-100 100assays

Adipolysis Assay Kit

AdipolysisAssayKit Cat.#K611-100 100assays

Adipolysis is the highly regulated process wherein triglycerides are broken downinto their component parts, free fatty acids and glycerol. Accurate measurement ofadipolysisisveryusefulfordeterminingpathwaysandtargetmoleculesinvolvedinavarietyoflipidstorageandutilizationpathways.BioVision’sAdipolysisKitisdesignedtoprovideasimpleaccuratemethodofquantifyingtheamountofglycerolgeneratedduringadipolysisincells.

Kit Contains: Assay Buffers, Enzymes and other components necessary for the accuratequantificationofglycerolincellsundergoinglipidhydrolysisandanInstructionManual. 0

0.4

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0 2 4 6 8 10

OD57

0nm

Glycerol (nmol/well)

0

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0 2 4 6 8 10

OD570nm

Triglyceride (nmol/well)

C F L

C F L

C F L


� ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

ADP Colorimetric and Fluorometric Assay Kit

ADP/ATP Ratio Assay Kit

ADPColorimetricandFluorometricAssayKit Cat.#K355-100 100assays

ADP/ATPRatioAssayKit Cat.#K255-200 200assays

ADP is a product of ATP dephosphorylation and can be rephosphorylated to ATP. ADPlevels regulate several enzymes involved in intermediary metabolism. Conventionally,ADP levels are measured by luciferase/luciferin mediated assays after ADP is convertedto ATP. However, the luciferase system is unstable and luminescence equipment is notgenerally available in most laboratories. BioVision’s newly designed ADP Assay Kitprovides a convenient colorimetric and fluorometric means for measuring ADP. In theassay,ADPisconvertedtoATPandpyruvate.Thegeneratedpyruvatecanbequantifiedbycolorimetric(λmax=570nm)orfluorometric(Ex/Em=535/587nm)methods.Theassayissimple,sensitive,stableandhigh-throughputadaptable.Theassaycandetectaslowas1 mMADPinbiologicalsamples.

The changes in ADP/ATP ratio have been used to differentiate the different modes ofcell death and viability. Increased levels of ATP and decreased levels of ADP have beenrecognizedinproliferatingcells.Incontrast,decreasedlevelsofATPandincreasedlevelsofADParerecognizedinapoptoticandnecroticcells.TheADP/ATPRatioAssaykitutilizesbioluminescent detection of the ADP and ATP levels. The assay utilizes the enzymeluciferasetocatalyzetheformationof lightfromATPandluciferinandthelightcanbemeasuredusingaluminometerorBetaCounter.ADPlevelismeasuredbyitsconversionto ATP that is subsequently detected using the same reaction. The assay can be fullyautomatedforhigh-throughputpurposesandishighlysensitive(detects100mammaliancells/well).

y = 0.123x 0.0281

0

0.5

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0 2 4 6 8 10

OD570nm

ADP (nmol)

Kit Contains: Nucleotide Releasing Buffer, ATP Monitoring Enzyme, ADP ConvertingEnzyme,EnzymeReconstitutionBufferandanInstructionManual.

Kit Contains:ADPAssayBuffer,ADPProbe,DMSO,ADPConverter,ADPDeveloperMix,ADPStandardandanInstructionManual.

Alanine Assay Kit

AlanineAssayKit Cat.#K652-100 100assays

Alanine is the second most abundant of the 20 proteinogenic amino acids. Whilenonessential, it plays a key role in the glucose-alanine cycle between tissues and liver.Thereappearstobeacorrelationbetweenalaninelevelsandhighbloodpressure,energyintake,cholesterollevelsandbodymassindex.BioVision’sAlanineAssayKitprovidesaneasyandsensitivedetectionofalanineinvarioussamples.Inthekit,alanineisconvertedtopyruvatewhichisthendetectedspecificallybycolorimetric(λmax=570nm;detectingrange0.5-10nmol)orfluorometric(Ex/Em=535/587nm;detectingrange0.05-1nmol)methods.Thekitcandetect~2μMconcentrationofalanineinvarioussamples.

y = 7142.6x 150.29

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RFU

535/587nm

x10

3

L Alanine (nmol)y = 0.1232x 0.0203

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OD570n

m

L Alanine (nmol)

Kit Contains:AlanineAssayBuffer,Alanineprobe,DMSO,AlanineConvertingEnzyme,AlanineDevelopmentMix,AlanineStandardandanInstructionManual.

C F L

C F L

C F L



Alkaline Phosphatase Colorimetric Assay Kit

Alkaline Phosphatase Fluorometric Assay Kit

AlkalinePhosphataseColorimetricAssayKit Cat.#K412-500 500assays

AlkalinePhosphataseFluorometricAssayKit Cat.#K422-500 500assays

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkalinebuffer and produces an organic radical and inorganic phosphate. Changes in alkalinephosphatase level and activity are associated with various disease states in the liverandbone.BioVision’sAlkalinePhosphataseAssayKitprovidesahighlysensitive,simple,directandHTS-readycolorimetricassaytomeasureALPactivityinserumandbiologicalsamples. The kit contains 10 substrate tablets providing convenience for multipleexperiments. The kit usesp-nitrophenyl phosphate (pNPP) as a phosphatase substratewhich turns yellow (λmax= 405 nm) when dephosphorylated by ALP. The Kit can detect10-250μUALPinsamples.

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkalinebufferandproducesanorganicradicalandinorganicphosphate.Thechangeinalkalinephosphataselevelandactivityisassociatedwithavariousdiseasesintheliverandbones.IntheAlkalinePhosphataseFluorometricAssay,ALPcleavesthephosphategroupofthenon-fluorescent4-Methylumbelliferylphosphatedisodiumsalt(MUP)substrateresultinginanintensefluorescentsignal(Ex/Em=360/440nm).Thekitprovidesanultrasensitive,simple,directandHTS-readyassaytomeasureALPactivityinserumandbiosampleswithdetectionsensitivity~1μU,moresensitivethancolorimetricassays.

y = 23706x + 548.05R² = 0.998

0

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RFU

4 MU (nmol)

ALP 4 MU standard curve

y = 56.607x + 0.0741R² = 0.9982

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O.D.4

05nm

pNP (µmol)

pNP Standard Curve

Kit Contains: ALP Assay Buffer, pNPP, ALP Enzyme, Stop Solution and an InstructionManual.

Kit Includes: ALP Assay Buffer, MUP Substrate, ALP Enzyme, Stop Solution and anInstructionManual.

L-Amino Acid Quantification Kit

L-AminoAcidQuantificationKit Cat.#K639-100 100assays

L-Amino acids are the most essential elements in biology. Accurately quantitatingL-amino acids in body fluids or purified samples may provide valuable informationfor diagnostic or basic research studies. BioVision’s L-Amino Acid Assay Kit providesaconvenientmeans fordirectlydetectingL-aminoacids inbiological samples.Thereis no requirement for sample pretreatment or purification when using this kit. TheL-amino acid(s) level can be quantified using fluorometric (Ex/Em = 535/587 nm) orcolorimetric(λmax=570nm)methodsin96-wellplates.

Kit Contains: ALP Assay Buffer, pNPP, ALP Enzyme, Stop Solution and an InstructionManual.

0 6

1.2

1.8

. 570

nm

0

0.6

0 8 16 24 32 40

O.D

.

Total L-Amino Acid (nmol/Well)

C F L

C F L

C F L


10 ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Ammonia Assay Kit

AmmoniaAssayKit Cat.#K638-100 100assays

y = 0.0912x + 0.1426R² = 0.99850

0.5

1

0 5 10

O.D.570nm

NH4+ (nmol)

Ammonium standard curve

Ammonia is an important source of nitrogen for living systems. Nitrogen is requiredfor the synthesis of amino acids, which are the building blocks of protein. Ammonia isa metabolic product which is created through normal amino acid deamination. It alsoplaysanimportantrole inanimalphysiologysuchasnormalanimalacid/basebalance.BioVisionprovidesarapid,simple,sensitiveandreliableassayforbothresearchandhigh-throughput assay of Ammonia in samples. In the assay, Ammonia reacts as substratewith compounds in the presence of enzymes to form a product that reacts with theOxiRed™probetogeneratecolor(λmax=570nm)whichcanbeeasilyquantifiedbyplatereader.Thedetectionlimitis1nmol(~20 mM).

Kit Contains: Ammonia Assay Buffer, OxiRed™Probe, Dimethylsulfoxide, Enzyme Mix,NH4ClStandardandanInstructionManual.

Ascorbic Acid Assay Kit

AscorbicAcidAssayKit Cat.#K661-100 100assays

AscorbicAcid(VitaminC)playsanimportantroleinmanybiologicalprocesses.Itisapotentanti-oxidant,anti-inflammatory,anti-viralagentandanimmunestimulantandpresentinawidevarietyoffoodsandbiologicalspecimens.BioVision’sAscorbicAcidAssayKitprovidesarapid,simpleandsensitivemeansofdetectingascorbicacidinvariousbiologicalsamples.Inthisassay,ourproprietarycatalystoxidizesascorbicacidtoproduceaproductthatinteractswiththeascorbicacidprobe,generatingcolorandfluorescence.Ascorbicacidcanbeeasilydeterminedbyeithercolorimetric(atλmax=570nm)orfluorometric(Ex/Em=535/587nm)methods.Theassaycandetect0.01-10nmolofascorbicacidperassayinvarioussamples.

Kit Contains: Ascorbic Acid Assay Buffer, Ascorbic Acid Probe, Dimethylsulfoxide,Catalyst,AscorbicAcidEnzymeMix,AscorbicAcidStandardandanInstructionManual.

0

0.2

0.4

0.6

0.8

1

0 2 4 6 8 10

OD(570

nm)

nmol ascorbate

Amylase Assay Kit

AmylaseAssayKit Cat.#K711-100 100assays

Amylasesareenzymesthatbreakstarchdowntosugarmolecules.a-Amylaseisthemajorformofamylasefoundinhumansandothermammalsaswellasanenzymepresent inseeds, or in fungi (bakers yeast for instance). a-Amylase is a calcium metalloenzyme,completelyunabletofunctionintheabsenceofcalcium.Inhumanphysiology,boththesalivaryandpancreaticamylasesaremajordigestiveenzymes.Increasedenzymelevelsinhumansareassociatedwithsalivarytrauma;mumpsduetoinflammationofthesalivaryglands,pancreatitisandrenalfailure.BioVision’sa-amylaseAssayusesethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by a-amylase,thesmaller fragmentsproducedcanbeacteduponbyα-glucosidase,whichcausestheultimatereleaseofthechromophorethatcanthenbemeasuredat405nm.Theassaycandetecta-amylasecontentaslowas0.2mU.

Kit Contains:AmylaseAssayBuffer,AmylaseSubstrateMix,AmylasePositiveControl,NitrophenolStandardandanInstructionManual.

y = 0.0365x + 0.0315R² = 0.9992

0

0.2

0.4

0.6

0.8

0 5 10 15 20

O.D. 4

05nm

Nitrophenol (nmol)

Nitrophenol Standard Curve

0

0.2

0.4

0.6

0 20 40 60

O.D. 405

nm

Time (min)

Sample Test 5µl urine

Posit. Ctrl.

0.5µl rabbitserum

C F L

C F L

C F L


Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 11

Ascorbic Acid Assay Kit II (FRASC)

AscorbicAcidAssayKitII(FRASC) Cat.#K671-100 100assays

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is apotentanti-oxidantpresentinawidevarietyofbiologicalspecimens.Duetothepresenceofavarietyofotherantioxidantsinbiologicalsamplessuchasserum,mostascorbicacidassays show strong interference. BioVision’s FRASC Assay Kit provides a rapid, sensitiveandspecificdetectionofascorbicacidinbiologicalsamples.Inthisassay,Fe3+isreducedtoFe2+byanyantioxidantspresentinsample.Theferrousironischelatedwithaprobetoproduceaproductwithstrongabsorbanceat545-575nm.Additionofascorbateoxidasetoparallelsamplesremovesascorbatefromsampleleavingabackgroundvaluewhichissubtractedfromthetotaltogiveascorbatecontent.Theassaycandetect0.2to20nmolofascorbicacidinvarioussamples.

y = 0.1183x 0.0063

0

0.2

0.4

0.6

0.8

1

1.2

0 2 4 6 8 10

OD593n

m

nmol Ascorbate

Kit Contains: FRASC Buffer, Ascorbic Acid Probe, FeCl3 Solution, Ascorbate Oxidase,AscorbicAcidStandardandanInstructionManual.

ATP Colorimetric and Fluorometric Assay Kit

ATPColorimetricandFluorometricAssayKit Cat.#K354-100 100assays

ATP is the primary energy currency of living systems. Virtually all energy requiringprocessesutilizethechemicalenergystoredinthephosphatebondofATP.Commerciallyavailable ATP assays can detects femtomoles or less of ATP by measuring luminescence(BioVision Kit 254-200, for example) but these kits require specialized luminescenceinstrumentationandutilizeluciferasewhichcanbedifficulttomaintaininactiveform.BioVision’s newly developed ATP Colorimetric and Fluorometric Assay kit is designed tobearobust,simplemethodwhichutilizesthephosphorylationofglyceroltogenerateaproductthatiseasilyquantifiedbycolorimetric(λmax=570nm)orfluorometric(Ex/Em=535/587nm)methods.Theassaycandetectaslowas50picomol(1µM)ofATPinvarioussamples.Thekitprovidessufficientreagentsfor100assays.

Kit Contains:ATPAssayBuffer,ATPProbe,Dimethylsulfoxide,ATPConverter,DeveloperMix,ATPStandardandanInstructionManual.

y = 0.137x 0.006

0

0.4

0.8

1.2

0 2 4 6 8 10

OD57

0nm

ATP (nmol)

y = 8383.x 37.23

0

1.5

3

4.5

6

7.5

9

0 0.2 0.4 0.6 0.8 1

RFU(x1000)

ATP (nmol)

ATP Cell Viability Assay Kit

ATPCellViabilityAssayKit Cat.#K254-200 200assays

Cell death is an energy-dependent process that requires ATP. As ATP levels fall to apointwherethecellcanno longerperformbasicmetabolic functions,thecellwilldie.Therefore,lossofATPlevelincellhasbeenusedasanindicatorofcelldeath.Incontrast,cell proliferation has been recognized by increased levels of ATP. The ATP Cell ViabilityAssayKitutilizesluciferasecatalyzedreactionforarapid,bioluminescentdetectionoftheATPlevelstoscreencellviability.Theassaycanbefullyautomaticforhigh-throughput(10seconds/sample)andisextremelysensitive(detects10-100mammaliancells/well).TheassaycanalsobeusedfordetectingATPproductioninvariousenzymaticreactions,aswellasfordetectinglowlevelbacterialcontaminationinsamplessuchasblood,milk,urine,soil,andsludge.

Kit Contains: Nucleotide Releasing Buffer, ATP Monitoring Enzyme, EnzymeReconstitutionBuffer,ATPandanInstructionManual.

U x

104 )

vels

(RL U

ATP

Le

- Act.D Camp. CHX Dex. Etop.

ATP Standard Calibration CurveATP Standard Calibration Curve107

105

106

105

104

103

102

ATP (ng/100 ul)

0 0.01 0.1 1.0 10 100

10

ATP (ng/100 ul)

C F L

C F L

C F L


1� ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Calcium Colorimetric Assay Kit

CalciumColorimetricAssayKit Cat.#K380-250 250assays

Calciumisessentialforalllivingorganisms,whereCa2+sequestrationandreleaseintoand out of the cytoplasm functions as a signal for many cellular processes. 99% ofcalcium is found in bones and teeth with the remaining 1% found in the blood andsoft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and anyvariationoutsidethisrangecanhaveseriouseffects.Calciumplaysaroleinmediatingthe constriction and relaxation of blood vessels, nerve impulse transmission, musclecontractionandhormonesecretion.BioVision’sColorimetricCalciumAssayKitutilizesthechromogeniccomplex(λmax=575nm)formedbetweencalciumionsando-cresolphthaleintoprovideasimpleassayinthephysiologicallyimportantrangeofcalciumconcentration0.4-100mg/dL(0.1-25mM).

y = 0.8847x + 0.0098

0

0.4

0.8

1.2

1.6

0 0.4 0.8 1.2 1.6 2

OD575nm

Calcium Amount (µg/well)

Kit Contains: Calcium Assay Buffer, Chromogenic Reagent, Calcium Standard and anInstructionManual.

Branched Chain Amino Acid Assay Kit

BranchedChainAminoAcidAssayKit Cat.#K564-100 100assays

The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linearaliphatic side-chains, namely leucine, isoleucine and valine. These three essentialaminoacidsmakeupapproximately1/3ofskeletalmuscleinthehumanbody.BCAA’sare currently used clinically to aid in the recovery of burn victims, as well as forstrength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulinsecretion.TheBCAA’shavealsobeenimplicatedinawiderangeofotherphysiologicaleffects.BioVision’sBCAAAssayKitprovidesa simpleconvenientmeansofmeasuringtheBCAA’sinavarietyofbiologicalsamples.ThekitutilizesanenzymeassayinwhichBCAAisoxidativelydeaminated,producingNADHwhichreducestheprobe,generatingacoloredproduct(λmax=450nm).ThekitmeasuresBCAA’sintherangeof0to10nmolwithadetectionlimitof~0.2nmol(~10µMBCAAinsample).

Kit Contains:BCAAAssayBuffer,BCAAEnzymeMix,WSTSubstrateMix,LeuStandardandanInstructionManual.

y = 0.0684x 0.0064

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0 2 4 6 8 10

OD

450n

m

BCAA (nmol/well)

C F L

C F L

Catalase Assay Kit

CatalaseAssayKit Cat.#K773-100 100assays

Catalase (EC 1.11.1.6) is a ubiquitous antioxidant enzyme that is present in nearly alllivingorganisms.Itfunctionstocatalyzethedecompositionofhydrogenperoxide(H202)to water and oxygen. BioVision’s Catalase Assay Kit provides a highly sensitive, simple,directandHTS-readyassayformeasuringCatalaseactivityinbiologicalsamples. Intheassay,catalasefirsteactswithH2O2toproducewaterandoxygen,theunconvertedH2O2reactswithOxiRed™probetoproduceaproduct,whichcanbemeasuredatλmax=570nm(Colorimetricmethod)oratEx/Em=535/587nm(fluorometricmethod).Thekitdetectshighpico-unitofcatalaseinsamples.

y = 0.1309x + 0.0214R² = 0.9967

0

0.5

1

1.5

0 2 4 6 8 10

O.D. 570

H2O2 (nmol)

H2O2 Standard Curve

0

0.5

1

1.5

S1 S2 S3

O.D. 5

70nm

Samples

Sample Test

High Control

SampleKit Contains: Catalase Assay Buffer, OxiRed™ Probe, DMSO, HRP, H2O2, Stop Solution,CatalasePositiveControlandanInstructionManual.

C F L


Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 1�

CETP Activity Assay Kit

CETP Inhibitor Drug Screening Kit

CETPActivityAssayKit Cat.#K601-100 100assays

CETPInhibitorDrugScreeningKit Cat.#K602-100 100assays

Cholesterylestertransferprotein(CETP)isamemberofthelipidtransfer/lipopolysaccharidebindingproteingenefamily.CETPtransfersneutrallipidsfromhigh-densitylipoprotein(HDL)toverylow-densitylipoprotein(VLDL)andispresentinnormalhumanplasmaandserum.TheCETPActivityAssayKitusesadonormoleculecontainingafluorescentself-quenchedneutrallipidthatistransferredtoanacceptormoleculeinthepresenceofCETP.CETP-mediatedtransferofthefluorescentneutral lipidtotheacceptormoleculeresultsinanincreaseinfluorescence(Excitation:465nm;Emission:535nm).

Cholesterylestertransferprotein(CETP)isamemberofthelipidtransfer/lipopolysaccharidebindingproteingenefamily.CETPtransfersneutrallipidsfromhigh-densitylipoprotein(HDL)toverylow-densitylipoprotein(VLDL)andispresentinnormalhumanplasmaandserum.TheCETPDrugScreeningKitusesadonormoleculecontainingafluorescentself-quenchedneutrallipidthatistransferredtoanacceptormoleculeinthepresenceofCETP(rabbit serum). CETP-mediated transfer of the fluorescent neutral lipid to the acceptormolecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).Inhibitors of CETP will inhibit the lipid transfer and therefore decrease fluorescenceintensity.Thekitprovidessufficientreagentsfor100CETPinhibitorscreeningassays.

Kit Contains: Donor Molecule, Acceptor Molecule, CETP Assay Buffer, Positive ControlandanInstructionManual.

Kit Contains:DonorMolecule,AcceptorMolecule,CETPAssayBuffer,RabbitSerumandanInstructionManual.

C F L

C F L

L-Carnitine Assay Kit

L-CarnitineAssayKit Cat.#K642-100 100atssays

Carnitine is a quaternary ammonium compound biosynthesized from the amino acidslysineandmethionine. It is required for transportof fattyacids into themitochondrialmatrix via the carnitine/acylcarnitine shuttle where β-oxidation occurs, acetate isgeneratedandtheacetateutilizedintheTCAcycleforthegenerationofenergy.Carnitineexistsintwostereoisomers.OnlyL-carnitineisbiologicallyactive.BioVision’sL-CarnitineAssayKitprovidesasimpleconvenientmeansofmeasuringfreeL-Carnitineinbiologicalsamples such as serum. The assay transfers an acetyl group from Ac-CoA to carnitineandthefreeCoAformedisfurtherprocessedwithsubsequentoxidationoftheOxi-Redprobetogivefluorescence(Ex/Em=535/587nm)andabsorbance(λmax=570nm).Thedetectionsensitivityforfluorometricassayis~1μM.Fluorometricassayis~10Xmoresensitivethancolorimetricassay.

y = 0.131x + 0.0085

0

0.2

0.4

0.6

0.8

1

1.2

0 2 4 6 8 10

OD57

0nm

nmol L Carnitine

Kit Contains: Carnitine Assay Buffer, Carnitine Probe, DMSO, Carnitine ConvertingEnzyme, Carnitine Substrate Mix, Carnitine Development Mix, Carnitine Standard, andanInstructionManual.

C F L



Cholesterol/Cholesteryl Ester Quantification Kit

Choline/Acetylcholine Quantification Kit

CholesterolAssayKit Cat.#K603-100 100assays

Choline/AcetylcholineQuantificationKit Cat.#K615-100 100assays

The Cholesterol/Cholesteryl Ester Quantification Kit provides a simple method forsensitive quantification of cholesterol and/or cholesteryl ester. A large portion of thecholesterolinbloodisintheformofcholesterylesters.Cholesterolesterasehydrolyzescholesteryl ester into cholesterol. Cholesterol is then oxidized by cholesterol oxidaseto yield H2O2, which interacts with a sensitive cholesterol probe to produce resorufin,thatcanbedetectedbyspectrophotometryatλmax=570nmorfluorometryatEx/Em=535/587nm.Theassaycandetectcholesterolitself(withoutaddingcholesterolesterase)ortotalcholesterol(cholesterol+cholesterylester)byaddingcholesterolesterasetothereaction,orcholesterylesteritselfbysubtractingthevalueofcholesterolfromthetotalvalueofcholesterolandcholesterylesters.

Cholineandacetylcholineplayimportantrolesinmanybiologicalprocesses.BioVision’sCholine/Acetylcholine Quantification Kit provides a simple and sensitive means forquantifyingCholineandAcetylcholinebyeitheracolorimetricor fluorometricmethod.In theassay freecholine isoxidized tobetaine,via the intermediatebetainealdehyde.The reaction generates products which react with the Choline Probe to generate color(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Acetylcholinecanbeconvertedto choline by adding acetylcholinesterase to the reaction. The kit can detect cholineandacetylcholine(totalcholine–freecholine) invariousbiologicalsamplessuchas inblood,cells,culturemedia,fermentationmedia,etc.Thereisnoneedforpretreatmentorpurificationofsamples.Thekitcandetect10pmol-5nmolofcholineoracetylcholine.

Kit Contains:CholesterolReactionBuffer,CholesterolProbe,Dimethylsulfoxide,EnzymeMix,CholesterolEsterase,CholesterolStandardandanInstructionManual.

Kit Contains: Choline Assay Buffer, Choline Probe, DMSO, Choline Enzyme Mix,Acetylcholinesterase,CholineStandardandanInstructionManual.

0.6

0.8

1

1.2

0

0.2

0.4

0 2 4 6 8 10 12

A. Colorimetric Assay

Standard Cholesterol (ug/well)

O.D

.570

nm

6 0

8.0

10.0

12.0

14.0

0.0

2.0

4.0

6.0

0.0 0.2 0.4 0.6 0.8 1.0 1.2

B. Fluorometric Assay

RFU

(x10

^3)

Standard Cholesterol (ug/well)

0

0.7

1.4

2.1

0 1 2 3 4 5

OD

570

nm

Choline (nmol/well)

Chloride Assay Kit

ChlorideAssayKit Cat.#K530-100 100assays

Chlorideisthemostcommonanionfoundinlivingorganisms.Chlorideionsplayavarietyof important physiological roles. Chloride channels are found in a variety of cells andareresponsibleforsettingrestingcellmembranepotentialandregulatingcellvolume.Chloride is also instrumental in maintaining the acid-base balance in blood. A numberof pathologies are associated with defective chloride transport; the most well-knownbeing Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.BioVision’s Chloride Assay Kit provides a quick, simple method for quantification ofChloride inavarietyofbiological samples.Theassay isbaseduponthecompetitionofHg++andFe++forTPTZ.ThepreferredHg-TPTZadductexhibitsnocolor.InthepresenceofChloride,Hg++formsHgCl2freeingupTPTZwhichthenbindstheavailableFe++givingaveryintenseabsorbancewithaλmax~620nm.Theassayislinearintherange20to120nmolChloride/wellwithdetectionsensitivity~0.4mMchloride.

Kit Contains:ChlorideReagent,ChlorideStandardandanInstructionManual.

y = 0.0224x 0.0263

0

0.5

1

1.5

2

2.5

3

0 20 40 60 80 100 120

OD62

0nm

Chloride (nmol)

C F L

C F L

C F L



Citrate Assay Kit

CitrateAssayKit Cat.#K655-100 100assays

Citric acid is a key intermediate in the TCA cycle which occurs in mitochondria. It isformed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived fromthe glycolytic pathway. Citrate can be transported out of mitochondria and convertedback to acetyl CoA for fatty acid synthesis. Citrate is widely used industrially in foods,beveragesandpharmaceuticals.Citratemetabolismanddispositioncanvarywidelyduetosex,ageandavarietyofotherfactors.BioVision’sCitrateAssayKitprovidesasimple,sensitive and rapid means of quantifying citrate in a variety of samples. In the assay,citrateisconvertedtopyruvateviaoxaloacetate.Thepyruvateisquantifiedbyconvertinganearlycolorlessprobetoanintenselycolored(λmax=570nm)andfluorescent(Ex/Em=535/587nm)product.TheCitrateAssayKitcandetect0.1to10nmoles(~2µM-10mM)ofcitrateinavarietyofsamples.

y = 0.126x 0.0131

0

0.4

0.8

1.2

0 2 4 6 8 10

OD

570n

m

Citrate (nmol) /well

Kit Contains:CitrateAssayBuffer,DMSO,CitrateProbe,CitrateEnzymeMix,Developer,CitrateStandardandanInstructionManual.

Coenzyme A Assay Kit

CoenzymeAAssayKit Cat.#K367-100 100assays

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid and ATP.It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidationin the citric acid cycle and many other biological processes. One of the main function ofCoA is the carrying and transfer of acyl groups. One of the most important acyl groupstransferredistheacetategroup,inwhichcasethemoleculeiscalledacetylCoA.Theacetylgroup eventually finds itself incorporated into a variety of molecules such as cholesterol,acetylcholine,melatonin,hemeandtheTCAcycleintermediates.BioVisionhasdevelopedaneasy,convenientassaytomeasuretheCoAlevelinvarietybiologicalsamples.Intheassay,CoAisspecificallyutilizedtogenerateproductswhichreactwithOxiRed™Probetogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Theassaycandetect0.1to10nmolofCoA(2.5-250μMconcentrationrange)inavarietyofsamples.

Kit Contains: CoA Assay Buffer, OxiRed™Probe , DMSO, Conversion Enzyme Mix, CoASubstrate,AcylCoADeveloper,CoAStandardandanInstructionManual.

y = 0.167x 0.011

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

OD570n

m

nmol CoASH

Cobalt Assay Kit

CobaltAssayKit Cat.#K505-100 100assays

Cobalt is an essential trace-element for all multicellular organisms as the active centerof coenzymes called cobalamins. These include vitamin B-12 which is essential for plantsandanimals.Cobaltisalsoanactivenutrientforbacteria,algae,andfungi,andmaybeanecessarynutrient forall life.Cobalt,particularly theCo2+species, formscomplexeswithalargenumberoforganicandinorganicmoleculeswitharangeofextinctioncoefficientsin the visible range. The Cobalt Assay kit takes advantage of the reaction of Co2+ withmercaptoethanol in basic media. Under the condition, Cobalt forms a complex with astrongabsorbancebandat475nm.ThekitprovidesaquickandsimpleaccuratemethodofquantitatingCo2+inavarietyofsamples.Theassayislinearintherange3to50nmol(0.18-3µg)Cobalt,withadetectionlimitof~3nmol(or~15µM,0.18µg).

Kit Contains:CobaltReagent,CobaltChlorideStandardandInstructionManual

y = 0.0238x + 0.022y = 0.0001x + 0.039

y = 0.0015x + 0.037

y = 0.0018x + 0.031

y = 0.0006x + 0.038

y = 0.0001x + 0.037

0

0.2

0.4

0.6

0.8

1

1.2

0 10 20 30 40 50

OD475nm

nmol

Co+2Cu+2

Fe+2

Ni+2

Mn+2

Zn+2

C F L

C F L

C F L



Creatine Assay Kit

Creatinine Assay Kit

CreatineAssayKit Cat.#K635-100 100assays

CreatinineAssayKit Cat.#K625-100 100assays

CreatineisanendogenouscompoundwhosefunctionistomaintainahighATP/ADPratio,bywayofitsphosphorylatedformandcreatinekinase.Creatinesupplementationhasbeenusedinthetreatmentofmuscular,neurologicalandneurodegenerativediseases,aswellas a sport performance enhancer. Detection of creatine levels has wide applications inresearchanddevelopment.BioVision’sCreatineAssayKitprovidesanaccurate,convenientmeasurement of creatine in a variety of biological samples. In the assay, creatine isenzymatically converted to sarcosine which is then specifically oxidized to generate aproductthatconvertsacolorlessprobetoanintenselyredcolor(λmax=570nm)andhighlyfluorescent(Ex/Em=538/587nm)product.Creatineisthereforeeasilydetectedbyeithercolorimetricorfluorometricmethods.Detectionrange0.001–10mMCreatine.

Creatinine is a breakdown product of creatine phosphate. Creatinine is produced andexcretedataconstantrateandbloodcreatinineisusedtodetermineglomerularfiltrationrate (GFR), a measure of kidney function. Blood creatinine levels increase only in casesof significant (>75%) damage to nephrons. BioVision’s Creatinine Assay Kit provides anaccurate,convenientmeasureofcreatineconcentrationinbiologicalfluidssuchasserum,urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatineis converted to sarcosine, which is then specifically oxidized to produce a product thatreacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em =538/587nm).Unlikethepicricacidassay,thiskitissuitableforserum/plasmacreatininedeterminations,aswellasforurineandotherbiologicalsamples.

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

OD570nm

Creatinine (nmol)

Kit Contains:CreatineAssayBuffer,CreatineProbe,DMSO,Creatinase,CreatineEnzymemix,CreatineStandardandanInstructionManual.

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

OD57

0nm

Creatine (nmol)

y = 0.1524x + 0.0262

0

20

40

60

80

0 0.2 0.4 0.6 0.8 1

RFU(X10

3 )

Creatinine (nmol)

Kit Contains:CreatinineAssayBuffer,CreatinineProbe,DMSO,Creatinase,Creatininase,CreatinineEnzymeMix,Creatinine,andanInstructionManual.

C F L

C F L

Ethanol Assay Kit

EthanolAssayKit Cat.#K620-100 100assays

Alcohol (ethanol) is among the most widely consumed drinks. Low doses of alcoholconsumptionmayhelpbloodcirculation,whileheavyalcoholconsumptionmayleadtovariousformsofdiseases.Quantitativedeterminationofalcoholfindsapplicationsinbasicresearch,drugdiscovery,clinicalstudiesandwineindustryprocesses.TheEthanolAssayKitprovidesasimple,rapidandsensitivemethodforaccuratequantificationofethanol concentration in many types of biological samples, such as serum, plasma,bodyfluid,wine,foodorgrowthmedia.Inthisassay,alcoholoxidaseoxidizesethanolto generate H2O2 that reacts with ethanol probe to generate color and fluorescence,whichcaneasilybequantifiedbyeithercolorimetric(λmax=570nm)orfluorometric(Ex/Em=535/587nm)methods.Thekitdetects0.4-40ppmalcohol.

Kit Contains: Ethanol Assay Buffer, Ethanol Probe (lyophilized), Dimethylsulfoxide,EthanolEnzymeMix,EthanolStandardandanInstructionManual.

0

0.4

0.8

1.2

1.6

2

0 8 16 24 32 40Ethanol Standard (nmol/well)

C F L



FAD Assay Kit

Free Fatty Acid Assay Kit

FADAssayKit Cat.#K357-100 100assays

FreeFattyAcidAssayKit Cat.#K612-100 100assays

Flavin Adenine Dinucleotide (FAD) is a redox cofactor which plays an important role inmetabolism. FAD exists in different redox states and cycles between FAD, FADH andFADH2. The primary sources of reduced FAD in eukaryotic metabolism are the citricacid cycle and the beta oxidation reaction pathways. In BioVision’s FAD Assay Kit, FADfunctionsasthecofactorofanoxidasewhichcatalyzestheformationofaproductthatreacts with OxiRed™probe generating color and fluorescence. FAD can be detected byeithercolorimetric(λmax=570nm)orfluorometric(Ex/Em=535/587nm)methods.Thekitprovidesarapid,simple,ultra-sensitiveandreliabletestsuitableforhigh-throughputassayofFAD.Thelowerlimitofdetectionislessthan1nMFAD.

Fatty Acids play very important roles in normal metabolism and in the developmentof many diseases. They are precursors to a number of bioactive classes of compoundsand have been implicated in diverse functions such as autism, immune system andinflammationresponse.BioVision’sFattyAcidQuantificationKitprovidesaconvenient,sensitiveenzyme-basedmethodfordetectingthelong-chainfreefattyacidsinvariousbiologicalsamples,suchasserum,plasmaandotherbodyfluids,food,growthmedia,etc.Intheassay,FattyAcidsareconvertedtotheirCoAderivatives,whicharesubsequentlyoxidizedwiththeconcomitantgenerationofcolororfluorescence.Fattyacidscanthenbeeasilyquantifiedbyeithercolorimetric(atλmax=570nm)orfluorometric(atEx/Em=535/587nm)methods.

0

0.6

1.2

1.8

0 2 4 6 8 10

O.D

.(570

nm

)

Palmitic Acid (nmol/well)

y = 0.6976x + 0.0315R² = 0.9994

0

0.5

1

1.5

0 0.5 1 1.5 2

O.D.570

nm

FAD (pmol)

FAD Colorimetric Assay

y = 45285x + 835.62R² = 0.9996

0

2000

4000

6000

8000

10000

0 0.05 0.1 0.15 0.2

RFU

FAD (pmol)

FAD Fluorometric assay

Kit Contains:FADAssayBuffer,OxiRed™Probe,Dimethylsulfoxide,FADEnzymeMix,FADStandard,StopSolutionandanInstructionManual.

Kit Contains:FattyAcidAssayBuffer,FattyAcidProbe,Dimethylsulfoxide,ACSReagent,EnzymeMix,Enhancer,PalmiticAcidStandardandanInstructionManual.

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Free Glycerol Assay Kit

GlycerolAssayKit Cat.#K630-100 100assays

y = 0.1746x + 0.0103

0

0.4

0.8

1.2

1.6

2

0 2 4 6 8 10

OD 5

70nm

Glycerol (nmol/per well)

Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmeticproducts. There is broad interest in quantification of glycerol for research anddevelopment. BioVision’s Glycerol Assay Kit provides a sensitive, easy assay to measurefree glycerol concentration in various samples. In the assay, glycerol is enzymaticallyoxidized togenerateaproductwhich reactswith theprobeprovided togeneratecolor(λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). Thus glycerol can be easilyquantifiedbycolorimetricandfluorometricmethods.Thekitcandetect50pmol-10nmol(or1~10000μMrange)ofglycerolinvarioussamples.

Kit Contains:GlycerolAssayBuffer,GlycerolProbe,Dimethylsulfoxide,GlycerolEnzymeMix,GlycerolStandardandanInstructionManual.

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Fructose Assay Kit

FructoseAssayKit Cat.#K619-100 100assays

Fructose is a monosaccharide found in many foods and is one of the three mostimportantbloodsugarsalongwithglucoseandgalactose.Fructose is thesweetestnaturally occurring sugar, estimated to be twice as sweet as sucrose. In BioVision’sFructose Assay Kit, free fructose is enzymatically converted to β-glucose, whichis then specif ically oxidized to generate a product that reacts with OxiRed™Probeto generate color (λmax = 570 nm) and f luorescence (Ex/Em = 535/587 nm). Thekit provides a rapid, simple, sensitive and reliable method also suitable for high-throughputassayofD-fructose.Thedetectionlimitis~1mM.

y = 0.0733x + 0.0004R² = 0.9978

0

0.2

0.4

0.6

0.8

0 2 4 6 8 10

O.D.570

nm

Fructose Amount (nmol/well)

y = 5025.x + 59.72R² = 0.996

0

1000

2000

3000

4000

5000

0 0.2 0.4 0.6 0.8 1

RFU

Fructose Amount (nmol/well)

Kit Contains:FructoseAssayBuffer,OxiRed™Probe,Dimethylsulfoxide,EnzymeMix,FructoseConvertingEnzyme,FructoseStandardandanInstructionManual.

Galactose and Lactose Assay Kit

GalactoseandLactoseAssayKit Cat.#K617-100 100assays

Galactoseandlactosearenaturallyoccurringsugars.Lactoseconsistsofonegalactoseand one glucose. Some people, particularly infants, lack the enzyme necessary todigestgalactoseleadingtogalactoseaccumulationinblood(Galactosemia)causingenlarged liver, renal failure, cataracts and brain damage. BioVision’s Galactose andLactoseAssayKitprovidesasimple,convenientmeansformeasurementofgalactoseand lactose levels in various biological samples. To detect Galactose, galactoseoxidase specif ically oxidizes free galactose, generating a product that reacts withthe Galactose Probe to produce color (OD = 570 nm) and f luorescence (Ex/Em =535/587nm). Inthe lactoseassay, lactose ishydrolyzedby lactasetogenerate freegalactose that is then measured. Thus, the Lactose level = Total Galactose – FreeGalactose.

Kit Contains: Galactose Assay Buffer, Galactose Probe, Dimethylsulfoxide, Lactase,GalactoseEnzymeMix,GalactoseStandardandanInstructionManual.

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Galactose Assay Kit

GalactoseAssayKit Cat.#K621-100 100assays

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

OD57

0nm

Galactose Amount (nmol/well)

Galactose is one the major naturally occurring sugars, involved in many biologicalprocesses. BioVision’s Galactose Assay Kit provides a simple, convenient means fordirectmeasurementofgalactose levels invariousbiologicalsamples(serum,plasma,other body fluids, food, growth media, etc.). In the assay, Galactose is specificallyoxidized generating a product that reacts with the Galactose Probe to produce bothcolor(OD=570nm)andfluorescence(Ex/Em=535/587nm).Galactosecanbeeasilyquantifiedusingcolorimetricandfluorometricmethods.Liquidsamplescanbetesteddirectlywithoutpurification.Theassayisfast,convenientandsensitive.Itcanalsobeusedinhigh-throughputassay.

Kit Contains:GalactoseAssayBuffer,GalactoseProbe,Dimethylsulfoxide,GalactoseEnzymeMix,GalactoseStandardandanInstructionManual.

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Glucose Assay Kit

GlucoseAssayKit Cat.#K606-100 100assays

Glucose is a very important fuel source to generate the universal energy molecule ATP.Glucoselevelisakeydiagnosticparameterformanymetabolicdisorders.Measurementofglucosecanbeveryimportantinbothresearchanddrugdiscoveryprocesses.TheGlucoseAssay Kit provides direct measurement of glucose in various biological samples (e.g.,serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specificallyoxidizesglucosetogenerateaproductwhichreactswithadyetogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Thegeneratedcolorandfluorescenceisproportional to the glucose amount. The method is rapid, simple, sensitive and suitablefor high-throughput. The assay is also suitable for monitoring glucose level duringfermentationandglucosefeedinginproteinexpressionprocesses.Thekitdetects1-10000μMglucose.

y = 0.128x + 0.010

0

0.5

1

1.5

0 2 4 6 8 10

OD57

0nm

Glucose (nmol/well)

Kit Contains: Glucose Assay Buffer, Glucose Probe, Dimethylsulfoxide, GlucoseEnzymeMix,GlucoseStandardandanInstructionManual.

Glucose and Sucrose Assay Kit

GlucoseandSucroseAssayKit Cat.#K616-100 100assays

Glucose and sucrose are the important fuel sources to generate universal energymoleculeATP. Sucrose isadisaccharidewhichcanbeconvertedintooneglucoseandonefructosewhenaddingInvertase.BioVision’sGlucoseandSucroseAssayKitprovidesa convenient means for measuring glucose and sucrose levels from various biologicalsamples. To measure glucose, glucose oxidase specifically oxidizes free-glucosegenerating a compound that reacts with the glucose probe to produce color (OD =570nm)orfluorescence(Ex/Em=535/587nm).Tomeasuresucrose,invertasecanbeaddedtothereactiontoconvertsucrosetofreeglucoseandfructose,sototalglucoselevelcanbemeasured.Thenthesucroselevel=TotalGlucose–FreeGlucose.

Kit Contains: Glucose Assay Buffer, Glucose Probe, Dimethylsulfoxide, Invertase,GlucoseEnzymeMix,SucroseStandardandanInstructionManual.

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

OD57

0nm

Galactose Amount (nmol/well)

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Glucose-6-Phosphate Assay Kit

Glucose-6-PhosphateAssayKit Cat.#K657-100 100assays

Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cellsandthenentereitherthemetabolicpathwaysorstorage.G6Pcanentertheglycolyticpathway,thepentosephosphateshuntorbestoredasglycogenorstarch.G6Pisutilizedby its dehydrogenase to generate reducing equivalents in the form of NADPH. This isparticularlyimportantinredbloodcellswhereaG6PDHdeficiencyleadstohemolyticanemia. BioVision’s glucose-6-phosphate Assay provides a simple, sensitive and rapidmeans of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphateisoxidizedtogenerateaproductthatconvertsanearlycolorlessprobetoanintenselycoloredproductwithanabsorbanceat450nm.The levelofglucose-6-phosphatecanthereforebeeasilyquantifiedbycolorimetricmethod.TheAssayKitcandetectG6Pintherangeof1to30nmoleswithdetectionsensitivity~10μMofG6P.

Kit Contains:G6PAssayBuffer,G6PEnzymeMix,G6PSubstrateMix,G6PStandardandanInstructionManual.

y = 0.0604x

0

0.1

0.2

0.3

0.4

0.5

0.6

0 2 4 6 8 10

OD450n

m

G 6 P Amount (nmol/well)

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�0 ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Glutathione Assay Kit (GSH, GSSG and Total)

GlutathioneAssayKit(GSH,GSSGandTotal) Cat.#K264-100 100assays

Commercially available glutathione assay kits hardly distinguish between reducedglutathione (GSH) and oxidized glutathione (GSSG). BioVision’s Glutathione DetectionKit provides a unique, convenient tool for detecting GSH, GSSG and total glutathioneindividually.Intheassay,OPA,reactswithGSH(notGSSG),generatingfluorescence,soGSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so(GSH+GSSG)canbedetermined.TomeasureGSSGspecifically,aGSHQuencherisaddedto remove GSH, preventing reaction with OPA (while GSSG is unaffected). ReducingagentisthenaddedtodestroyexcessquencherandtoconvertGSSGtoGSH.Thus,GSSGcanbespecificallyquantified.ThekitprovidesauniqueprocedureandbufferformulatoeliminateproteinthiolinterferenceandtostabilizeGSHandGSSGinsolution.Theassayiseasytoperformanddetects2-400ng/μlofGSH,GSSGortotalglutathione.

0

1000

2000

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0 0.4 0.8 1.2 1.6 2

Glutathione Amount µg/Well)

RFU

Kit Contains:GlutathioneAssayBuffer,PCA,KOH,OPAProbe,ReducingAgentMix,GSHQuencherGSHStandardandanInstructionManual.

Glutamate Assay Kit

GlutamateAssayKit Cat.#K629-100 100assays

y = 0.073x + 0.122R² = 0.998

0

0.2

0.4

0.6

0.8

1

0 2 4 6 8 10

O.D

. 450

nm

Glutamate (nmol)

Glutamate Standard Curve

Glutamate is a key molecule in cellular metabolism. In humans, glutamate plays animportantrolebothinaminoaciddegradationanddisposalofexcessorwastenitrogen.Glutamate is the most abundant swift excitatory neurotransmitter in the mammaliannervoussystem.Itisbelievedtobeinvolvedinlearningandmemoryandhasappearedto be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, someformsofmental retardationandAlzheimer’sdisease.Glutamicacid isalsopresent inawidevarietyoffoodsandhasbeenusedasaflavorenhancerinfoodindustry.BioVision’sGlutamateAssayKitprovidesasensitivedetectionofglutamateinavarietyofsamples.The glutamate Enzyme Mix recognizes glutamate as a specific substrate leading toproportional color development. The amount of glutamate can therefore be easilyquantifiedbycolorimetric(λmax=450nm)method.

Kit Contains: Glutamate Assay Buffer, Glutamate Enzyme Mix, Glutamate Developer,GlutamateStandardandanInstructionManual.

Glucose-6-Phosphate Dehydrogenase Assay Kit

Glucose-6-PhosphateDehydrogenaseAssayKit Cat.#K757-100 100assays

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme involved in themetabolic pathway that supplies reducing energy to cells by maintaining the level oftheco-enzymeNADPH.TheNADPHinturnmaintainsthelevelofglutathionethathelpsprotecttheredbloodcellsagainstoxidativedamage.BioVision’sglucose-6-phosphatedehydrogenase Assay Kit provides rapid, simple and sensitive assay that detects theactivityofG6PDHinavarietyofsamples.Intheassay,G6PDHdehydrogenateglucose-6-phosphategeneratingaproductwhichconvertanearlycolorlessprobetoanintenselycolored product (λmax = 450 nm). The G6PDH Assay Kit can detect as low as 0.04 mUG6PDHperwell.

Kit Contains: AssayBuffer,G6PDHSubstrate,G6PDHDeveloper,G6PDHPositiveControl,NADHStandardandanInstructionManual. y = 0.0761x + 0.0226

R² = 0.99810

0.5

1

0 5 10

OD45

0nm

NADH Standard (nmol)

NADH Standard Curve

0

0.2

0.4

0.6

0 10 20 30

O.D.

450n

m

Time (min)

Sample TestPstv.Contl

PorkLiverExtra.

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Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 �1

Glutathione Colorimetric Detection Kit

Glutathione Fluorometric Detection Kit

GlutathioneColorimetricDetectionKit Cat.#K261-100 100assays

GlutathioneFluorometricDetectionKit Cat.#K251-100 100assays

0

1000

2000

3000

4000

0 0.2 0.4 0.6 0.8 1

RF

U

Amount of Glutathione ( g/Well)

Glutathione is the major intracellular low-molecular-weight thiol that plays a criticalroleinthecellulardefenseagainstoxidativestress.BioVision’sGlutathioneColorimetricAssay Kit provides a convenient method for analyzing either total or the reduced formof glutathione. The assay is based on the glutathione recycling system by DTNB andglutathionereductase,asshowninthefigure,whichdramaticallyimprovesthesensitivityoftotalglutathionedetection.Thekitcanquantifyglutathionefrom1-100ng/well ina 200 μl reaction. The kit can also specifically detect the reduced form of glutathione(GSH)byomittingtheglutathionereductasefromthereactionmixture.Thesensitivityfordetectingthereducedformofglutathioneis100timeslowerthandetectingthetotalglutathione.

Glutathioneistheprincipalintracellularlow-molecular-weightthiolthatplaysacriticalrole inthecellulardefenseagainstoxidativeandnitrosativestress inmammaliancells.Diminished glutathione levels have been observed in the early stages of apoptosis.BioVision’s Glutathione Fluorometric Detection Kit provides a simple in vitro assay fordetection of total glutathione changes in apoptosis and other samples. The assayutilizesmonochlorobimane(MCB),adyethatappearstoformanadductexclusivelywithglutathione.TheunboundMCBisalmostnonfluorescent,whereasthedyefluorescesblue(Ex/Em = 380/461 nm) when bound to glutathione of reduced or oxidized form. Thus,theamountoftotalglutathionecanbeeasilydetectedusingafluorometerora96-wellfluorometricplatereader.ThereactioniscatalyzedbyglutathioneS-transferase.

Kit Contains:CellLysisBuffer,Monochlorobimane,GSTReagent,GSHStandardandInstructionManual.

Kit Contains: Glutathione Reaction Buffer, Glutathione Substrate, NADPH, GeneratingMix, Glutathione Reductase, Sulfosalicylic Acid, GSH Standard and an InstructionManual.

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Glutathione Peroxidase Assay Kit

GlutathionePeroxidaseAssayKit Cat.#K762-100 100assays

Glutathione Peroxidase (GPx) plays an important role in protecting organisms fromoxidativedamage.Itconvertsreducedglutathione(GSH)tooxidizedglutathione(GSSG),reduces lipid hydroperoxides to their corresponding alcohols, or reduces free hydrogenperoxide to water. Low levels of GPx have been correlated with free radical relateddisorders.BioVision’sGlutathionePeroxidaseAssayKitmeasuresglutathioneperoxidase(GPx)activitythroughacoupledreactionwithglutathionereductase(GR).Intheassay,GPxoxidizesGSHtoGSSG,whichisthenreducedtoGSHwithconsumptionofNADPHbyGR.ThedecreaseofNADPHisproportionaltoGPxactivity inthereaction.Thedecreaseof NADPH can be easily measured by absorbance at 340 nm. The assay can be used tomeasure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates,tissue homogenates and cell lysates with detection sensitivity ~ 0.5 mU/ml of GPx insamples.Kit Contains:GPxAssayBuffer,NADPH,GR,GSH,CumeneHydroperoxide,GPxPositiveControlandInstructionManual

y = 0.0163x + 0.0336R² = 0.99820

0.5

1

1.5

0 20 40 60 80 100

OD340

NADPH (nmol)

NADPH Standard Curve

0.5

0.7

0.9

1.1

0 10 20

OD34

0

Time (min)

0 1mU GPx

0mU

0.2mU

0.4mU

0.6mU

0.8mU

1.0mU

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�� ordering:call 800.891.9699 or650.428.0236 orfax:650.428.0336 oronlineatwww.biovision.com

Glutathione Reductase Assay Kit

GlutathioneReductaseAssayKit Cat.#K761-200 200assays

GlycogenAssayKit Cat.#K646-100 100assays

GlutathioneReductasecatalyzestheNADPH-dependentreductionofoxidizedglutathione(GSSG)toreducedglutathione(GSH),whichplaysanimportantroleintheGSHredoxcyclethat maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential forprotectionagainstoxidativestress.BioVision’sGlutathioneReductaseAssayKitprovidesa highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GRactivity in biological samples. In the assay, GR reduces GSSG to GSH, which then reactswith 5, 5-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax =405nm).ThereforeGRactivitycanbeeasilyquantifiedbycolorimetricmethod.Theassaycandetect0.1–40mU/mlGRinvarioussamples.

Kit Contains: GR Assay Buffer, H2O2, Catalase, TNB Standard, DTNB, NADPH-GNERAT™,GSSG,GRPositiveControlandanInstructionManual.

0.016 mU0.063 mU0.25 mU0.5 mU

1 mU

2 mU

4 mU

8 mU

0

1

2

3

0 5 10 15

O.D.450

nm

Time (minutes)

GR assay time line

y = 0.036x + 0.0626R² = 0.9992

0

0.5

1

1.5

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0 10 20 30 40 50

O.D.

405n

m

TNB Amount (nmol)

TNB Standard Curve

Glycogen Assay Kit

Glycogen is the primary short term energy storage molecule in animals, synthesizedprimarilyintheliverandmuscle.Glycogenisabranchedglucosepolymer,inα-1,4linkage,withbranchingviaα-1,6linkage.Abnormalabilitytoutilizeglycogenisfoundindiabetesandinseveralgeneticglycogenstoragediseases.TheBioVision’sGlycogenAssayKitisaneasyandconvenientassaytomeasureglycogenlevelsinbiologicalsamples.Intheassay,glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized toproduceaproductthatreactswithOxiRed™probetogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Theassaycandetectglycogen0.0004to2mg/ml.

Kit Contains: Hydrolysis Buffer, Development Buffer, OxiRed™ Probe, DMSO, HydrolysisEnzymeMix,DevelopmentEnzymeMix,GlycogenStandardandInstructionManual.

y = 39234x + 40.68

0

2000

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RFU

Glycogen Amount (µg)

y = 0.664x + 0.009

00.20.40.60.81

1.2

0 0.4 0.8 1.2 1.6 2

OD(570nm)

Glycogen Amount (µg)

GST Colorimetric Activity Assay Kit

GSTColorimetricActivityAssayKit Cat.#K263-100 100assays

Glutathione S-transferase (GST) which catalyzes the conjugation of the thiol group ofglutathione to electrophilic xenobiotics, is part of the defense mechanism against themutagenic, carcinogenic and toxic effects of such compounds. The GST ColorimetricActivity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GSTsubstrate,CDNB(1-chloro-2,4-dinitrobenzene),whichhasthebroadestrangeofisozymedetectability(e.g.,alpha-,mu-,pi-,andotherGSTisoforms).Undercertainconditions,theinteractionbetweenglutathioneandCDNBistotallydependentonthepresenceofactiveGST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether whichcanbedetectedbyspectrophotometeratλmax=340nm.ThekitcandetectGSTactivityin crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusionprotein.Detectlimit:ActiveGST10ng/assay.

Kit Contains: GST Assay Buffer, GST Sample Buffer, GST Substrat, Glutathione, GSTPositiveControl,andanInstructionManual.

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Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 ��

GST Fluorometric Activity Assay Kit

GSTFluorometricActivityAssayKit Cat.#K260-100 100assays

Glutathione S-transferase (GST) catalyzes the conjugation of the thiol group ofglutathione to electrophilic xenobiotics and is part of the defense mechanism againstthemutagenic,carcinogenicandtoxiceffectsofsuchcompounds.TheGSTFluorometricAssayKitprovidesasimpleassayfordetectingtheGSTactivityusingmonochlorobimane(MCB),adyethatreactswithglutathione.ThefreeformofMCBisalmostnonfluorescent,whereasthedyefluorescesblue(Ex/Em=380/461nm)whenitreactswithglutathione.GSTcatalyzestheMCB-glutathionereactionsandthefluorescencelevelsareproportionaltotheamountsofGSTpresentinthereaction.GSTlevelinsamplescanbeeasilydetectedincrudecelllysatesorpurifiedproteinfractions.ThekitcanalsoquantitateGST-taggedfusionproteins.

Kit Contains: GST Assay Buffer, GST Sample Buffer, MCB Substrate, Glutathione, GSTStandard,andanInstructionManual.

HAT Activity Assay Kit

HATActivityAssayKit Cat.#K332-100 100assays

Histone acetyltransferases (HATs) have been implicated to play a crucial role in variouscellular functions, such as gene transcription, differentiation, and proliferation.BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactivesystem for a rapid and sensitive detection of HAT activity in mammalian samples. ThekitutilizesactiveNuclearExtract(NE)asapositivecontrolandacetyl-CoAasacofactor.AcetylationofpeptidesubstratebyactiveHATreleasesthefreeformofCoAwhichthenserves as an essential coenzyme for producing NADH. NADH can easily be detectedspectrophotometricallyuponreactingwithasolubletetrazoliumdye.Thedetectioncanbecontinuousandsuitableforkineticstudies.Thekitprovidesasimple,straightforwardprotocolforacompleteassay.

Kit Contains:2XHATAssayBuffer,HATSubstrateI,HATSubstrateII,NADHGeneratingEnzyme,NuclearNuclearExtract,HATReconstitutionBuffer,andanInstructionManual.

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HDAC Colorimetric Assay Kit

HDACColorimetricAssayKit Cat.#K331-100 100assays

Inhibitionofhistonedeacetylases(HDACs)hasbeenimplicatedtomodulatetranscriptionandtoinduceapoptosisordifferentiationincancercells.However,screeningHDACinhibitorycompoundshasproventobedifficultoverthepastduetothe lackofconvenienttools foranalyzingHDACactivity. The new HDAC Activity Assay Kit provides a fast and convenient colorimetric methodthatrequiresonlytwoeasysteps,bothperformedonthesamemicrotiterplate.First,theHDACcolorimetric substrate, which comprises an acetylated lysine side chain, is incubated with asamplecontainingHDACactivity(e.g.,HeLanuclearextractoryourownsamples).Deacetylationofthesubstratesensitizesthesubstrate,sothat, inthesecondstep,treatmentwiththeLysineDeveloper produces a chromophore that can be easily analyzed using an ELISA plate reader orspectrophotometer. The assay is well suited for high-throughput screening applications. HDACinhibitorsandantibodiesarealsoavailableseparately.

0

0.5

1

1.5

OD40

5nm

NE : 0 25 g 50 gTricho. A : + + +

Kit Contains:HDACSubstrate,10XHDACAssayBuffer,LysineDeveloper,HDACInhibitor,HeLaNuclearExtract,DeacetylatedStandardandanInstructionManual.

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HDL and LDL/VLDL Assay Kit

HDLandLDL/VLDLAssayKit Cat.#K613-100 100assays

It is well known that low levels of HDL (high-density-lipoprotein) and high levels ofLDL (low-density-lipoprotein) are associated with an increased risk of cardiovascularevents. BioVision’s HDL and LDL/VLDL Cholesterol Quantification Kit provides a simplequantification of HDL and LDL/VLDL, individually, after a convenient separation of HDLfrom LDL and VLDL in serum samples. In the assay, cholesterol oxidase specificallyrecognizesfreecholesterolandproducesproductstoreactwithprobetogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=538/587nm).Cholesterolesterasehydrolyzescholesteryl ester into free cholesterol. Cholesterol ester and/or free cholesterol can bedetected separately in the fractions by adding or omitting cholesterol esterase in thereactions.

2

0.48

0.75

0.23

0.54

1.4

0.23 0.18

0.42

0

0.5

1

1.5

2

Human Serum Rabbit Serum Mouse Serum

Total CholesterolHDLLDL/VLDL

Cho

lest

erol

Con

cent

ratio

n in

Ser

um (

g/l)

Kit Contains:CholesterolReactionBuffer,2XLDL/VLDLPrecipitationBuffer,CholesterolProbe, Dimethylsulfoxide, Enzyme Mix, Cholesterol Esterase, Cholesterol Standard, andanInstructionManual.

HDAC Fluorometric Assay Kit

HDAC Inhibitor Drug Screening Kit

HDACFluorometricAssayKit Cat.#K330-100 100assays

HDACInhibitorDrugScreeningKit Cat.#K340-100 500assays

TheFluorometricHDACActivityAssayKitprovidesafastandfluorescence-basedmethodthat eliminates radioactivity, extractions, or chromatography, as used in traditionalassays. The new procedure requires only two easy steps, both performed on the samemicrotiter plate. First, the HDAC substrate, which comprises an acetylated lysine sidechain, is incubatedwithasamplecontainingHDACactivity(e.g.,HeLanuclearextract).Deacetylationofthesubstratesensitizesthesubstrate,sothatfurthertreatmentwiththeLysineDeveloperproducesafluorophore.Thefluorophorecanbeeasilyanalyzedusingafluorescenceplatereaderorafluorometer.Theassayiswellsuitedforhigh-throughputscreeningapplications.HDACinhibitorsandantibodiesarealsoavailableseparately.

Inhibitionofhistonedeacetylase(HDAC)hasbeenimplicatedtomodulatetranscriptionandtoinduceapoptosisordifferentiationincancercells.However,screeningofcompoundsfor HDAC inhibition has been difficult due to the lack of convenient tools for analyzingHDACactivity.TheHDACInhibitorDrugScreeningKitprovidesafast,fluorescence-basedmethodthatrequiresonlytwoeasysteps.First,yourinhibitorcandidatesaremixedwithHeLa Nuclear Extract and HDAC fluorometric substrate, which comprises an acetylatedlysinesidechain.Deacetylationofthesubstratesensitizesthesubstrate,sothat, inthesecondstep,treatmentwiththeLysineDeveloperproducesafluorophore.Thefluorophorecanbeeasilyanalyzedusingafluorescenceplatereaderorafluorometer.Theassayiswellsuitedforhigh-throughputscreeningapplications.



RFU

H e L a N E ( g /a ssa y )0 2 4 6 8 1 0

4

6

2

0

8

1 0

1 2

RFU

H e L a N E ( g /a ssa y )0 2 4 6 8 1 0

4

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2

0

8

1 0

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Trichostatin A Inhibits HDAC Activity

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Heme Assay Kit

Hydrogen Peroxide Assay kit

HemeAssayKit Cat.#K672-100 100assays

HydrogenperoxideAssaykit Cat.#K265-200 200assays

Free hemin which results from the breakdown of hemin-containing proteins such ashemoglobinandmyoglobincanbedetected invariousbodyfluidssuchassaliva,urineandCSF.Freeheminexists incellsataveryminimalconcentrations(<1μM≈650ng/ml) exerting regulatory functions such as repression of non specific δ-aminolevulinatesynthase expression and induction of microsomal hemin oxygenase-1. Hemin canstimulate growth of oral bacteria involved in gingivitis and is an indicator of possiblepathological conditions when found in the urine or feces. BioVision’s Hemin Assay Kitutilizes peroxidase activity in the presence of hemin to provide a simple, exquisitelysensitive assay which causes the conversion of a colorless probe to a strongly colored(λmax=570nm)compound.TraceamountsofHemincanbequantitatedinthe5-160pg(10-250fmol)range.

HydrogenPeroxideisareactiveoxygenmetabolicbyproductthatservesasakeyregulatorfor a number of oxidative stress-related states. Functioning through NF-kB and otherfactors, hydroperoxide-mediated pathways have been linked to asthma, inflammatoryarthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neurodegenerativediseases,Down’ssyndromeandimmunesystemdiseases.BioVision’sHydrogenPeroxideAssay Kit provides a highly sensitive, simple, direct and HTS-ready colorimetric andfluorometric assay for measuring H2O2 in biological samples. In the presence of HorseRadishPeroxidase(HRP),theOxiRed™ProbereactswithH2O2toproduceaproductwithcolor (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit allows for 200reactionsbyfluorometricmethodor100reactionsbycolorimetricmethod.Thedetectionlimitis2pmol(or40nM).

0

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1

1.2

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O.D.570nm

Hemin Amount (fmol)

Kit Contains:HeminAssayBuffer,HeminProbe,DMSO,EnzymeMix,HeminSubstrate,HeminStandard,andanInstructionManual.

Kit Contains:H2O2AssayBuffer,OxiRed™Probe,Dimethylsulfoxide,HRP,H2O2StandardandanInstructionManual.

y = 10.795x + 8.6908R² = 0.9955

0

2000

4000

6000

0 100 200 300 400 500

RFU

H2O2 (pmol)

H2O2 Fluorimetric Standard Curve

y =20.15x + 82.178R² =0.9998

0

500

1000

0 20 40

y = 0.2306x + 0.0773R² = 0.9945

0

0.5

1

0 1 2 3 4 5

OD

570

H2O2 (nmol)

H2O2 Colorimetric Standard Curve

y =0.2366x + 0.0419R² =0.999

0

0.05

0.1

0.15

0 0.2 0.4

β-Hydroxybutyrate (β-HB) Assay Kit

β-Hydroxybutyrate(β-HB)AssayKit Cat.#K632-100 100assays

Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels,shuttingoffthesupplyofglucosetothebody.Thephysiologicalresponseisfortheliverto produce ketone bodies (acetoacetate, acetone and primarily β-hydroxybutyrate)fromtheacetylCoAproduced fromfattyacidoxidation.Theveryhighrateofketonebodyproductionoutstripsthebody’sabilitytoutilizethemasanenergysourceandthebloodconcentrationbuildsup, resulting inasignificantdrop inbloodpH.BioVision’sβ-HB Assay kit utilizes β-HB Dehydrogenase to generate a product which reacts withour colorimetric probe with an absorbance band at 450 nm. The kit provides an easyandconvenientassaytomeasureβ-HBlevelsinbiologicalsamples.Theassayislinearfor1-20nmolβ-HBinupto100μlsamplesor0.01-0.2mMofβ-HBsamples.

Kit Contains:β-HBAssayBuffer,β-HBEnzymeMix,β-HBSubstrateMix,β-HBStandardandanInstructionManual.

y = 0.0565x 0.0164

0

0.2

0.4

0.6

0.8

1

1.2

0 4 8 12 16 20

OD45

0nm

nmol Hydroxybutyrate

C F L

C F L

C F L



Iron Assay Kit

IronAssayKit Cat.#K390-100 100assays

Iron is essential to nearly all known organisms. It is generally stored in the centre ofmetalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic ironalsocontributestoredoxreactions intheiron-sulfurclustersofmanyenzymes,suchasnitrogenase and hydrogenase. BioVision’s Iron Assay Kit provides a simple, convenientmeansofmeasuringFerrousand/orFerricioninsample.Intheassay,ferriccarrierproteinwilldissociateferric(Fe3+)intosolutioninthepresenceofacidbuffer.Afterreductiontotheferrousform(Fe2+),ironreactswithFereneStoproduceastablecoloredcomplexandgiveabsorbanceatλmax=593nm.Aspecificchelatechemicalisincludedinthebuffertoblockcopperion(Cu2+)interference.Thekitmeasuresironinthelinearrangeof0.4to20nmolin50μlsample,or8μMto400μMironconcentrationinvarioussamples.

Kit Contains:IronAssayBuffer,IronProbe,IronReducer,IronStandardandanInstructionManual.

0.08

0.12

0.16

0.2

0.24

0 20 40 60

O.D. 5

93nm

Time (min)

Samples Time LineSoil Sample

FBS Sample

0.5nmol Iron

y = 0.0826x + 0.0199R² = 0.9987

0

0.2

0.4

0.6

0.8

0 2 4 6 8 10

O.D. 593

nm

Iron (nmol)

Iron Standard Curve

Isocitrate Assay Kit

Isocitrateassaykit Cat.#K656-100 100assays

Isocitricacid(HOOC-CHOH-CH(-COOH)-CH2-COOH)isanintermediateoftheKrebsTCAcycle,positioned between citrate and α-ketoglutarate. It is the branch point from which theglyoxylateshuntoperatesinplantsandlowerorganisms.Isocitrateisfoundinsubstantialconcentrationsinmanyfruitsandvegetablesaswellasinfoodsproducedfromtheserawmaterials.IntheTCAcycle,isocitrateisoxidizedbyisocitratedehydrogenase(IDH)toα-ketoglutaratewiththegenerationofNAD(P)H.LossofNAD-IDHhasbeenimplicatedasapotentialcausativefactorinretinitispigmentosa.BioVision’sIsocitrateAssayKitprovidesasimple,sensitiveandrapidmeansofquantifying isocitrate inavarietyofsamples. Inthe assay, isocitrate is oxidized with the generation of NADPH which converts a nearlycolorlessprobetoanintenselycoloredspecieswithaλmaxof450nm.TheIsocitrateAssayKitcandetect1to20nmoles(~20mM)ofisocitrateinvarioussamples.

y = 0.071x + 0.001

0

0.4

0.8

1.2

1.6

0 4 8 12 16 20

OD45

0nm

Isocitrate nmol/well

0 1 2 3 4 5Isocitrate µg/well

Kit Contains: IsocitrateAssayBuffer, IsocitrateEnzymeMix,WSTDeveloper, IsocitrateStandard,andanInstructionManual.

α-Ketoglutarate Assay Kit

a-Ketoglutarateassaykit Cat.#K677-100 100assays

y = 0.123x + 0.0340

0.4

0.8

1.2

0 2 4 6 8 10

OD

570

nmol/well

ketoglutarate

00.20.40.60.811.20 0.2 0.4 0.6 0.8 1 1.2 1.4

ug/well

a-Ketoglutarate(a-KG)isakeyintermediateintheKrebscycle,comingafterisocitrateandbeforesuccinylCoA.Anapleroticreactionsreplenishthecyclebysynthesizinga-KGfromtransaminationofglutamate,orthroughtheactionofglutamatedehydrogenase.α-KGisanimportantnitrogentransporter.Beingakeyintermediate,itisoneoftheorganicacidsmeasuredinnewbornsasanindicatorofinbornerrorsofmetabolism.BioVision’sa-KetoglutarateAssayKitprovidesasimple,sensitiveandrapidmeansforquantifyinga-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with thegenerationofpyruvatewhichisutilizedtoconvertanearlycolorlessprobetobothcolor(λmax=570nm)andfluorescence(Ex/Em535/587nm).Thea-KetoglutarateAssayKitisusefulfordetectingα-ketoglutarateintherangeof0.01to10nmoles.

Kit Contains:a-KGAssayBuffer,a-KGprobe,DMSO,a-KGEnzymeMix,a-KGStandardandanInstructionManual.

C F L

C F L

C F L



Lactate Assay Kit II

LactateAssayKitII Cat.#K627-100 100assays

Lactate plays important roles in many biological processes. Abnormally highconcentrationsoflactatehavebeenrelatedtodiseasestatessuchasdiabetesandlacticacidosis,etc.L(+)-Lactateisthemajorlactatestereoisomerformedinhumanintermediarymetabolismandispresentinblood.D(-)-Lactateisalsopresentbutonlyatabout1-5%oftheconcentrationofL(+)-Lactate.IntheLactateAssayKit,lactateisoxidizedbylactatedehydrogenase to generate a product which interacts with a probe to produce a color(λmax=450nm)whichcanbequantifiedbycolorimetricmethod.ThekitdetectsL(+)-Lactateinbiologicalsamplessuchasinserumorplasma,cells,cultureandfermentationmedia. There is no need for pretreatment or purification of samples. It detects 0.02-10mMlactateinvarioussamples.

y = 0.0902x 0.0009

0

0.2

0.4

0.6

0.8

1

0 2 4 6 8 10

OD450nm

nmol lactateKit Contains: Lactate Assay Buffer, Lactate Enzyme Mix, Lactate Substrate Mix, L(+)-LactateStandard,andanInstructionManual.

0.9

1.2

1.5

570 n

m)

0

0.3

0.6

0 2 4 6 8 10

OD

(5

Lactate Amt. (nmol/Well)

Lactate Assay Kit

LactateAssayKit Cat.#K607-100 100assays

Abnormal high concentrations of lactate have been related to disease states such asdiabetesandlacticacidosis,etc.L(+)-Lactateisthemajorstereoisomeroflactateformedinhumanintermediarymetabolismandispresent inblood.D(-)-Lactate isalsopresentbut only at about 1-5% of the concentration of L(+)-Lactate. In the Lactate Assay Kit,L-lactatespecificallyreactswithanenzymetogenerateaproduct,whichinteractswithlactateprobetoproducecolor(atλmax=570nm)andflorescence(Ex/Em=535/587nm).ThekitprovidesaconvenientmeansfordetectingL(+)-Lactateinbiologicalsamplessuchasblood,cells,culturemedia,fermentationmedia,etc.Thereisnoneedforpretreatmentorpurificationofsamples.Thekitcandetect0.001-10mMofvariousLactatesamples.

Kit Contains: Lactate Assay Buffer, Lactate Probe, DMSO, Lactate Enzyme Mix, L(+)-LactateStandard,andanInstructionManual.

C F L

D-Lactate Assay Kit

D-LactateAssayKit Cat.#K667-100 100assays

D-Lactateproductioninmammals,mainlyduetotheglyoxalasepathway,isextremelylow,withnormalserumconcentrations inthenanotomicromolar range.Typically,elevatedD-lactatelevelswhichcanrisetomillimolarlevels,areduetobacterialinfectionorshortbowelsyndromeinhumans.AbnormallyhighconcentrationsofD-lactateareconsideredindicativeofsepsis,ischemiaortrauma.Duetoslowmetabolismandexcretion,highD-lactatecancauseacidosisandencephalopathy.BioVision’sD-LactateAssayKitprovidesafast,easywaytoaccuratelymeasureD-lactateinavarietyofbiologicalsamples.IntheD-Lactate Assay Kit, D-lactate is specifically oxidized by D-lactate dehydrogenase andgeneratesproportionalcolor(λmax=450nm).ThekitdetectsD-Lactateinsamplessuchasserum,plasma,cells,cultureandfermentationmedia.Theusefulconcentrationrangeinsamplesis0.01mM–10mMD-lactate.

Kit Contains:D-LactateAssayBuffer,D-LactateEnzymeMix,D-LactateSubstrateMix,D-LactateStandardandanInstructionManual.

y = 0.0602x

0

0.1

0.2

0.3

0.4

0.5

0.6

0 2 4 6 8 10

OD

450

nm

D Lactate (nmol)

L Lactate

C F L

C F L



0

0.4

0.8

1.2

1.6

2

10000 20000 40000

Jurkat Cells (Cell Numbers/Well)

OD

495n

m

LDH Quantification Kit

LDH-Cytotoxicity Assay Kit

LDHQuantificationKit Cat.#K726-500 500assays

LDH-CytotoxicityAssayKit Cat.#K311-400 400assays

Lactatedehydrogenase(LDH)isanoxidoreductasepresentinawidevarietyoforganisms.ItcatalysestheinterconversionofpyruvateandlactatewithconcomitantinterconversionofNADHandNAD+.Whendiseaseorinjuryortoxicmaterialdamagestissues,cellsreleaseLDHintothebloodstream.SinceLDHisafairlystableenzyme,LDHhasbeenwidelyusedtoevaluatethepresenceofdamageandtoxicityoftissueandcells.QuantificationofLDHhasbroadrangeofapplications.InthiscolorimetricLDHquantificationassay,LDHreducesNADtoNADH,which then interactswithaspecificprobe toproduceacolor (λmax=450nm).ThekitquantifiesLDHactivityinavarietyofbiologicalsamplessuchasinserumorplasma,cells,culturemediumandfermentation,etc.Theassayisquick,convenient,andsensitive.Thekitcandetect1-100mIU/mlofLDHdirectlyinsamples

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membranedamage.TheLDH-CytotoxicityAssayKitprovidesa fastandsimplemethod forquantitatingcytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH), a stablecytoplasmicenzymepresentinallcellsandrapidlyreleasedintothecellculturesupernatantupondamageoftheplasmamembrane.LDHactivitycanbedeterminedbyacoupledenzymaticreaction:LDHoxidizeslactatetopyruvatewhichthenreactswithtetrazoliumsaltINTtoformformazan.The increase intheamountof formazanproduced inculturesupernatantdirectlycorrelatestotheincreaseinthenumberoflysedcells.Theformazandyeiswater-solubleandcan be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive,convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes~0.5–1hr.

Kit Contains:LDHAssayBuffer,LDHSubstrateMix,NADHStandard,LDHPositiveControlandanInstructionManual.

Kit Contains:Catalyst,DyeSolutionandanInstructionManual.

y = 0.0761x + 0.0226R² = 0.9981

0

0.5

1

0 5 10

OD45

0


NADH Standard Curve

0

0.5

1

0 10 20 30

OD45

0

Time (min)

Time Curve

PositiveControlSerumSampleBacKGround

y = 0.0761x + 0.0226R² = 0.9981

0

0.5

1

0 5 10

OD45

0


NADH Standard Curve

0

0.5

1

0 10 20 30

OD45

0

Time (min)

Time Curve

PositiveControlSerumSampleBacKGround

Green bar: Low controlRed bar: High control

Lactose Assay Kit

LactoseAssayKit Cat.#K624-100 100assays

0

0.4

0.8

1.2

1.6

0 2 4 6 8 10

Galactose Lactose Lactose w/o Lactase

OD570nm

(Ga)Lactose Amount (nmol/well)

Lactosemakesuparound2-8%ofmilk. Itconsistsofonegalactoseandoneglucose.Somepeople, particularly infants, lack the enzyme necessary to digest galactose leading togalactoseaccumulationinblood(Galactosemia)causingenlargedliver,renalfailure,cataractsandbraindamage.TheLactoseAssayKitprovidesasimple,convenient,andsensitivemeansfordirectmeasurementoflactoselevelsinvariousbiologicalsamples(serum,plasma,otherbodyfluids,food,growthmedia,etc.).IntheAssay,Lactasespecificallyhydrolyzeslactosetogalactoseandglucose.ThegalactoseisspecificallyoxidizedtoproduceaproductthatreactswithaProbetogeneratecolor(OD=570nm)andfluorescence(Ex/Em=535/587nm).Freegalactosebackgroundcanbeeliminatedbyabackgroundcontrolintheabsenceoflactase..

Kit Contains:LactoseAssayBuffer,LactoseProbe,Dimethylsulfoxide,Lactase,LactoseEnzymeMix,LactoseStandard,andanInstructionManual.

C F L

C F L

C F L



LDH-Cytotoxicity Assay Kit II

Lipase Assay Kit

LDH-CytotoxicityAssayKitII Cat.#K313-500 500assays

LipaseAssayKit Cat.#K722-100 100assays

Lactatedehydragenase(LDH)isastableenzyme,presentinallcelltypes,andrapidlyreleasedintothecellculturemediumupondamageoftheplasmamembrane.LDH,therefore, isthemostwidelyusedmarkerincytotoxicitystudy.BioVision’sLDHCytotoxicityAssayKitIIutilizestheadvancedWSTreagentforafastandsensitivedetectionofLDHreleasedfromdamagedcells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generateNADH,whichthenreactswithWSTtogenerateyellowcolor.Usingthehighlysensitiveassay,cells can be cultured in regular 10% serum containing medium for the assay. In addition,since the WST is more stable, the reaction can be read multiple times, or stopped at anytimepointduringthereaction.LDHactivitycanbeeasilyquantifiedbyspectrophotometerorplatereaderatOD=450nm.ThekitprovidesallnecessaryreagentsincludingLDHpositivecontrol.Assaytakeslessthan1hour.

Lipase isasolubleenzymethatcatalyzes thehydrolysisofesterbonds inwater–insoluble,lipidsubstrates.Itplaysessentialrolesinthedigestion,transportandprocessingofdietarylipids (e.g. triglycerides, fats, oils) in most living organisms. Most lipases act at a specificposition on the glycerol backbone of lipid substrate (A1, A2 or A3). For example, humanpancreaticlipase(HPL),whichisthemainenzymetobreakdownfatsinthehumandigestivesystem, converts triglyceride substrates found in ingested oil to monoglycerides and freefatty acids. Studies have shown that Lipases are involved in diverse biological processesrangingfromroutinemetabolismofdietarytriglyceridestocellsignalingandinflammation.BioVision’s Lipase Activity Assay Kit provides a convenient means for determining lipaseactivityinavarietyofbiologicalsamples.Theassayissimple,sensitive,andreliable.Resultscanbeanalyzedbybothfluorometricandcolorimetricmethods.

Kit Contains: WST Substrate Mix, LDH Assay Buffer, Cell Lysis Solution, Stop Solution,LDHandanInstructionManual.

Kit Contains:LipaseAssayBuffer,OxiRedTMProbe,DMSO,EnzymeMix,LipaseSubstrate,LipasePositiveControlandanInstructionManual.

Magnesium Assay Kit

MagnesiumAssayKit Cat.#K385-100 100assays

Magnesiumisthe11thmostabundantelementbymassinthehumanbody.Mg2+isessential to all living cells where it plays an important role in facilitating theprocessing of biological polyphosphates like ATP, DNA, RNA and enzyme functions.Mg2+compoundsareusedaslaxatives,antacids,andusedtostabilizeabnormalnerveexcitationandbloodvesselspasmi.e.,eclampsia.TheBioVisionMagnesiumAssayKitprovidesasimplesensitivemeansofquantitatingmagnesiuminavarietyofbiologicalsamples. The kit takes advantage of the specific requirement of glycerol kinase forMg2+.Anenzymelinkedreactionleadstoformationofanintenselycolored(λmax=450nm)productwhoseformationisproportionaltoMg2+concentration.Thelinearrangeoftheassayis2-15nmoleswithdetectionsensitivity~40μM.

Kit Contains:MagnesiumAssayBuffer,MagnesiumDeveloper,MagnesiumEnzymeMix,MagnesiumStandardandanInstructionManual.

y = 2.0083x + 3.4422y = 0.0553x + 0.054

0

0.2

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0.6

0.8

1

0

10

20

30

40

0 3 6 9 12 15

OD450

nm

Slop

e

nmol Magnesium

Green bar: INT MethodRed bar: WST-1 Method

Magnesium (nmol)

C F L

C F L

C F L



Malate Assay Kit

MalateAssayKit Cat.#K637-100 100assays

L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycleduring carbon fixation in plants. In lower organisms, malate is converted to lactateduring malolactic fermentation with the formation of CO2. Malate is frequently usedas an additive in the food and pharmaceutical industries, so quantitating malic acid isimportant inmanufacturingbeer,wine,cheeseandfruits,amongothers.BioVisionhasdeveloped an easy and sensitive assay to measure the L(-) Malate level in a variety ofsamples.Intheassay,malateisspecificallyoxidizedtogenerateaproductwhichreactswith a substrate probe to generate color (λmax = 450 nm). The assay can detect 1 ~ 35nmolofMalateina50μlsamplewithadetectionsensitivity~20μM.

y = 0.0483x + 0.011

00.20.40.60.81

1.2

0 5 10 15 20 25

O.D.450

nmol/well

Malate

Kit Contains:MalateAssayBuffer,MalateEnzymeMix,WSTSubstrate,MalateStandard,andanInstructionManual.

MaltoseandGlucoseAssayKit Cat.#K618-100 100assays

Maltose and Glucose Assay KitGlucoseandMaltosearethemainfuelsourcestogeneratetheuniversalenergymoleculeATP. Maltose is the major disaccharide generated from hydrolysis of starch in food.Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can beeasilyconvertedtotwoglucosesbyα-D-glucosidase.Glucoseoxidasespecificallyoxidizesfreeglucosetoproduceaproductthatinteractswiththeglucoseprobetogeneratecolorandfluorescence.Therefore,glucoseormaltoselevelsinvariousbiologicalsamples(e.g.serum, plasma, body fluids, food, growth medium, etc.) can be easily determined byeithercolorimetric(spectrophotometryatλ=570nm)orfluorometric(Ex/Em=535/587nm)methods.Thismethodcandetect10pmolto10nmolglucoseperassay.

Kit Contains:GlucoseAssayBuffer,GlucoseProbe,Dimethylsulfoxide,α-D-Glucosidase,GlucoseEnzymeMix,MaltoseStandardandanInstructionManual.

0

0.6

1.2

1.8

0 1 2 3 4 5Maltose (nmol/Well)

O.D

. 570

nm

Maltose Assay Kit

MaltoseAssayKit Cat.#K628-100 100assays

Maltose,oneofthemainfuelsourcestogeneratetheuniversalenergymoleculeATP,isthemajordisaccharidethatisgeneratedfromhydrolysisofstarchinfood.Maltosecontainstwoglucoseunits joinedbyaα-1,4-glycosidic linkage,whichcanbeeasilyconvertedtotwoglucosesbyα-D-glucosidase.Thegeneratedglucosecanbespecificallyoxidized toproduceaproductthatinteractswiththeprobetogeneratecolorandfluorescence.Thus,maltose can be determined by either colorimetric (at λmax = 570 nm) or fluorometric(Ex/Em=535/587nm)methods.Thekitprovidesa fast,easyandsensitivemethodforquantifyingmaltoseinvariousbiologicalsamples(e.g.serum,plasma,bodyfluids,food,growthmedium,etc.).Thedetectionlimitis2μm.

0

0.6

1.2

1.8

0 1 2 3 4 5Maltose (nmol/Well)

O.D

. 570

nm

Kit Contains:MaltoseAssayBuffer,MaltoseProbe,Dimethylsulfoxide,α-D-Glucosidase,EnzymeMix,MaltoseStandard,andanInstructionManual.

C F L

C F L

C F L



NAD/NADH Quantification Kit

NADP/NADPH Quantification Kit

NAD/NADHQuantificationKit Cat.#K337-100 100assays

NADP/NADPHQuantificationKit Cat.#K347-100 100assays

1.

0.00

0.40

0.80

1.20

1.60

0 20 40 60 80 100

O. D

. 450

nm

NAD, NADP, NADH (pmol)

NAD

NADH

NADP

Assay of nicotinamide nucleotides is of continual interest in the studies of energytransformingandredoxstatesofcellsandtissues.BioVision’sNADH/NADQuantificationKit provides a convenient tool for sensitive detection of the intracellular nucleotides:NADH,NADandtheirratio.TheNADCyclingEnzymeMixinthekitspecificallyrecognizesNADH/NADinanenzymecyclingreaction,butnotNADPorNADPH.Theenzymecyclingreactionsignificantly increasesthedetectionsensitivityandspecificity,andthere isnorequirementtopurifyNADH/NADfromsamples.NADandNADHcanbeeasilyquantifiedusingplatereaderat450nm.

Assays of nicotinamide nucleotides are of continual interest in the studies of energytransforming and redox states of cells and tissues. The NADP/NADPH Quantification Kitprovidesaconvenienttoolforsensitivedetectionoftheintracellularnucleotides:NADP,NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPHin an enzyme cycling reaction (It does not recognize NAD+/NADH). The enzyme cyclingreactionsignificantlyincreasesdetectionsensitivity,andthus,thereisnoneedtopurifyNADP/NADPHfromsamplemix.Resultscanbequantifiedusingplatereaderatλmax=450nm.

0.000

0.300

0.600

0.900

1.200

1.500

0 20 40 60 80 100

O.D

. 450

nm

Doses of NADH, NADPH (pmol/well)

NADH

NADPH

Kit Contains:NADH/NADExtractionBuffer,NADCyclingBuffer,NADCyclingEnzymeMix,NADHDeveloper,StopSolution,NADHStandard,andanInstructionManual.

Kit Contains:NADP/NADPHExtractionBuffer,ADPCyclingBuffer,NADPCyclingEnzymeMix,NADPHDeveloper,StopSolution,NADPHStandard,andanInstructionManual.

Nickel Assay Kit

NickelAssayKit Cat.#K510-100 100assays

Nickel isoneoffourferromagneticelements.Severalenzymesdependonnickelforactivity,including some ureases, carbon monoxide dehydrogenases and some hydrogenases. Nickelforms complexes with sulfhydryl compounds with significant absorbance in the UV/visibleregioninthepresenceofotherions.BioVision’sNickelAssaykitprovidesasimplemethodofquantitatingNi2+inavarietyofsamples.TheassaytakesadvantageofreactionofNi2+withmercaptoethanolinboratebuffertoformacomplexwithstrongabsorbancebandsfrom~300to 600 nm. Fe2+ and Co2+ interfere with the assay, therefore extra steps as described in theprotocolmustbetakentosubtractthe interference inordertodeterminethecorrectNickelconcentrationinmixedsamples.Otherionstested(Mn2+,Cu2+,Zn2+)donotinterferewiththeassay.TheassayisasimplemethodofquantitatingNi2+inavarietyofsamples,whichgivesalinearrangeof2to50nmolNickelcontaininglessthan25nmolCobalt.

y = 0.0452x + 0.1228y = 0.0161x + 0.0127y = 0.0093x + 0.1246

y = 0.0017x + 0.0411y = 0.0373x + 0.1491

y = 0.0402x + 0.0558

0

0.5

1

1.5

2

2.5

0 10 20 30 40 50

OD

Ni+2 (nmol/well)

Ni 330Ni 405Fe 330Fe 405Co 330Co 405

Kit Contains: Nickel Assay Buffer, Nickel Reagent, Nickel Chloride Standard and anInstructionManual.

C F L

C F L

C F L



Nitric Oxide Colorimetric Assay Kit

Nitric Oxide Fluorometric Assay Kit

NitricOxideColorimetricAssayKit Cat.#K262-200 2x96assays

NitricOxideFluorometricAssayKit Cat.#K252-200 2x96assays

2000

4000

6000

8000

10000

12000

0 200 400 600 800 1000

RFU

Nitrite/Nitrate (pmol per well)

nitrate NRnitritenitrate + NR

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation,immuneresponseandapoptosis.SinceNOisrapidlyoxidizedtonitriteandnitrate,thetotalconcentrationofnitriteandnitrateisusedasaquantitativemeasureofNOproduction.BioVision’sNitricOxideColorimetricAssayKitprovidesanaccurate,convenientmeasureoftotalnitrate/nitriteinasimpletwostepprocess.Thefirststepconvertsnitratetonitriteutilizingnitrate reductase.ThesecondstepusesGriessReagents toconvertnitrite toadeeppurpleazocompound.Theamountoftheazochromophoreaccuratelyreflectsnitricoxide amount in samples. The detection limit of the assay is approximately 0.1 nmolenitrite/well,or1μM.

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation,immune response and apoptosis. Since NO is rapidly converted to nitrite (NO2-) andnitrate (NO3-), the total concentration of nitrite and nitrate is used as a quantitativemeasure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides anaccurateandconvenientmeasurementof totalnitrate/nitriteconcentration inasimpletwo-stepprocess.Inthefirststepnitrateisconvertedtonitritebynitratereductase.Inthesecondstep,nitritereactswiththefluorescentprobeDAN(2,3diaminonaphthalene).NaOHenhancesthefluorescentyield.Thefluorescentintensityisproportionaltothetotalnitricoxideproduction.Thekithasbeentestedwithculturemedia,plasma,andtissuehomogenates.Thelowerdetectionlimitis~50nm.

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 2 4 6 8 10

OD54

0

nmoles nitrate (nitrite)Kit Contains: Assay Buffer, Enzyme cofactor, Enhancer, Nitrate Reductase, NitrateStandard,NitriteStandard,GriessReagentR1,GriessReagentR2,MicrotiterPlate,PlateCover,andanInstructionManual.

Kit Contains: Assay Buffer, Enzyme Cofactor, Enhancer, Nitrate Reductase, NitrateStandard,NitriteStandard,DANProbe,SodiumHydroxide,Microtiterplate,PlateCover,andanInstructionManual.

Oxaloacetate Assay Kit

OxaloacetateAssayKit Cat.#K659-100 100assays

Oxaloacetate(OAA,HOOC-CO-CH2-COOH)isaTCAcycleintermediate.Itprecedescitratewhich is formed by the transfer of an acetyl group to OAA. OAA is formed by thedeamidation of aspartate or condensation of CO2with pyruvate or PEP. Since mammalsdo not possess the enzymatic machinery to form TCA cycle intermediates from acetylCoA,OAAisoneoftheanapleroticentrypointsviapyruvateandpyruvatecarboxykinase.BioVision’s Oxaloacetate Assay Kit provides a simple, sensitive and rapid means ofquantifyingOAAinavarietyofsamples.Intheassay,OAAisconvertedtopyruvatewhichisutilizedtoconvertanearlycolorlessprobetoanintenselycolored(λmax=570nm)andfluorescent(Ex/Em=535/587nm)product.TheOxaloacetateAssayKitcandetect0.1-10nmol(2-200μM)ofOAA.

y = 0.1251x + 0.0208

00.20.40.60.81

1.2

0 2 4 6 8 10

OD57

0nm

OAA (nmol)/well

Kit Contains: OAA Assay Buffer, OAA Probe, DMSO, OAA Enzyme Mix, Developer, OAAStandardandanInstructionManual.

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Phenylalanine Fluorometric Assay Kit

Phenylalaninefluorometricassaykit Cat.#K572-100 100assays

L-Phenylalanine(PHE)isanelectrically-neutralaminoacid,oneofthetwentycommonand one of the three aromatic amino acids used to biochemically form proteins.Phenylalanineusesthesameactivetransportchannelastryptophantocrosstheblood-brainbarrier,and,inlargequantities,interfereswiththeproductionofserotonin.Errorsin PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.BioVision’s Phenylalanine Assay Kit provides a quick, simple, accurate method forquantifyingPHEinbiologicalsamples.Intheassay,PHEisreductivelydeaminatedwiththesimultaneousformationofNADHwhichreactswithourfluorescentprobetogeneratefluorescenceatEx/Em=535/587nm.Theassayislinearintherangefrom0.1to1.0nmol(2-20mM)ofPhenylalanine.

y = 2043.5x + 13.267

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RFU587nm

Phenylalanine (nmol) per assay

Kit Contains: Phenylalanine Assay Buffer, Tyrosinase, Enzyme Mix, Developer,PhenyalanineStandard,andanInstructionManual.

Phosphate Colorimetric Assay Kit

Phosphate Fluorometric Assay Kit

PhosphateColorimetricAssayKit Cat.#K410-500 500assays

PhosphateFluorometricAssayKit Cat.#K420-100 100assays

y = 0.2985x 0.001

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Phosphateisoneofthemostimportantinorganicionsinbiologicalsystems.Itfunctionsasamolecularswitch,turningenzymeactivityonandoffthroughthemediationofthevarious protein kinases and phosphatases in biological systems. The newly designedPhosphate Colorimetric Assay Kit provides an easy, quick and sensitive means ofassessingphosphateoverawiderangeofconcentrations.Theassayutilizesaproprietaryformulationofmalachitegreenandammoniummolybdatewhich formsachromogeniccomplex with phosphate ion giving an intense absorption band around OD = 650 nm.Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detectionofapproximately0.1nmol,canbedirectlydetermined.ThePhosphateColorimetricAssayKitprovides500assaysusingmicrotiterplatesor100assaysusing1mlcuvettes.

Inorganic phosphate (Pi) is one of the most important ions in biological systems.It functions as a molecular switch, turning enzyme activity on and off through themediation of the various protein kinases and phosphatases in biological systems. ThenewlydesignedPhosphateFluorometricAssayKitprovidesahighlysensitive,easy,quickmeansofassessingphosphateoverawiderangeofconcentrations.Intheassay,phosphateresults inglucoseproduction.TheglucoseisspecificallyoxidizedtogenerateaproductthatreactswiththeOxiRed™probetogeneratefluorescence(Ex/Em=535/587nm).ThekitcanbeusedtodetectPi inavarietyofsamples.Phosphateconcentrationsbetween0.4μMand10μM,withalowerdetectionlimitofapproximately40pmol,canbedirectlydetermined.

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Phosphate (pmole)

Phosphate Standard Curve 2 hours

Kit Contains: Assay Buffer, OxiRed™ probe, Dimethylsulfoxide, Maltose Converter,GlucoseDeveloper,Substrate,PhosphateStandard,andanInstructionManual.

Kit Contains:PhosphateReagent,PhosphateStandard,andanInstructionManual.

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Phosphatidylcholine Quantification Kit

PLTP Activity Assay Kit

PLTP Drug Screening Kit

PhosphatidylcholineQuantificationKit Cat.#K576-100 100assays

PLTPActivityAssayKit Cat.#K604-100 100assays

PLTPDrugScreeningKit Cat.#K605-100 100assays

Plasmaphospholipidtransferprotein(PLTP)isthoughttoplayamajorroleinthefacilitatedtransfer of phospholipids between lipoproteins and in the modulation of high densitylipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activityis related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity,and insulin resistance. The PLTP Activity Assay Kit uses a donor molecule containing afluorescentself-quenchedphospholipidthatistransferredtoanacceptormoleculeinthepresenceofPLTP.PLTP-mediatedtransferofthefluorescentphospholipidtotheacceptormoleculeresultsinanincreaseinfluorescence(Ex/Em=465/535nm).

Plasmaphospholipidtransferprotein(PLTP)playsamajorroleinthefacilitatedtransferof phospholipids between lipoproteins. PLTP-facilitated lipid transfer activity is relatedtoHDLandLDLmetabolism,aswellaslipoproteinlipaseactivity,adiposity,andinsulinresistance.ThePLTPDrugScreeningKitutilizesrabbitserumasthePLTPactivitysourcetoscreenPLTPinhibitors.Theassayusesadonormoleculecontainingafluorescentself-quenched phospholipid that is transferred to an acceptor molecule in the presence ofPLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor moleculeresultsinanincreaseinfluorescence(Ex/Em=465/535nm).InhibitorofPLTPwillinhibitthelipidtransferandthereforedecreasefluorescenceintensity.Thekitprovidessufficientreagentsfor100PLTPinhibitorscreeningassays.

y = 0.1563x 0.002

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PC (nmol)

Kit Contains:PCAssayBuffer,OxiRed™Probe,DMSO,Anhydrous,PCHydrolysisEnzyme,PCDevelopmentMix,PCStandard(10μmol),andanInstructionManual.

Kit Contains: PLTP Donor Molecule, PLTP Acceptor Molecule, PLTP Assay Buffer (10X),PositiveControl(RabbitSerum),andanInstructionManual.

Phosphatidylcholine(PC)isaphospholipidwhichincorporatescholineastheheadgroupof the lipid. PC is a major constituent of biological membranes and is involved in cellsignaling through release of choline by phospholipase D leaving the second messengerphosphatidic acid. BioVision’s Phosphatidylcholine Assay Kit is a simple and convenientmeans of measuring Phosphatidylcholine in a variety of biological samples. The kitutilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline whichis subsequently oxidized resulting in development of the OxiRed™ probe to generatefluorescence (Ex/Em = 535/587 nm) and absorbance (OD = 570 nm). The kit measuresPC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM(~50-200mg/dL).

y = 9504.9x 126.82

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PC (nmol)

Kit Contains:PCAssayBuffer,OxiRed™Probe,DMSO,Anhydrous,PCHydrolysisEnzyme,PCDevelopmentMix,PCStandard,andanInstructionManual.

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Pyruvate Assay Kit

Pyruvate Kinase Assay Kit

Sarcosine Assay Kit

PyruvateAssayKit Cat.#K609-100 100assays

PyruvateKinaseAssayKit Cat.#K709-100 100assays

SarcosineAssayKit Cat.#K636-100 100assays

Pyruvate is a central molecule in metabolism through which sugars enter the citricacid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or tofatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease andgeneticdisorders.Pyruvatehasalsobeenusedtostimulatemetabolismleadingto lossofbodyweight.BioVisionprovidesasimple,directandautomation-readyprocedureformeasuring pyruvate concentration in various biological samples such as blood, cells,cultureandfermentationmedia,etc.Intheassay,pyruvateisoxidizedbypyruvateoxidasevia enzyme reactions to generate color (at λmax = 570 nm) and fluorescence (at Ex/Em= 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvatecontent, the pyruvate concentration can be accurately measured. The kit detects 1-10000μMpyruvate.

Pyruvate kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of aphosphate group from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP.Lackofpyruvatekinasewillslowdowntheprocessofglycolysiswhichcausesthediseaseknownaspyruvatekinasedeficiency.BioVisionprovidesasimple,directandautomation-readyprocedureformeasuringpyruvatekinaseactivityinvariousbiologicalsamplessuchas blood, tissues, and culture cells, etc. In the assay, PEP and ADP are catalyzed by PKto generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidaseto produce color (at λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm), thus, PKactivitycanbeaccuratelymeasured.Thekitdetects0.1mU/mlpyruvatekinase.

Sarcosine,anaturalaminoacid,playsimportantrolesasintermediateinthemetabolismofcholine,methionine,glycine,glutathione,creatine,purineandserine,etc.Detectionofsarcosinelevelhaswideapplicationsinresearchanddevelopment.BioVision’sSarcosineAssayKitprovidesanaccurate,convenientmeasureofsarcosineinavarietyofbiologicalsamples.Intheassay,sarcosineisspecificallyoxidizedtogenerateaproductthatconvertsacolorlessprobetoaproductwith intense redcolor (λmax=570nm)andwhich isalsohighlyfluorescent(Ex/Em=538/587nm).Sarcosineisthereforeeasilydetectedbyeithercolorimetricorfluorometricmethodswithdetectionrange1–10000μM.

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Pyruvate Amount (nmol/well)

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Sarcosine (nmol)

Kit Contains: Pyruvate Assay Buffer, Pyruvate Probe, DMSO, Pyruvate Enzyme Mix,PyruvateStandard,andanInstructionManual.

Kit Contains: Sarcosine Assay Buffer, Sarcosine Probe, DMSO, Sarcosine Enzyme mix,SarcosineStandard,andanInstructionManual.

Kit Contains:PKAssayBuffer,OxiRed™Probe,DMSO,PKEnzymeMix,PKSubstrateMix,PKPositiveControl,PyruvateStandard,andanInstructionManual.

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PK samples time line1.0mU

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0.6mU

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y = 0.1139x + 0.016R² = 0.9988

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Pyruvate (nmol)

Pyruvate Standard Curve

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Sialic Acid (NANA) Assay Kit

SialicAcid(NANA)AssayKit Cat.#K566-100 100assays

Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid,and also named N-acetylneuraminic acid. Sialic acids are found widely distributed inanimaltissues.Studyshowsthatsialicacid levelmaybeassociatedwithdevelopmentalandpathologicalstages.BioVision’sSialicAcidAssayKitprovidesasimpleandconvenientmeansofmeasuringfreeSialicAcidinavarietyofbiologicalsamples.Thekitutilizesanenzyme coupled reaction in which free sialic acid is oxidized resulting in developmentof theOxi-Redprobetogive fluorescence (Ex/Em = 535/587 nm) and absorbance (OD= 570 nm). The kit measures sialic acid in the linear range of 0.1 to 10 nmol with adetectionsensitivity~1μMconcentration.

Kit Contains: Sialic Acid Assay Buffer, Sialic Acid Probe, DMSO, Anhydrous, Sialic AcidConvertingEnzyme,SialicAcidDevelopmentMix,SialicAcidStandard,andanInstructionManual.

y = 8912.7x + 78.371

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nmol Sialic Acid

y = 0.1393x 0.003

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m

nmol Sialic Acid

SOD Activity Assay Kit

SODActivityAssayKit Cat.#K335-100 100assays

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Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. Itcatalyzesthedismutationofthesuperoxideanionintohydrogenperoxideandmolecularoxygen.ThesensitiveSODassaykitutilizesWST-1thatproducesawater-solubleformazandyeuponreductionwithsuperoxideanion.Therateofthereductionwithasuperoxideanion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD.Therefore,theinhibitionactivityofSODcanbedeterminedbyacolorimetricmethod.

Kit Contains:WSTSolution,SODEnzymeSolution,SODAssayBuffer,SODDilutionBufferandanInstructionManual.

Starch Assay Kit

StarchAssayKit Cat.#K647-100 100assays

y = 0.651x 0.027y = 0.700x 0.006

y = 0.653x 0.003y = 0.648x 0.012

y = 0.690x 0.002

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570nm

Starch ( g/well)

glucosecornpotatoricewheat

Starchisacomplexcarbohydrateconsistingofalargenumberofglucoseunits.Allplantscontainstarch,presentasamylose,(linearα-1,4linkedpolymer)andamylopectin,(highlyα-1,6branchedα-1,4polymer).Starchgenerallycontains0-25%amyloseand75–100%amylopectin.TheStarchAssayKitprovidesaneasy,convenientassaytomeasurestarchlevelsinavarietyofsamples.Intheassay,starchishydrolyzedtoglucosewhichisoxidizedtogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Theassaycandetectstarchat0.0004to2mg/ml.

Kit Contains:HydrolysisBuffer,DevelopmentBuffer,OxiRed™Probe,DMSO,HydrolysisEnzymeMix,DevelopmentEnzymeMix,StarchStandard,andanInstructionManual.

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Antioxidant (1 nmol)

Sucrose Assay Kit

SucroseAssayKit Cat.#K626-100 100assays

Sucrose,alsocalledtablesugar,isoneofthemostimportantfuelsourcestogeneratetheuniversalenergymoleculeATP.Sucroseisadisaccharidewhichcanbeconvertedintooneglucoseandonefructose.BioVision’sSucroseAssayKitprovidesaconvenientmeansforspecificallymeasuringsucroselevelsfromvariousbiologicalsamples(e.g.serum,plasma,bodyfluids,food,growthmedium,etc.).Intheassay,Sucroseisconvertedtoglucoseandfructoseby invertase.Thegeneratedglucose is thenspecificallyoxidizedtogenerateaproduct which reacts with a probe to generate color (λmax = 570 nm) and fluorescence(Ex/Em=535/587nm).Freeglucosebackgroundcanbecorrectedwithpropercontrols.Other disaccharides such as lactose and maltose do not interfere with the assay. Themethodcandetect0.0002-10mMsucroseconcentrations.

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Kit Contains:SucroseAssayBuffer,OxiRedProbe,Dimethylsulfoxide,Invertase,EnzymeMix,SucroseStandard,andanInstructionManual.

Thioredoxin Reductase Assay Kit

ThioredoxinReductaseAssayKit Cat.#K763-100 100assays

Thioredoxinreductase(TrxR)isanubiquitousenzymewhichisinvolvedinmanycellularprocesses such as cell growth, p53 activity, and protection against oxidation stress,etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substratessuch as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. BioVision’sThioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detectingTrxRactivityinvarioussamples.IntheassayTrxRcatalyzesthereductionof5,5’-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), whichgeneratesastrongyellowcolor(λmax=405nm).Sinceincrudebiologicalsamplesotherenzymes, such as glutathione reductase and glutathione peroxidase, can also reduceDTNB,aTrxRspecificinhibitorisutilizedtodetermineTrxRspecificactivity.Thedetectionlimitis~0.2mU.Kit Contains:TrxRAssayBuffer,TNBStandard,DTNB,NADPH,TrxRPositiveControl,TrxRInhibitor,andanInstructionManual.

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mTime (min)

TrxR Kinetic Data2.0 mU

1.6 mU

1.2 mU

0.8 mU

0.4 mU

0 mU

y = 0.036x + 0.0626R² = 0.9992

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m

TNB Amount (nmol)

TNB Standard Curve

Total Antioxidant Capacity (TAC) Assay Kit

TotalAntioxidantCapacity(TAC)AssayKit Cat.#K274-100 100assays

Antioxidants play an important role in preventing the formation of and scavenging offreeradicalsandotherpotentiallytoxicoxidizingspecies.Therearethreecategoriesofantioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), smallmolecules(ascorbate,uricacid,GSH,vitaminE,etc.)andproteins(albumin,transferrin,etc.).BioVision’sTACAssayKitmeasuressmallmoleculeand/orproteinantioxidants. Intheassay,Cu2+ionisconvertedtoCu+bybothsmallmoleculeandprotein.OurproprietaryProteinMaskpreventsCu2+reductionbyprotein,enablingtheanalysisofonlythesmallmoleculeantioxidants.ThereducedCu+ionischelatedwithacolorimetricprobegivingabroadabsorbancepeakaround570nm,proportionaltothetotalantioxidantcapacity.

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Trolox (nmol)

Trolox standard curve

Kit Contains: Cu2+ Reagent, Assay Diluent, Protein Mask, Trolox Standard, and anInstructionManual.

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Triglyceride Assay Kit

TriglycerideAssayKit Cat.#K622-100 100assays

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Triglyceride (nmol)

Triglycerides(TG)arethemainconstituentofvegetableoil,animalfat,LDLandVLDL,andplayanimportantroleastransportersoffattyacidsaswellasservingasanenergysource.TGarebrokendownintofattyacidsandglycerol,afterwhichbothcanserveassubstratesfor energy producing and metabolic pathways. High blood levels of TG are implicatedin atherosclerosis, heart disease and stroke as well as in pancreatitis. The TriglycerideQuantificationKitprovidesasensitive,easyassaytomeasureTGconcentrationinavarietyofsamples.Intheassay,TGareconvertedtofreefattyacidsandglycerol.Theglycerolisthenoxidizedtogenerateaproductwhichreactswiththeprobetogeneratecolor(atλmax=570nm)andfluorescence(Ex/Em=535/587nm).Thekitcandetect2pmol-10nmol(or2~10000μMrange)oftriglycerideinvarioussamples.

Kit Contains: Triglyceride Assay Buffer, Triglyceride Probe, Dimethylsulfoxide, Lipase,TriglycerideEnzymeMix,TriglycerideStandard,andanInstructionManual.

Urea Assay Kit

UreaAssayKit Cat.#K375-100 100assays

Ureaisawasteproductwhichproducedintheliver,dissolvedinblood(inaconcentrationof2.5-7.5mM),andexcretedbythekidneys.However,italsoplaysaveryimportantroleinproteincatabolism,removaloftoxicammoniafromthebody,andthecountercurrentsystem which allows for reabsorption of water and critical ions in the nephrons. Ureadeterminationisveryusefulforthemedicalcliniciantoassesskidneyandotherorgans’function of patients. BioVision’s Urea Assay Kit provides a rapid, simple, sensitive, andreliableassayformeasurementofUrealevelinallkindsofsamplessuchasserum,plasma,andurine.Itisalsosuitableforhigh-throughputassays.IntheassayUreareactswithanenzymeto formaproduct that reactswiththeOxiRed™probetogeneratecolor (λmax=570nm).TheopticaldensityisproportionalwithUreaconcentrationinthesolution.

Kit Contains: Urea Assay Buffer, OxiRed™ Probe, Dimethylsulfoxide, Enzyme Mix(lyophilized),UreaStandard,andInstructionManual

Uric Acid Assay Kit

UricAcidAssayKit Cat.#K608-100 100assays

Uric acid in serum is the end product of purine metabolism, and is cleared throughthe kidney by glomerular filtration. Humans often lack the necessary enzyme calledurate oxidase (Uricase), and therefore abnormally high uric acid concentrations maybe accumulated in blood. Recent evidence shows the close association between serumuratelevelandcardiovascularmorbidityandmortality,especiallyamongpersonsathighcardiovascular risk, including those with hypertension, diabetes and congestive heartfailure.BioVision’sUricAcidAssayKitprovidesaconvenientmeansfordetectinguricacidinbiologicalsamplessuchasserumandurine.Pretreatmentofsamplesisnotrequired.Uricacidlevelscanbemeasuredusingfluorometric(Ex/Em=535/587nm)orcolorimetric(λmax=570nm)methods.

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570

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Standard Uric Acid (nmol/well)

Kit Contains:UricAcidAssayBuffer,UricAcidProbe,DMSO,UricAcidEnzymeMix,UricAcidStandard,andanInstructionManual.

y = 0.1933x + 0.2485R² = 0.9979

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Urea Standard Curve

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XanthineOxidaseAssayKit Cat.#K710-100 100assays

Xanthineoxidase (XO,EC1.17.3.2 ) ispresent inappreciableamounts in the liverandjejunuminhealthyindividuals.However,invariousliverdisorders,XOisreleasedintocirculation.Therefore,determinationofserumXOlevelservesasasensitiveindicatorofacuteliverdamagesuchasjaundice.BioVisionhasdevelopedaneasyandsensitiveassay to determine XO in variety of samples. In the assay, XO oxidizes xanthine tohydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed™ Probe togenerate color (at λmax = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Sincethe color or fluorescence intensity is proportional to XO content, the XO activity canbeaccuratelymeasured.Thekitdetects1-100mUxanthineoxidasein100μlreactionvolume.

Kit Contains:XOAssayBuffer,OxiRed™Probe,DMSO(anhydrous),XOEnzymeMix,XOSubstrateMix,XOPositiveControl,H2O2StandardandanInstructionManual.

y = 0.2306x + 0.0773R² = 0.9945

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H2O2 (nmol)

H2O2 Colorimetric StandardCurve

y=0.2366x+ 0.0419R² =0.999

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Xanthine Oxidase Assay Kit

Companion Products

The 10K Spin Columns are disposable ultrafiltration devices for the concentration ofbiologicalsamples.Maximuminitialsamplevolumeis500µl.Theycanbeusedineitheraswingingbucketor fixedangle rotorsaccepting2.0mlcentrifugetubesatmaximumspeed15000g.Thedeadvolumeis~5-15ml.Theverticalmembranedesignandthinchannelfiltrationchamberminimizesmembranefouling andprovideshighspeedconcentration,evenwithconcentratedsolutions. It isspecifically designed with low internal surface and membrane area in order to achievesuperiorrecoveryfromdilutesolution.

ProteinCo

ncen

tration(OD

280nm

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The presence of protein and various enzyme activities frequently interferes with theanalysisofsmallmoleculesinbiologicalsamples.Manybioassaysrequireremovalofpro-teins from samples prior to analysis. Among the deproteinization protocols developedoverthe lasthalfcentury,perchloricacid(PCA)precipitationhasbeenextensivelyusedinmanydifferentsamplepreparationprocedures,sincenotonlydoesitremovemostoftheproteinpresentbutitalsofunctionstostabilizemanyofthesmallmoleculeanalytes.PCA deproteinization methods have been successfully used in the preparation samplespriortoquantificationofanarrayofsmallmolecules,includingglycogen,ATP,cAMP,glu-tathione,antioxidants,etc..ThenewlydesignedDeproteinizingSamplePreparationKitutilizesaPCAprecipitationmethod,providingauniquetooltopreparesamplesfortheanalysis of a variety of small molecules. Using the kit, proteins are precipitated, excessPCAisremoved,samplesareneutralized.Samplespreparedusingthiskitcanbedirectlyusedinawidevarietyofbioassays.Themethodiseasy,convenient,andcanbeusedforthepreparationofalargenumberofsamples.

Kit Contents:PCA,NeutralizationSolutionandanInstructionManual.

Figure:Deproteinizationofserumsamples:Differentamountsof PCA were used to deproteinize 400 µl of serum followingthekitprotocol.ProteinconcentrationremaininginsolutionOD280 (•) and sample pH (•) was measured. Samples (neatserum; serum filtered through a 10kD MW cutoff; and PCAtreated) were diluted 20 times for convenience of measur-ingODat280nm.AseparatealiquotofthesamePCAtreatedsampleswasmixed1:1withapH7.75assaybufferandthepHofthemixturedetermined.Thefigureshowsthatsample/PCAratioerrorsupto20%canbe tolerated with the resulting assay pH holding within ap-proximately0.5pHunitsofthetargetassaypH.Usinga10kDMW cutoff filter removes approximately 98% of protein andPCA precipitation at a PCA/sample ratio of 1:4 removes ap-proximately95%ofproteinpresent.

Deproteinizing Sample preparation Kit

10K Spin Column

DeproteinizingSamplePreparationKit Cat.#K808-200 200assays10KSpinColumn Cat.#1997-25 25each

Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 ��Tech help:email:[email protected] orcall800.891.9699 or 650-428-0236 orfax:650.428.0336 ��

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Downloadourfull-colorProduct Catalog, flyers & Brochures:

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fractionPREPTm Cell fractionation flyerFeaturingseveralconvenientkitsforisolatingmitochondria,nucleus,membrane,cytosolproteinfractionsfordownstreambioassays.

Cholesterol flyerFeaturingassaysforcholesterol,HDL,LDL,CETP,PLTP,Choline,FattyAcid,etc.

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Cell Proliferation & senescence flyerFeaturingtheeasiestandmostsensitiveassays formeasuringcellproliferation,cytotoxicityandsenescence.

cAmP & cgmP flyerFeaturingthemostsensitiveandreliablecAMP&cGMPassaykitsonthemarket.

Annexin v flyerFeaturinghighquality,multi-colorAnnexinVproductsfordetectingapoptosis.

Adipokine flyerFeaturesadiponectin,resistin,leptin,visfatin,etc.

Apoptosis Product overview flyerAcomprehensiveoverviewofvariousapoptosisproductsfordetectingapoptosisatvariousstagesandatdifferentareasofthecell.

Recombinant Protein flyerFeaturing high quality recombinant growth factors, cytokines, chemokines,enzymes,etc.

fmK & QvD-oPh inhibitors flyerThese high quality inhibitors are potent, cell-permeable, non-toxic, andirreversiblybindtoactivatedcaspasestoblockapoptosis.

Antibody flyerFeaturingmanyhighqualitymonoclonalandpolyclonalantibodies.

Cell-based assay flyerFeatures many novel assays for metabolisms, cell signaling, cell fractionation,molecularbiology,cholesterolandobesity,etc.

2008-09 Product Catalog Youcanviewonscreen,savetodiskorprintrightonyourdesktopprinter!

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10K Spin Column . . . . . . . . . . . . . . . . . . . . . . . . . .39 - A -

Pico ProbeTM Acetyl-CoA Assay Kit . . . . . . . . . . . . . . . . 6 Acid Phosphatase Colorimetric Assay Kit . . . . . . . . . . . . 6 Acid Phosphatase Fluorometric Assay Kit . . . . . . . . . . . . 6 Aconitase Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 7 Adipogenesis Assay Kit . . . . . . . . . . . . . . . . . . . . . . 7 Adipolysis Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 7 ADP Colorimetric/Fluorometric Assay Kit . . . . . . . . . . . . 8 ADP/ATP Ratio Assay Kit . . . . . . . . . . . . . . . . . . . . . . 8 Alanine Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 8 Alkaline Phosphatase Colorimetric Assay Kit . . . . . . . . . . 9 Alkaline Phosphatase Fluorometric Assay Kit . . . . . . . . . 9 L-Amino Acid Quantification Kit . . . . . . . . . . . . . . . . . 9 Ammonia Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 10 Amylase Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 10 Ascorbic Acid Assay Kit . . . . . . . . . . . . . . . . . . . . . . 10 Ascorbic Acid Assay Kit II (FRASC) . . . . . . . . . . . . . . . . 11 ATP Cell Viability Assay Kit . . . . . . . . . . . . . . . . . . . . 11 ATP Colorimetric/Fluorometric Assay Kit . . . . . . . . . . . . 11

- B - Branched Chain Amino Acid Assay Kit . . . . . . . . . . . . . . 12

- C - Calcium Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 12 Catalase Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 12 L-Carnitine Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 13 CETP Activity Assay Kit . . . . . . . . . . . . . . . . . . . . . . 13 CETP Inhibitor Drug Screening Kit . . . . . . . . . . . . . . . . 13 Chloride Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 14 Cholesterol Quantification Kit . . . . . . . . . . . . . . . . . . 14 Choline/Acetylcholine Quantification Kit . . . . . . . . . . . . 14 Citrate Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Cobalt Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Coenzyme A (CoA) Assay Kit . . . . . . . . . . . . . . . . . . . 15 Creatine Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 16 Creatinine Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 16

- D - Deproteinizing Sample Preparation Kit . . . . . . . . . . . .39

- E - Ethanol Assay Kit. . . . . . . . . . . . . . . . . . . . . . . . . . 16

- F - FAD Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Free Fatty Acid Quantification Kit . . . . . . . . . . . . . . . . 17 Free Glycerol Assay Kit . . . . . . . . . . . . . . . . . . . . . . 17 Fructose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 18

- G - Galactose and Lactose Assay Kit . . . . . . . . . . . . . . . . . 18 Galactose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . 18 Glucose and Sucrose Assay Kit . . . . . . . . . . . . . . . . . . 19 Glucose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 19 Glucose-6-Phosphate Assay Kit . . . . . . . . . . . . . . . . . 19 Glucose-6-Phosphate Dehydrogenase Assay Kit . . . . . . . .20 Glutamate Assay Kit . . . . . . . . . . . . . . . . . . . . . . .20 Glutathione (GSH/GSSG/Total) Assay Kit . . . . . . . . . . . .20 Glutathione Colorimetric Detection Kit . . . . . . . . . . . . . 21 Glutathione Fluorometric Detection Kit . . . . . . . . . . . . . 21 Glutathione Peroxidase Assay Kit . . . . . . . . . . . . . . . . 21 Glutathione Reductase Assay Kit . . . . . . . . . . . . . . . .22 Glycogen Assay Kit . . . . . . . . . . . . . . . . . . . . . . . .22 GST Colorimetric Assay Kit . . . . . . . . . . . . . . . . . . . .22 GST Fluorometric Assay Kit . . . . . . . . . . . . . . . . . . . .23

- H - HAT Activity Assay Kit . . . . . . . . . . . . . . . . . . . . . . .23 HDAC Colorimetric Assay Kit . . . . . . . . . . . . . . . . . . .23

HDAC Fluorometric Assay Kit . . . . . . . . . . . . . . . . . . 24 HDAC Inhibitor Drug Screening Kit . . . . . . . . . . . . . . . 24 HDL and LDL/VLDL Quantification Kit . . . . . . . . . . . . . . 24 Heme Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . .25 Hydrogen Peroxide Assay Kit . . . . . . . . . . . . . . . . . .25 ß-Hydroxybutyrate (ß-HB) Assay Kit . . . . . . . . . . . . . .25

- I - Iron Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Isocitrate Assay Kit . . . . . . . . . . . . . . . . . . . . . . . .26

- K - a-Ketoglutarate Assay Kit . . . . . . . . . . . . . . . . . . . .26

- L - Lactate Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .27 Lactate Assay Kit II . . . . . . . . . . . . . . . . . . . . . . . .27 D-Lactate Colorimetric Assay Kit . . . . . . . . . . . . . . . . .27 Lactose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .28 LDH Quantification Kit . . . . . . . . . . . . . . . . . . . . . . .28 LDH-Cytotoxicity Assay Kit . . . . . . . . . . . . . . . . . . . .28 LDH-Cytotoxicity Assay Kit II . . . . . . . . . . . . . . . . . . .29 Lipase Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . .29

- M - Magnesium Assay Kit . . . . . . . . . . . . . . . . . . . . . . .29 Malate Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .30 Maltose and Glucose Assay Kit . . . . . . . . . . . . . . . . . .30 Maltose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .30

- N - NAD/NADH Quantification Kit . . . . . . . . . . . . . . . . . . 31 NADP/NADPH Quantification Kit . . . . . . . . . . . . . . . . 31 Nickel Assay Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . 31 Nitric Oxide Colorimetric Assay Kit . . . . . . . . . . . . . . . 32 Nitric Oxide Fluorometric Assay Kit. . . . . . . . . . . . . . . . 32

- O - Oxaloacetate Assay Kit . . . . . . . . . . . . . . . . . . . . . . 32

- P - Phenylalanine Assay Kit . . . . . . . . . . . . . . . . . . . . .33 Phosphate Colorimetric Assay Kit . . . . . . . . . . . . . . . .33 Phosphate Fluorometric Assay Kit . . . . . . . . . . . . . . . .33 Phosphatidylcholine Assay Kit . . . . . . . . . . . . . . . . . .34 PLTP Activity Assay Kit . . . . . . . . . . . . . . . . . . . . . .34 PLTP Inhibitor Drug Screening Kit . . . . . . . . . . . . . . . .34 Pyruvate Assay Kit . . . . . . . . . . . . . . . . . . . . . . . .35 Pyruvate Kinase Assay Kit . . . . . . . . . . . . . . . . . . . . .35

- S - Sarcosine Assay Kit. . . . . . . . . . . . . . . . . . . . . . . . .35 Sialic Acid (NANA) Assay Kit . . . . . . . . . . . . . . . . . . .36 SOD Activity Assay Kit . . . . . . . . . . . . . . . . . . . . . .36 Starch Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .36 Sucrose Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . 37

- T - Thioredoxin Reductase Assay Kit . . . . . . . . . . . . . . . . 37 Total Antioxidant Capacity (TAC) Assay Kit . . . . . . . . . . . 37 Triglyceride Quantification Kit . . . . . . . . . . . . . . . . . .38

- U - Urea Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . .38 Uric Acid Assay Kit . . . . . . . . . . . . . . . . . . . . . . . .38

- X - Xanthine Oxidase Assay Kit . . . . . . . . . . . . . . . . . . . .39

Ordering: call 800.891.9699 or 650.428.0236 or fax: 650.428.0336 or online at www.biovision.com42

Product Index


43Tech Help: email: [email protected] or call 800.891.9699 or 650-428-0236 or fax: 650.428.0336

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Stem Cell Research Tools

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Documents

Metabolism · various metabolism Assay Kits: A Quick glance Tech help: email: [email protected] or call 800.891.9699 or 650-428-0236 or fax: 650.428.0336 C ColorimetricF L Assay