Victoria, BC July 27 – 31, 2019 · Presentation. International 2 3 Gap Junction Conference International Gap Junction Conference #IGJC2019 @int_gap #IGJC2019 WELCOME LETTER FROM

www.igjconference.org

#IGJC2019@int_gap

photo: Tourism Victoria

photo: Tourism Victoria

Victoria, BCJuly 27 – 31, 2019

Victoria Conference Centre

1International Gap Junction Conference www.igjconference.org #IGJC2019

@int_gap

Welcome Letter from Victoria Mayor . . . . . . . . . . . . . . . 2

Welcome Letter from the Co-Chairs . . . . . . . . . . . . . . . 3

About IGJC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

General Conference Information . . . . . . . . . . . . . . . . . 5

Social Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Annual Conference Schedule . . . . . . . . . . . . . . . . . . . 11

Keynote Presentations . . . . . . . . . . . . . . . . . . . . . . 26

Author Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Poster Session . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

IGJC Sponsors . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Poster Floor Plans . . . . . . . . . . . . . . . . . . . . . . . . . 45

TABLE OF CONTENTSPROGRAM AT A GLANCE

8:00 AM8:30 AM8:45 AM9:00 AM9:15 AM9:30 AM9:45 AM10:00 AM10:15 AM10:30 AM10:45 AM11:00 AM11:15 AM11:30 AM11:45 AM12:00 PM12:15 PM12:30 PM12:45 PM1:00 PM1:15 PM1:30 PM1:45 PM2:00 PM2:15 PM2:30 PM2:45 PM3:00 PM3:15 PM3:30 PM3:45 PM4:00 PM4:15 PM4:30 PM4:45 PM5:00 PM5:15 PM5:30 PM5:45 PM6:00 PM6:15 PM6:30 PM6:45 PM7:00 PM7:15 PM7:30 PM7:45 PM8:00 PM8:15 PM8:30 PM8:45 PM9:00 PM9:15 PM9:30 PM9:45 PM10:00 PM

Post

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8:30

-13:

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Session 913:15-14:45

Session 710:00-11:30

Marta Varela-Eirin

Welcome Address

Jean Jiang Keynote08:45-9:30Cherie Brown

Coffee Break

Reg

Des

k O

pen

14:3

0-18

:00

Coffee Break

Session 110:00-11:30

Session 213:15-14:45

Coffee Break

Opening Drink Reception

Grazing Dinner 18:30 - 20:30

Reg

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:00

- 18:

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Brenda Kwak Keynote08:45-9:30

Erin Mulkearns-Hubert

Post

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:30-

18:0

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Session 315:00-16:30

Reg

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00am

-13:

00pm

Reg

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-5:0

0pm

Coffee Break

Closing & Prizes

Coffee Break

Lunch11:30-13:15

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8:30

-14:

45

Lunch11:30-13:00

Optional Day Trips

Rob Gourdie Keynote08:45-9:30

Coffee Break

Session 810:00-11:30

IGJCProgram-At-A-Glance

Saturday Sunday Monday Tuesday Wednesday27-Jul 28-Jul 29-Jul 30-Jul 31-Jul

Conference Dinner19:00-22:00

Session 615:00-16:30

Post

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8:30

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Reg

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:00

- 18:

00Coffee Break

Session 410:00-11:30

Session 513:15-14:45

Doug Bayliss Keynote08:45-9:30

Juan Sanchez-Arias

Poster Session 116:30-18:00

Lunch11:30-13:15

Poster Session 216:30-18:00

Lunch11:30-12:45

Session 1015:00-16:15

2021 Future Conference Presentation

2 3International Gap Junction Conference

International Gap Junction Conferencewww.igjconference.org www.igjconference.org#IGJC2019

@int_gap #IGJC2019@int_gap

WELCOME LETTER FROM VICTORIA MAYORWELCOME LETTER FROM VICTORIA MAYOR

WELCOME LETTER FROM THE CO-CHAIRS

N o . 1 C e n t e n n i a l S q u a r e V i c t o r i a B r i t i sh C o lu mb i a C a n a d a V 8 W 1 P 6

Te l e p h o n e ( 2 5 0 ) 3 6 1 - 0 2 0 0 F a x ( 2 5 0 ) 3 6 1 -0 3 4 8 Em a i l m a yo r @ v i c t o r i a . c a

Greetings from Mayor Lisa Helps

On behalf of the City of Victoria, it is my great pleasure to

welcome you to British Columbia’s Capital City for the

2019 International Gap Junction Conference (IGJC). We

are honoured to host professionals from across the country,

collaborating on the continued good health of our nation.

While you are in Victoria, I hope you will take some time

to explore our beautiful city. Put on your walking shoes

and enjoy the sights and tastes of downtown and terrific

surrounding neighbourhoods and village centres. Enjoy

our picturesque Inner Harbour, Fisherman’s Wharf,

waterfront paths, local shops and some of the best food,

beer and coffee in the country. Make sure to wander through Canada’s oldest

Chinatown, and our charming Old Town district.

I wish you all the best for an enjoyable conference filled with new knowledge,

collaboration and innovation. I hope that your time in Victoria will be so memorable that

we will soon welcome you for a return visit!

Sincerely,

Lisa Helps

Victoria Mayor

Dear IGJC2019 attendees,

As co-chairs of the Executive Organizing Committee we would like to extend to you a warm welcome to Victoria, the exquisite site of this year’s meeting.

Your attendance at this meeting underscores your commitment to the international community of gap junction (and related protein) researchers. These biennial conferences have been held for almost 40 years in various international locations, providing a unique opportunity to present novel findings in various areas of gap junction research.

The sense of community and the exchange of new ideas facilitated by this meeting is critical to the strength and growth of the gap junction field. In participating and supporting this endeavor, we are collectively laying a fertile soil in which to sow the seeds of future growth in our research by our incredible trainees.

Further, to quote the acclaimed Nobel Prize-winning Canadian short story writer, Alice Munro, “The constant happiness is curiosity.” As scientists, we are driven by curiosity, and there is no better way to foster our curiosity than by exchanging new and exciting ideas.

We are extremely privileged to be able to meet together to enable these important exchanges in beautiful Victoria, BC. We’ve designed the program so that you can take advantage of the opportunity to enjoy the beauty and outdoor experiences of Victoria, whether that be by going for a morning run along Dallas Road and through Beacon Hill Park or walking along the Inner Harbour during the extended lunch breaks. Tuesday, we have a half day of sessions and plenty of time to enjoy the things that make Victoria unique! Whale watching for the orcas, flower spotting at the world famous Butchart Gardens, or a city tour to see the famous and infamous Victoria landmarks are just some of the many activities you can partake in.

Make sure to take some time to recharge in the refreshing ocean air between sessions so you are invigorated for the fantastic science that is on offer at IGJC2019.

Enjoy the meeting!

Cordially,

Christian Naus Co-Chair University of British Columbia

Leigh Anne Swayne Co-Chair University of Victoria




ABOUT IGJC

The International Gap Junction Conference is held every two years since 1974, alternating primarily between North America and Europe. The meeting brings together a diverse international community of basic science researchers that focus on connexins and pannexins. The meeting topics include the cell biology/interactomes/signaling/trafficking, channel structure and function/biophysics of connexins and pannexins and their role in growth control, inflammation, cardiovascular function, development/reproduction, inherited diseases, the nervous system and other sensory systems.

IGJC LEADERSHIPIGJC 2019 is being organized under the leadership of the Executive Committee led by Leigh Anne Swayne, University of Victoria, and Christian Naus, University of British Columbia with the support of the Scientific Program Committee.

IGJC Executive Committee

Co-Chair Leigh Anne Swayne | University of Victoria

Co-Chair Christian Naus | University of British Columbia

Dale Laird | University of Western Ontario

Paul Lampe | Fred Hutchinson Cancer Research Center, University of Washington

Scientific Program Committee

Trond Aasen | Vall d’Hebron Research Institute

Viviana Berthoud Barrandeguy | University of Chicago

Maria Daglia | University of Sao Paulo

Brant Isakson | University of Virginia School of Medicine

Michael Jackson | Max Rady College of Medicine, University of Manitoba

Michael Koval | Emory University School of Medicine

Patricia Martin | Glasgow Caledonian University

Maria Mayan | Institute of Biomedical Research of A Coruna (INIBIC)

Silvia Penuela | University of Western Ontario

James Smyth | Virginia Tech Carilion Research Institute and School of Medicine

Arantxa Tabernero | Institute of Neuroscience INCYL, University of Salamanca

Roger Thompson | University of Calgary

IGJC 2019 Secretariat

Podium Conference Specialists

• Michelle Smith

• Marischal De Armond

• Alaina Laflamme

GENERAL CONFERENCE INFORMATION

CONFERENCE CENTER FLOOR PLAN CONFERENCE VENUEVictoria Conference Centre

720 Douglas Street Victoria, BC V8W 3M7

All conference sessions will take place in this location.

LeveL One | 23,500 ft2

2,183 m2

not to scale

n

Meeting planner’s office

audio/visual services

1

2

3 videoconference suite

the shops the shopsthe shops

oak bay 1 2saanich

1

2

registra

tion

toteM

area

pre-function 1b

to thefairMonteMpress

douglas street entrance

to crystal garden

lecturetheatre

esquiMalt

victoria

soo

ke

2 3

upperpavilion

lowerpavilion

outdoor courtyard

1pre-function

1a

client/ event

services

DimensiOns | AreA cApAcity by set-up

ft / in ft2 m2 t/s c/R BRD H/s U BanQ Rec 10 x 10 8 x 10 8 x 8 tt PB

lecture theatre 3,700 ft2 344 m2 400tHeatRe stage 24' x 24' 576 ft2 54 m2

saanich 61' x 35' x 9'5" 2,135 ft2 199 m2 227 132 70 60 150 225 10 10 12 25 24saanicH 1 43' x 35' x 9'5" 1,505 ft2 140 m2 163 96 54 42 120 150 7 7 9 19 15saanicH 2 35' x 18' x 9'5" 630 ft2 59 m2 54 36 28 30 65 3 3 4 6 6

sooke 18' x 13' x 9'5" 234 ft2 22 m2 10 10oak bay 50' x 37' x 9'5" 1,850 ft2 172 m2 169 96 56 42 120 200 8 12 14 20 20

oak Bay 1 37' x 25' x 9'5" 925 ft2 86 m2 80 54 36 30 60 90 3 6 7 10 10oak Bay 2 37' x 25' x 9'5" 925 ft2 86 m2 80 54 36 30 60 90 3 6 7 10 10

esquiMalt 38' x 28' x 9'5" 1,064 ft2 99 m2 96 60 42 34 60 110 6 6 6 12 10victoria 24' x 16' x 9'5" 384 ft2 36 m2 35 18 20 20 40 1 1 2 5 2

west coast ceiling ht. 9’ 980 ft2 91 m2 50 36 20 18 21 30 8 8pre-function 1 ceiling ht. 9'10" 6,500 ft2 604 m2 240 400 16 20 26 30 34PRe-fUnction 1a ceiling ht. 9'10" 4,875 ft2 453 m2 200 300 12 16 22 24 28PRe-fUnction 1B ceiling ht. 9'10" 1,625 ft2 151 m2 40 100 4 4 4 6 6

registration 370 ft2 34 m2

planner’s office 13' x 6' x 7' 78 ft2 7 m2

toteM area 110' x 23' 2,530 ft2 235 m2 250videoconference 17' x 11' x 8' 187 ft2 17 m2 10

upper pavilion 59' x 65' 3,875 ft2 360 m2 290 375 20 25 28 55 37lower pavilion 69' x 65' 3,741 ft2 348 m2 210 375 13 16 22 40 36

courtyard* 7,730 ft2 718 m2 340 700

occupancy numbers allow for basic a/V and staging. *open air courtyard on two levels.

victoria conference centre

720 douglas street victoria bc canada v8w 3M7 1.866.572.1151 or 250.361.1000 fax 250.361.1099

www.victoriaconference.com

the shopswestcoast

LeveL tWO | 24,500 ft2

2,276 m2

DimensiOns | AreA cApAcity by set-up

ft / in ft2 m2 t/s c/R BRD H/s U BanQ Rec 10 x 10 8 x 10 8 x 8 tt PB

carson hall 145' x 96' x 23' 14,689 ft2* 1,365 m2* 1,500 816 138 111 1,010 2,000 80 102 122 190 166salon a 96' x 48' x 23' 5,056 ft2* 470 m2* 500 264 82 68 370 600 29 30 38 56 55salon B 96' x 49' x 23' 5,025 ft2* 466 m2* 494 270 82 68 340 600 25 34 42 64 53salon c 96' x 48' x 23' 4,608 ft2 428 m2 468 294 82 68 320 600 23 32 39 63 50

salon aB 97' x 96' x 23' 10,081 ft2* 936 m2* 1,053 588 112 81 740 1,200 61 68 86 134 118salon Bc 97' x 96' x 23' 9,633 ft2* 895 m2* 1,008 540 112 81 730 1,200 56 62 81 130 113

sidney 35' x 27' x 10' 945 ft2 88 m2 85 60 42 34 60 110 4 4 6 11 10langford 24' x 15' x 9' 360 ft2 34 m2 30 18 20 20 30 1 2 2 4 4

Metchosin 24' x 15' x 9' 360 ft2 34 m2 30 18 20 20 30 1 2 2 4 4colwood 35' x 27' x 10'6" 945 ft2 88 m2 90 60 28 42 34 60 110 4 4 6 10 10colwooD 1 27' x 17'5" x 10'6" 472 ft2 44 m2 48 24 20 22 30 50 2 2 3 7 4colwooD 2 27' x 17'5" x 10'6" 472 ft2 44 m2 48 24 20 22 30 50 2 2 3 7 4

view royal 28' x 24' x 9' 672 ft2 63 m2 60 36 20 32 22 40 60 3 3 5 7 8pre-function 2 6,880 ft2 640 m2 170 800 15 18 19 44 39PRe-fUnction 2a 2,900 ft2 269 m2 90 400 8 10 10 19 20PRe-fUnction 2B 2,800 ft2 260 m2 70 400 5 6 7 21 14PRe-fUnction 2c 1,180 ft2 109 m2 10 2 2 2 4 5

occupancy numbers allow for basic a/V and staging. *including alcoves.

not to scale

n

vcc kitchen

carson hall

salonb

salona

pre-function 2c

sidneyMetchosin

loading dock

colwood 1 2

view royallangford

salonc

pre- function

2a

loading raMp

pre-function 2b

balcony

LEVEL 1

LEVEL 2




REGISTRATIONConference registration fees include access to all sessions including keynote, plenary, and poster sessions. Registration also includes the welcome reception, coffee breaks, lunches, and the conference closing dinner.

ADDITIONAL TICKETS Tickets can be purchased separately for your guests and/or children for the welcome reception, closing dinner and excursions. These additional tickets can be purchased from the staff at IGJC’s Registration Desk.

NAME BADGES Your name badge is your admission ticket to the conference sessions, coffee breaks, meals, and receptions. Please wear it at all times. At the end of the Conference we ask that you recycle your name badge in one of the name badge recycling stations that will be set out or leave it at the Registration Desk.

To help identify and mentor our future investigators, trainee (students and post docs) delegates have blue edged badges. All other delegates have clear badges. IGJC Executive, Sponsors and Staff will be identified by appropriate ribbons.

DRESS CODE Dress is casual for all IGJC meetings and social events.

REGISTRATION AND INFORMATION DESK HOURS The IGJC Registration and Information Desk, located in the pre-function space, outside the poster room of the Victoria Conference Centre, will be open during the following dates and times:

Saturday, July 27 14:30 – 18:00Sunday, July 28 08:00 – 18:00Monday, July 29 08:00 – 18:00Tuesday, July 30 08:00 – 13:00Wednesday, July 31 08:00 – 17:00

If you need assistance during the conference, please visit the Registration Desk.

POSTER INFORMATION Posters will be up for the duration of the meeting with two poster presentation times on Sunday July 28 and Monday July 29. Poster presenters must set-up and remove their posters during the following times.

Set-up: Sunday, July 28, 08:00 and 08:45Remove: Wednesday, July 31, by 15:00

Any posters that are not taken down by the removal deadline will be held at the registration desk until the end of the conference. Any posters that remain unclaimed by the end of the conference will be disposed of.

Information on Poster Authors (Lead), Poster Numbers and Poster Titles begins on page 30. For a complete copy of all the poster abstracts, a digital abstract booklet can be downloaded from the IGJC website at igjconference.org

Easy reference Poster floor plans can be found on the inside back cover of this program.

STAFF IGJC staff from Podium Conference Specialists can be identified by orange ribbons on their name badges. Feel free to ask anyone of our staff for assistance. For immediate assistance please visit us at the Registration Desk.

NEARBY AMENITIES:Liberty Cafe Immediately outside of the Victoria Conference Centre

Restaurants Multiple restaurants can be found on Douglas Street or Government Street (behind the Conference Centre) in a variety of price points.

Thrifty Foods Supermarket 475 Simcoe Street (15 minute walk)

Market on Yates Supermarket 905 Yates Street (15 minute walk)

INTERNET SERVICES Wireless Internet is available to IGJC delegates for no charge. Simply choose the VictoriaConference WiFi network, Password – IGJC2019. Kindly note, the WiFi strength is ideal for checking emails and websites but is not strong enough for streaming videos or heavy social media use.

If you are active on social media, make sure to hashtag #IGJC2019 @int_gap when referring to the meeting. We ask that all IGJC delegates check with the author prior to tweeting about any presentations.

If you require assistance, please visit the registration desk and we will endeavour to assist you.

NO SMOKING POLICY The Victoria Conference Centre is a completely non-smoking facility.

GENERAL CONFERENCE INFORMATION

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SOCIAL EVENTS

Saturday, July 27

17:00 – 18:00 WELCOME RECEPTIONLocation: 2nd Floor of the Victoria Conference Centre. Stop by the registration desk to pick up your free drink ticket

18:30 – 20:30 GRAZING STYLE OPENING DINNERLocation: Victoria Conference Centre, Pavilion, 1st Floor

Wednesday, July 31

19:00 – 22:00 CLOSING CONFERENCE DINNERLocation: Fairmont Empress, Crystal Ballroom & Palm Court

CONFERENCE EXCURSIONSIGJC is pleased to announce that Tuesday July 30 is only a half day of sessions. Use the free time to explore the beauty of Victoria through one of the arranged excursions or explore the city on your own time. Excursions can be purchased at the conference registration desk.

Butchart Gardens - $72/Ticket

13:00 – 16:30

The Butchart Gardens Tour will depart from the Victoria Conference Centre at 13:00. Please meet by the Totem Pole at 12:45 for departure at 13:00.

World Famous Butchart Gardens is a wonderland containing spectacular theme gardens, exotic plants, streams and ponds. Ross Fountain, the famous Sunken Garden, Rose Garden, Japanese Garden and much more are sure to create a lasting impression. Your ticket cost includes admission to the gardens as well as tour bus transportation to and from the gardens.

City Tour Bus - $47/Ticket

13:00 – 16:30

The City Tour will depart from the Victoria Conference Centre at 13:00. Please meet by the Totem Pole at 12:45 for departure at 13:00.

Experience Victoria through our fully guided city tour aboard a mini-coach bus. Drive through downtown Victoria, Chinatown and Old Town, as well as Fisherman’s Wharf while hearing stories of famous Victorians. Travel beyond the city core and out to view Christ Church Cathedral and Oak Bay Village, our little part of Britain. Guests will depart and be returned to the Victoria Conference Centre.

Whale Watching - $100/Ticket

13:30 – 17:00

The Whale Watching Tour will depart from the Victoria Conference Centre at 13:45. Please meet by the Totem Pole at 13:30 for departure to the boats at 13:45.

Join us aboard a comfortable double hulled catamaran for a fast, smooth and stable ride on the Salish Sea, one of the world’s largest and biologically rich inland seas. The wildlife that can be found here includes Resident (fish-eating) Orcas, Transient (Marine mammal eating) Orcas, Humpback Whales, Gray Whales, Minke Whales, Harbor & Dall’s Porpoise, Pacific White-sided Dolphins, Stellar & California Sea Lions, Harbor Seals, Northern Elephant Seals and both River & Sea Otters. The tour will begin by meeting in front of the totem poles at the Victoria Conference Centre before walking over to the boat for departure.

Lunch Restaurants

To encourage delegates to explore Victoria, get some fresh air and provide an opportunity to choose the food that works best for multiple food intolerances and allergies, IGJC 2019 has partnered with four local restaurants to offer lunch coupons. Each restaurant is a short walk from the Victoria Conference Centre and they can all provide a quick meal so you can enjoy the extended lunch break!

Each coupon is good for $20 (inclusive of tax) worth of food and drinks at one of the four restaurants listed below. Simply inform your server that you will be using the coupon, order your food and enjoy! If your bill is over $20 you are responsible for any additional charges but if it is not, we hope you enjoyed your meal!

BROWNS SOCIAL HOUSE809 Douglas Street

With an outdoor patio and a variety of Asian fusion and perfect lunch cuisine, Browns is a great place to refuel for the afternoon sessions.

CORA BREAKFAST AND LUNCH VICTORIA850 Douglas Street

Big portions and healthy food! This is the best location if you missed breakfast or would like a healthy meal that won’t leave you wanting for anything. Lunch meals are served with a choice of soup or dessert.

OLD SPAGHETTI FACTORY703 Douglas Street

With an all included menu, you get the most food for your coupon! All entrees include bread, soup or salad, ice cream and coffee or tea. Menu items include entrée salads, pastas, grilled items and sandwiches.

STICKY WICKET RESTAURANT, PART OF THE STRATHCONA HOTEL919 Douglas Street

The Sticky Wicket is a cricket themed pub and restaurant offering seasonal west coast food, cocktails and an array of international, craft, micro and domestic beers. With a multi page menu, there is something for everyone available!




For subscriptions and consortia sales email [email protected]

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ANNUAL CONFERENCE SCHEDULE

Saturday July 27, 2019

16:30 – 17:00 Salon C

OPENING CEREMONY

17:00 – 18:00 Prefunction 2b

(2nd floor)

OPENING DRINK RECEPTION

18:30 – 20:30 Lower Pavilion

GRAZING DINNER

Sunday July 28, 2019

08:45 – 09:30 Salon C

KEYNOTE LECTURE 1Chair: Christian Naus | University of British Columbia

Brenda Kwak | University of Geneva

Canonical and Non-Canonical functions of CX37 in atherosclerosis

09:30 – 09:45 STAR AWARD TALKChair: Christian Naus | University of British Columbia

Erin Mulkearns-Hubert | Cleveland Clinic Foundation

Development of CX46 targeting strategy for cancer stem cells

09:45 – 10:00 COFFEE BREAK

10:00 – 11:30 SESSION 1Chair: James Smyth | Virginia Polytechnic Institute and State University

S1.1 Intracellular endothelial Pannexin 3 can regulate vasomotor function in resistance arteries

Presenter: Abigail Wolpe

Abigail Wolpe¹, Matteo Ottolini¹, Swapnil Sonkusare², Scott Johnstone², Kevin Barr³, Frank Beier3, Silvia Penuela³, Brant Isakson²¹University of Virginia, ²University of Virginia School of Medicine, ³University of Western Ontario




10:00 – 11:30 SESSION 1 cont’d

S1.2 Cx43-dependent disruption of intracellular calcium homeostasis in the right ventricle as a mechanism of arrhythmogenic right ventricular cardiomyopathy (ARVC) in plakophilin-2-deficient mice.

Presenter: Mario Delmar

Joonchul Kim¹, Marta Perez-Hernandez², Mingliang Zhang¹, Francisco Javier Alvarado2, Hector Valdivia¹, Marina Cerrone¹, Mario Delmar²¹New York University School of Medicine, ²University of Wisconsin/Madison

S1.3 The Cx43 Carboxyl-terminus protects endothelial barrier function via a ZO-1 interaction-dependent modulation of Cx43 Hemichannel activity

Presenter: Randy Strauss

Randy Strauss¹, Jane Jourdan1, Robert Gourdie¹¹Fralin Biomedical Research Institute, Virginia Tech

S1.4 S-nitrosylation of Connexin43 hemichannels elicits cardiac stress-induced arrhythmias in Duchenne Muscular Dystrophy mice

Presenter: Mauricio Lillo

Mauricio Lillo¹, Eric Himelman¹, Natalia Shirokova¹, Lai-Hua Xie¹, Diego Fraidenraich¹, Jorge Contreras¹¹Rutgers University

S1.5 E2F mediated regulation of connexin 43 and dilated cardiomyopathy

Presenter: Balwant Tuana

Balwant Tuana¹¹University of Ottawa

S1.6 Connexin43 phosphorylation at S330 regulates whole cellular ERK and Akt activity and cell survival in response to ischemia

Presenter: Paul Lampe

Joell Solan¹, Lucrecia Márquez-Rosado¹, Paul Lampe¹¹Fred Hutchinson Cancer Research Center

11:30 – 13:15 LUNCH ON OWN

13:15 – 14:45 SESSION 2Chair: Maria Mayan Santos | Instituto Investigacíon Biomédica A Coruña

S2.1 PKA phosphorylation of Cx43 and its interaction with VASP reduces cell migration but increases directionality

Presenter: Kristin Pogoda

Kristin Pogoda¹, Ulrich Pohl¹, Petra Kameritsch¹¹Ludwig-Maximilians-University Munich

S2.2 Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions

Presenter: Li Zheng

Li Zheng¹, Hanjun Li², Andrew Cannon¹, Andrew Trease¹, Gaelle Spagnol¹, Hong Zheng³, Stanley Radio¹, Kaushik Patel¹, Surinder Batra¹, Paul Sorgen¹¹University of Nebraska Medical Center, ²Duke University, ³University of South Dakota

S2.3 Endothelial pannexin 1 channels controls inflammation by regulating intracellular calcium

Presenter: Yang Yang

Yang Yang¹, Angela Best-Campbell¹, Edgar Macal Jr¹, Leon DeLalio², Daniela Begandt¹, Jenna Milstein¹, Iona Donnelly³, Ashley Miller³, Martin McBride³, Xiaohong Shu¹, Michael Koval¹, Brant Isakson², Scott Johnstone²¹University of Virginia, ²University of Virginia School of Medicine, ³University of Glasgow

S2.4 Platelet connexins are regulated through a network of proteases, kinases and phosphatases

Presenter: Amro Elgheznawy

Amro Elgheznawy¹, Jonathan Gibbins¹¹University of Reading

S2.5 Dynamic UTR usage regulates alternative translation to modulate gap junction formation during stress and aging

Presenter: Michael Zeitz

Michael Zeitz¹, Patrick Calhoun¹, Carissa James¹, Thomas Taetzsch¹, Kijana George¹, Stefanie Robel¹, Gregorio Valdez¹, James Smyth¹¹Fralin Biomedical Research Institute at Virginia Tech






S2.6 Cardiac ischemia modulates the sorting of Cx43 into EVs

Presenter: Henrique Girao

Steve Catarino¹, Teresa Ribeiro-Rodrigues¹, Lino Gonçalves1, Tania Martins-Marques¹, Henrique Girão¹¹Faculty of Medicine, University of Coimbra

14:45 – 15:00 COFFEE BREAK15:00 – 16:30 SESSION 3

Chair: Hung-I Yeh | Mackay Memorial Hospital

S3.1 Connexin mimetic peptide disruption of human smooth muscle cell proliferation and neointimal formation

Presenter: Scott JohnstoneYang Yang¹, George Baillie², Paul Lampe³, James Smyth⁴, Michael Koval⁵, Brant Isakson⁶, Scott Johnstone⁶¹University of Virginia, ²University of Glasgow, ³Fred Hutchinson Cancer Research Center, ⁴Fralin Biomedical Research Institute at Virginia Tech, ⁵Emory University School of Medicine, ⁶University of Virginia School of Medicine

S3.2 Pannexin 1 activates a purinergic venous-specific cascade to induce endothelial permeability

Presenter: Michael KovalMichael Koval¹, Daniela Begandt², H Skye Comstra¹, Samuel Molina¹, Nenja Kruger³, Lauren Biwer³, Scott Johnstone³, Alexander Lohman⁴, Miranda Good³, Leon DeLalio³, Kwangseok Hong³, Hannah Bacon³, Swapnil Sonkusare³, Brant Isakson³¹Emory University School of Medicine, ²University Hospital, LMU Munich, ³University of Virginia School of Medicine, ⁴University of Calgary

S3.3 Pannexin channel inhibition to prevent triggered arrhythmia

Presenter: Grace SalzerGrace Salzer¹, Jeevan Jadapalli¹, Bjorn Knollmann¹, Ganesh Halade¹, Sabine Huke¹, Lufang Zhou¹¹University of Alabama Birmingham

15:00 – 16:30 S3.4 Connexin37-dependent mechanisms contribute to modulate Angiotensin II-Mediated Hypertension

Presenter: Jacques-Antoine HaefligerLoïc Le Gal¹, Maxime Pellegrin¹, Tania Santoro¹, Lucia Mazzolai¹, Armin Kurtz², Charlotte Wagner¹, Paolo Meda³, Jacques-Antoine Haefliger¹¹CHUV, ²University of Regensburg, ³University of Geneva

S3.5 Development of ADMix cell-based gap junctional intercellular communication assay conducive to high-throughput screening

Presenter: Yunkyung HeoYunkyung Heo¹, Eun Ju Choi¹, Jinu Lee¹¹Yonsei University

S3.6 The amino terminal domain plays an important role in Cx45 gap junction channel gating kinetics

Presenter: Donglin BaiArtur Santos-Miranda¹, Honghong Chen¹, Donglin Bai¹¹University of Western Ontario

16:30 – 18:00 Salon A & B

POSTER SESSION

Monday July 29, 2019

08:45 – 09:30 Salon C

KEYNOTE LECTURE 2Chair: Leigh Anne Swayne | University of Victoria

Douglas Bayliss | Joseph & Francis Larner Professor & Chair of Pharmocology, University of Virginia

Pannexin channels: Properties & mechanisms of activation

09:30 – 09:45 STAR AWARD TALKChair: Leigh Anne Swayne | University of Victoria

Juan Sanchez-Arias | University of Victoria

Pannexin 1 regulates neuronal networks and dendritic spine formation in cortical networks






10:00 – 11:30 SESSION 4Chair: Lisa Ebihara | Rosalind Franklin University of Medicine and Science

S4.1 Connexin-46/50 intercellular communication channels in a near-native lipid environment resolved by CryoEM

Presenter: Steve Reichow

Jonathan Flores¹, Kimberly Dolan, Bassam Haddad2, Janette Myers2, Craig Yoshioka3, Daniel Zuckerman3, Steve Reichow¹¹Portland State University / Oregon Health and Science University, ²Portland State University, 3Oregon Health and Science University

S4.2 Studying the electrostatic potential effects of calcium binding on hCx26 hemichannels

Presenter: Jorge Carrasco

Jorge Carrasco¹, Felipe Villanelo², Tomas Pérez-Acle²¹Fundacion Ciencia & Vida, ²Fundacion Ciencia & Vida - Centro Interdisciplinario de Neurociencia de Valparaíso

S4.3 The characterization of Panx1 and Panx3 in skin using two knockout mouse models

Presenter: Brooke O'Donnell

Brooke O’Donnell¹, Charles Wakefield¹, Julia Abitbol¹, John Kelly¹, Rafael Sanchez-Pupo¹, Danielle Johnston¹, Kevin Barr¹, Silvia Penuela¹¹University of Western Ontario

S4.4 Pannexin 1 is differentially regulated by unique splice variants of Src kinase

Presenter: Andrew KJ Boyce

Andrew KJ Boyce¹, Roger Thomspon¹¹University of Calgary

S4.5 Characterization and functional study of the Gap Junction Gene Innexin in Lymnaea stagnalis

Presenter: Fenglian Xu

Brittany Mersman¹, Sonia Jolly¹, Zhenguo Lin¹, Fenglian Xu¹¹Saint Louis University

10:00 – 11:30 S4.6 Connexin and Pannexin modulation of extracellular potassium concentrations and redistribution in the cerebral cortex in vivo

Presenter: Peter Carlen

Azin Amini¹, Paolo Bazzigaluppi², Bojana Stefanovic², Philippe Monnier¹, Georg Zoidl³, Peter Carlen¹¹Krembil Research Institute, ²Sunnybrook Research Institute, ³York University

11:30 – 11:45 NSF GRANT OPPORTUNITIES FOR US-BASED INVESTIGATORSMatthias Falk | NSF Program Officer

11:45 – 13:15 LUNCH ON OWN

13:15 – 14:45 SESSION 5Chair: Jessica Esseltine | Memorial University of Newfoundland

S5.1 Functional plasticity of Cx36 gap junctions is regulated by the actin cytoskeleton

Presenter: John O'BrienJaya Aseervatham¹, Helen Wang¹, Cheryl Mitchell¹, Ya-Ping Lin¹, John O’Brien¹¹University of Texas Health Science Center at Houston

S5.2 Cryo-EM structures of undocked innexin-6 gap junction hemichannels

Presenter: Atsunori OshimaAtsunori Oshima¹, Batuujin Burendei¹, Ruriko Shinozaki¹, Masakatsu Watanabe², Tohru Terada³, Kazutoshi Tani¹, Yoshinori Fujiyoshi¹¹Nagoya University, ²Osaka University, ³Tokyo University

S5.3 Intra-domain interactions of the Cx37 Carboxyl-Terminus necessary for growth suppressive function alter channel gating

Presenter: Tasha PontiflexTasha Pontifex¹, Nicole Jacobsen², Samantha Taylor¹, Janis Burt¹¹University of Arizona, ²University of Missouri

S5.4 Connexin mutants lead to compromise of the lens circulation, calcium accumulation/precipitation, and cataracts

Presenter: Viviana BerthoudViviana Berthoud¹, Junyuan Gao², Peter Minogue¹, Oscar Jara¹, Richard Mathias², Eric Beyer¹¹University of Chicago, ²Stony Brook University






S5.5 Disease modelling of deafness with iPS cell derived gap junction forming cells

Presenter: Kazusaku KamiyaKazusaku Kamiya¹¹Juntendo University Faculty of Medicine

S5.6 Cx30 is required for neural and synapse development in the inner ear

Presenter: Hong-Bo ZhaoHong-Bo Zhao¹¹University of Kentucky Medical Center


SESSION 6Chair: Jorge Contreras | Rutgers University

S6.1 Neural gap junction genes regulate ageing in C. elegans

Presenter: Karl Emanuel Busch

Nathalie Vladis¹, Katharina Elisabeth Fischer¹, Karl Emanuel Busch¹¹University of Edinburgh

S6.2 Investigating Cx43 HC and GJ localization and function in Amyotrophic Lateral Sclerosis

Presenter: Jessica Joseph

Jessica Joseph1, Mauricio Lillo Gallardo2, Akshata Almad3, Arens Taga1, Aayush Pokharel1, Sarah Gross1, Jorge Contreras2, Nicholas J Maragakis11Johns Hopkins University, 2Rutgers University, 3Children’s Hospital of Philadelphia

S6.3 Targeting connexins at the endothelial-astrocyte interface protects against inflammatory blood-brain barrier leakage

Presenter: Luc Leybaert

Marijke De Bock¹, Maarten De Smet², Valerie Van Haver², Stijn Verwaerde², Roosmarijn Vandenbroucke², Luc Leybaert²¹Johnson & Johnson, ²Ghent University


15:00 – 16:30 S6.4 Altered connexin43 function in post-traumatic brain injury induced epilepsy

Presenter: Carmen Munoz-Ballester

Carmen Muñoz-Ballester¹, Oleksii Shandra¹, Robert Gourdie1, James Smyth1, Samy Lamouille¹, Stefanie Robel1¹Fralin Biomedical Research Institute at Virginia Tech

S6.5 A bidirectional plasticity rule for central mammalian electrical synapses

Presenter: Julie Haas

Brandon Fricker¹, Emily Heckman¹, Julie Haas¹¹Lehigh University

S6.6 Connexin hemichannels and pannexin channels as drug targets in liver toxicity and disease

Presenter: Mathieu Vinken

Mathieu Vinken¹, Bruno Cogliati²¹Vrije Universiteit Brussel, ²University of São Paulo

16:30 – 18:00 Salon A & B

POSTER SESSION

Tuesday July 30, 2019

08:45 – 09:30 Salon C

KEYNOTE LECTURE 3Chair: Dale Laird | University of Western Ontario

Jean X Jiang | Ashbel Smith Professor, UT Health Science Center at San Antonio (UTHSCSA), Associate Director, Joint Biomedical Engineering Graduate Program of UTSA/UTHSCSA

Connexin hemichannels in cellular hemostasis, mechanotransduction and cancer metastasis

09:30 – 09:45 STAR AWARD TALKChair: Dale Laird | University of Western Ontario

Cherie Brown | York University

Sensorimotor behavior in the Connexin-35B (CX35B) Knock-out Zebrafish (Danio Rerio)





10:00 – 11:30 SESSION 7Chair: Maria Dagli | University of Sao Paulo

S7.1 Regulation of malignant features by Cx43 in cancer: CRISPR-Cas9 knockout cell lines identifies Cx43 as a regulator of pro-tumorigenic tunnelling nanotubes

Presenter: Trond Aasen

Daniel Domínguez Azorín¹, Laia Vidal-Brime¹, María José Muñoz Guijarro¹, Alexander Tishchenko¹, Chris Pearce¹, Trond Aasen¹¹Vall d’Hebron Research Institute (VHIR)

S7.2 A 3D culture model to investigate Cx43 in glioma invasion

Presenter: Wun Chey Sin

Kanika Khosla¹, Derek Van Pel¹, Christian Naus¹, Wun Chey Sin¹¹University of British Columbia

S7.3 Activation of osteocytes Connexin43 Hemichannels suppresses breast cancer bone metastasis, a potential therapeutic target

Presenter: Manuel Riquelme

Manuel Riquelme¹, Sumin Gu1, Jeffrey Chavez¹, Zhiqiang An2, Jean Jiang¹¹UT Health Science Center San Antonio, 2Brown Foundation, UT Health Houston

S7.4 Role of tunneling nanotubes containing gap junctions in glioblastoma resistance to therapy

Presenter: Silvana Valdebenito

Silvana Valdebenito¹, Krishna Bhat², Eliseo Eugenin¹¹University of Texas Medical Branch, ²The University of Texas MD Anderson Cancer Center

S7.5 Connexin43 peptide, TAT-Cx43-266-283, selectively targets glioma cells and impairs malignant growth in mouse models of glioma in vivo

Presenter: Arantxa Tabernero

Myriam Jaraíz-Rodríguez¹, Rocío Talaverón¹, Laura García-Vicente¹, Sara Pelaz¹, Marta Domínguez-Prieto¹, Wun Chey Sin², John Bechberger², José Medina¹, Christian Naus², Arantxa Tabernero¹¹University of Salamanca, ²University of British Columbia


10:00 – 11:30 S7.6 Connexin43 regulates inflammasome activation and inflammatory cell injury through modulation of NADPH Oxidase-dependent Redox Signaling Pathway

Presenter: Yanru HuangYanru Huang¹, Zhimin Mao¹, Xiawen Yang², Jian Yao¹¹Interdisciplinary Graduate School of Medicine, University of Yamanashi, ²University of Yamanashi

11:30 – 13:15 LUNCH ON OWN

13:00 – onwards FREE TIME OR ORGANIZED EXCURSIONS

Wednesday July 31, 2019

08:45 – 09:30 Salon C

KEYNOTE LECTURE 4Chair: Paul Lampe | Fred Hutchinson Cancer Research Center, University of Washington

Robert Gourdie | Director of the Center for Heart and Reparative Medicine Research (CHARMR) at the Fralin Biomedical Research Institute at VTC(FBRI) and Professor in the Department of Biomedical Engineering and Mechanics at Virginia Tech

Connexin43: For better or worse, in sickness and in health…

09:30 – 09:45 STAR AWARD TALKChair: Paul Lampe | Fred Hutchinson Cancer Research Center, University of Washington

Marta Varela-Eirin | INIBIC

Connexin43 – Positive exosomes efficiently induce cellular senescence via P53/P16 (INK4A) in target cells contributing to osteoarthritis progression


10:00 – 11:30 SESSION 8Chair: Justin Lathia | Cleveland Clinic Foundation

S8.1 The human homologue of Drosophila Disc Large (hDlg) and human papillomavirus E6 proteins control intercellular communication via trafficking and degradation of connnexin 43

Presenter: Li DongLi Dong¹, Andrew Stevenson¹, Paola Massimi², Lawrence Banks², Scott Johnstone³, Sheila Graham¹¹MRC-University of Glasgow, ²International Centre for Genetic Engineering and Biotechnology, ³University of Virginia School of Medicine





S8.2 Targeting the Cx26/NANOG/Focal Adhesion Kinase complex in triple negative breast cancer to attenuate self-renewal via cell penetrating peptides

Presenter: Emily Esakov

Emily Esakov¹, Praveena Thiagarajan¹, Erin Mulkearns-Hubert¹, Justin Lathia¹, Ofer Reizes¹¹Cleveland Clinic Foundation

S8.3 Replacement of endogenous with C-terminus Truncated Connexin 43 in Zebrafish: Expected, and unexpected results

Presenter: Matthias Falk

Matthias Falk¹, Kathryn Iovine¹¹Lehigh University

S8.4 Mechanism by which Connexin43 function in the medial blastema influences joint positioning

Presenter: Shashwati Bhattacharya

Shashwati Bhattacharya¹, Kathryn Iovine¹¹Lehigh University

S8.5 Pannexin1 is a novel regulator of the Wnt signaling pathway in melanoma

Presenter: Samar Sayedyahossein

Samar Sayedyahossein¹, Danielle Johnston1, Kenneth Huang¹, Zameena Lakhani¹, Daniel Nouri-Nejad1, Rafael Sanchez Pupo¹, Silvia Penuela1¹University of Western Ontario

S8.6 IL-10 and TGFβ differentially regulate gap junction formation and membrane transfer in macrophages and macrophage-like cells

Presenter: Steven Taffet

Steven Taffet¹, Elizabeth Eill¹¹SUNY Upstate Medical University

11:30 – 13:15 LUNCH ON OWN


13:15 – 14:45 SESSION 9Chair: Brant Isakson | University of Virginia

S9.1 Connexin and Pannexin signalling in Keratinocytes and Eccrine cells following pro-inflammatory challenge

Presenter: Carmela Errico

Carmela Errico¹, Laura Garcia-Vega¹, Patricia Martin¹¹Glasgow Caledonian University

S9.2 Induction of Cx26 hemichannels in respiratory airway epithelial cells by stimulation of the A2B adenosine receptors

Presenter: Anaclet Ngezahayo

Anne Dirks¹, Tina Lehrich¹, Almke Bader², Anaclet Ngezahayo¹¹Leibniz University Hannover, ²Hannover Medical School

S9.3 Pannexin1 deletion from TRAP-expressing cells results in increased osteoclast differentiation and function, and reduced bone mass only in female mice

Presenter: Lilian Plotkin

Lilian Plotkin¹, Padmini Deosthale¹, Jung Min Hong², Harry Sidhu¹, Alyson Essex¹, Angela Bruzzaniti²¹Indiana University School of Medicine, ²Indiana University School of Dentistry

S9.4 Opposite effect of connexin43 in the differentiation of stem cells into hepatocyte-like cells and trabecular meshwork-like cells

Presenter: Xinbo Li

Xinbo Li¹, Mengzhou Xue², Mary Kelley¹, Ted Acott¹, James Nagy³, Fengyan Zhang⁴¹Casey Eye Institute, Oregon Health & Science University, ²Dept. of Cerebrovascular Diseases, the Second Affiliated Hospital, Zhengzhou University, ³Casey Eye Dept. of Physiology and Pathophysiology, University of Manitoba, ⁴Department of Ophthalmology, First Affiliated Hospital of Zhengzhou University

S9.5 Multiple mechanisms involving three connexin family members are responsible for erythrokeratodermia variabilis et progressiva

Presenter: Qing Cindy Shao

Qing Cindy Shao¹, Akina Au¹, Biao An¹, Bindra Shah¹, Kyra White¹, Nicole Kuntz¹, Ruth Neumann¹, Samina Nazarali¹, Dale W. Laird¹¹The University of Western Ontario





S9.6 Connexin43 controls body adiposity, glucose tolerance and bone modeling via channel and scaffolding functions

Presenter: Roberto Civitelli

Seung-yon Lee¹, Manuela Fortunato¹, Marcus Watkins¹, Francesca Fontana¹, Roberto Civitelli¹¹Washington University in St. Louis


15:00 – 16:15 SESSION 10Chair: Michael Koval | Emory University School of Medicine

S10.1 Targetting Cx43 hemichannels with Gap19 and blocking MAPK phosphorylation reveal a common mechanism for neuroprotection in stroke.

Presenter: Christian Naus

Moises Freitas-Andrade¹, Nan Wang², John Bechberger³, Marijke DeBock², Paul Lampe⁴, Luc Leybaert², Christian Naus³¹University of Ottawa, ²Ghent University, ³University of British Columbia, ⁴Fred Hutchinson Cancer Research Center

S10.2 Is the inflammatory response the cause of tissue dysfunction in several chronic diseases?

Presenter: Juan Sáez

Juan Sáez¹¹Universidad de Valparaíso

S10.3 Cx43 Secretome as a mediator therapeutic resistance & malignancy in glioma

Presenter: Vincent Chen

Vincent Chen¹¹Brandon University

S10.4 Connexin43 mimetic peptide alters fundamental scar tissue structure through modulation of dermal fibroblast behavior

Presenter: Jade Montgomery

Jade Montgomery¹, Katherine Degen¹, Linda Jourdan², Robert Gourdie²¹Virginia Tech, ²Fralin Biomedical Research Institute


15:00 – 16:15 S10.5 Novel inhibitors of Cx43-Ezrin-PKA complex for treatment of patients with glioblastoma

Presenter: Aleksandra Dukic

Aleksandra Dukic¹, Rafi Ahmad², Kjetil Tasken1¹University of Oslo, Institute of Cancer Research, ²Inland Norway University of Applied Sciences

16:15 – 17:00 2021 FUTURE CONFERENCE PRESENTATION

17:00 – 17:30 CLOSING REMARKS

19:00 – 22:00 Crystal Ballroom of the Fairmont

Empress

CONFERENCE DINNER




KEYNOTE PRESENTATIONS

Brenda Kwak | University of Geneva

Brenda Kwak is Professor at the University of Geneva Medical School in Geneva, Switzerland. Her research has focused for many years on the function of connexins and pannexins in cardiovascular physiology and pathology. In recent years, her research lab investigated the regulation of these proteins

by shear stress and their role in the pathogenesis of atherosclerosis, lymphedema, thrombosis and cardiac reperfusion injury.

Cannonical and non-cannonical functions of CX37 in atherosclerosis

Epidemiological studies have indicated that a single nucleotide polymorphism (SNP) in the human GJA4 (Cx37) gene may serve as a prognostic marker for atherosclerosis, the leading cause of death worldwide. Atherosclerosis is a chronic inflammatory disease that results from an imbalance in lipid metabolism and a maladaptive immune response in the wall of large and medium-sized arteries. The atherogenic process is initiated by flow-mediated inflammatory changes in endothelial cells and the sub-endothelial retention of cholesterol-rich lipoproteins, resulting in the build-up of cholesterol-laden macrophages at sites of blood flow disturbance. Disease progression involves further recruitment of leukocytes and smooth muscle cells, immune reactions, deposit of extracellular matrix, the formation of a necrotic core and plaque angiogenesis. Atherosclerotic disease often remains silent for decades until endothelial erosion or rupture of the atherosclerotic plaque triggers the formation of a thrombus resulting in acute ischemic events such as myocardial infarction or stroke.

The GJA4 1019C>T SNP results in a non-conservative Proline-to-Serine substitution in the C-terminus of Cx37, which has a significant impact on channel function under basal and phosphorylating conditions. Cx37 is expressed in many arterial and atheroma-associated cell types including various endothelial and immune cells as well as platelets. Using mouse models for atherosclerosis, it has been shown that Cx37 hemichannels control the initiation of atherosclerosis by inhibiting autocrine ATP-dependent regulation of monocyte adhesion. Moreover, arterial blood flow patterns regulate Cx37 expression thereby inducing the formation of gap junction communication compartments in the endothelium and contributing to the location of the disease. Apart from these channel-dependent roles of Cx37, it is becoming increasingly clear that non-canonical functions of Cx37 such as their interactions with other proteins or transcription factor hijacking may critically regulate the function of endothelial or immune cells. In this presentation, I will discuss the diverse role of Cx37 as well as the GJA4 1019C>T SNP in atherosclerosis.

Keynote: Sunday July 28, 2019, 08:45 – 09:30

Douglas Baylis | Joseph & Frances Larner Professor & Chair of Pharmacology, University of Virginia

Douglas A. Bayliss received his PhD in Physiology from the University of North Carolina and did postdoctoral training in Physiology & Biophysics at the University of Washington before joining the

faculty of the University of Virginia, where he is now Joseph and Frances Larner Professor and Chair of Pharmacology. Dr. Bayliss has a longstanding interest in understanding cellular and ionic mechanisms that

regulate neuronal excitability in various physiological and clinical contexts. This has entailed characterizing the molecular basis for ion channels that contribute to modulation of cell function by physicochemical factors, endogenous neurochemicals and commonly used drugs. In this conference, he will discuss his laboratory’s recent work on Pannexin 1 channels – their properties, mechanisms of activation, and contributions to (patho)physiological processes.

Pannexin Channels: Properties & mechanisms of activation

Pannexin 1 (PANX1) is a widely expressed membrane ion channel that, when activated, supports transmembrane flux of nucleotides and other metabolites. In this presentation, we will consider basic features of PANX1 channels, specifically detailing characteristics and permeation properties of channels activated by C terminal proteolytic cleavage and by G protein-coupled receptor (GPCR) signaling. For cleavage-based PANX1 activation, a caspase-mediated process that is associated with programmed cell death, we review a novel stepwise activation that accompanies removal of individual C termini from the oligomeric channel. The activation sequence entails quantized changes in single channel conductance and activity that correlate remarkably well with macroscopic whole cell currents and large molecule permeation (ATP release, dye uptake). These caspase-activated channels adopt a large “pore” conformation, as judged by negative stain electron microscopy, and they attain a peak conductance of <100 pS at depolarized membrane potentials; an outwardly-rectifying unitary conductance accounts for whole cell current-voltage (I-V) properties in the absence of any discernible voltage-dependent channel gating. Channel properties were remarkably similar in a cell reconstitution system established to recapitulate α1-adrenoceptor activation of PANX1. In this context, we find evidence for channel activation by a diffusible signaling pathway initiated by Gαq, as expected for the α1D receptor, that was nevertheless independent of phospholipase C activation or raised intracellular calcium. We will present ongoing work exploring cellular mechanisms and possible posttranslational modifications that govern this GαqPCR-mediated PANX1 channel activation mechanism. Finally, we share results from recently-described PANX1 blockers, illustrating their effects on channels activated by C terminal cleavage or GαqPCR-signaling mechanisms. In sum, we will present our current thinking regarding these specific PANX1 activation mechanisms and the associated channel properties.

The work presented was performed in collaboration with a Pannexin Interest Group at the University of Virginia, and underwritten by our shared Program Project Grant (P01 HL120840).

Keynote: Monday July 29, 2019, 08:45 – 09:30




Jean X Jiang | University of Texas Health Science Center at San Antonio and Associate Director, Joint Biomedical Engineering Graduate Program of UTSA/UTHSCSA

Dr. Jean Jiang is a Professor and holds Ashbel Smith Professorship at the University Of Texas Health Science Center at San Antonio. She obtained her Ph.D. degree from Stony Brook University and

postdoctoral training at Harvard Medical School. Her research focuses on mechanistic roles of connexin-forming gap junctions and hemichannels in the lens and bone cells and in cancer bone metastasis. Her laboratory

has developed cell-based assays, animal models and specific research tools.

Connexin hemichannels in cellular hemostasis, mechanotransduction and cancer metastasis

Connexin (Cx) 43 is richly expressed in osteocytes, the most abundant bone cell type, and forms gap junctions and hemichannels. Earlier studies unveil the critical roles of Cx43 in bone remodeling and osteocyte viability in Cx43 knockout models. We further demonstrate specific role of Cx43 hemichannels in osteocytes using Cx43 transgenic models expressing dominant negative Cx43 mutants. Osteocytes are the major mechanosensory cells in the bone and Cx43 hemichannels in osteocytes are induced open by fluid flow shear stress (FFSS), the major form of mechanical stimulation applied to osteocytes in situ. Activation of hemichannels by FFSS released prostaglandins, known factors in promoting bone formation in response to mechanical loading. Integrins are mechanical sensors that convey the signals elicited by mechanical stress in many cell types, including osteocytes. We have shown that FFSS induces the activation of hemichannels in osteocytes and function of Cx43-hemichannels is regulated not only by mechanical stimulation, but also by α5β1 integrins. We further demonstrate that integrin acts as a regulator for the activation of Cx43-forming hemichannels in response to mechanical stress. This activation is mediated by PI3K signaling and active Akt directly phosphorylates C-terminus of Cx43 that enhances the direct +interaction between Cx43 and integrins. However, accumulated extracellular prostaglandins released by hemichannels act as a negative regulator, via activated MAPK and direct phosphorylation of Cx43, inactivates Cx43 hemichannels. Establishing the role(s) that integrins and downstream signaling mechanism plays in the regulation of hemichannel activities reveals a major mechanotransduction mechanism of osteocytes in response to mechanical stress and in facilitating anabolic function of the bone. The skeleton is the most preferred site for breast cancer metastasis and bone metastasis occurs in 70-80% of patients with advanced breast cancer. Our recent studies point to the specific involvement of hemichannels showing that activation of Cx43 hemichannels by either mechanical stimulation or chemical reagents in osteocytes play an important role in the suppression of breast cancer migration, growth and metastasis. These experimental evidences establish osteocytic Cx43 hemichannels as a potential drug target for treating cancer bone metastasis. We recently developed an antibody that bound Cx43 and activated Cx43 hemichannels in osteocytes both in vitro and in vivo. The treatment of this antibody suppressed human breast cancer bone metastasis and increased lifespan in mouse models. Together, these studies suggest that Cx43 hemichannels in osteocytes play crucial roles in anabolic function of bone in response to mechanical loading and in suppressing cancer bone metastasis. These channels could be a potential new therapeutic target in the treatment of bone diseases and metastasis.

Keynote: Tuesday July 30, 2019, 08:45 – 09:30

KEYNOTE PRESENTATIONS

Rob Gourdie | Director of the Center for Heart and Regenerative Medicine (CHARM) at the Fralin Biomedical Research Institute at VTC (FBRI) and Professor in the Department of Biomedical Engineering and Mechanics at Virginia Tech

Rob’s research on connexins includes studies of their role in fundamental mechanisms of cardiac bio-electricity. His lab also undertakes translational research on drugs targeting connexin function in heart

disease, wound healing and oncology. He is co-founder of FirstString Research Inc a clinical-stage biotech company, which is presently in Phase III testing of a connexin43-based therapeutic that was developed in his lab. Gourdie received his BSc from the University of Auckland. He did his PhD (1990) at the University of Canterbury (NZ) and post-doctoral training at University College London (United Kingdom), as a British Heart Foundation Fellow.

Connexin43: For better or worse, in sickness and in health…

This talk will be on the Gourdie lab’s studies of the gap junction (GJ) protein connexin 43 (Cx43) and focus on two main areas. First, our characterization of the organization and function of the Cx43 GJ perinexus in the heart will be addressed. In particular, data on how the perinexus serves as a niche for voltage-gated sodium channels and how perinexal sodium currents may contribute to a novel mechanism for electric field/ephaptic conduction of cardiac action potentials will be summarized. Second, our work on Cx43 mimetic peptides will be described. This will include a review of published studies that led to the translation of the Cx43 carboxyl terminus (CT) mimetic aCT1 to a randomized Phase III clinical trial in patients with diabetic foot ulcers, as well as highlight our unpublished data on the potential of exosome-encapsulated Cx43 CT mimetic peptide for treatment of myocardial infarction.

Keynote: Wednesday July 31, 2019, 08:45 – 09:30




AUTHOR INDEX

Name Presentation #

Aasen, Trond P-A-11, P-F-69, S7.1

Abbott, Ana C P-I-86

Abert, Christine P-C-52

Abitbol, Julia M S4.3

Acott, Ted S9.4

Acuña, Rodrigo P-A-7

Ahmad, Rafi S10.5

Akawi, Rajaa P-C-53

Alamban, Andrew John T

P-C-44

Albasini, Marion P-B-27

Allagnat, Florent P-B-26, P-B-27

Almad, Akshata S6.2

Alonso, Florian P-B-26

Altenberg, Guillermo P-D-63

Alvarado, Francisco Javier

S1.2

Name Presentation #

Álvarez, Javiera P-A-9

Amini, Azin E S4.6

An, Biao S9.5

An, Weijun P-H-72

An, Zhiqiang S7.3

Anderson, James R P-H-84

Andjelkovic, Anuska V P-C-45

Aquilino, Mark S P-D-65

Aseervatham, Jaya S5.1

Au, Akina S9.5

Bacon, Hannah S3.2

Bader, Almke S9.2

Bai, Donglin S3.6

Baillie, George S S3.1

Banks, Lawrence S8.1

Bardakjian, Berj L P-D-65

Barr, Kevin J S1.1, S4.3

Name Presentation #

Barrett, Paula Q P-B-28

Batista-Almeida, Daniela

P-C-52

Batra, Surinder S2.2

Bayik, Defne P-A-23

Bayliss, Douglas A P-B-28

Bazzigaluppi, Paolo S4.6

Bechberger, John F P-H-73, S7.5, S10.1

Begandt, Daniela S2.3, S3.2

Beier, Frank S1.1

Bennett, Steffany P-H-81

Bera, Amal Kanti P-D-59

Berthoud, Viviana M P-G-70, S5.4

Best-Campbell, Angela K

P-B-28, S2.3

Beyer, Eric C P-G-70, S5.4

Bhat, Krishna S7.4

All authors (lead and additional) and presenters are listed here for easy cross-referencing to their respective presentation. The list of full abstracts is available as a download from the IGJC website (www.igjconference.org).

Interpreting the presentation numbers:

The first section of the number represents the type of presentation as follows:

S - Oral presentation

P - Poster Session

The second section represents the session number for Oral presentations or the subject/theme for posters.

The third section indicates the order of presentation for oral presentations or poster number.

Theme Code:

A – Cancer

B – Cardiovascular

C – Cell

D – Channel structure-function/Biophysics

E – Development and reproduction

F – Emerging areas/Other

G – Inherited diseases

H – Nervous system

I – Sensory system

J – Therapeutic applications

Name Presentation #

Bhattacharya, Shashwati

S8.4

Biwer, Lauren S3.2

Blanco, Alfonso P-C-57

Bose, Abhishek P-H-74

Boyce, Andrew P-C-51

Boyce, Andrew KJ S4.4

Bravo-López, Susana B P-A-3, P-C-57

Brennand, Kristen P-H-71

Brink, Peter R P-J-89

Brown, Cherie A P-I-87

Brown, Craig E P-H-85

Bruzzaniti, Angela S9.3

Bultynck, Geert P-B-24

Burendei, Batuujin S5.2

Burt, Janis M P-B-32, P-C-41, P-C-44, P-J-88, S5.3

Busch, Karl Emanuel P-H-79, S6.1

Buxbaum, Joseph P-H-71

Caeiro, José R P-C-57

Calderon, Frances P-H-80

Calhoun, Patrick J P-B-33, S2.5

Calleja-Chuclá, Teresa P-A-3

Cannon, Andrew S2.2

Carlen, Peter L P-D-65, P-H-77, S4.6

Carrasco, Jorge P-D-61, P-D-66, S4.2

Carrasco Alarcón, Jorge

S4.2

Castro-Iglesias, Alejandro

P-A-3

Catarino, Steve P-C-42, P-C-52, S2.6

Cerrone, Marina S1.2

Chanson, Marc P-A-18

Chavez, Jeffrey B P-A-4, S7.3

Chen, Honghong S3.6

Chen, Vincent P-C-35, S10.3

Chhouri, Hussein P-A-19, P-A-20

Choi, Catherine P-H-85

Choi, Eun Ju P-C-40, S3.5

Choi, Kate P-C-38

Civitelli, Roberto S9.6

Cogliati, Bruno S6.6

Name Presentation #

Cohen, Ira S P-J-89

Comstra, H Skye S3.2

Contreras, Jorge E P-B-34, P-D-64, P-H-80, S1.4, S6.2

Coronel-Cruz, Cristina

P-C-56

Cotter, Maura L P-J-88

Cowan, Kyle N P-A-12, P-C-43, P-D-62

Cruz-Miguel, Lourdes P-C-56

Czyz, Jaroslaw P-A-1

D'Amico, Daniela P-C-48

Dahal, Jeebika P-H-84

Dakik, Hassan P-A-20

Dalal, Miloni P-H-80

Dang-Lawson, May P-C-38

Das Sarma, Jayasri P-H-74

De Bock, Marijke S6.3

De Neve, Jan P-D-58

De Smet, Maarten P-B-24, P-B-25, P-D-58, S6.3

DeBock, Marijke S10.1

Degen, Katherine E S10.4

DeLalio, Leon J P-B-28, S2.3, S3.2

Delmar, Mario P-B-25, S1.2

Deosthale, Padmini S9.3

Desai, Bimal P-B-28

Deweyert, Andrew P-A-15

Dinarès, Carme P-A-11

Dirks, Anne S9.2

Dolan, Kimberly A S4.1

Domínguez Azorín, Daniel

S7.1

Domínguez-Prieto, Marta

S7.5

Dong, Li S8.1

Donnelly, Iona S2.3

Donoso, Maribel P-C-50

Dries, Eef P-B-25

Dukic, Aleksandra S10.5

Dural, Cem P-A-12, P-D-62

Ebert, Sarah N P-C-51, P-H-85

Eill, Elizabeth G S8.6

Ek-Vitorin, Jose F P-B-32, P-J-88

El Sabban, Marwan E P-A-19, P-A-20, P-C-53

Name Presentation #

El-Hajjar, Layal P-A-20

El-Saghir, Jamal P-A-19

Elgheznawy, Amro S2.4

Eliseo, Eugenin P-C-48

Emrich, Daniel P P-H-84

Endo, Daiki P-A-2

Epp, Anna P-C-51

Errico, Carmela S9.1

Esakov, Emily L S8.2

Essex, Alyson S9.3

Eugenin, Eliseo P-C-49, P-C-50, S7.4

Falk, Matthias M P-C-36, P-C-37, P-C-90, S8.3

Fernández, Ainoa P-A-13

Fernandez, Paola P-D-60, P-H-83

Fiori, Mariana P-D-63

Fischer, Katharina Elisabeth

P-H-79, S6.1

Fisher, Charles G P-C-36, P-C-37

Flores, Jonathan A S4.1

Fonseca-Capdevila, Eduardo

P-A-3, P-C-57

Fontana, Francesca S9.6

Fortunato, Manuela S9.6

Fowler, Stephanie L P-H-81

Fraidenraich, Diego P-B-34, S1.4

Frederiksen, Simona D

P-C-54, P-E-68

Freeman, Emily P-D-62

Freitas-Andrade, Moises

P-H-73, S10.1

Fricker, Brandon S6.5

Fujiyoshi, Yoshinori S5.2

Gaete, Pablo S P-D-64

Gao, Junyuan S5.4

Gao, Ya P-C-90

Garcia, Jessica C P-H-84

Garcia-Vega, Laura M S9.1

García-Vicente, Laura S7.5

Geiger, Jonathan D P-C-49

George, Kijana K S2.5

Gibbins, Jonathan S2.4

Girão, Henrique P-B-24, P-C-42, P-C-52, S2.6

http://www.igjconference.org




Name Presentation #

Gleisner, María Alejandra

P-A-9

Gloor, Gregory B P-C-47

Gomez, Ariel R P-B-28

Gonçalves, Alexandre F

P-C-42

Gonçalves, Lino M S2.6

Good, Miranda E P-B-28, S3.2

Gordon, Chris P-J-89

Gorska, Anna M P-C-49

Gourdie, Robert G P-B-31, S1.3, S6.4, S10.4

Graham, Sheila V S8.1

Gross, Sarah S6.2

Gu, Sumin P-A-6, S7.3

Guagliardo, Nick A P-B-28

Guitián-Caamaño, Amanda

P-A-3, P-C-57

Haas, Julie S P-H-75, S6.5

Haddad, Bassam G S4.1

Haefliger, Jacques-Antoine

P-B-26, P-B-27, S3.4

Halade, Ganesh S3.3

Hamard, Lauriane P-B-26

Hammad, Ahmed P-A-12

Harris, Andrew L P-D-64

Hashem, Yasmine P-C-53

Hatakenaka, Tomohiro

P-A-16

He, Jia P-B-31

Hebb, Matthew P-A-15

Heckman, Emily S6.5

Heo, YunKyung S3.5

Hernández-Losa, Javier

P-A-11

Herrera, Carolina P-A-15

Hijazi, Rawya P-C-53

Name Presentation #

Himelman, Eric P-B-34, S1.4

Hitomi, Masahiro P-A-23

Hoagland, Daniel P-B-31

Hofmann-Vega, Francisca

P-A-9, P-A-10

Holland, Stephen P-A-12

Hong, Jung Min S9.3

Hong, Kwangseok S3.2

Horikawa, Makoto P-A-22

Hsieh, Chin-Ling P-B-29, P-B-30

Hu, Anna P-C-45

Huang, Kate P-C-35

Huang, Kenneth S8.5

Huang, Yanru P-C-46, S7.6

Huke, Sabine S3.3

Hulpiau, Paco P-B-24

Husari, Ahmad P-C-53

Im, Wonpil P-C-90

Iovine, Kathryn S8.3, S8.4

Isakson, Brant E P-B-28, S1.1, S2.3, S3.1, S3.2

Jackson, Michael F P-C-47

Jacobsen, Nicole L P-C-41, S5.3

Jadapalli, Jeevan K S3.3

Jalaleddine, Nour P-A-20

James, Carissa C P-B-33, S2.5

Jara, Oscar R P-G-70, S5.4

Jaraíz-Rodríguez, Myriam

S7.5

Jensen Flores, Joaquin P-D-61, P-D-66

Jiang, Jean X P-A-4, P-A-6, P-A-8, S7.3

Johnson, Allison M P-C-45

Johnston, Danielle P-A-15, P-C-47, S4.3, S8.5

Johnstone, Scott R P-B-28, S1.1, S2.3, S3.1, S3.2, S8.1

Name Presentation #

Jolly, Sonia S4.5

Jones, Bryan W P-H-84

Joseph, Jessica S6.2

Jourdan, Jane S1.3

Jourdan, Linda J S10.4

Jourdin, Jane P-B-31

Jurcic, Kristina P-C-47

Kajiwara, Yuji P-H-71

Kameritsch, Petra S2.1

Kamiya, Kazusaku S5.5

Kaneko, Saki P-A-2

Kato, Kazunori P-A-2

Keep, Richard F P-C-45

Keller, T.C. Stevenson P-B-28

Kelley, Mary S9.4

Kelly, John J P-A-15, S4.3

Kenmochi, Hiroaki P-A-22

Khosla, Kanika S7.2

Kim, Joonchul S1.2

King, D. Ryan P-B-31

Knollmann, Bjorn S3.3

Koizumi, Shinichiro P-A-22

Kondo, Mineo P-H-84

Kouzi, Farah P-A-19

Koval, Michael P-H-74, S2.3, S3.1, S3.2

Kruger, Nenja A S3.2

Kuntz, Nicole S9.5

Kurtz, Armin S3.4

Kwiecień, Edyta P-A-1

Laird, Dale W. S9.5

Lakhani, Zameena S8.5

Lamouille, Samy S6.4

Lampe, Paul D P-J-88, S1.6, S3.1, S10.1

Name Presentation #

Langlois, Stephanie P-A-12, P-C-43, P-D-62

Largo, Raquel P-C-57

Lathia, Justin D P-A-21, P-A-23, S8.2

Le, Thu H P-B-28

Le Gal, Loïc S3.4

Le Pham, Tammy P-D-62

Le Vasseur, Maxence P-C-35

Lee, Andrew P-B-34

Lee, Jinu P-C-40, S3.5

Lee, Seung-yon S9.6

Lee, Yi-Nan P-B-29

Lehrich, Tina S9.2

Leybaert, Luc P-B-24, P-B-25, P-D-58, S6.3, S10.1

Li, Hanjun S2.2

Li, Xinbo S9.4

Lillo, Mauricio A P-B-34, P-D-64, S1.4, S6.2

Lin, Richard Z P-J-89

Lin, Xianming P-B-25

Lin, Ya-Ping S5.1

Lin, Zhenguo S4.5

Lissoni, Alessio P-B-24, P-B-25, P-D-58

Litchfield, David W P-C-47

Liu, Mei P-H-85

Liu, Tom P-B-31

Liu, Xiaowen P-A-6

Liu, Yu P-D-64

Lizana, Marcelo P-A-10

Lohman, Alexander S3.2

Lopez, William P-D-64

Lukewich, Mark K P-D-65

Maaliki, Lama P-C-53

Macal Jr, Edgar H S2.3

Mackowiak da Fonseca, Ivone Izabel

P-A-5

Mao, Zhimin P-C-46, S7.6

Maragakis, Nicholas S6.2

Marc, Robert E P-H-84

Maripillán, Jaime P-I-86

Márquez-Rosado, Lucrecia

S1.6

Name Presentation #

Martens, Sascha P-C-52

Martin, Patricia E S9.1

Martínez, Agustín D P-I-86

Martinez-Saez, Elena P-A-11

Martins-Marques, Tania

P-B-24, P-C-42, S2.6

Masati, Ester P-B-28

Massimi, Paola S8.1

Mathias, Richard T S5.4

Matsuuchi, Linda P-C-38

Maulik, Mahua P-H-74

Mayán Santos, María Dolores

P-A-3, P-C-57

Mazzolai, Lucia S3.4

McBride, Martin S2.3

McCutcheon, Sean P-A-17

Meda, Paolo P-B-26, S3.4

Medina, José M S7.5

Mendu, Suresh P-B-28

Menetrey, Steeve P-B-27

Mersman, Brittany S4.5

Miller, Ashley M S2.3

Milstein, Jenna A P-B-28, S2.3

Minogue, Peter J P-G-70, S5.4

Mitchell, Cheryl K S5.1

Mo, Victor P-C-38

Mohamed, Hisham P-A-12

Mohsen-Kanson, Tala P-A-19

Molina, Samuel A S3.2

Moline, Teresa P-A-11

Monnier, Philippe S4.6

Montgomery, Jade S10.4

Montiel, Juan F P-H-83

Morrison, Hope P-H-84

Mukherjee, Priyanka P-H-74

Mulkearns-Hubert, Erin E

P-A-21, P-A-23, S8.2

Muñoz Guijarro, María José

P-F-69, S7.1

Muñoz-Ballester, Carmen M

S6.4

Myers, Janette B S4.1

Nagy, James S9.4

Namba, Hiroki P-A-22

Nardelli Haefliger, Denise

P-B-26

Name Presentation #

Naus, Christian P-C-35, P-H-71, P-H-73, S7.2, S7.5, S10.1

Nazarali, Samina S9.5

Neumann, Ruth S9.5

Nezlobinsky, Timur P-B-25, P-D-58

Ngezahayo, Anaclet S9.2

Nouri-Nejad, Daniel P-C-47, S8.5

O'Brien, John P-H-82, S5.1

O'Donnell, Brooke P-A-15, P-C-47, S4.3

Oishi, Tomoya P-A-22

Orellana, Viviana P-A-14

Oshima, Atsunori S5.2

Ottolini, Matteo S1.1

Padget, Rachel L P-B-33

Palacios-Prado, Nicolas

P-D-60

Panfilov, Alexander P-B-25, P-D-58

Patel, Kaushik S2.2

Patil, Chetan P-C-47

Paw, Milena P-A-1

Pearce, Chris S7.1

Peg, Vicente P-A-11

Pelaz, Sara G S7.5

Pellegrin, Maxime S3.4

Peña, Francisca P-A-14

Penuela, Silvia P-A-15, P-C-47, S1.1, S4.3, S8.5

Pérez-Acle, Tomas C P-D-61, P-D-66, S4.2

Perez-Armendariz, E. Martha

P-C-56

Perez-Hernandez, Marta

P-B-25, S1.2

Peyter, Anne-Christine

P-B-27

Pfeiffer, Rebecca L P-H-84

Pham, Dien G P-H-72

Pham, Tuan P-H-75

Phan, Allen V P-B-33

Phillips, Chelsea M P-C-45

Piotrowska, Karolina P-A-1

Pires, Inês F P-C-42

Piwowarczyk, Katarzyna

P-A-1

Plotkin, Lilian I S9.3

AUTHOR INDEX




Name Presentation #

Pogoda, Kristin S2.1

Pohl, Ulrich S2.1

Pokharel, Aayush S6.2

Pontifex, Tasha P-B-32, P-C-41, P-C-44, S5.3

Pournia, Farnaz P-C-38

Prideaux, Brendan P-C-48

Pronin, Alexey P-H-72

Purohit, Rutambhara P-D-59

Radio, Stanley S2.2

Ramón y Cajal, Santiago

P-A-11

Rapp, Christopher N P-H-84

Rapp, Kevin D P-H-84

Ravichandran, Kodi P-B-28

Reichow, Steve L S4.1

Reizes, Ofer S8.2

Reshetnikova, Galina P-H-72

Retamal, Mauricio A P-A-7, P-A-13, P-A-14, P-D-63

Reyna, Mauricio P-I-86

Ribeiro-Rodrigues, Teresa M

P-C-52, S2.6

Riquelme, Manuel A P-A-4, P-A-6, P-A-8, S7.3

Robel, Stefanie S2.5, S6.4

Romagosa, Cleofé P-A-11

Ronald, John P-A-15

Rosa-Molinar, Eduardo

P-H-82

Rothenberg, Eli P-B-25

Rovegno, Maximiliano

P-H-83

Ruddiman, Claire A P-B-28

Saez, Juan C P-D-60, P-H-83, S10.2

Safarian, Nickie P-H-77, P-H-78

Safi, Remi P-A-19

Name Presentation #

Saito, Mikako P-A-16

Saito, Toshiki P-A-16

Salazar-Onfray, Flavio P-A-9, P-A-10

Saliba, Jessica L P-A-20, P-C-53

Salzer, Grace E S3.3

Sameshima, Tetsuro P-A-22

Sánchez, Helmuth A P-I-86

Sanchez-Arias, Juan C P-C-51, P-E-68, P-H-85

Sanchez-Pupo, Rafael E

P-A-15, P-C-47, S4.3, S8.5

Sansano, Irene P-A-11

Santoro, Tania P-B-26, P-B-27, S3.4

Santos-Miranda, Artur

S3.6

Sato, Ayami P-A-5

Sayedyahossein, Samar

S8.5

Scemes, Eliana P-C-50, P-H-76

Schadt, Eric P-H-71

Schultz-Hansen, Rie P-H-73

Schulz, Rainer P-B-24

Seo, JoonHo P-H-80

Sequeira-Lopez, Maria Luisa S

P-B-28

Shah, Bindra S9.5

Shaito, Abdullah P-A-20, P-C-53

Shandra, Oleksii S6.4

Shao, Qing Cindy C S9.5

Shestopalov, Valery M P-H-72

Shevtsova, Olga P-E-68

Shinozaki, Ruriko S5.2

Shirokova, Natalia S1.4

Shropshire, Daniel B P-A-4

Shu, Xiaohong S2.3

Sidhu, Harry S9.3

Name Presentation #

Sigulinsky, Crystal L P-H-84

Silver, Daniel J P-A-23

Sin, Wun Chey P-H-71, S7.2, S7.5

Sipido, Karin P-B-25, P-D-58

Slepak, Vladlen Z P-H-72

Smyth, James W P-B-33, S2.5, S3.1, S6.4

Sośniak, Justyna P-A-1

Sofoluwe, Aderonke P-A-18

Solan, Joell L P-J-88, S1.6

Sonkusare, Swapnil P-B-28, S1.1, S3.2

Sorgen, Paul L P-C-55, S2.2

Soto, Paola P-D-60

Spagnol, Gaelle P-C-55, S2.2

Spray, David P-A-17

Spurlock, Marcus P-H-72

St-Pierre, Marie-Eve P-A-12, P-D-62

Stefanovic, Bojana S4.6

Stehberg, Jimmy P-D-63

Stevenson, Andrew S8.1

Strauss, Randy S1.3

Su, Cheng-Huang P-B-29, P-B-30

Su, Yeu P-B-29, P-B-30

Swayne, Leigh Anne P-C-51, P-C-54, P-E-68, P-H-85

Tabernero, Arantxa S7.5

Taetzsch, Thomas S2.5

Taffet, Steven M S8.6

Taga, Arens S6.2

Talaverón, Rocío S7.5

Tani, Kazutoshi S5.2

Tasken, Kjetil S10.5

Taskina, Daria P-H-77

Taylor, Graeme P-H-81

Taylor, Jordan D P-B-33

Taylor, Samantha P-C-41, S5.3

Name Presentation #

Temprana, Jordi P-A-11

Terada, Tohru S5.2

Terasaki, Hiroko P-H-84

Thiagarajan, Praveena S8.2

Thomas, Deepthi P-H-74

Thompson, Shannon M

P-H-81

Thomspon, Roger J S4.4

Tian, Yi P-A-6, P-A-8

Tichauer, Juan E P-H-83

Tien, Ting-Yi P-B-29, P-B-30

Tishchenko, Alexander

S7.1

Tittarelli, Andres P-A-9, P-A-10

Tokunaga, Naruwa P-A-16

Torre-Healy, Luke A P-A-23

Trease, Andrew J P-C-55, S2.2

Tuana, Balwant S1.5

Vagner, Josef P-J-88

Valdebenito, Silvana S7.4

Valdez, Gregorio S2.5

Valdivia, Hector S1.2

Valiunas, Virgis P-J-89

Valiuniene, Laima P-J-89

Van Haver, Valerie S6.3

Van Pel, Derek S7.2

Vandenbroucke, Roosmarijn E

S6.3

Vandersickel, Nele P-D-58

Varela Vázquez, Adrián

P-A-3, P-C-57

Varela-Eirín, Marta P-C-57

Vargas, Anibal A P-H-83

Velisek, Libor P-H-76

Veliskova, Jana P-H-76

Verwaerde, Stijn S6.3

Vidal-Brime, Laia S7.1

Villanelo, Felipe P-D-61, P-D-66, S4.2

Vinken, Mathieu S6.6

Vladis, Nathalie S6.1

Vogl, Wayne A P-C-35

Wagner, Charlotte S3.4

Wakefield, Charles B S4.3

Walzl, Dennis P-H-79

Wang, Erming P-H-71

Name Presentation #

Wang, Helen Y S5.1

Wang, Hsueh-Hsiao P-B-30

Wang, Jasmine S P-H-73

Wang, Minghui P-H-71

Wang, Nan P-B-24, P-B-25, P-D-58, S10.1

Watanabe, Masakatsu P-E-67, S5.2

Watkins, Marcus S9.6

Watt, Carl B P-H-84

Weaver, Rachel B P-B-28

White, Kyra S9.5

Whitehead, Shawn N P-H-73

Whyte - Fagundes, Paige A

P-D-65, P-H-77, P-H-78

Wicki-Stordeur, Leigh E

P-C-51, P-C-54

Witschas, Katja P-B-24, P-B-25, P-D-58

Wolpe, Abigail G S1.1

Wu, Yih-Jer P-B-29, P-B-30

Xiang, Xiao P-C-43

Xie, Lai-Hua P-B-34, S1.4

Xu, Fenglian S4.5

Xu, Hu P-A-15

Xue, Mengzhou S9.4

Yamamoto, Taisuke P-A-22

Yamasaki, Tomohiro P-A-22

Yamazaki, Takumi P-A-2

Yang, Jia-Hui P-H-84

Yang, Xiawen S7.6

Yang, Yang S2.3, S3.1

Yano, Tomohiro P-A-2

Yao, Jian P-C-46, S7.6

Yeh, Elizabeth P-A-5

Yeh, Hung-I P-B-29, P-B-30

Yeo, Joo Hye P-C-39

Yeung, Ken K P-H-73

Yoshioka, Craig C S4.1

Zaidan Dagli, Maria Lucia

P-A-5

Zeitz, Michael J P-B-33, S2.5

Zhang, Bin P-H-71

Zhang, Fengyan S9.4

Zhang, Liang P-D-65

Zhang, Mingliang S1.2

Zhang, Xiling P-C-46

Name Presentation #

Zhao, Hong-Bo S5.6

Zheng, Hong S2.2

Zheng, Li P-C-55, S2.2

Zhou, Lufang S3.3

Zibara, Kazem P-A-19, P-A-20

Zoidl, Cristiane P-H-77, P-H-78

Zoidl, Georg P-D-65, P-H-77, P-H-78, P-I-87, S4.6

Zuckerman, Daniel M S4.1

AUTHOR INDEX




POSTER SESSION

A - CANCERP-A-1 The inhibitory effect of fenofibrate on Cx43-dependent diapedesis of cancer cellsJarosław Czyż¹, Milena Paw¹, Justyna Sośniak¹, Edyta Kwiecień¹, Karolina Piotrowska¹, Katarzyna Piwowarczyk¹

¹Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University

P-A-2 A new preventive strategy for targeting gap junction in prostate cancer stem cellsDaiki Endo¹, Tomohiro Yano¹, Saki Kaneko¹, Kazunori Kato¹, Takumi Yamazaki¹

¹Toyo University

P-A-3 Non-SUMOylated Cx43 changes the function and recruitment of cellular components into exosomes and prevents drug resistance to BRAF/MEK inhibitors in metastatic melanomaAdrián Varela Vázquez¹, Amanda Guitián-Caamaño², Alejandro Castro-Iglesias¹, Susana B. Bravo³, Teresa Calleja-Chuclá⁴, Eduardo Fonseca-Capdevila⁵, María Mayán¹

¹Instituto de Investigación Biomédica de A Coruña (INIBIC). Servizo Galego de Saúde (SERGAS). Univers, ²Instituto de Investigación Biomédica de A Coruña (INIBIC), ³Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Univers, ⁴Hospital Teresa Herrera. Servicio Galego de Saúde (SERGAS)., ⁵Hospital Abente y Lago. Servivio Galego de Saúde (SERGAS)

P-A-4 Suppression of Osteosarcoma progression by ATP and Bone Osteocyte Connexin 43 Hemichannel activityDaniel Shropshire¹, Manuel Riquelme¹, Jeffrey Chavez¹, Jean Jiang¹

¹UT Health Science Center San Antonio

P-A-5 a-Connexin carboxyl-terminal peptide enhances antiproliferative effect of Bowman-Birk inhibitor from soybeans in canine oral melanoma cellsAyami Sato¹, Ivone Izabel Mackowiak da Fonseca¹, Elizabeth Yeh², Maria Lucia Zaidan Dagli¹

¹University of Sao Paulo, ²Medical University of South Carolina

P-A-6 ATP released by mechanical loading inhibits breast cancer cell migration through down-regulation of CXCR4Xiaowen Liu¹, Manuel Riquelme1, Yi Tian¹, Sumin Gu1, Jean Jiang1

¹ UT Health Science Center San Antonio

P-A-7 The moonlight role of Cx46 in the local and long-distance communication in breast cancer cellsRodrigo Acuna¹, Mauricio Retamal¹

¹Universidad del Desarrollo

P-A-8 The role of Osteocytic Connexin 43 Channels and oxidative stress in breast cancer growth and bone metastasisYi Tian¹, Manuel Riquelme1, Jean Jiang1

¹UT Health Science Center San Antonio

P-A-9 Cx43-mediated gap junction intercellular communications participate in the cytotoxic immunological synapse enabling tumor killing by CTLFrancisca Hofmann¹, Javiera Álvarez¹, María Alejandra Gleisner¹, Flavio Salazar-Onfray¹, Andres Tittarelli¹

¹Universidad de Chile, Institute of Biomedical Sciences

P-A-10 Hypoxia-induced microRNAs are transfer from melanoma to immune cells by Cx43 channelsFrancisca Hofmann¹, Marcelo Lizana¹, Flavio Salazar-Onfray¹, Andres Tittarelli¹

¹Universidad de Chile, Institute of Biomedical Sciences

P-A-11 The association between Pannexin 1 expression and cancer prognosis: an exploratory approachCleofé Romagosa¹, Carme Dinarès¹, Irene Sansano¹, Jordi Temprana¹, Vicente Peg¹, Elena Martinez-Saez¹, Teresa Moline¹, Javier Hernández-Losa¹, Santiago Ramón y Cajal¹, Trond Aasen¹

¹Vall d'Hebron Research Institute (VHIR)

P-A-12 Inhibition of human neuroblastoma growth by Probenecid and CarbenoxoloneMarie-Eve St-Pierre¹, Stephen Holland1, Hisham Mohamed1, Ahmed Hammad1, Cem Dural1, Stéphanie Langlois1, Kyle Cowan1

¹Children's Hospital of Eastern Ontario (CHEO) Research Institute, University of Ottawa

P-A-13 Over-expression of Connexin 46 in cancer cells as a facilitator of tumor progressionAinoa Fernández¹, Mauricio Retamal¹


P-A-14 Cx46 regulates HIF-1A levels in melanoma cellsViviana Orellana¹, Francisca Peña¹, Mauricio Retamal¹


P-A-15 Pannexin 1 is upregulated in patient derived glioblastoma multiforme cells and its inhibition can impair cancer cell viability and tumor formationDanielle Johnston¹, John Kelly¹, Rafael Sanchez-Pupo¹, Brooke O'Donnell¹, Carolina Herrera¹, Andrew Deweyert¹, Hu Xu¹, Matthew Hebb¹, John Ronald¹, Silvia Penuela¹

¹University of Western Ontario

P-A-16 Effect of Cx45 overexpression on metastatic potential of mouse melanoma cellsMikako Saito¹, Toshiki Saito¹, Tomohiro Hatakenaka¹, Naruwa Tokunaga¹

¹Tokyo University of Agriculture and Technology

P-A-17 A role for Connexin 43-mediated miRNA transfer in glioblastoma invasionSean McCutcheon¹, David Spray¹

¹Albert Einstein College of Medicine

P-A-18 Intercellular communication ameliorates antiviral response in polarized airway epitheliumAderonke Sofoluwe¹, Marc Chanson¹

¹University of Geneva

P-A-19 Heterocellular adhesion and communication reciprocally modulate mesenchymal stem cells in hematopoiesis and in LeukemiaTala Mohsen-Kanson¹, Remi Safi², Farah Kouzi³, Jamal El-Saghir², Hussein Chhouri⁴, Kazem Zibara¹, Marwan El Sabban²

¹Lebanese University, ²American University of Beirut, ³University of Tours, ⁴Normandie University

P-A-20 Pannexin1 is associated with enhanced epithelial-to-mesenchymal transition in human patient breast cancer tissues and in MDA-MB-231 breast cancer cellsNour Jalaleddine¹, Hassan Dakik², Abdullah Shaito³, Jessica Saliba⁴, Layal El-Hajjar¹, Hussein Chhouri⁵, Kazem Zibara⁴, Marwan El-Sabban⁶

¹Beirut Arab University, ²University of Tours, ³Lebanese International University, ⁴Lebanese University, ⁵Normandie University, ⁶American University of Beirut

P-A-21 Identifying differential intracellular interactions between Cx46 and Cx43 in glioblastoma cancer stem cellsErin Mulkearns-Hubert¹, Justin Lathia¹

¹Cleveland Clinic Foundation

P-A-22 Ivestigations for nicotinic impact on stem cells' property for HSV-tk/GCV therapy against gliomaHiroaki Kenmochi¹, Tomohiro Yamasaki², Tomoya Oishi², Makoto Horikawa², Taisuke Yamamoto², Shinichiro Koizumi², Tetsuro Sameshima², Hiroki Namba²

¹Seirei Mikatahara General Hospital, ²Hamamatsu University School of Medicine

P-A-23 Development of a Cx46 targeting strategy for cancer stem cellsErin Mulkearns-Hubert¹, Luke Torre-Healy², Daniel Silver², Defne Bayik², Masahiro Hitomi², Justin Lathia¹

¹Cleveland Clinic Foundation, ²Cleveland Clinic

B - CARDIOVASCULARP-B-24 Cx43 hemichannel intracellular Ca2+/RyR2-dependent activation in cardiomyocytesAlessio Lissoni¹, Paco Hulpiau², Tania Martins-Marques³, Nan Wang⁴, Geert Bultynck⁵, Rainer Schulz⁶, Katja Witschas¹, Henrique Girão³, Maarten De Smet¹, Luc Leybaert¹

¹Ghent University, ²HOWEST University of Applied Sciences, ³Faculty of Medicine, University of Coimbra, ⁴University of Bristol, ⁵KU Leuven, ⁶JustusLiebig Universität Giessen

P-B-25 Functional Cx43 hemichannel-dyadic nanodomains at the intercalated disc impact cardiomyocyte excitabilityMaarten De Smet¹, Alessio Lissoni¹, Timur Nezlobinsky¹, Nan Wang¹, Eef Dries², Marta Perez-Hernandez³, Xianming Lin³, Katja Witschas¹, Eli Rothenberg³, Alexander Panfilov¹, Mario Delmar³, Karin Sipido², Luc Leybaert¹

¹Ghent University, ²KU Leuven, ³New York University School of Medicine

P-B-26 Targeting Connexin37 alters angiogenesis and vessel maturation in the developing mouse retinaLauriane Hamard¹, Tania Santoro¹, Florent Allagnat¹, Paolo Meda², Denise Nardelli Haefliger¹, Florian Alonso³, Jacques-Antoine Haefliger¹

¹CHUV, ²University of Geneva, ³Université de Bordeaux

P-B-27 Participation of Connexin37 in the control of blood pressure and vessel functionMarion Albasini¹, Tania Santoro¹, Steeve Menetrey¹, Anne-Christine Peyter¹, Florent Allagnat¹, Jacques-Antoine Haefliger¹

¹CHUV




P-B-28 Pannexin 1 channels in renin-expressing cells regulate renin secretion and blood pressure homeostasisLeon DeLalio¹, Miranda Good¹, Ester Masati², Suresh Mendu², Claire Ruddiman², Scott Johnstone¹, Jenna Milstein², T.C. Stevenson Keller², Rachel Weaver², Nick Guagliardo², Angela Best-Campbell², Kodi Ravichandran², Douglas Bayliss², Maria-Luisa Sequeira-Lopez2, Swapnil Sonkusare1, Bimal Desai2, Paula Barrett2, Thu Le2, Ariel Gomez2, Brant Isakson1

¹University of Virginia School of Medicine, ²University of Virginia

P-B-29 Targeting pannexins to rejuvenate senescent EPCs for cell therapyTing-Yi Tien¹, Yih-Jer Wu¹, Cheng-Huang Su², Yi-Nan Lee2, Chin-Ling Hsieh2, Yeu Su3, Hung-I Yeh²

¹Mackay Medical College, ²Mackay Memorial Hospital, 3National Yang-Ming University

P-B-30 Connexin43 down-regulation attenuates paracrine effects of human smooth muscle progenitor cells through inactivation of NF-kappa B and AktHung-I Yeh¹, Ting-Yi Tien², Cheng-Huang Su¹, Hsueh-Hsiao Wang², Chin-Ling Hsieh¹, Yeu Su³, Yih-Jer Wu²

¹Mackay Memorial Hospital, ²Mackay Medical College, ³National Yang-Ming University

P-B-31 Analysis of Connexin-43 (Cx43) and Sodium channel colocalization at the post-infarct injury border zoneTom Liu¹, Jane Jourdin², D. Ryan King², Daniel Hoagland², Jia He³, Robert Gourdie²

¹Virginia Tech Carilion School of Medicine, ²Fralin Biomedical Research Institute, ³Virginia Polytechnic Institute

P-B-32 A mutant mimicking an ischemic preconditioned phospho-form of Cx43 lacks Vj-gatingJose Ek-Vitorin¹, Tasha Pontifex¹, Janis Burt¹

¹University of Arizona

P-B-33 Arrhythmogenic subversion of cardiac gap junctions during adenoviral infectionPatrick Calhoun¹, Jordan Taylor², Carissa James¹, Michael Zeitz¹, Rachel Padget³, Allen Phan², James Smyth¹

¹Fralin Biomedical Research Institute at Virginia Tech, ²Virginia Tech Carilion School of Medicine, ³Virginia State University and Polytechnic Institute

P-B-34 Opening of remodeled and hypophosphorylated Connexin43 hemichannels is sufficient to promote arrhythmias upon cardiac stress.Mauricio Lillo¹, Andrew Lee¹, Eric Himelman¹, Lai-Hua Xie¹, Diego Fraidenraich¹, Jorge Contreras¹

¹Rutgers University

C – CELL BIOLOGYP-C-35 Pannexin 2 localizes at ER-mitochondria contact sitesMaxence Le Vasseur¹, Vincent Chen², Kate Huang¹, Wayne Vogl¹, Christian Naus¹

¹University of British Columbia, ²Brandon University

P-C-36 Endocytosis across the connexin protein familyCharles Fisher¹, Matthias Falk¹

¹Lehigh University

P-C-37 Cx36 endocytosisCharles Fisher¹, Matthias Falk¹

¹Lehigh University

P-C-38 The Connexin43 Carboxyl Tail influences cytoskeleton dependent processes in B-LymphocytesFarnaz Pournia¹, Kate Choi¹, May Dang-Lawson¹, Victor Mo¹, Linda Matsuuchi¹

¹University of British Columbia

P-C-39 An anti-migraine agent, flunarizine, inhibits gap junctional intercellular communicationJoo Hye Yeo¹

¹Yonsei University

P-C-40 Development of GJIC assay system from cells with endogenous expression of Cx45Eun Ju Choi¹, Jinu Lee¹

¹Yonsei University

P-C-41 Connexin 37 growth suppressive phenotype is regulated in a site-specific, phosphorylation-dependent mannerSamantha Taylor¹, Tasha Pontifex¹, Nicole Jacobsen¹, Janis Burt¹

¹University of Arizona

P-C-42 EHD1 promotes lateralization of the gap junction protein Cx43 in the ischemic heartTania Martins-Marques¹, Steve Catarino¹, Alexandre Gonçalves², Inês F Pires², Henrique Girão¹

¹Faculty of Medicine, University of Coimbra, ²Faculty of Medicine, University of Porto

P-C-43 BioID as a novel tool for studying the Pannexin 1 interactomeXiao Xiang¹, Stéphanie Langlois², Kyle Cowan2

¹University of Ottawa, 2Children's Hospital of Eastern Ontario (CHEO) Research Institute, University of Ottawa

P-C-44 Cx37-13k fails to reproduce phenotypic effects of Cx37 in rat insulinoma cells but alters Cx37’s hemichannel function when co-expressedAndrew John Alamban¹, Margaret Fye1,Tasha Pontifex1, Janis Burt1

¹The University of Arizona

P-C-45 Connexin-43 isoform, 20-Cx43, induces brain endothelial barrier permeabilityChelsea Phillips¹, Allison Johnson¹, Anna Hu¹, Richard Keep¹, Anuska Andjelkovic¹

¹University of Michigan

P-C-46 The pivotal role of TRPV4 and Connexin43 channel transactivation in mediation of aminoglycoside-elicited renal tubular cell injuryXiling Zhang¹, Zhimin Mao², Yanru Huang², Jian Yao²

¹The 4th affilliated hospital of China Medical University, ²Interdisciplinary Graduate School of Medicine, University of Yamanashi

P-C-47 A novel phosphorylation site present in ancestral and derived Pannexin 1 variants regulates glycosylation, trafficking and cell viabilityDaniel Nouri-Nejad¹, Brooke O'Donnell¹, Chetan Patil², Rafael Sanchez-Pupo¹, Danielle Johnston¹, Kristina Jurcic¹, David Litchfield¹, Michael Jackson², Gregory Gloor¹, Silvia Penuela¹

¹University of Western Ontario, ²University of Manitoba

P-C-48 The role of Connexin43 containing gap junctions and unopposed hemichannels in HIV CNS damage mediated by lipidsDaniela D'Amico¹, Brendan Prideaux¹, Eugenin Eliseo¹

¹University of Texas Medical Branch

P-C-49 ATP is a key regulator of astrocytic Gap Junction channels in NeuroHIVAnna Gorska¹, Jonathan Geiger², Eliseo Eugenin¹

¹University of Texas Medical Branch, ²University of North Dakota School of Medicine and Health Sciences

P-C-50 HIV infection and meth abuse result in glial-neuronal dysfunction and CNS compromiseMaribel Donoso¹, Eliana Scemes², Eliseo Eugenin1

¹University of Texas Medical Branch, ²New York Medical College

P-C-51 A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localizationSarah Ebert¹, Anna Epp¹, Juan Sanchez-Arias¹, Leigh Wicki-Stordeur¹, Andrew Boyce¹, Leigh Anne Swayne¹

¹University of Victoria

P-C-52 Direct interaction with LC3 modulates Cx43 degradation in endothelial cellsTeresa Ribeiro-Rodrigues¹, Steve Catarino¹, Daniela Batista-Almeida¹, Christine Abert², Sascha Martens², Henrique Girão¹

¹Faculty of Medicine, University of Coimbra, ²University of Vienna

P-C-53 Mesenchymal stem cells exposed to e-cigarette smoke recover Connexin 43 expression and osteogenic differentiation potential following smoke cessationJessica Saliba¹, Abdullah Shaito², Rawya Hijazi¹, Rajaa Akawi¹, Lama Maaliki³, Yasmine Hashem³, Ahmad Husari³, Marwan El-Sabban³

¹Lebanese University, ²Lebanese International University, ³American University of Beirut

P-C-54 A novel interactome analysis of Panx1 in neural cellsSimona Frederiksen¹, Leigh Wicki-Stordeur¹, Leigh Anne Swayne¹


P-C-55 Differential action of Src on Cx45 and Cx43 mediated through binding partnersGaelle Spagnol¹, Andrew Trease¹, Li Zheng¹, Paul Sorgen¹

¹University of Nebraska Medical Center

P-C-56 Mouse pancreatic beta cells express connexin 30.2 during whole postnatal pancreatic developmentCristina Coronel-Cruz¹, Lourdes Cruz-Miguel¹, E. Martha Perez-Armendariz¹

¹Universidad Nacional Autónoma de México

POSTER SESSION




P-C-57 Connexin43-positive exosomes efficiently induce cellular senescence via p53/p16(INK4A) in target cells contributing to osteoarthritis progressionMarta Varela-Eirín¹, Adrián Varela-Vázquez¹, Amanda Guitián-Caamaño1, Susana Bravo-López2, Alfonso Blanco3, Raquel Largo⁴, Eduardo Fonseca¹, José Caeiro⁵, María Mayán1

¹Instituto de Investigación Biomédica de A Coruña (INIBIC), 2IDIS, 3UCD Conway Institute, 4Rheumatology Department, 5Complexo Hospitalario Universitario de Santiago de Compostela (CHUS)

P-C-90 Modeling structural changes associated with Cx43-CT phosphorylation: evidence for phosphorylation as a signal for gap junction internalizationMatthias Falk1, Ya Gao1, Wonpil Im1

1Lehigh University

D – CHANNEL STRUCTURE-FUNCTION/BIOPHYSICSP-D-58 The role of C-terminus in voltage-dependent Cx43 hemichannel gating, a systematic analysis by a graphical-user interfaceKatja Witschas¹, Nele Vandersickel¹, Nan Wang¹, Alessio Lissoni¹, Maarten De Smet¹, Timur Nezlobinskii¹, Jan De Neve¹, Alexander Panfilov¹, Karin Sipido², Luc Leybaert¹

¹Ghent University, ²KU Leuven

P-D-59 Pannexin1 attenuates P2X7 receptor mediated Calcium influxRutambhara Purohit¹, Amal Kanti Bera¹

¹Indian Institute of Technology, Madras

P-D-60 Functional gap junction channels formed by endogenous Pannexin1 proteins show unique biophysical propertiesNicolas Palacios-Prado¹, Paola Fernandez¹, Paola Soto¹, Juan Carlos Saez¹

¹Pontificia Universidad Catolica de Chile

P-D-61 Changes on ion conductivity caused by IC-pocket mutation in hCx26 hemichannelJoaquin Jensen Flores¹, Felipe Villanelo², Jorge Carrasco¹, Tomas Pérez-Acle²

¹Fundacion Ciencia & Vida, ²Fundacion Ciencia & Vida - Centro Interdisciplinario de Neurociencia de Valparaíso

P-D-62 Role of Pannexin 1 channels in satellite cells and skeletal muscle regenerationEmily Freeman¹, Tammy Le Pham¹, Cem Dural1, Marie-Eve St-Pierre¹, Stéphanie Langlois¹, Kyle Cowan1

¹ Children's Hospital of Eastern Ontario (CHEO); University of Ottawa,

P-D-63 4-Hydroxynonenal-induced Cx46 hemichannel inhibition through its carbonylationMauricio Retamal¹, Mariana Fiori², Jimmy Stehberg³, Guillermo Altenberg²

¹Universidad del Desarrollo, ²Texas Tech University Health Sciences Center, ³Universidad Andres Bello

P-D-64 Novel biophysical properties of molecular transport in connexin, pannexin and CALHM channelsPablo Gaete¹, Mauricio Lillo¹, William Lopez¹, Yu Liu¹, Andrew Harris¹, Jorge Contreras¹


P-D-65 Electrophysiological and behavioral charactertization of seizure following pannexin-1 blockade or genetic knockout in miceMark Aquilino¹, Paige Whyte - Fagundes², Mark Lukewich¹, Liang Zhang³, Berj Bardakjian¹, Georg Zoidl², Peter Carlen¹

¹University of Toronto, ²York University, ³University Health Network

P-D-66 Dissecting the role of the solvent for the functional activity of connexin hemichannelsFelipe Villanelo¹, Jorge Carrasco¹, Joaquin Jensen Flores¹, Tomas Pérez-Acle²

¹Fundacion Ciencia & Vida, ²Fundacion Ciencia & Vida - Centro Interdisciplinario de Neurociencia de Valparaíso

E – DEVELOPMENT AND REPRODUCTIONP-E-67 Gap junction network for stripe pattern formation of zebrafishMasakatsu Watanabe¹

¹Osaka University

P-E-68 Panx1 regulates Parvalbumin-positive interneurons in the murine postnatal cerebral cortex: Preliminary findingsOlga Shevtsova¹, Simona Frederiksen¹, Juan Sanchez-Arias¹, Leigh Anne Swayne¹


F – EMERGING AREAS/OTHERP-F-69 Direct translation of truncated connexin proteins: More than one mechanism, more than one connexinMaría José Muñoz Guijarro¹, Trond Aasen¹

¹Vall d'Hebron Research Institute (VHIR)

G – INHERITED DISEASESP-G-70 p62/SQSTM1 and cataracts caused by a connexin50 mutantOscar Jara¹, Peter Minogue¹, Viviana Berthoud¹, Eric Beyer¹

¹University of Chicago

H – NERVOUS SYSTEMP-H-71 GJA1 (connexin43) is a key regulator of Alzheimer's disease pathogenesisYuji Kajiwara¹, Erming Wang¹, Minghui Wang¹, Wun Chey Sin², Kristen Brennand¹, Eric Schadt¹, Christian Naus², Joseph Buxbaum¹, Bin Zhang¹

¹Icahn School of Medicine at Mount Sinai, ²University of British Columbia

P-H-72 Pannexin1 facilitates retinal ganglion cells loss via inflammasome and pyroptosisValery Shestopalov¹, Weijun An¹, Dien Pham¹, Alexey Pronin¹, Galina Reshetnikova¹, Marcus Spurlock¹, Vladlen Slepak¹

¹Bascom Palmer Eye Institute University of Miami School of Medicine

P-H-73 Danegaptide treatment following a novel ischemic/reperfusion stroke model in mice reduces cell death by increasing astrocytic gap junctional couplingJohn Bechberger¹, Moises Freitas-Andrade¹, Jasmine Wang², Ken Yeung2, Shawn Whitehead2, Rie Schultz-Hansen3, Christian Naus1

¹University of British Columbia, ²Western University, 3Zealand Pharma A/S

P-H-74 Mouse Hepatitis virus impairs Connexin43-mediated gap junctional intercellular communication between astrocytes by disrupting ERp29, a PDI family molecular chaperoneAbhishek Bose¹, Deepthi Thomas¹, Priyanka Mukherjee¹, Mahua Maulik¹, Michael Koval², Jayasri Das Sarma¹

¹Indian Institute of Science Education and Research Kolkata, ²Emory University School of Medicine

P-H-75 What role do electical synapses play in neural circuits?Tuan Pham¹, Julie Haas¹

¹Lehigh University

P-H-76 Astrocyte and neuronal pannexin1 differently affect seizure progressionEliana Scemes¹, Libor Velisek¹, Jana Veliskova¹

¹New York Medical College

P-H-77 Investigating a seizure mechanism in Panx1 knockout zebrafishPaige Whyte - Fagundes¹, Daria Taskina¹, Nickie Safarian¹, Cristiane Zoidl¹, Peter Carlen², Georg Zoidl¹

¹York University, ²Krembil Research Institute

P-H-78 Pannexin1 channels and dopamine receptor signaling; old players and new prospectsNickie Safarian¹, Paige Whyte - Fagundes¹, Cristiane Zoidl¹, Georg Zoidl¹

¹York University

P-H-79 Plasticity of electrical coupling in a sensory neural circuit contributes to modulation of behaviour by environmental contextKatharina Elisabeth Fischer¹, Dennis Walzl¹, Karl Emanuel Busch¹

¹University of Edinburgh

P-H-80 Myeloid Pannexin-1 channels promote neuroinflammation after traumatic brain injuryJoonHo Seo¹, Miloni Dalal¹, Frances Calderon¹, Jorge Contreras¹


P-H-81 Role of Cx30 in neurogenesis and hippocampal functionShannon Thompson¹, Stephanie Fowler¹, Graeme Taylor¹, Steffany Bennett¹

¹University of Ottawa

P-H-82 Diversity and loss of diversity in connexins used as electrical synapsesJohn O'Brien¹, Eduardo Rosa-Molinar²

¹University of Texas Health Science Center at Houston, ²University of Kansas

POSTER SESSION




P-H-83 Inhibition of connexin hemichannels and Pannexin1 channels decreases cortical spreading depression phenomena and changes on transcriptomic profile studied in brain slices.Anibal Vargas¹, Juan Tichauer², Paola Fernandez³, Juan Sáez³, Juan Montiel¹, Maximiliano Rovegno²

¹Universidad de O'Higgins, ²Pontificia Universidad Católica de Chile, ³Universidad de Valparaíso

P-H-84 Coupling architecture of the retinal Aii/ON cone bipolar cell network and alteration in degenerationCrystal Sigulinsky¹, Rebecca Pfeiffer¹, James Anderson¹, Christopher Rapp¹, Jeebika Dahal¹, Jessica Garcia¹, Jia-Hui Yang¹, Daniel Emrich¹, Hope Morrison¹, Kevin Rapp¹, Carl Watt¹, Mineo Kondo², Hiroko Terasaki³, Robert Marc¹, Bryan Jones¹

¹Moran Eye Center, University of Utah, ²Mie University, ³Nagoya University

P-H-85 Pannexin 1 regulates neuronal networks and dendritic spine formation in cortical neuronsJuan Sanchez-Arias¹, Mei Liu¹, Catherine Choi¹, Sarah Ebert¹, Craig Brown¹, Leigh Anne Swayne¹


I – SENSORY SYSTEMP-I-86 Formation of heteromeric Cx26S17F/Cx30 channels in syndromic deafness and its functional consequences on channel function and purinergic responsesAna Abbott¹, Jaime Maripillán¹, Mauricio Reyna¹, Helmuth Sánchez¹, Agustín Martínez¹

¹Universidad de Valparaíso

P-I-87 Sensorimotor behavior in the Connexin-35b (Cx35b) knock-out Zebrafish (Danio Rerio)Cherie Brown¹, Georg Zoidl¹

¹York University

J – THERAPEUTIC APPLICATIONSP-J-88 The lipidated connexin mimetic peptide, SRPTEKT-Hdc, is a potent inhibitor of Cx43 channels with specificity for the pS368 phospho-isoformMaura Cotter¹, Maura Cotter¹, Paul Lampe², Joell Solan², Josef Vagner¹, Jose Ek-Vitorin¹, Janis Burt¹

¹University of Arizona, ²Fred Hutchinson Cancer Research Center

P-J-89 Cx43 gap junctions are essential for cellular delivery of therapeutic oligonucleotidesVirgis Valiunas¹, Chris Gordon¹, Laima Valiuniene¹, Richard Lin¹, Ira Cohen¹, Peter Brink¹

¹Stony Brook University

IGJC SPONSORS

BOXONE LABSMontreal, Canada

BoxOne Labs is a private research and investment company that uses multiple strategies including early stage venture investing in fields such as applied AI, biotech, medtech and food/agritech. We're building a high-performance culture based on intellectual curiosity, honesty, humility and a continuous drive towards improvement and discovery.

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FIRSTSTRING RESEARCH, INC.Mount Pleasant, SC, USA

FirstString Research delivers clinically relevant solutions for diseases with unmet needs associated with dysregulation of inflammatory processes and an impaired injury response. FirstString’s lead technologies are connexin-mimetic peptides (α-Connexin Carboxyl-Terminal; aCT peptides) that promote the healing of acute and full-thickness wounds by mitigating excessive inflammatory responses, stabilizing cell-cell contacts, and coordinating cellular communication, ultimately serving to encourage an improved injury response. FirstString is currently advancing the clinical development of the Connexin43 mimetic peptide, aCT1, through a topical formulation, Granexin® gel. The clinical potential of Granexin® has been validated in multiple clinical trials and is in late-stage development for diabetic foot ulcers, venous leg ulcers and acute wounds.

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Theranexus is a biopharmaceutical company that develops drug candidates to be used in the treatment of central nervous system (CNS) disorders. Theranexus' unique, patented technology is aimed at increasing the efficacy of CNS drugs that have already been approved and are available on the market. Theranexus' technology is protected, unique, scalable and versatile, and used to generate high value-added proprietary drug candidates for different conditions, while reducing time-to-market and market entry costs and risks in comparison with standard drug development processes.

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POSTER SESSIONS 1 & 2

A Cancer

B Cardiovascular

C Cell

D Channel structure-function/Biophysics

E Development and reproduction

F Emerging areas/Other

G Inherited diseases

H Nervous system

I Sensory system

J Therapeutic applications

A1

A7

C47

C53

B24

B30

F69

H75A18

E68

D63

C46

C40

A3

A9

C49

C55

B26

B32

H71

H77

A22

A16 D61

C44

C38

J88

H84

A5

A11

C51

C57C90

B28

B34

H73

H79

A20

A14

D65

D59

C42

C36

I-87

H82

A2

A8

C48

C54

B25

B31

G70

H76

A23

A17

E67

D62

C45

C39

J89

H85

A4

A10

C50

C56

B27

B33

H72

H78

A21

A15

D66

D60

C43

C37 H83

A6

A12

C52B29 H74

H80

A19

A13

D64

D58

C41

C35

I-86

H81

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Documents

Victoria, BC July 27 – 31, 2019 · Presentation. International 2 3 Gap Junction Conference International Gap Junction Conference #IGJC2019 @int_gap #IGJC2019 WELCOME LETTER FROM