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ESSENTIALS THE of Quality Assurance & Quality Control OVER 450 PRODUCTS to Meet your QA/QC Needs DISPO POWDER DISPENSING TECHNOLOGY page 7 | A REVOLUTIONARY ANALYTICAL BALANCE FOR INCREASED PRODUCTIVITY page 8 | PROTEIN DETERMINATION FOR MEAT AND MEAT PRODUCTS USING THE KJELDATHERM AND VAPODEST UNITS page 20

VWR The Essentials of QA/QC Brochure, Lit. No. 92933

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Page 1: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

EssEntialsThe

of Quality Assurance & Quality Control

Over 450 PrOducts to Meet your QA/QC Needs

Dispo™ powDer Dispensing TeChnology page 7 | A revoluTionAry AnAlyTiCAl BAlAnCe for inCreAseD

proDuCTiviTy page 8 | proTein DeTerminATion for meAT AnD meAT proDuCTs using The KjelDATherm

AnD vApoDesT uniTs page 20

Page 2: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Contents

instrumental analysisElectrochemistry.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5

Environmental. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Mass.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-10

DisPo™ Powder Dispensing Technology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

A Revolutionary Analytical Balance for Increased Productivity.. . . . . . . . . . . . . . . . . . . . . .8

Microbiological testingLiving Organisms.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Media.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-12

Media Preparation.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13-14

Microscopy.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15-16

Incubators.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16-17

Wet analysisDigestion.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18-22

Protein Determination for Meat and Meat Products Using theKjeldatherm and VapoDest Units. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20-21

Distillation and Dilution.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21-23

Filtration.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24-25

Liquid Handling.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26-27

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Tight quality control is essential for

your production environment. VWR

has gathered a wide selection of

products in this issue to help you

achieve your quality assurance goals.

In addition, we have included technical

information that will help you get

the best use out of your equipment.

Page 3: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

abbe-3l Refractometer, thermo scientific• Accurate to ±0.0001 for refractive

indices of 1.3 to 1.71 and ±0.05% for 0–85% on the the total solids scale

• Easy three-step operation• Adjustment knob makes "micrometer

precise" adjustments when setting the dividing line

• Unit is designed for easy cleaning and servicing

Description Cat. No. EachAbbe Refractometer 55596-023 4386.82AccessoriesEyepiece 55596-158 163.20Lamp 55596-191 30.62Test Piece, Nom. Index 1.5125 55596-089 183.60

ORiOn® aQUafast® iV Colorimeter, thermo scientific• Allows custom methods using up to five data points

and will store up to ten custom methods in memory• Stores up to 189 pre-programmed methods• Simple interface provides displays for concentration,

absorbance, or percent transmittance• Auto-ID™ automatically matches the correct method,

wavelength, and reaction timer with the appropriate Auto-Test™ reagent

• A datalog of up to 100 points with time and date tags can be dowloaded via the RS-232 cable

• Waterproof to IP67 standards, allowing immersion in water up to a depth of 1m for one hour without any water incursion

DescriptionMeasurement Range, mg/L Cat. No. Price

AQUAfast IV Colorimeter — 14217-920 Ea./ 1053.00Auto-Test ReagentsChlorine (Free and Total) 0.40–6.0 14217-878 Pk. 30/ 36.00Iron 1 (Total and Soluble) 0.45–7.0 14217-870 Pk. 30/ 39.00Nitrate 1 (as N), Low Range 0.15–1.5 14217-844 Pk. 30/ 69.00Phosphate 1, High Range 5.0–80.0 14217-852 Pk. 30/ 38.00Sulfate 5.0–70.0 14232-937 Pk. 30/ 40.00Zinc 0.30–3.0 14217-876 Pk. 30/ 40.00

Portable Colorimeters, Hach®

• Designed for on-site testing with waterproof, dustproof, and chemical-resistant housings

• Can store up to 99 measurements with no delay in testing

• Features automatic wavelength selection

• Unit will store up to ten custom procedures with as many as 12 data points each

Refracto Portable Refractometers, MEttlER tOlEDO®

• Ideal for measurements in laboratory or production environments• Store up to 1100 measurement results and transmit to printer or PC via infrared

interface• Contain 11 pre-programmed units of measurement and the ability to enter user-

defined units• Temperature compensation coefficient is programmable for up to ten different

samples• Refracto 30PX uses an optical glass prism held by a stainless steel ring• Refracto 30GS uses a sapphire prism held by a gold-plated brass ring for extended

measuring range• Both models include carrying case, two sampling pipets, two sample flasks, cleaning

tissues, operating instructions, software, and batteries

Description Cat. No. EachRefracto 30PX 11276-898 1850.00Refracto 30GS 11276-900 2500.00

DescriptionNo. of Parameters Cat. No. Each

DR/850 Portable Colorimeter 50 97009-582 971.46DR/890 Portable Colorimeter 90 97009-588 1097.90

Reagents For Use WithMeasurement Range, mg/L

Cat. No. Pack of

Ammonia (as NH3-N), Low Range

DR/850 and DR/890 0.0–2.5 97009-556 50/ 90.10

Ammonia (as NH3-N), High Range

DR/850 and DR/890 0.0–50.0 97009-572 50/ 82.68

COD, Low Range DR/890 3–150 97009-532 25/ 38.24COD, Low Range DR/890 3–150 97009-592 150/ 208.85COD, High Range DR/850 and DR/890 20–1500 97009-538 25/ 38.24COD, High Range DR/850 and DR/890 20–1500 97009-590 150/ 208.85COD, High Range Plus DR/850 and DR/890 200–15,000 97009-594 150/ 208.85Nitrate (as N) DR/890 0.0–30.0 97009-576 50/ 69.00Nitrogen (Total) DR/890 0.0–25.0 97009-596 50/ 119.35Phosphorus (As Acid Hydrolyzable PO4)

DR/850 and DR/890 0.06–3.5 97009-568 50/ 56.00

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Instrumental analysIselectrochemistry

Page 4: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Quick-Brix™ Refractometers, MEttlER tOlEDO®

• Ideal for use on farms, vineyards, or juice presses• Portable, rugged, compact, splash-proof, and ideal for on-location

measurement• Features a built-in temperature compensation and single key

calibration

Description Accuracy, % Range, % Brix Cat. No. EachQuick-Brix 60 ±0.02 0–60 97005-214 430.00

VWR® spectrosil spectrophotometer Cells• Spectrosil quartz provides accurate readings over the entire UV range

to near infrared range• Fully heat-fused construction means there is no optical or physical

distortion• Transmission tolerance falls within 1.5% at 200nm• Choose flat PTFE lids for standard tests, stoppered cells for volatile

liquids, and screw caps for anaerobic conditions

External Dimensions, W x L x H, mm

Z Dimension, mm Lid Type

Pathlength, mm Volume Cat. No.

Pack of 2

Sub-Micro Cells with Black Sides12.5 x 12.5 x 45 8.5 Flat 10 50 µL 414004-049 292.4312.5 x 12.5 x 45 8.5 Flat 10 100 µL 414004-051 291.6412.5 x 12.5 x 45 15 Flat 10 50 µL 414004-050 292.4312.5 x 12.5 x 45 15 Flat 10 100 µL 414004-052 292.43Micro Cells12.5 x 12.5 x 45 — Flat 10 700 µL 414004-053 193.52Standard Rectangular12.5 x 3.5 x 45 — Flat 1 350 µL 414004-060 559.5212.5 x 7.5 x 45 — Flat 5 1.7 mL 414004-061 447.41

10 x 10 x 52 —Closed Screw Cap with Gasket

10 3.5 mL 414004-054 185.75

10 x 10 x 52 —Open Screw Cap with Septum

10 3.5 mL 414004-055 239.74

12.5 x 12.5 x 45 — Flat 10 3.5 mL 414004-062 554.9012.5 x 12.5 x 45 — Stopper 10 3.5 mL 414004-065 545.9212.5 x 42.5 x 45 — Two Stoppers 40 14 mL 414004-063 847.58

Plug-and-analyze Instruments, Perkinelmer®

Built to Last l Easy to Use l Even Easier to Own

spectrum two transportable Ft-iR spectrophotometer systems• Low maintenance systems are portable, yet feature benchtop sensitivity• Easily and safely turn the instrument off between runs• Application packages are available for pharmaceutical, food, and teaching environments

advanced UV/Vis series spectrophotometers• Lambda Bio+ is recommended for the following applications: molecular

biology, DNA/RNA, oligo, protein, and general teaching applications• Lambda XLS is a general-purpose spectrophotometer for teaching and

environmental laboratories• Lambda XLS+ provides enhanced resolution for quality control in food,

environmental, chemical, and R&D applications• Lambda 25 models are ideal for working in a regulated industry where large

sample quantites are processed every day

These small systems have been designed to fit into any pharmaceutical, food, or teaching laboratory. They are equipped with pre-programmed methods and plug-analyze setup, so that you can invest your time in data analysis, rather than data generation.

F cusnformation

F cusnformation

With over 12,000 units installed worldwide, these units have proven to deliver

consistent reliable results even under the toughest conditions.

Description Cat. No. EachLambda Bio+ with Memory Card 97024-682 6300.00Lambda Bio+ with Printer 97024-680 6400.00Lambda XLS with Printer 97024-664 5700.00Lambda XLS+ with Memory Card 97024-672 6000.00Lambda 25 Spectrophotometer Only 97024-422 10,000.00Lambda 25 with ES Software for 21CFR Part 11 Requirements 97024-430 11,500.00Lambda 25 with ES Software and PC for 21CFR Part 11 Requirements 97024-432 13,600.00Lambda 25 with PC 97024-424 11,800.00

Description Cat. No. EachSpectrum Two with KBr Window and Automated Validation 89187-904 15,100.00Spectrum Two with Cooled Detector, KBr Window, and Automated Validation for High Performance

89187-894 16,400.00

Spectrum Two with Automated Validation and Spectrum 10 Software for 21CFR Part 11 Requirements

89187-888 17,000.00

Universal Diamond ATR, Single Reflection 89187-912 6800.00

Instrumental analysIselectrochemistry

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Page 5: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Orion® star® a111 pH Benchtop Meter, thermo scientificCombine Simplicity with Accuracy• Ready indicator alerts let you know when readings are stable and AUTOREAD™ locks in the stable

reading• Up to three-point calibration• Automatically recognizes USA/NIST and DIN buffers• Non-volatile memory holds up to 50 data points• Included electrode arm and probe holder make it easier to place probes into samples• Meter kit includes Orion Triode™ 3-in-1 pH/ATC probe and solution kit with 60mL each of pH 4, 7,

and 10 buffers; cleaning solution; and storage solution• All-in-one buffer kit includes 475mL each of 4.01, 7.00, and 10.01 buffers; pH storage solution;

and pH electrode storage bottle• Three-year warranty

Description Cat. No. EachBenchtop Meter Kit 89206-280 723.00Benchtop Meter Only 89206-278 490.00AccessoriesElectrode Arm 89206-426 149.00Freestanding Base for Electrode Arm 89206-428 50.00

Orion Triode 3-in-1 pH/ATC Probe, Gel-Filled 10010-788 236.00Orion Triode 3-in-1 pH/ATC Probe, Refillable 10010-734 331.00

All-in-One pH Buffer Kit 14218-368 88.00Buffer, pH 4.01, 475 mL 34182-306 17.00Buffer, pH 7.00, 475 mL 34182-386 17.00Buffer, pH 10.01, 475 mL 34182-390 17.00pH Electrode Storage Solution, 475 mL 34108-041 50.00

F cusnformation

Get the information you need quickly and easily from the large, backlit graphic LCD display. The informative display shows pH/mV/RmV/ISE results and temperature along with electrode status, time, date, sample ID, user ID and calibration points. For advanced users, features such as stability and averaging options and flash expansion through the USB port provide additional options.

FoCus on your Core aCtIvItIes and maxImIze your ProduCtIvIty wIth vWrCATAlysT.

vWrCATAlysT offers a full portfolio of validation and calibration services to cover all of your preventative maintenance needs, as well as other services such as chemical inventory management, supply chain management, and logistical support. Contact your vwr sales representative or call 1.888.793.2300 for more information on vWrCATAlysT.

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Instrumental analysIselectrochemistry

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Page 6: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Professional and standard level Halogen Moisture analyzers, MEttlER tOlEDO®

• Easy-to-use drying process is up to twice as fast as infrared

• Automated sample drawer makes sample handling effortless

• Repeatability: ±0.02% for a 10g sample

HB43-s Compact analytical Halogen Moisture analyzer, MEttlER tOlEDO®

• Ideal for routine use in laboratory or production environments• Circular halogen lamp provides rapid and uniform sample heating

for highly reproducible results• Features over 100 ready-to-use methods that correspond to official

regulations• Easy chamber access simplifies cleaning and maintenance

Description Cat. No. EachCompact Halogen Moisture Analyzer 97003-990 3962.00

Mas-100Eco™ air sampler system, EMD Millipore• Specifically designed for monitoring microbial air quality in the food and beverage industries to

ensure a safe manufacturing environment• Uses standard 100mm petri dishes• Features a built-in calibration reminder that can be set between 1 and 12 months and a sampling

start delay of up to 60 minutes• Calibration is easy with DA-100™ anemometer• Will sample a full cubic meter of air in 10 minutes• Aspiration rate: 100L/min• UL listed, CE marked

Description Cat. No. EachAir Sampler System EM1.09227.0002 4890.81Anemometer EM4.86640.0001 5645.30

• Weighing resolution: 1mg at 200°C• CSA NRTL/C Certified

Standard Level Models• Offer a wide measuring range suitable for broader precision needs with excellent repeatability• Store up to ten reference methods• Provides results in a single line• Capacity: 61g

Professional Level Models• Feature integrated instrument check, three level password protection, and traceable sample

identification that qualify these units for use in regulated areas• Offers a multi-step drying program• AutoMet function automatically finds the optimum method for any given sample• Store up to 40 reference methods• Results can be reported as single line, statistics, or via a journal• Capacity: 81g

Description Cat. No. EachStandard Level ModelsMoisture Analyzer 11276-892 5675.00Moisture Analyzer with Printer 11276-894 6223.00Professional Level ModelsMoisture Analyzer 11276-888 7113.00Moisture Analyzer with Printer 11276-890 7678.00

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Instrumental analysIsenvironmental

Page 7: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

BioDot DisPo™ Handheld Dispensers• Designed for low throughput applications requiring multiple dispenses of solid compounds• Available in fine adjustment unit• Removable probes allow for easy cleaning• Complete set comes with the dispenser and probes in a foam-fitted carrying case

Description Cat. No. EachComplete Adjustable Mass Dispenser Kit 89171-918 2855.00Adjustable Mass Dispenser Probes500 µg–2 mg Probe 89171-920 255.001–5 mg Probe 89171-922 255.005–10 mg Probe 89171-924 255.0010–100 mg Probe 89171-926 255.00

disPo™ Powder dispensing technology

The ability to dispense dry powders and solids is a valuable tool for materials discovery, development, and optimization. The DisPo Powder Dispensing Technology provides an automated means to deliver a wide variety of powders over the mass range of 100μg to 20mg.

A key consideration for dispensing powders is the minimum amount of sample required. This is the amount of material that must be present in order to sample and dispense a given mass of powder. While this minimum sample amount is somewhat related to the target mass to be dispensed (in that it dictates the sample probe size, which in turn determines some minimal vessel geometry), the ideal scenario is to be able to sample from as small a starting mass as possible and to be as efficient in the use of this material as possible. Delivering low mass samples from these small starting masses is also a common consideration. A typical applica-tion would involve delivering 20-50 individual samples of 50-100µg from the same sample vessel.

Powders are dispensed via a volumetric delivery from a sample probe. This volume, combined with the bulk density of the powder being sampled, determines the mass that is delivered. There is an adjustable plunger within the sampling probe that allows for the setting of a fill height within the probe. This fill height and the diameter of the probe determine the sampling volume of powder. The sample probe is lowered into the powder, where it extracts the determined volume of powder. The probe is then moved out of the source location and moved over to the destination location with the plunger still being retracted. Once over the destination location, the plunger is actuated and the powder is delivered into the source location. This entire process is automated and programmable though the control software interface of the DisPo Powder Dispensing Systems.

Adjustable mass Dispenser Kit

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Instrumental analysIsmass

Page 8: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

a revolutionary analytical Balance for Increased Productivityian Ciesniewski, mettler-Toledo, inc.

Weighing is the most fundamental operation in the ana-lytical laboratory, and laboratories continually look for new developments and configurations to help increase productivity. The latest revolution comes in the form of the Excellence (XS) line of semimicro and analytical balances from Mettler-Toledo, Inc. With the new Smart-Grid™ suspended weigh pan, weighing is more accurate and precise than ever before (Figure 1).

The revolutionary SmartGrid weigh pan, suspended from the inside of the weighing chamber, is a more efficient placement than the traditional weigh pan placed at the bottom of the balance. The grid-style weighing promotes rapid and accurate analytical weighing by allowing air to freely pass through the grid, not affecting results. In addition, the suspended weigh pan leaves the space below completely sealed with no openings or gaps. If the sample drops to the bottom of the balance, the weighing result remains unaffected since it falls to the sealed platform. A simple wipe with a balance brush effectively cleans away any spillage.

To complement the grid-style weigh pan, special Ergo-Clip™ accessories can be used. ErgoClips accommodate special tare containers that are often used in the labora-tory, securing them unshakably to the weighing pan and ensuring safe handling and weighing. ErgoClips are offered in a weighing kit, which is available separately with a single-use aluminum pan and a set of weighing boats. This accessory set allows the user to tailor the balance to perfectly suit almost any application.

Enhancing the cleaning feature of the suspended weigh pan is a new draft shield design. The chemical- and acid-resistant draft shield can be completely dismantled and

placed in the dishwasher, along with the weighing pan and Ergoclips.

Another common problem in laboratories is space. To accommodate a shortage of space, the XS operating terminal can simply be removed and placed at the side or behind the balance. This allows considerable flexibility; the angles of the terminal can also be adjusted to suit lighting levels and ensure optimal readability.

The TouchScreen touch-sensitive screen, introduced on the AX/MX/UMX balances, brings further efficiency to the weighing operation. It offers fast operation and direct access to the menu. Three different display settings aid efficient, personalized operation.

The Excellence line is also equipped with a full suite of user software. Many common applications are standard, including clear-text dialogue for density determination of solids, liquids, or pasty substances. Other applications included are weighing, statistics, and formulation.

Overall, the Excellence line of analytical and semimicro balances establishes a high standard of performance for routine weighing operations in the laboratory. The revolutionary new technology in the SmartGrid weigh pan in the XS balance will improve the efficiency of all weighing tasks in the laboratory. The features that are most valuable to the user include the SmartGrid weigh pan, accessory clips for secure attachment of special tare containers to the weigh pan, a draft shield that can be completely dismantled to facilitate cleaning, an adjust-able terminal to accommodate space requirements, and a touch-sensitive screen for speed of operation.

figure 1 smartgrid

OVERALL, THE ExCELLEnCE LInE OF AnALyTICAL AnD SEMIMICRO BALAnCES ESTABLISHES A HIGH STAnDARD OF PERFORMAnCE FOR ROUTInE WEIGHInG OPERATIOnS In THE LABORATORy.

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Instrumental analysIsmass

Page 9: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Excellence Plus level, XP series analytical Balances, MEttlER tOlEDO®

• Use for secure and ergonomic weighing of small sample sizes• Multicolor touch screen displays 12 different weighing units and five applications, including statistics, formulation, and density• Fully-automatic internal calibration comes with history function• Operating software can be upgraded via the Internet• SmartGrid™ weighing pan is supported at the rear of the weighing chamber for more efficient weighing• When used with the optional weighing kit, the system can be precisely adapted to specific tasks• Pan size: 7.8 x 7.3 cm (3

1/16 x 2

7/8")• UL listed, CSA certified

Capacity, gFine Range Capacity, g

Fine Range Readability, mg

Linearity, mg

Readability, mg

Repeatability (Std. Dev.)

Stabilization Time, s Cat. No. Each

Balances with Fine Range Capability120 31 0.01 0.15 0.1 0.015–0.05 6 11278-320 8959.00220 81 0.01 0.15 0.1 0.015–0.06 6 11278-324 11,308.00520 101 0.1 0.5 1.0 0.1–0.6 4 11278-330 9085.00Balances with Standard Capability220 — — 0.2 0.1 0.05–0.07 4 11278-326 8959.00220 — — 0.1 0.01 0.015–0.03 6 11278-322 12,745.00520 — — 0.4 0.1 0.07–0.1 4 11278-328 10,478.00

Ms series analytical and Precision Balances, MEttlER tOlEDO®

• Feature fully-automated calibration technology using an internal weight and programmable smart keys for shortcut access to preferred applications

• Equipped with functions such as piece counting, percent weighing, and dynamic weighing• Balance housings are constructed of die-cast aluminum and weighing pans from chromium-nickel steel• The high-contrast display features large numbers and an intuitive menu• Pan size: 9 cm dia. (3

1/2")• UL listed, CSA certified

Capacity, g Linearity, mg Readability, mg Repeatability (Std. Dev.) Stabilization Time, s Cat. No. Each120 0.2 0.1 0.1 2 97035-620 3341.00220 0.2 0.1 0.1 2 97035-622 4052.00320 0.3 0.1 0.1 3 97035-624 4589.00

Excellence level, Xs series analytical Balances, MEttlER tOlEDO®

• Use for a variety of applications in pharmaceutical, chemical, food, cosmetic, research, and development applications• Feature backlit graphic display with touch-screen operation, fully automatic calibration, and a built-in RS-232 interface• SmartGrid™ weighing pan is supported at the rear of the weighing chamber for more efficient weighing• When used with the optional weighing kit, the system can be precisely adapted to specific tasks• Draft shield can be completely dismantled for easy cleaning• Pan size: 7.8 x 7.3 cm (3

1/16 x 2

7/8")• UL listed, CSA certified

Capacity, gFine Range Capacity, g

Fine Range Readability, mg

Linearity, mg

Readability, mg

Repeatability (Std. Dev.)

Stabilization Time, s Cat. No. Each

Balances with Fine Range Capability120 41 0.01 0.2 0.1 0.1 6 11276-876 5436.00220 81 0.1 1.0 1.0 0.7 4 11276-878 4686.00220 81 0.01 0.2 0.1 0.1 6 11276-882 6709.00Balances with Standard Capability61 — — 0.2 0.1 0.1 4 11276-872 3826.00120 — — 0.2 0.1 0.1 4 11276-874 4594.00220 — — 0.2 0.1 0.1 4 11276-880 5386.00

Ml series Portable analytical Balances, MEttlER tOlEDO®

• Ideal for routine weighing tasks• Programs include piece counting, percent weighing, and dynamic weighing• Backlit display allows for easy reading, even under extreme lighting conditions• Units are equipped with an internal MonoBloc weighing cell• Pan size: 9 cm dia. (3

1/2")• UL listed, CSA certified

Capacity, g Linearity, mg Readability, mg Repeatability (Std. Dev.) Cat. No. Each52 0.2 0.1 0.1 97035-662 2300.00120 0.2 0.1 0.1 97035-664 2736.00220 0.2 0.1 0.1 97035-666 3078.00

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Instrumental analysIsmass

Page 10: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

lyfoCults® Plus Quant and lyfoCults Plus Microorganisms, bioMerieux• Ready-to-use vials are glass-free, all-in-one devices that contain

stabilized, lyophilized, viable microorganisms and a built-in inoculation wand

• LyfoCults Plus Quant vials deliver less than 100 CFU/0.1mL and are ideal for quantitative growth promotion studies and other applications that validate the performance of culture media in industrial settings

• Meet USP recommendations

Description ATCC No. Cat. No. Pack of 10LyfoCults Plus MicroorganismsAspergillus niger 16404 95008-690 39.15Bacillus subtilis spizizenii 6633 95008-902 26.06Campylobacter jejuni 29428 95009-224 39.15Campylobacter jejuni 33291 95009-234 39.15Campylobacter jejuni 33560 95009-254 39.15Candida albicans 10231 95009-326 34.02Clostridium perfringens 13124 95009-876 34.02Enterococcus casseliflavus 700327 95010-480 26.06Escherichia coli 8739 95010-964 30.69Escherichia coli 25922 95010-822 22.50Listeria innocua 33090 95012-146 34.02Listeria ivanovii ivanovii 19119 95012-156 34.02Listeria monocytogenes, Serotype 4b

19115 95012-176 34.02

Pseudomonas aeruginosa 9027 95013-438 26.06Pseudomonas aeruginosa 27853 95013-428 22.50Saccharomyces cerevisiae 2601 95013-720 34.02Saccharomyces cerevisiae 9763 95013-700 34.02Staphylococcus aureus 6538 95014-276 30.69LyfoCults Plus Quant MicroorganismsClostridium sporogenes 19404 95009-972 273.62Staphylococcus epidermidis 12228 95014-514 273.62

sPORtROl® spore suspensions, naMsa®

• Pure suspensions of viable spores from specific organisms with known resistance to various sterilization processes

• Use to provide validation that bacterial spores have been completely destroyed

• Spores are suspended in water for injection and provided in 10mL pharmaceutical vials

• 106 spores/0.1mL• Meet USP and ISO 11138-1 standards

Application Organism Cat. No. EachEthylene Oxide or Dry Heat Sterilization

Bacillus atrophaeus 19872-022 145.41

Radiation Sterilization Bacillus pumilus 19872-026 156.04

Steam SterilizationGeobacillus stearothermophilus

19872-024 349.34

VWR® Microcentrifuges• Achieve optimum performance in a

compact unit• All operating parameters are set

and displayed digitally• Larger models (Micro 1814, 1816,

and 2416) feature brushless motors for faster acceleration and quieter, smoother operation

• Units are supplied with a fixed-angle microtube rotor that can be easily removed for cleaning or autoclaving

• Rotors accept 1.5 /2.0mL tubes directly and can accept smaller tubes with optional adapters

Model Rotor Space Max. Speed, rpm/g Cat. No. EachMicro 1207 12 Tubes 10,000 /7,200 37001-294 1032.44Micro 1814 18 Tubes 13,000 /14,000 37001-296 1836.77Micro 1816 18 Tubes 14,000 /16,000 37001-298 2087.81Micro 2416 24 Tubes 13,000 /16,000 37001-300 2340.73

VWR® symphony™ 4417/R Centrifuges• Compact, space saving design• Corrosion resistant, stainless steel rotor chamber• Quiet, maintenance-free induction drive• Imbalance detection with safety shut down• Microprocessor control with splash resistant

digital display• Accommodates tubes from 0.2mL to 100mL• Maximum speed: 13, 500 rpm/17,320 g

Description Cat. No. EachNon-Refrigerated Centrifuge 89171-142 3552.50Refrigerated Centrifuge 89171-144 7272.48

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Instrumental analysIsmass | living organisms

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Page 11: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

BD taxo™ P Discs for Differentiation of Pneumococci, BD Diagnostic systems• Recommended for use with pure cultures in

the presumptive identification of pneumococci• Made from high quality paper that has been

impregnated with ~5µg optochin• Optochin inhibits pneumococci, but not other

steptococci• Packaged 50 discs per cartridge• Size: 6mm (1/4")

Description Cat. No. Pack of 10P Discs for Pneumococci 90002-338 83.33

Durham tubes, bioMerieux• Use to detect coliform organisms in foods, water, wasterwater and other material of

sanitary importance• Presence of organisms is indicated by the presence of gas in the tube• 10mL broth in 16 x 125mL tubes

Description Cat. No. Pack of 100Brilliant Green Bile Broth 29447-452 214.24Lauryl Sulfate Broth 29447-490 295.80Lauryl Sulfate Broth, 2X 29447-492 350.20

BD BBl™ sterile Pack Plated Media, BD Diagnostic systems• Use to monitor critical environments; use isolator plates specifically to monitor

isolators• Two layers of Tyvek®/polyethylene wrapping create a bacterial barrier, while

allowing optimal moisture transfer• A sterile third inner bag is included for transport from the clean area to the testing

laboratory• Gamma-irradiated for sterility assurance and to eliminate the time and expense of

pre-incubation• Choose BD RODAC™ plates for surface and active air sampling• Choose settling plates for active and passive air sampling

DescriptionPlate Dia., mm Cat. No. Price

BD RODAC PlatesBD Trypticase™ Soy Agar with Lecithin and Polysorbate 80

100 90001-274 Pk. 10/ 50.60

BD Trypticase Soy Agar with Lecithin and Polysorbate 80

100 90001-614 Cs. 100/ 480.51

D/E Neutralizing Agar 100 90006-582 Cs. 100/ 528.56Sabouraud Dextrose Agar 100 90006-192 Pk. 10/ 51.54Sabouraud Dextrose Agar with Lecithin and Polysorbate 80

100 90005-314 Pk. 10/ 31.40

Standard Methods Agar with Lecithin and Polysorbate 80

150 90006-550 Pk. 20/ 117.50

BD RODAC Isolator PlatesBD Trypticase Soy Agar with Lecithin and Polysorbate 80

100 90005-296 Pk. 10/ 30.11

BD Trypticase Soy Agar with Lecithin and Polysorbate 80

100 90004-226 Cs. 100/ 287.92

BD Trypticase Soy Agar with Lecithin, Polysorbate 80, and Penicillinase

100 90006-190 Pk. 10/ 57.65

CDC Anaerobic Blood Agar 100 90007-732 Pk. 10/ 37.95D/E Neutralizing Agar 100 90007-906 Pk. 10/ 32.72D/E Neutralizing Agar 100 90007-908 Cs. 100/ 314.09D/E Neutralizing Agar with Penicillinase 100 90004-160 Pk. 10/ 39.94Rose Bengal Agar with Penicillinase 100 90004-156 Pk. 10/ 30.09Sabouraud Dextrose Agar with Lecithin and Polysorbate 80

100 90001-268 Pk. 10/ 55.43

Settling PlatesBD Trypticase Soy Agar 100 90001-270 Pk. 5/ 49.46BD Trypticase Soy Agar 100 90001-610 Pk. 10/ 480.51BD Trypticase Soy Agar 150 90001-272 Cs. 100/ 24.61BD Trypticase Soy Agar, Triple Wrapped 150 90001-612 Cs. 45/ 303.11BD Trypticase Soy Agar with Lecithin, Polysorbate 80, and Penicillinase

100 90004-197 Pk. 100/ 301.01

BD Trypticase Soy Agar with Penicillinase 150 90007-750 Pk. 45/ 235.57BD Trypticase Soy Agar, Heavy Fill (25mL) 100 90007-744 Pk. 10/ 53.70BD Trypticase Soy Agar, Heavy Fill (25mL) 100 90007-781 Pk. 100/ 274.84BD Trypticase Soy Broth with Agar 100 90001-557 Pk. 5/ 50.21m-HPC Agar 100 90004-236 Cs. 100/ 353.38Isolator Settling PlatesBD Trypticase Soy Agar 100 90007-918 Cs. 100/ 30.11BD Trypticase Soy Agar 100 90007-920 Pk. 10/ 295.96BD Trypticase Soy Agar, Heavy Fill (25mL) 100 90004-238 Pk. 5/ 301.01BD Trypticase Soy Agar 150 90004-178 Pk. 10/ 26.99R2A Agar 100 90004-234 Cs. 100/ 287.92Rose Bengal Agar with Penicillinase 100 90004-158 Cs. 100/ 31.40

do you need a CustomIzed medIa PreParatIon solutIon?

for a number of years, vWrCATAlysT team members have been performing various types of media prep and sample testing. Today, we have the people, processes, and technology to offer a customized solution for virtually any media prep or sampling problem. Call 1.888.793.2300 or visit www.vwrsp.com/vwrcatalyst for more information.

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mICroBIologICal testIngmedia

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Page 12: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

media solutions for usP ComplianceMicrobiological Examination of Non-sterile Products

BD Dehydrated and Prepared Media for non-sterile Product testing• Formulation, QC release testing, labeling, and Certificates of Analysis meet harmonized USP/EP/JP requirements• Quality System compliant with ISO 9001 and 13485 - Customer audits welcome• Available in various media configurations and packaging sizes• Over 170 years of combined Difco™ and BBL™ microbiology experience

Microbiology - it’s what we do.

BD Biosciences

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mICroBIologICal testIngmedia

Page 13: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

VWR® Microincinerator• Quickly and safely sterilize metal loops and needles without using

an open flame• No need to wait for the metal to glow, sterilization is complete

within 7 seconds• Infrared heat provides sterilization at 815°C (1500°F)• Heating element is protected by a stainless steel cowl• Cast aluminum tripod base features suction feet for stabilization• Unit can be adjusted to five different positions in 8° increments

Electrical Cat. No. Each120V, 60Hz, 1.1A 80094-500 451.41220V, 50Hz, 0.6A 80094-502 423.53

VWR® Polypropylene Cell spreaders• Disposable spreaders are designed to spread bacterial cell cultures

evenly• L-shape allows application to the entire petri dish or plate surface• Sterile

Packaging Cat. No. Pack of10/Bag 89042-018 500/ 95.671/Bag 89042-021 100/ 37.40

VWR® Disposable inoculating loops and needles• High-impact polystyrene creates a smooth and

flexible surface to facilitate uniform and smooth streaking without damaging the gel surface

• Color coded for easy size identification• Packaged in tamperproof, zip-seal resealable bags• Sterile• Total length: 22.7cm (8

7/8")

Size Color Packaging Cat. No. Case ofLoops1 µL Blue 1/Bag 89126-870 250/ 58.321 µL Blue 10/Bag 12000-808 1000/ 171.961 µL Blue 25/Bag 12000-806 1000/ 119.4510 µL Yellow 1/Bag 89126-872 250/ 558.3210 µL Yellow 10/Bag 12000-812 1000/ 171.9610 µL Yellow 25/Bag 12000-810 1000/ 119.45Needles— White 1/Bag 89126-868 250/ 558.86— White 10/Bag 12000-816 1000/ 173.61— White 25/Bag 12000-814 1000/ 119.45

VWR® Reusable inoculating loops and needles• Constructed of high-quality nichrome wire that cools

rapidly after sterilization• Optional lightweight aluminum handles are insulated

for hand protection• Loops are calibrated and supplied with certificates

based on ASM/APHA Evans blue dye test protocols

Description Size Cat. No. PriceLoopsWithout Handles 2.3 mm I.D. 80094-476 Pk. 25/ 77.58Without Handles 3 mm I.D. 80094-478 Pk. 25/ 77.58Without Handles 3.6 mm I.D. 80094-480 Pk. 25/ 78.35Calibrated without Handle 1 µL 80094-496 Pk. 5/ 200.27Calibrated without Handle 10 µL 80094-494 Pk. 5/ 200.27Streaker with Handles 2.5 mm I.D 80094-490 Pk. 5/ 58.07Streaker with Handles 3.5 mm I.D. 80094-488 Pk. 5/ 58.07Streaker with Handles 5.5 mm I.D. 80094-486 Pk. 5/ 58.07Calibrated Streaker with Handle 1 µL 80094-510 Pk. 5/ 233.42Calibrated Streaker with Handle 10 µL 80094-492 Pk. 5/ 205.54NeedlesWith No Handle — 80094-508 Pk. 12/ 13.89Angled with 15.2 cm (6") Handle — 80094-507 Ea./ 24.24Straight with 15.2 cm (6") Handle — 80094-498 Ea./ 22.99Straight with 20.3 cm (8") Handle — 80094-504 Pk. 5/ 56.75

VWR® inoculating turntables• Hand-operated turntables product almost concentric

circles of bacterial colonies evenly distributed across petri dishes

• Constructed of cast iron and coated with baked acrylic enamel

• Small table accommodates 100mm petri dishes and features a tripod base to bring work to eye level

• Large table accommodates either 100mm or 150mm petri dishes and is designed to sit close to the work surface

Description Dia. x H, cm (in.) Cat. No. EachSmall Turntable 11.4 x 7.6 (4

1/2 x 3) 50809-022 151.45Large Turntable 15.2 x 3.5 (6 x 1

3/8) 50809-020 188.40

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mICroBIologICal testIngmedia preparation

Page 14: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

VWR® Culture tubes with Dual-Position Caps• Polypropylene tubes are autoclavable to 122°C (252°F) and freezable to –90°C (–194°F)• Polystyrene tubes are also available• Polyethylene dual-position caps allow sample aspiration• Caps for nonsterile tubes are packaged separately

O.D. x L, mm Volume, mL Sterility Packaging Cat. No. Case ofPolypropylene Culture Tubes12 x 75 5 Sterile 25/Bag 60818-576 500/ 132.3112 x 75 5 Sterile 125/Bag 60818-500 1000/ 223.4412 x 75 5 Nonsterile Bulk 20170-579 1000/ 182.4917 x 100 14 Sterile 25/Bag 60818-725 500/ 186.4017 x 100 14 Sterile 125/Bag 60818-689 1000/ 304.7517 x 100 14 Nonsterile Bulk 60818-664 1000/ 180.12Polystyrene Culture Tubes12 x 75 5 Sterile 25/Bag 60818-565 500/ 132.9712 x 75 5 Sterile 125/Bag 60818-496 1000/ 233.5212 x 75 5 Nonsterile Bulk 60818-419 1000/ 193.2517 x 100 14 Sterile 25/Bag 60818-703 500/ 170.8517 x 100 14 Sterile 125/Bag 60818-667 1000/ 288.6217 x 100 14 Nonsterile Bulk 60818-661 1000/ 193.70

VWR® Disposable Glass Culture tubes• Type I, Class B borosilicate glass is chemically inert

and provides a high heat tolerance• Feature well-rounded bottoms and fire-polished

tops• Dimensional specifications are rigidly controlled for

reliable, uniform results• Exceed USP Type I specifications

O.D. x L, mmOverflow Volume. mL Cat. No. Case of

6 x 50* 0.75 47729-566 2000/ 195.6110 x 75 3 47729-568 1000/ 67.4612 x 75 5 47729-570 1000/ 75.6913 x 100 9 47729-572 1000/ 91.9415 x 85 10 47729-574 1000/ 145.4016 x 100 14 47729-576 1000/ 125.8716 x 125 18 47729-578 1000/ 143.5616 x 150 22 47729-580 1000/ 151.7118 x 150 28 47729-583 500/ 112.5920 x 150 34 47729-584 500/ 182.7425 x 150 60 47729-586 500/ 203.77*Also known as a Durham Tube

VWR® anaerobic Petri Dishes• Virgin polystyrene dishes are nonvented for

use with oxygen sensitive microorganisms and other anaerobic applications

• Provide distortion-free visibility• I-Plate dishes have bottoms divided into

two sections• Pill pocket dishes feature a pocket for

CO2 tablets on the inside wall of the bottom dish

• Semi-stackable dishes feature rings on

VWR® Petri Dish sets• Feature reinforced beaded edges to resist mechanical breakage• Glass dishes will remain clear after repeated wet or dry sterilization

cycles• Covers are inscribed with white and bottoms are inscribed with blue

for easy sorting• Meet Federal Specification NNN-D-1478• Packaged as boxes of 12 covers and boxes of 12 bottom dishes

Dia. x H, mm Cat. No. Case of 60 x 15 89000-300 72/ 477.03100 x 10 89000-302 72/ 446.51100 x 15 89000-304 72/ 451.01100 x 20 89000-306 72/ 377.51150 x 20 89000-308 24/ 348.62

the cover and the bottom to help with stacking, but can still be used for automatic handling• Slippable dishes can slide freely on top of each other to facilitate automated handling• Packaged with 20 dishes and covers stacked in easy-open, linear-tear bags• Sterile

Description Dia. x H, mm Cat. No. Case of 500Gamma Radiation-SterilizedI-Plate 100 x 15 25384-320 294.87Pill Pocket 100 x 15 25384-318 276.23Semi-Stackable 58 x 13 25384-332 159.98Semi-Stackable 60 x 15 25384-328 164.49EO Gas SterilizedFully-Stackable 60 x 15 25384-164 145.32Semi-Stackable 60 x 15 25384-062 145.32Slippable 60 x 15 25384-166 145.32

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mICroBIologICal testIngmedia preparation

Page 15: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

VWR VistaVision™ Planachromatic Compound Microscope with trinocular Head• Features exceptional contrast and resolution, brilliant uniform illumination, and highly durable mechanical

elements• Siedentopf design viewing head with single diopter adjustment• Kohler illumination provides uniform intensity and 1.25 numerical aperture (N.A.) Abbe condenser with iris

diaphragm allows precise centering of the light path• Large frame provides a stable, vibration-free base• Coxial, mechanical stage features precise 2.0µm graduations• Planachromatic objectives minimize spherical and chromatic aberrations

Description Cat. No. EachBrightfield Trinocular Compound Microscope 82026-624 2289.99

HaRlECO® Microbiology and General Use stains, EMD Millipore• Manufactured with materials certified by the Biological Stain Commission• Gram staining set (15204-196) contains one 500mL poly bottle each of crystal violet, decolorizer, iodine, and safranin stain

Description Size Packaging Cat. No. EachCrystal Violet 4 x 500 mL (16.9 oz.) Poly Bottle 15204-189 70.84Crystal Violet 3.8 L (1 gal.) Poly Bottle 15204-288 125.14Decolorizer 4 x 500 mL (16.9 oz.) Poly Bottle 15204-195 64.87Decolorizer 3.8 L (1 gal.) Poly Bottle 15204-282 113.33Iodine 4 x 500 mL (16.9 oz.) Glass Bottle 15204-193 71.82Iodine 3.8 L (1 gal.) Poly Bottle 15204-284 125.14Loeffler’s Methylene Blue 1 L (33.8 oz.) Poly Bottle 15204-202 47.41Lugol’s Iodine 500 mL (16.9 oz.) Glass Bottle 15204-148 36.31Safranin 4 x 500 mL (16.9 oz.) Poly Bottle 15204-191 68.62Safranin 3.8 L (1 gal.) Poly Bottle 15204-286 110.97Sulfosalicylic Acid, 3% 1 L (33.8 oz.) Glass Bottle 34172-026 37.07Sulfosalicylic Acid, 20% 1 L (33.8 oz.) Glass Bottle 34172-070 80.39Gram Staining Set — — 15204-196 72.02

VWR VistaVision™ stereo Zoom Binocular Microscope• Ideal for industrial inspection, electronics assembly or bonding operations, and pharmaceutical inspection• Greenough optics system delivers non-distorted 3D images throughout the zoom range of 0.65X to 4.5X

(8:1 ratio)• Total magnification can be up to 180X, depending on the optional eyepiece and auxillary lens selected• Paired 10X Widefield eyepieces feature a working distance of 100mm and dual diopter adjustment• Illuminated stand includes variable controls for both transmitted and reflected light

Description Cat. No. EachHalogen/Fluorescent Illuminated Binocular Stereo Microscope 82026-644 1652.38

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mICroBIologICal testIngmicroscopy

Page 16: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

BD BBl™ and BD Difco™ acid-Fast Bacilli (aFB) stain Kits, BD Diagnostics• Bottles feature a hinged spout that allows for regulated flow• TB fluorescent stain kits enable the observation of smears at a lower magnification• Smears may be restained with an AFB stain kit to confirm the acid-fastness of fluorescing organisms

KB series Refrigerated Microbiology incubators, BinDER• DCT™ cooling system and APT.Line™ technology

create ideal conditions for highly accurate and constant temperature environments

• Inner chamber remains condensation-free during cooling

• Microprocessor controller allows for easy programming of variable temperature cycles

BF series Microbiology incubators, BinDER• Feature APT.Line™ preheating chamber for temperature precision and adjustable

fan speed• Ventiliation is adjustable through the rear exhaust duct with ventilation flap and

the front ventilation slide• Microprocessor control provides an integrated timer and numerous time/

temperature functions• RS-422 interface is complaint with GLP/GMP and FDA guideline CFR 21• Supplied with two stainless steel shelves• Temperature range: 5° above ambient to 100°C (212°F)• Temperature accuracy: ±0.2°C at 37°C (98.6°F)

DescriptionVolume, L (cu. ft.)

Exterior Dimensions, W x D x H, cm (in.) Electrical Cat. No. Each

BF 53 Model 53 (1.89)63.4 x 57.5 x 61.7 (25 x 22

5/8 x 24 5/16)

115V, 50/60Hz, 400W

80079-098 2484.94

BF 53 Model 53 (1.89)63.4 x 57.5 x 61.7 (25 x 22

5/8 x 24 5/16)

230V, 50/60Hz, 400W

80079-100 2484.94

BF 115 Model 113 (4)83.4 x 64.5 x 70.2 (32

7/8 x 25 7/16 x 27

5/8)115V, 50/60Hz, 400W

80079-094 3243.98

BF 115 Model 113 (4)83.4 x 64.5 x 70.2 (32

7/8 x 25 7/16 x 27

5/8)230V, 50/60Hz, 400W

80079-096 3243.98

BF 240 Model 240 (8.50)103.4 x 74.5 x 82.2 (40

3/4 x 29 3/8 x 32

3/8)115V, 50/60Hz, 680W

80079-090 4805.22

BF 240 Model 240 (8.50)103.4 x 74.5 x 82.2 (40

3/4 x 29 3/8 x 32

3/8)230V, 50/60Hz, 680W

80079-092 4805.22

BF 400 Model 400 (14)123.4 x 76.5 x 103 (48

5/8 x 30 1/8 x 40

9/16)115V, 50/60Hz, 850W

80079-086 6014.06

BF 400 Model 400 (14)123.4 x 76.5 x 103 (48

5/8 x 30 1/8 x 40

9/16)230V, 50/60Hz, 850W

80079-088 6014.06

BF 720 Model 720 (25.67)123.4 x 86.5 x 153 (48

5/8 x 34 1/16 x 60

1/4)115V, 50/60Hz, 1250W

80079-082 7876.51

BF 720 Model 720 (25.67)123.4 x 86.5 x 153 (48

5/8 x 34 1/16 x 60

1/4)230V, 50/60Hz, 1250W

80079-084 7876.51

These forced convection incubators are the highest class

for all gentle incubation tasks. They are ideal for large

batch requirements that require short temperature

recovery times.

Description For Use WithSize, mL (oz.) Cat. No. Each

Stain KitsTB Fluorescent Stain Kit M — 250 (8.4) 90008-882 76.04TB Fluorescent Stain Kit T — 250 (8.4) 90008-886 79.16TB Stain Kit K — 250 (8.4) 90008-888 82.97TB Stain Kit ZN — 250 (8.4) 90008-884 67.64Individual StainsAcridine Orange — 250 (8.4) 90000-706 91.47TB Auramine M TB Fluorescent Stain Kit M 250 (8.4) 90000-690 96.77

Description For Use WithSize, mL (oz.) Cat. No. Each

TB Auramine-Rhodamine T TB Fluorescent Stain Kit T 250 (8.4) 90000-692 126.04TB Brilliant Green K TB Stain Kit K 250 (8.4) 90008-890 61.33TB Carbolfuchsin KF TB Stain Kit K 4 x 250 (8.4) 90000-698 168.44TB Carbolfuchsin ZN TB Stain Kit ZN 250 (8.4) 90000-684 154.06TB Decolorizer TB Stain Kit ZN 4 x 250 (8.4) 90000-696 42.00TB Decolorizer TM TB Fluorescent Stain Kit T 250 (8.4) 90000-686 37.80TB Methylene Blue TB Stain Kit ZN 4 x 250 (8.4) 90000-694 65.01TB Potassium Permanganate TB Fluorescent Stain Kit T 250 (8.4) 90000-688 60.17

F cusnformation

F cusnformation

With its comprehensive program functions, these forced convection refrigerated incubators offer a wide range of capabilities that will provide reproducible results

• RS-422 interface is complaint with GLP/GMP and FDA guideline CFR 21

• Supplied with two stainless steel shelves• Temperature range: –10 to 100°C (14 to 212°F)• Temperature accuracy: ±0.1°C at 5°C (9°F);

±0.2°C at 40°C (72°F)

DescriptionVolume, L (cu. ft.)

Exterior Dimensions, W x D x H, cm (in.) Electrical Cat. No. Each

Model KB 23* 20 (0.67)43.3 x 51.6 x 61.8 (17 x 20

5/16 x 24 5/16)

230V, 50/60Hz, 340W

89038-318 4582.33

Model KB 53 53 (1.89)63.4 x 57.6 x 83.7 (25 x 22

11/16 x 33)230V, 50/60Hz, 460W

89182-834 4939.00

Model KB 115 113 (4)83.4 x 64.6 x 102.2 (32

7/8 x 25 7/16 x 40

1/4)115V, 50/60Hz, 460W

89038-312 5921.69

Model KB 115 113 (4)83.4 x 64.6 x 102.2 (32

7/8 x 25 7/16 x 40

1/4)230V, 50/60Hz, 460W

89038-314 5921.69

Model KB 240 240 (8.50)103.4 x 74.6 x 114.2 (40

3/4 x 29 3/8 x 45)

230V, 50/60Hz, 930W

89038-310 8493.98

Model KB 720 700 (24.67)123.4 x 86.7 x 181.6 (48

5/8x 34 1/8 x 71

1/2)208/240V, 60Hz, 1250W

89038-798 12925.70

Model KB 720 700 (24.67)123.4 x 86.7 x 181.6 (48

5/8x 34 1/8 x 71

1/2)230V, 50/60Hz, 1350W

89038-304 12925.70

*Temperature accuracy is ±0.2°C at 5°C (9°F)

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mICroBIologICal testIngmicroscopy | incubators

Page 17: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

i series Biological incubator shakers, new Brunswick• Benchtop model holds flasks up to 2.8L and features a clear lid for easy viewing• Space-saving stackable model holds flasks up to 2.8L, can be stacked up to three units high, and features a slide

out platform for easy access to samples• The large capacity console, or floor model can hold flasks up to 6L in size and features a large viewing window to

easily check cultures• All shakers are sold without the platform

Description Temperature Range, °C (°F) RPM Range Cat. No. EachBiological Shaker Model I24Incubated Shaker with 3/4" Orbit 7° above ambient to 60°C (140°) 50 - 500 89173-942 7475.00Incubated/Refrigerated Shaker with 3/4" Orbit 15° below ambient to 60°C (140°) 50 - 500 89173-944 8754.00Incubated Shaker with 1" Orbit 7° above ambient to 60°C (140°) 50 - 500 89174-464 7475.00Incubated/Refrigerated Shaker with 1" Orbit 15° below ambient to 60°C (140°) 50 - 500 89174-466 8754.00Biological Shaker Model I26Stackable/Floor Incubated Shaker 5° above ambient to 60°C (140°) 25 - 400 89173-938 12,660.00Stackable/Floor Incubated/Refrigerated Shaker 15° below ambient to 60°C (140°) 25 - 400 89173-940 14,460.00Biological Shaker Model I2500Console Incubated Shaker 5° above ambient to 60°C (140°) 25 - 500 89173-946 12,576.00Console Incubated/Refrigerated Shaker 15° below ambient to 60°C (140°) 25 - 500 89173-948 14,260.00Console Incubated/Refrigerated Shaker with Programmable Timer

15° below ambient to 60°C (140°) 25 - 500 89173-950 15,225.00

VWR® symphony™ low-temperature/B.O.D. incubators• Forced air mechanical convection offers

excellent temperature uniformity for a wide range of applications

• Storage function allows samples to be kept at a specific temperature for the time period indicated by the user

• Feature a 3.3cm (1

5/16") stainless steel ventilation cap, one solid door, one glass door, and sensor error detection

• Units are insulated with glass wool and foamed silicone rubber door gaskets

• Temperature range: 0 to 60°C (32 to 140°F)• UL listed, CE marked

Volume, L (cu. ft.)Exterior Dimensions, W x D x H, cm (in.) Cat. No. Each

150 (5.25)64 x 83.3 x 131.3 (25

1/4 x 32 13/16 x 51

11/16)414004-626 3630.12

255 (9)64 x 83.3 x 170.2 (25

1/4 x 32 13/16 x 67)

414004-628 4317.28

425 (15)84.1 x 93.2 x 175.3 (33

1/8 x 36 11/16 x 69)

414004-630 5122.86

700 (24.67)93.5 x 102.6 x 199.9 (36

13/16 x 40 3/8 x 78

11/16)414004-632 7506.32

VWR® symphony™ Gravity Convection incubators• Provide a stable environment while

minimizing the potential of samples drying out

• Digital advanced adaptive microprocessor control system provides superior temperature accuracy

• Feature a 3.3cm (1

5/16" ) stainless steel ventilation cap, one solid door, one glass door, and sensor error detection

• Units are insulated with glass wool and foamed silicone rubber door gaskets

• Temperature range: 25 to 70°C (77 to 158°F)• UL listed, CE marked

Volume, L (cu. ft.)Exterior Dimensions, W x D x H, cm (in.) Cat. No. Each

32 (1.11)48 x 45.7 x 62.5 (18

7/8 x 18 x 24 5/8)

414004-610 1492.76

50 (1.75)54.1 x 51.8 x 70.1 (21

5/16 x 20 3/8 x 27

5/8)414004-612 1778.58

105 (3.71)64.5 x 57.7 x 84.1 (25

3/8 x 22 3/4 x 33

1/8)414004-614 2162.50

155 (5.40)70.9 x 64 x 90.7 (27

7/8 x 25 3/16 x 35

3/4)414004-616 2329.26

Known throughout the world for dependable operation and innovative design, new Brunswick offers the widest selection of shakers in the industry. The I Series biological shakers come in 3 sizes: benchtop, space-saving stackable models, or console, each offered with incubation only or incubation and refrigeration. All feature new Brunswick’s triple-eccentric counterbalanced shaking mechanism for uniform motion and years of quiet, dependable operation. Microprocessor controls with digital display enables easy operation and accurate setpoint regulation.

F cusnformation

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mICroBIologICal testIngincubators

Page 18: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Pulverisette® Planetary Mills, Fritsch• Ideal for comminution of solid matter in suspension

down to colloidal fineness, crushing dry laboratory samples, and homogenizing emulsions and pastes

• Grinding bowls and balls rotate around their own axis on a counter-rotating supporting disc

• Parameters can be controlled with a programmable electronic system and membrane keyboard

• Features RS-232 interface for data transfer of parameters

• Grinding bowls and balls are available in eight different materials and must be ordered separately;

Pulverisette® Vibratory Micro Mill, Fritsch• Ideal for fine milling of dry lab samples or

solids in suspension and for homogenizing emulsions and pastes

• Achieves size reduction through the combination of impact and friction

• Modular system permits simple conversion to dry- or wet sieving

• Spill-resistant membrane keyboard is ergonomically mounted

Pulverisette® Cutting Mills, Fritsch• Designed for grinding soft to medium-hard dry

materials, including fibrous materials and plastics• Laboratory mill comes with 3.5L collecting vessel• Universal mills use computer-optimized cutting

geometry to provide user-defined results• Cutting tool set and sieve inserts are not included;

contact your VWR Sales Representative for more information

• Manufacturer’s limited two-year warranty• Universal mills are CE marked

Description Electrical Cat. No. EachPulverisette 15 Laboratory Cutting Mill 100-120V, 50-60Hz 89033-414 9012.00Pulverisette 19 Universal Cutting Mill 100-120V, 50-60Hz 89033-418 16,315.00Pulverisette 19 Universal Cutting Mill 230V, 50-60Hz 97040-056 15,235.00Pulverisette 19 Universal Cutting Mill 400V/3, 50-60Hz 97040-054 15,235.00

Pulverisette® 14 Variable speed Rotor Mill, Fritsch• Designed for fine grinding of soft

and medium-hard samples, as well as temperature-sensitive samples

• High rotor speed enables fast and effective grinding by reducing thermal stress on the material

• Supplied with collecting vessel and lid• Voltage must be specified when

ordering• Manufacturer’s limited two-year

warranty• CE marked

Description Cat. No. EachPulverisette 14 Variable Speed Rotor Mill 89033-412 10,750.00

Pulverisette® 16 Cross Beater Mill, Fritsch• Ideal for grinding medium-hard and

brittle materials with a Mohs hardness up to 6

• Sample material is ground between a cross beater, a cast iron grinding insert, and a bottom sieve until it reaches the required fineness

• Supplied with a 5L collecting vessel and filter hose

• Bottom sieve must be ordered separately; contact your VWR Sales Representative for more information

• Manufacturer’s limited two-year warranty

• CE marked

Description Electrical Cat. No. EachPulverisette 16 Cross Beater Mill 110V, 60Hz 89033-416 10,300.00

contact your VWR Sales Representative for more information• Voltage must be specified at time of order• Manufacturer’s limited two-year warranty• CE marked

DescriptionNo. of Grinding Bowl Fasteners Cat. No. Each

Pulverisette 5 Mill 2 89033-410 23,231.00Pulverisette 5 Mill 4 97039-974 26,530.00Pulverisette 6 Mill 1 89033-406 10,990.00Pulverisette 7 Micro Mill 2 89033-408 11,690.00Pulverisette 7 Premium Micro Mill 2 97039-980 15,816.00Pulverisette 23 Mini Mill 1 89033-420 5666.00

• Grinding head is included• Grinding mortars and balls are available in eight different materials and must be

ordered separately; contact your VWR Sales Representative for more information• Manufacturer’s limited two-year warranty• CE marked

DescriptionNo. of Grinding Heads Cat. No. Each

Pulverisette 0 Micro Mill 1 89033-402 3936.00

Pulverisette® 2 Mortar Grinder, Fritsch• Ideal for fine milling of dry lab

samples or solids in suspension and for homogenizing emulsions and pastes

• Mortar is rotated by a gear motor that drives the mounted, free-rotating pestle through friction

• Specific pestle settings are provided for pre-crushing the samples and can be set using the membrane keyboard

• Grinding sets are available in eight different materials and must be ordered separately; contact your VWR Sales Representative for more information

• Voltage must be specified at time of order

• Manufacturer’s limited two-year warranty

• CE marked

Description Cat. No. EachPulverisette 2 Mortar Grinder 89033-404 8110.00

wet analysIsDigestion

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wet analysIsDigestion

Page 19: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

seward stomacher® lab Blenders, seward• Offer fast, reliable blending and homogenizing of laboratory samples• Paddles apply pressure to a sample in a sterile, disposable bag so that samples can

be collected, transported, processed, and disposed of without excessive handling• All models feature microprocessor controls, safety interlock on the stainless steel

front door, and stainless steel beater paddles• Timers can be set for 30, 60, or 120 seconds or for a continuous run• Model 80 Biomaster easily fits inside a safety cabinet• Model 400C features one-touch programmable time and speed settings, adjustable

timer beyond the three speed settings, and auto-run• The 3500 model series are designed for large blending volumes• Model 3500W also features a large blending area for bulky water filters• Model 3500T also features temperature control between 20 and 50°C (68 and 122°F)

Model Electrical Cat. No. Each80 Biomaster 110V, 60Hz, 75W 58976-902 5757.82400C 110V, 60Hz, 170W 58976-916 5881.27400C 220V, 60Hz, 170W 58976-960 6266.203500 110V, 60Hz, 90W 58976-941 13,372.293500W 110V, 60Hz, 90W 58976-950 13,924.873500T 110V, 60Hz, 420W 58976-952 22,876.57

VWR® sample Bags for the seward stomacher® Blender• Made of highly transparent polypropylene with wide-sealed bottoms

to prevent leaks• Standard bags feature a tear-off sterile barrier top to ensure sterility

up to the time of use• Closure bags permit collection, transportation, processing, and storage

of samples within the same bag• Bags with 4mil thickness are for analysis of firmer samples• FILTRA-BAG® bags are a mix of polyethylene and nylon with a dividing

filter membrane for optimal flow between compartments during blending

• SECURE-T® bags are made from heat-extruded polyethylene tubing for internal sterility and to eliminate the side seals

smasher® Blender, aes Chemunex• Twenty times quieter than other bag blenders• Crushes the sample in just 15 seconds and then

automatically adjusts the speed to ensure a perfect homogenization of the broth

• Senses when paddles are prevented from complete rotation and automatically returns to starting position to start over

• Features an integrated waste drawer for efficient cleaning and disinfection

• Easily remove paddles without any tools for quick cleaning

Description Cat. No. EachSmasher Blender 97059-208 3986.34

DescriptionThickness, mil Capacity

Dimensions, W x H, cm (in.) Cat. No. Price

For 80 Biomaster Stomacher BlendersStandard Bag with Tear-Off Top 3 207 mL (7 oz.) 10.2 x 15.2 (4 x 6) 11216-898 Cs. 1000/ 78.78For 400C Stomacher BlendersFILTRA-BAG with Safety Tab and Flat Wire Closure

3 710 mL (24 oz.) 15.2 x 22.9 (6 x 9) 89085-570 Cs. 400/ 243.91

FILTRA-BAG with Safety Tab and Flat Wire Closure

3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 89085-574 Cs. 400/ 291.55

FILTRA-BAG with Open Top 3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 11216-904 Cs. 400/ 411.35FILTRA-BAG with Tear-Off Top 3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 89085-572 Cs. 400/ 282.23SECURE-T Bag with Open Top 3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 89085-564 Cs. 1000/ 122.79SECURE-T Bag with Tear-Off Top and Write-On Strip

3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 89003-820 Pk. 250/ 52.85

SECURE-T Bag with Tear-Off Top 3 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 11216-900 Cs. 1000/ 164.25SECURE-T Bag with Tear-Off Top 4 1.63 L (55 oz.) 17.8 x 30.5 (7 x 12) 11216-902 Cs. 1000/ 240.47SECURE-T Bag with Tear-Off Top 4 4.25 L (144 oz.) 30.5 x 35.6 (12 x 14) 89085-562 Cs. 500/ 194.33For 3500 Series Stomacher BlendersSECURE-T Bag with Open Top 3 12.0 L (405 oz.) 38.1 x 50.8 (15 x 20) 89085-566 Cs. 250/ 186.45SECURE-T Bag with Tear-Off Top 3 12.0 L (405 oz.) 38.1 x 50.8 (15 x 20) 11216-890 Cs. 500/ 233.76SECURE-T Bag with Tear-Off Top 4 12.0 L (405 oz.) 38.1 x 50.8 (15 x 20) 11216-892 Cs. 500/ 297.54

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Page 20: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

soxtherm Rapid Extraction Units, Gerhardt• Ideal for fast, reliable determination of fat content in food products or pollutants in environmental samples• Multistat control unit can be used to control and monitor up to four individual extraction units• In case of malfunction, the extraction process is suspended at once and the beakers are lifted off the hotplate• Alphanumeric names can be assigned to stored programs for compilation of a comprehensive program library• All solvent types may be used and an over temperature cutout prevents the flash point of the particular solvent from being

exceeded• Maximum beaker volume: 140mL (4.7oz.)• Maximum temperature: 300°C (572°F)

Description Dimensions, W x D x H, cm (in.) Electrical Cat. No. EachControl Unit 18 x 26 x 12.5 (7

1/8 x 10 1/4 x 4

7/8) 115V, 50/60Hz, 6W 14236-102 4320.00Control Unit 18 x 26 x 12.5 (7

1/8 x 10 1/4 x 4

7/8) 230V, 50/60Hz, 6W 14236-104 4320.00Extraction UnitsTwo-Place 25 x 41 x 58 (9

7/8 x 16 1/8 x 22

7/8) 115V, 50/60Hz, 400W 14236-080 9810.00Two-Place 25 x 41 x 58 (9

7/8 x 16 1/8 x 22

7/8) 230V, 50/60Hz, 400W 14236-084 9810.00Four-Place 40.5 x 41 x 58 (16 x 16

1/8 x 22 7/8) 115V, 50/60Hz, 800W 14236-088 16,020.00

Four-Place 40.5 x 41 x 58 (16 x 16 1/8 x 22

7/8) 230V, 50/60Hz, 800W 14236-092 16,020.00Four-Place, Micro 40.5 x 41 x 58 (16 x 16

1/8 x 22 7/8) 115V, 50/60Hz, 800W 14236-086 16,020.00

Four-Place, Micro 40.5 x 41 x 58 (16 x 16 1/8 x 22

7/8) 230V, 50/60Hz, 800W 14236-090 16,020.00Six-Place 56.5 x 41 x 58 (22

1/4 x 16 1/8 x 22

7/8) 115V, 50/60Hz, 1200W 14236-096 23,040.00Six-Place 56.5 x 41 x 58 (22

1/4 x 16 1/8 x 22

7/8) 230V, 50/60Hz, 1200W 14236-100 23,040.00Six-Place, Micro 56.5 x 41 x 58 (22

1/4 x 16 1/8 x 22

7/8) 115V, 50/60Hz, 1200W 14236-094 23,040.00

Protein determination for meat and meat Products using the Kjeldatherm and vapodest unitsgerhardt

IntroductionThe percent of protein contained in a sample can be determined by digesting the organically bound nitrogen content of the sample with concentrated sulphuric acid, potassium sulphate, and a catalyst. Thus the nitrogen is broken down to ammonium sulphate. By adding caustic soda in excess, ammonia is released through water steam distillation and then trapped in a solution of boric acid. Finally, the solution is titrated against an acid solution to determine the percent of available nitrogen and then calculated to obtain the final protein determination.

Analysis1. Sample PreparationThere should be a representative sample of at least 200g to start, since it will generally need to pass through a blender or a mill at least twice to achieve a homogenous mixture. Once a homogenous mixture is achieved, the sample should be stored hermetically in order to avoid any changes or decay of the composition. Analysis of the prepared sample must be done within 24 hours. Prior to the analysis, the sample should be at room temperature.

When ready to run the analysis, prepared samples are weighed using weighing paper. After weighing, the samples and chemicals are added to the digestion tubes. Sulphuric acid is then used to wash down any sample residue that might remain at the glass walls.

The values given in the annex can be used as a guideline for the initial weighings. If samples are very homogenous, the initial sample weight can be set at the lower boundary of the suggested initial weight.

2. Digestion with Kjeldatherm Digestion SystemWhen working with a Kjeldatherm System and 250mL Kjeldatherm digestion tubes, it is recommended to set the digestion at 400°C (752°F) for 70 minutes. Digestion tubes should be put into the preheated block until the sample turns translucent.

Foaming during the digestion is to be expected, however, the foaming should not exceed two-thirds of the glass. If excessive reactions occur, take out the insert rack. During the digestion, black particles remaining at the glass walls should be washed back by condensing sulphuric acid. The sample glass must be translucent after the digestion in order to obtain good results.

3. Suction of the Digestion GasesDuring the entire digestion period the Turbosob scrubber should be working. About 1200mL of a 15% caustic soda solution is recommended for the washing bottle, as this amount is sufficient to neutralize digestion gases from approximately 60 digestions. During the cooling period (about 30 minutes after lifting the insert rack or turning off the heat) the scrubber should continue working.

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Page 21: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Protein determination for meat and meat Products using the Kjeldatherm and vapodest units (continued)

4. DistillationAfter the digested sample has cooled down, water steam distillation is effected according to the following program:

VAP 10/s VAP 20 VAP 30 VAP 45 VAP 50sH2O Addition 100 mL* 100 mL* 9 s 9 s 90 mLNaOH Addition 80 mL 8 s 8 s 8 s 80 mLDistillation Time 240 s 240 s 240 s 240 s 240 sSteam Power 100% 100% 100% 100% 100%Suction Sample manual manual 25 s 25 s 25 sH3BO3 Addition 50 mL 50 mL 50 mL 7 s 70 mLSuction Receiver manual manual manual manual 25 sTitration manual manual manual manual automaticCalculation manual manual manual manual automatic*All activities that have to be done manually are written in italic print.

5. TitrationIf using the VAP 50s model, titration will be automatic. If using one of the other Vapodest systems, 3-4 drops of an indicator mixture should be added to the distillate, which should then be titrated with a standard solution until the color changes. If the endpoint determi-nation is done with a pH meter or a titrator, the indicator mixture is not necessary.

6. Blank ValueThe blank value is determined by running all steps in the analysis with just the given chemicals. The sample should not be used at this point. When the calculation is done, remember to take into account the chemicals used to determine the blank value.

Validation1. Calculation

%N = 1.4007 * c * (V-Vb)Sample Weight (g)

c: Concentration of the standard acid solution (hydrochloric acid = 0.1mol/L or sulphuric acid = 0.05mol/L)

V: Consumption of the standard acid in mL during the sample analysis

Vb: Consumption of the standard acid in mL during the blank value analysis

%Raw Protein = %N * 6.25

2. Repeatabilityr = 0.35g/100g, Fried Pork Sausage

r = 0.26g/100g, Boiled Sausage

r = 0.18g/100g, Liver Sausage

3. ComparabilityR = 0.60g/100g, Fried Pork Sausage

R = 0.38g/100g, Boiled Sausage

R = 0.43g/100g, Liver Sausage

4. Analysis CheckThe reliability of the method can be done using the same sample material and determining the protein content in a ring test. The reli-ability and comparability can be obtained in this manner as well. In addition, such numbers can be obtained at:

National Institute of Standards & Technology (USA)

British Chemical Standards (UK)

Sample TableTheoretical Content, Amount of Sample, % Protein Type of Sample g (+/- 10 %) 82.10 – 87.10 Gelatin 0.5053.10 – 60.00 Meat Extract 0.5029.60 – 47.60 Binden, Meat 1.0025.30 Corned Beef, American 1.5022.40 – 25.20 Turkey, Breast without 1.50 Skin 17.50 – 25.10 Corned Beef, German 1.5012.00 – 25.00 Pork, Ham, Raw, 1.20 Smoked 22.70 – 24.80 Pheasant, with Skin, 1.50 without Bone 19.50 – 24.00 Pork, Muscle Only 1.5014.00 – 23.60 Sausage, Plockwurst 0.7021.70 – 23.40 Venison, Back 1.5021.40 – 23.30 Turkey, Young with Skin 1.5020.00 – 23.00 Hare 1.5022.90 Beef, Miced Meat 1.5020.60 – 22.70 Beef, Muscle Only 1.5022.15 – 22.60 Quail, without Skin 1.50 and Bone 22.50 Pork, Ham, Cooked 1.5022.45 Beef, Sirloin Roast 1.5022.40 Pork, Minced Meat 1.2022.20 Chicken, Breast with 1.50 Skin 22.20 Pork, Leg (Hind Leg) 1.5018.50 – 22.20 Pork, Loin, Cured 1.50 (Kassel) 18.10 – 22.20 Venison 1.5022.10 Chicken, Liver 1.5022.00 Pork, Fillet 1.5019.10 – 22.00 Beef, Top Round 1.5017.40 – 21.90 Ox, Spleen 1.5020.50 – 21.80 Minced Meat 1.50 (Steak Tartar) 21.75 Beef, Silverside 1.5021.20 – 21.70 Venison, Haunch (Leg) 1.5021.00 – 21.70 Sheep, Liver 1.5018.10 – 21.70 Horse, Meat 1.5021.60 Pork, Chop with Bone 1.5021.45 Beef, Rump 1.5021.40 Sausage, Landjäger 0.70

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wet analysIsDigestion | Distillation and Dilution

Page 22: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Vapodest Distillation systems, Gerhardt• Programming includes automatic NaOH addition and

variable steam power from 40 to 100%• All functions are continuously monitored via the

microprocessor and displayed on the digital display• Manual intervention is possible at any time during the

distillation process• Energy-saving stand-by mode automatically switches on

when the unit is not in use, maintaining full steam power, while minimizing the waste of cooling water

• Units will accept either 250–750mL Kjeldahl flasks with wide-neck openings or 100–800mL Kjeldatherm digestion tubes

• Model 20s is recommended for laboratories with low sample throughput

Vapodest distillation systems provide rapid steam distillation of Kjeldahl digestion solutions and volatile acids. It can also be used for alcohol determination or for physical separation of water-soluble substance.

F cusnformation

• Model 30s fulfills all requirements for Kjeldahl chemical additions, including automatic addition of water and aspiration of sample residue

Description Cat. No. EachVapodest 20s Distillation System 14214-440 9540.00Vapodest 30s Distillation System 14214-442 11,340.00

Kjeldatherm Block Digestion systems, Gerhardt• Run multiple digestions simultaneously for use in many applications• Includes an external controller to allow flexibility of placing system

in or out of a fume hood• Aluminum block features holes for digestion tubes and each

aluminum insert rack has two insulated handles on the side• Insert racks are available for tube sizes of 100, 250, and 400mL• Rack and exhaust manifold can be stored safely above the block

during cooling-down periods• Basic units feature a manual lift to handle the rack and manifold;

automatic units feature a lift motor• Basic units come with a TR controllor; for automatic programming a

TZ electronic temperature controller is available separately• Automatic models come with a TZ controller that allows up to nine

different programs to be defined and stored

Description For Tube Volume Cat. No. EachBasic Digestion Systems8-Place System 250 mL, Macro 14214-444 6120.00

8-Place System*400 mL, Special, Diminished

14214-446 7020.00

20-Place System 250 mL, Macro 14214-448 10,800.0040-Place System 100 mL, Micro 14214-450 11,700.00Automatic Digestion Systems8-Place System 250 mL, Macro 97040-120 10,260.00

8-Place System*400 mL, Special, Diminished

97040-122 10,800.00

20-Place System 250 mL, Macro 97040-126 14,760.0040-Place System 100 mL, Micro 97040-128 15,300.00*These models are especially suited for samples prone to excessive foaming.

Digestion tubes, labconco®

• Precisely controlled height and wall thickness ensure consistent fit in exhaust system

• Borosilicate glass tubes feature heavy-duty retention lip and contoured bottom for optimum heat transfer

• Volumetric tubes feature markings for accurate dilutions and built-in constriction for maximum refluxing and digestion speed

• Volume: 250mL

Description Cat. No. Pack ofStandard Straight Tubes 51001-408 5/ 215.00Standard Straight Tubes 51001-441 25/ 1055.00Volumetric Tubes 51001-204 5/ 254.00Volumetric Tubes 51001-248 25/ 1140.00

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wet analysIsDigestion | Distillation and Dilution

Page 23: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Corning® Milk Dilution Bottles• For dilution of milk samples prior to plating• Made of borosilicate glass• Graduated bottles have mark at 99mL for easy measuring• Include black phenolic screw cap with rubber liner that

will withstand repeated sterilization cycles• Capacity: 160mL (5

3/8oz.)

Description Cap Size Cat. No. Case of 48Narrow-Mouth BottlesPlain Bottle 28-400 16163-004 252.97Graduated Bottle 28-400 16163-026 347.39Wide-Mouth BottlesPlain Bottle 40-400 16163-059 324.90Graduated Bottle 40-400 16163-070 413.26

nalgene® Dilution Bottles, thermo scientific• Transparent polysulfone bottles are appropriate for most aqueous and many

organic compounds• Feature graduations at 90 and 99mL for easy preparation of 1/10 or 1/100 dilutions in

the same bottle• Bottles can withstand temperatures from –100 to 165°C (–148 to 329°F) and are

guaranteed leakproof• Included linerless polypropylene caps can withstand temperatures from 0 to 135°C

(32 to 275°F)

Description Capacity, mL (oz.) Cap Size Cat. No. Case of 48Narrow-Mouth Bottles 200 (6

3/4) 28-415 16161-803 379.69Wide-Mouth Bottles 205 (7) 38-415 16161-847 445.14

VWR® Zahn Cup-type Viscometers• Use to measure viscosity of very thin to very thick liquids• Measure viscosity in Zahn seconds, which is the amount of time required for a

steady stream of liquid to flow from the hole in the bottom of the cup• Constructed of corrosion-resistant stainless steel

Viscosity Range, sec.Kinematic Viscosity Range, centistokes at 250°C Cat. No. Case of 50

Very Thin 45–80 18–56 82027-612 5603.50Thin 25–80 40–230 82027-614 5603.50Medium 20–75 150–790 82027-616 5603.50Heavy 20–80 220–1100 82027-618 5603.50Very Heavy 20–75 460–1725 82027-620 5603.50

VWR® Viscometer with Pear-shaped Bulbs• For determining relative viscosities by measuring flow

time through a standard capillary• Pear-shaped bulbs provide a gradual slope for liquid

delivery along the sides and entrances• Adjusted to give delivery time of 80–100 seconds for

water at 20°C (68°F)• Minimum sample volume: 2mL

Description Cat. No. EachViscometer with Pear-Shaped Bulbs 66044-007 208.91

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wet analysIsDistillation and Dilution

dId you Know...

The vwr Collection offers a wide selection of glassware for all of your QA/QC needs. visit vwr.com or contact your vwr sales representative for more information.

Page 24: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

analysette® 3 Vibratory sieve shakers, Fritsch• High-tech vibratory sieve shakers are ideal for quantitative particle size analysis of

solids and suspensions• Shakers can be used for either wet or dry sieving• Feature a precise digital timer and optical display of amplitude• Both models come with AutoSieve for Windows™

• PRO model shaker permits automatic amplitude evaluation and regulation of the sieving process

• The PRO model can be used as a calibration and validation inspection instrument, as well as for quality management according to ISO 9001:2000

• Sieves and other accessories are supplied separately; contact your VWR Sales Representative for more information

• Manufacturer’s two-year warranty• CE marked

Description Cat. No. EachSPARTAN Vibratory Sieve Shaker 89033-424 3511.00PRO Vibratory Sieve Shaker 89033-422 4703.00

Mini-UniPrep™ syringeless Filters, Whatman™

Quickly and Easily Remove Particulates from HPLC Samples• Filters are ideal for use in routine analysis, composite assays, content uniformity,

protein precipitation, and solubility and dissolution testing• Depth polypropylene (dp Polypropylene) membranes are recommended for aqueous

or organic solvents• Glass microfiber (GMF) membranes are best for particulate-laden liquids• Nylon membranes work best with aqueous or organic solvents with a pH range of

3–10• PES membranes should be used with biological samples requiring low protein

binding media• Polypropylene membranes are for general filtration and solvent-based samples• PTFE membranes are recommended for chemically aggressive samples• PVDF membranes should be used for aqueous solutions that require low

nonspecific protein binding• Filters with slit PTFE/silicone septa are ideal for high throughput automation and

for instruments with sensitive sampling needs

F cusnformation

Shakers operate with a magnetic drive in the base of the instrument that vibrates the stack vertically. The regulated vertical action produces optimum material sizes for sieving and ensure an even distribution across the sieve surface.

Pore Size, µm

Membrane Material Cat. No. Pack of

Amber Filters with Standard Cap0.2 Nylon 84009-506 100/ 329.810.2 PES 84009-510 100/ 329.810.2 Polypropylene 84009-512 100/ 329.810.2 PTFE 84009-508 100/ 329.810.2 PVDF 84009-504 100/ 329.810.45 dp Polypropylene 84009-516 100/ 329.810.45 GMF 83009-798 100/ 329.810.45 PES 83009-804 100/ 329.810.45 Polypropylene 83009-806 100/ 329.810.45 PTFE 83009-802 100/ 329.810.45 PVDF 84009-514 100/ 329.81Translucent Filters with Standard Cap0.2 Nylon 14224-976 100/ 294.480.2 Nylon 14224-730 1000/ 2438.290.2 PES 14224-914 100/ 294.480.2 PES 14224-746 1000/ 2438.290.2 Polypropylene 14224-930 100/ 294.480.2 Polypropylene 14224-762 1000/ 2438.290.2 PTFE 14224-946 100/ 294.48

Pore Size, µm

Membrane Material Cat. No. Pack of

0.2 PVDF 14224-794 1000/ 2438.290.2 PVDF 14224-978 100/ 294.480.45 dp Polypropylene 28137-750 100/ 294.480.45 dp Polypropylene 28137-752 1000/ 2354.890.45 GMF 28137-770 100/ 294.480.45 GMF 28137-772 1000/ 2438.290.45 Nylon 28137-754 100/ 294.480.45 Nylon 28137-756 1000/ 2438.290.45 PES 28137-776 1000/ 2438.290.45 Polypropylene 28137-766 100/ 294.480.45 Polypropylene 28137-768 1000/ 2438.290.45 PTFE 28137-758 100/ 294.480.45 PTFE 28137-760 1000/ 2438.290.45 PVDF 28137-762 100/ 294.480.45 PVDF 28137-764 1000/ 2438.29Translucent Filters with Slit Septa Cap0.2 Nylon 12000-526 100/ 363.020.2 Nylon 12000-536 1000/ 2540.020.2 PES 12000-530 100/ 363.020.2 PES 12000-540 1000/ 2540.02

Pore Size, µm

Membrane Material Cat. No. Pack of

0.2 Polypropylene 12000-532 100/ 363.020.2 Polypropylene 12000-542 1000/ 2540.020.2 PTFE 12000-528 100/ 363.020.2 PTFE 12000-538 1000/ 2540.020.2 PVDF 12000-524 100/ 363.020.45 dp Polypropylene 83009-810 100/ 363.020.45 dp Polypropylene 83009-824 1000/ 2540.020.45 GMF 83009-812 100/ 363.020.45 GMF 83009-826 1000/ 2540.020.45 Nylon 83009-814 100/ 363.020.45 Nylon 83009-828 1000/ 2540.020.45 PES 83009-818 100/ 363.020.45 PES 83009-832 1000/ 2540.020.45 Polypropylene 83009-820 100/ 363.020.45 Polypropylene 83009-834 1000/ 2540.020.45 PTFE 83009-816 100/ 363.020.45 PTFE 83009-830 1000/ 2540.020.45 PVDF 83009-808 100/ 363.020.45 PVDF 83009-822 1000/ 2540.02

Ge Healthcare life sciences

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wet analysIsfiltration

Page 25: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

sentino™ Microbiology Pump, Pall life sciences• Use to analyze aqueous-based fluids

for microbial contamination• Small footprint provides flexibility in

arranging workspace• Soft-touch keypad provides simple

on/off and pulse features to control filtration rate

• Peristaltic flow design pulls sample through the filter and fluid path, eliminating the need for a vacuum source

• Meets Membrane Filter (MF)

VWR® Vacuum Filtration systems• Incorporates a large membrane area

and low center of gravity for fast sample processing

• Funnel base and cap feature convenient finger tabs

• Nylon membrane is suitable for nonaqueous solutions

• Polyethersulfone (PES) membrane offers fast liquid flow rates and low protein binding

• Sterilized by gamma irradiation

Capacity, mL Cat. No. Pack of 12Systems with 0.2 μm Nylon Membrane150/ 250 87006-074 120.33500 87006-076 139.821000 87006-078 176.26Systems with 0.45 μm Nylon Membrane150/ 250 87006-080 120.33500 87006-082 139.821000 87006-084 176.26Systems with 0.2 μm PES Membrane150/ 250 87006-062 107.80500 87006-064 137.081000 87006-066 176.26Systems with 0.45 μm PES Membrane150/ 250 87006-068 120.33500 87006-070 139.821000 87006-072 176.26

acrodisc® syringe Filters with supor® Membrane, Pall life sciencesProvides Powerful Performance Advantages• Low protein binding minimizes sample loss• Sterilized by gamma irradiation to eliminate potential contamination by EtO residuals

F cusnformation

technique filtration parameters

Description Cat. No. PriceSentino Microbiology Pump 89183-082 Ea./ 1200.00Disposable Fluid Path 89183-086 Pk. 10/ 80.00

Supor membrane is a hydrophilic polyethersulfone material that is ideal for cell culture and biological research. The membrane offers superior flow rates and higher throughput over competitive devices, while the built-in pre-filter option offers even better throughput of difficult-to-filter liquids.

Pore Size, µm Color Cat. No. Pack of13 mm Dia. Acrodisc Syringe Filters0.2 Blue 28143-300 75/ 159.600.45 Clear 28143-302 75/ 159.600.8 Green 28143-304 75/ 162.5825 mm Dia. Acrodisc Syringe Filters0.1 Red 28143-309 50/ 125.650.2 Blue 28143-310 50/ 125.650.45 Clear 28143-312 50/ 125.650.8 Green 28143-314 50/ 125.650.8/0.2 Blue/Green 28144-009 50/ 155.2432 mm Dia. Acrodisc Syringe Filters0.1 Red 28150-957 50/ 147.800.2 Blue 28143-350 50/ 147.800.45 Clear 28143-352 50/ 147.800.8/0.2 Blue/Green 28139-702 50/ 177.351.2 Red 28150-958 50/ 147.805 Green 28150-956 50/ 147.80

orderIng ProduCts Is easy through vwr.Com

our web site gives you maximum control over the ordering process. with all of the available options, you can simplify your purchasing process by matching it to your own business practices.

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wet analysIsfiltration

Page 26: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

VWR signature™ Ergonomic High Performance single-Channel Variable Volume Pipettors• Superior accuracy and precision for critical applications• Significantly reduced plunger forces• Volume ranges from 0.2µL to 10mL• Fully autoclavable• Micro starter kit contains three pipettors in sizes 0.2–2µL, 0.5–10µL, and 10–100µL;

two racks of VWR Signature pipettor tips; and a three-position shelf clip• Promo starter kit contains four pipettors with tip ejectors in sizes 0.5–10µL, 2–20µL,

20–200µL, and 100–1000µL; three racks of Axygen pipettor tips; and a six-position linear stand• Standard starter kit contains three pipettors in sizes 2–20µL, 20–200µL, and 100–1000µL;

two racks of VWR Signature pipettor tips, and a three-position shelf clip

Description Cat. No. EachMicro Starter Kit 89080-002 769.04Promo Starter Kit 89080-004 935.55Standard Starter Kit 89080-000 728.42

VWR® Ultra High-Performance Pipettors• Soft-plunger system ensures light aspiration and dispensing forces• Levered ejection mechanism is designed to provide smooth and gentle tip ejection• Four-digit micrometer allows for fine control over volume adjustment and volume adjustment wheel can be locked to prevent

accidental volume changes• Handle is constructed of high-strength polypropylene polymer that can be autoclaved• Supplied with a self calibration tool for in lab calibration when used with a balance• Starter kit includes one of each of size 0.5–10, 2–20, 20–200, and 100–1000µL pipettors; a four-place linear pipettor stand; and

one rack each of size 10µL, 200µL, and 1000µL VWR starter tips

Volume Range, µL Accuracy, % Precision, % Cat. No. EachStarter Kit — — 89133-288 1037.950.1–2.0 ±12.0–1.5 ≤6.0–0.7 89130-552 315.420.5–10 ±4.0–0.5 ≤2.8–0.4 89130-554 315.422–20 ±3.0–0.8 ≤1.5–0.3 89130-556 309.875–50 ±3.0–0.8 ≤2.0–0.4 89130-558 309.8710–100 ±1.6–0.8 ≤0.8–0.2 89130-560 309.8720–200 ±1.2–0.6 ≤0.6–0.2 89130-562 309.87100–1000 ±0.9–0.6 ≤0.4–0.15 89130-566 309.87

VWR® Disposable transfer Pipets• One-piece, unbreakable pipets are nontoxic and inert• Can be sealed and refrigerated or frozen in liquid nitrogen• Small tips ensure uniform drop size• Sterile

Capacity, mL

Maximum Graduation Volume, mL

Graduations, mL

Overall Length, cm (in.) Packaging Cat. No. Case of

Large Bulb Pipets3 2 1 15.2 (6) Individually Wrapped 414004-037 500/ 118.115.8 1 0.25 15.5 (6

1/8) Individually Wrapped 414004-016 4000/ 475.045.8 1 0.25 15.5 (6

1/8) Peel-Off Pouch (50 x 10) 414004-040 5000/ 538.495.8 1 0.25 15.5 (6

1/8) Bulk Packaged 414004-015 5000/ 461.047.5 3 0.5 15.5 (6

1/8) Individually Wrapped 414004-043 5000/ 672.067.5 3 0.5 15.5 (6

1/8) Peel-Off Pouch (50 x 10) 414004-045 5000/ 679.407.5 3 0.5 15.5 (6

1/8) Peel-Off Pouch (100 x 5) 414004-044 5000/ 679.407.5 3 0.5 15.5 (6

1/8) 20 per Bag 414004-013 5000/ 382.74Thin Stem Pipets1.5 0.9 0.1 11.6 (4

9/16) Individually Wrapped 414004-024 4000/ 479.171.5 0.9 0.1 11.6 (4

9/16) 20 per Bag 414004-025 5000/ 441.37

find Complete product listings at vwr.com or Call 1.800.932.5000 | 26 26 | The EssEntials of Quality Assurance & Quality Control find Complete product listings at vwr.com or Call 1.800.932.5000 | 27

wet analysIsliquid handling

Page 27: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

Corning announcesA new brand of labware optimized for quality control labsa complete range of products to meet your needs from sampling to disposal• Compliance with international quality standards• Gosselin FDA registered - Raw materials/resins, compliancy with (94/62/EC) - ISO 9001: 2008 certified• Easily accessible traceability features such as:

• Peelable labels with lot numbers, valid expiration date and sterilization information to stick in your laboratory notebook• Sterilization indicator on each case• Certificates of Compliance are available for download

Sampling Inoculation Grinding & Homogenization Incubation Liquid Transfer Disposals

new vwr PIPet tIP seleCtor guIde onlIne

search by pipettor brand or desired tip attributes and receive instant recommendations on the best tips to fit your application needs. visit the liquid handling program area on vwr.com to try it out today!

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wet analysIsliquid handling

Page 28: VWR The Essentials of QA/QC Brochure, Lit. No. 92933

1111 25M Lit. No. 92933

The vwr CollectionHigh Quality. Superior Performance. Competitive Pricing.

The VWR Collection is an

exclusive line of products

that delivers the quality and

value you’ve come to trust

from VWR. Whether you

need equipment, instruments,

consumables, or chemicals,

there’s a VWR Collection

product for your lab...and your

budget. Learn more about the

VWR Collection by contacting

your VWR Sales Representative,

visiting vwr.com, or calling

1.800.932.5000 today!

look inside for:VWR® symphony™ Low-Temperature B.O.D. Incubations (page 17), VWR® Microcentrifuges (page 10), VWR® VistaVision™ Planachromatic Compound Microscope with Triangular Head (page 15), VWR® Signature™ Ergonomic High Performance Single-Channel Variable Volume Pipettors (page 26)

of Quality Assurance & Quality ControlEssEntialsTh

e

ATTn: mAilroom:

If addressee is no

longer with the

organization, please

deliver to laboratory

products buyer.

Thank you.

vwr lIt lIBraryc/o Archway20770 westwood Drivestrongsville, oh 44149

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