WATERS ADVANCED PURIFICATION COLUMNS · reduce flow to 0 mL/min, Adjust the inlet connector until the void is eliminated. (If this is unsuccessful, see “Eliminating Voids”). 3

[ CARE AND USE MANUAL ]

Waters Advanced Purification Columns 1

CONTENTS

I. INTRODUCTIONa. AP Column Accessories

II. USING THE COLUMN a. Safety and Solvent Considerations

b. Adjusting the Plunger

c. Packing the Column

d. Installing the Column

e. Equilibrating the Column

f. Eliminating Voids

III. CARE AND MAINTENANCEa. Troubleshooting

b. Replacing Ferrules

c. Disassembling and Reassembling the Inlet Connector

d. Replacing End Fittings

e. Replacing Filters and O-rings

f. Storing the Column

IV. SCALING UP SEPARATIONSa. Scaling a Seperation

b. Adjusting Sample Load and Flow Rate

c. Adjusting Gradient Parameters

d. Adjusting Gradient Delay Volume

V. ORDERING INFORMATION

VI. MISCELLANEOUS

WATERS ADVANCED PURIFICATION COLUMNS

I. INTRODUCTION

Waters® Advanced Purification (AP) series of glass columns are

constructed of biocompatible glass and polymeric materials and

can be easily used with silica, polymer, or soft gel packings. To

optimize flow and ensure uniform sample distribution onto the

packed bed, each column incorporates a distributor. A replaceable

filter protects the packing from large particulate contaminants.

Empty AP glass columns are available in a variety of sizes and

utilize the same design to ensure predictable methods transfer

among them. AP glass columns are compatible with both analytical

and preparative HPLC and FPLC systems.

Figure 1: Advanced Purification Column

Outlet tubing

Outlet filter

Outlet connectorassembly

Glass column

Inlet connector assembly

Inlet tubing

Seal adjustment nut

Inlet filter

Plunger

Inlet cap

Fine adjustment knob

Coarse/fineadjustment slide



a. AP Column Accessories

The following accessories are shipped with the AP column:

• Packing funnel

• End plugs for column storage

• Spare Parts Kit consisting of:

- 1 inlet O-ring

- 1 outlet O-ring

- 1 connector tip O-ring

- 1 packing funnel O-ring

- 2 filter assemblies

- 2 ferrules

- 10 gripper ring tools

- 1 Allen wrench

Table 1: AP Column Specifications

II. USING THE COLUMN

a. Safety and Solvent Considerations

Inherent surface imperfections can weaken glass at high pressures.

Maintain lower pressures to prolong column life. The AP glass

column has an external plastic jacket which acts as a safety shield.

To avoid column hardware damage, never exceed 10 MPa (1500 psi

or 100 atm) inside the column.

High backpressures may damage resin-based packing materials.

Consult your packing material care and use manual for packing

material backpressure limits.

AP column materials are compatible with most solvents used in

HPLC. However:

• Certain solvents, such as acetone or chlorinated hydrocarbons,

may reduce the life expectancy of the O-rings.

• The transparent plastic jacket may weaken if exposed to certain organic

solvents. If the jacket crazes, replace it.

b. Adjusting the Plunger

Range of movement

The packed bed is at maximum volume when the hex at the inlet end

of the plunger is flush with the top of the coarse/fine adjustment

slide. Do not withdraw the plunger further, or too few threads will be

engaged to ensure reliable operation.

The slide must be engaged against the locking hex while the column

is in use to prevent the plunger from unscrewing under pressure.

Figure 2: Column at Maximum Volume

Internal diameter 10 mm

Length (nominal) 100, 200, 300, or 600 mm

Maximum operating pressure 10 MPa (100 Atm, 1500 psi)

Filter size 20 µm

Materials along fluid path: Column Tubing Filter Connectors O-rings

Borosilicate glassTefzel®

PolyethyleneRyton-4® (polyphenylene sulfide resin)Buna-N

Operating temperature 4 °C to 50 °C

Correct Incorrect Incorrect Incorrect

Inlet Hex is Flush with Top of Slide

ThreadsExposed

Locking HexExposed

Inlet HexExposed



Using the coarse/fine adjustment slide

The AP column features a coarse/fine adjustment slide to make

plunger adjustment fast and easy. Make sure the slide is in the down

position (against the fine adjustment knob) before pressurizing the

column, Figure 3 shows:

1. Deactivating the inlet O-ring seal

2. Using the slide for coarse adjustment

3. Using the slide for fine adjustment and locking

The slide must engage the locking hex during operation to

prevent the inlet plunger from unscrewing under pressure.

4. Activating the O-ring seal

Figure 3: Adjusting the Plunger Downward

c. Packing the Column

The Waters AP Column is shipped unpacked. The AP Column is

compatible with all commonly used silica- and resin-based packing

materials. Repack the column when performance deteriorates or

when a new method requires a different packing material.

When you repack an AP Column, empty the column first (see

“Emptying the Column”), then proceed to the correct section for

slurry-packing or dry-packing the column.

Column volumes

When you pack the column, you must calculate the column volume

from the length of the packed bed you will use in your method.

Column volumes are:

Short beds

To pack a column with a shorter bed, replace the outlet connector with

a second inlet connector (see Section V, “Ordering Information”). Inlet

connectors have a 40 mm range of movement, allowing you to reduce

column volume to as little as 3 mL. The minimum bed length for a

100 mm long column with two inlet connectors is 20 mm.

Slurry-packing the column

Amount of packing material required

To determine the amount of packing material required, multiply

the column volume, V (mL), by the bulk density of the packing

material, d (g/ml):

Amount of packing material required = V x d

Determining density

To determine the density of the packing material:

1. Slurry 1 g of packing material in 10 mL of buffer and allow the

material to settle.

2. Note the volume of the settled material

3. Calculate density by dividing the weight of the material by the

settled volume.

Column length Column volume

100 mm 8 to 8 mL

200 mm 13 to 16 mL

300 mm 21 to 24 mL

600 mm 45 to 48 mL

Packing Material Bulk Density of the Packing Material

Waters Protein-Pak™ HR 1.2 g/mL

Waters AccellPlus™ silica-based packing materials

0.5 g/mL

Other packing materials Use procedure below to determine density



Preparing slurry solutions

To make slurry solutions, suspend one part of packing material

in three parts of degassed solution. The packing funnel can hold

40 mL of slurry.

Slurry-packing

To slurry-pack the column (Figure 4):

1. Unscrew the inlet connector from the inlet cap.

2. Screw the packing funnel into the inlet cap.

3. Clamp the column upright to a rigid support. Drain the outlet

tubing to waste.

4. Fill the column with the slurry. If the slurry does not flow into

the column, insert a pipette into the tip of the funnel to break

the surface tension.

5. When all of the packing material is in the column, plug the

outlet tubing.

6. Let the packing material settle. Remove excess solvent from the

top of the bed with a pipette. Do not disturb the bed.

7. Remove the funnel, and reattach the inlet connector.

8. Adjust the inlet connector until the plunger contacts the packed bed.

Ensure that the packed bed does not exceed the nominal

column length.

Figure 4: Slurry-Packing the Column

Dry-Packing the Column

Amount of packing material required

To determine the amount of packing material required, multiply

the column volume, V (mL), by the bulk density of the packing

material, d (g/mL):

Amount of packing material required = V x d

Waters Protein-Pak HR must be slurry-packed (see “Slurry-packing

the column”)

Determining density

To determine the ulk density of the packing material, put 19 g of

packing material into a 10 mL graduated cylinder. Tap the graduated

cylinder on a flat surface until the material is completely settled.

Note the volume and calculate the density.

Dry-packing

To dry-pack the column:

1. Unscrew the fine adjustment knob from the inlet cap.

2. Screw the packing funnel into the inlet cap.

3. Remove the outlet tubing.

4. Holding the column vertically, gradually fill the column with packing

material. While filling, tap the column lightly on a flat surface until the

material settles.

5. Remove the funnel and reattach the inlet connector and the

outlet tubing.

6. Adjust the inlet connector until the inlet plunger contacts the

packed bed.

Ensure that the packed bed does not exceed the nominal

column length.

Packing Material Solution

Ion-exchange chromatography High concentration buffer

Reversed-phase chromatography Methanol

Hydrophobic-interaction chromatography (HIC)

Water or low concentration buffer

Slurry

Funnel

Inlet cap

To waste

Packing Material Bulk Density of Packing Material

Waters AccellPlus silica-based packing materials

0.5 g/mL

Other packing materials Use procedure below to determine density



Emptying the column

To remove packing material from the column:

1. Reverse the direction of flow through the column by connecting the

column outlet to the pump.

2. Remove the inlet connector by unscrewing the fine adjustment knob

from the inlet cap.

3. Screw the packing funnel into the inlet cap. Position a beaker under the

mouth of the funnel.

4. Start flow. Gradually increase the flow until the packed bed begins

to move. Continue flow until all packing material washes out of

the column.

5. Remove the funnel and clean the column. Make sure the threads

are clean.

6. Repack the column (see “Slurry-packing the column”, or “Dry-packing

the column”, for repacking instructions).

d. Installing the Column

The AP Column can be installed in:

• Waters 650 Advanced Protein Purification System

• Waters 625 system

• Pharmacia® FPLC® systems

• Other high performance liquid chromatography (HPLC) systems (refer

to Table 4 for adapters and unions used to connect the AP column to

selected systems)

Refer to the appropriate system user’s guide for specific column instal-

lation information.

Tightening fittings

Finger-tighten plastic fittings. Do not overtighten. Overtightening

may damage the threaded parts of the column. Overtightening does

not improve performance.

End plugs

Save the column end plugs for future column storage.

e. Equilibrating the Column

Before performing a test run, equilibrate the column:

1. Gradually increase the flow in 0.1 mL/min increments until you attain

the desired flow rate.

2. While equilibrating the column, check for voids; if present, gradually

reduce flow to 0 mL/min, Adjust the inlet connector until the void is

eliminated. (If this is unsuccessful, see “Eliminating Voids”).

3. Equilibrate the column with 10 to 20 column volumes of mobile phase,

or as recommended by the manufacturer of the packing material.

f. Eliminating Voids

Voids may develop in the column during normal use. To eliminate voids:

1. Stop flow.

2. Disconnect the inlet column tubing from the system to allow displace-

ment of fluid while adjusting the plunger.

3. Deactivate the O-ring seal by turning the seal adjustment nut

counterclockwise.

4. Move the slide to the fine (down) position and turn it slowly to

eliminate the void.

If the fine adjustment knob is all the way down, back if off a few turns.

Move the slide to the coarse adjustment position and move the plunger

down a short distance. To eliminate the void, return the slide to the fine

adjustment position and turn it slowly.



I I I . CARE AND MAINT ENANC E

a. Troubleshooting

Table 2: Troubleshooting the AP Column

b. Replacing Ferrules

Each of the four tubing connections on the AP column uses a gripper

ring between two washers (to keep the Tefzel tubing from sliding when

the column is under pressure) and a ferrule. This section explains how

to set the gripper ring and replace a ferrule on the AP column.

Installing the Ferrule, gripper ring, and washer assembly

To install a new ferrule, gripper ring, and washer (Figure 5):

1. Slide a washer over the end of the tubing.

2. Place a gripper ring in the depression on the gripper ring tool.

3. Insert the end of the tubing into the gripper ring tool as far as it will

go (see Figure 5). This seats the gripper ring at the proper distance

from the end.

4. Withdraw the tubing from the gripper ring tool and slide another washer

over the end of the tubing.

5. Slide the ferrule over the end of the tubing, flared end first.

Use l/I6-inch o.d., 0.009-inch i.d. Tefzel tubing for all connections.

Figure 5: Using the Gripper Ring Tool

c. Disassembling and Reassembling the Inlet ConnectorTo replace the ferrule at the connection between the inlet connector and

the column, you must first disassemble the inlet connector assembly.

Disassembling the inlet connectorTo disassemble the inlet connector assembly:

1. Deactivate the O-ring by turning the seal adjustment knob.

2. Move the slide to the coarse adjustment position and unscrew

the plunger.

3. Use a screwdriver to remove the O-ring located under the cover.

4. Unscrew the seal adjustment knob, exposing the set screw that holds

the core in the proper orientation. Use the Allen wrench from the Spare

Parts Kit to remove the set screw.

5. Push the core out of the plunger and unscrew the inlet connector tip.

6. Pull out the tubing. Use a single-edge razor to cut the tubing above

the washers. To replace the ferrule, follow the procedure

in “Replacing Ferrules”.

Reassembling the inlet connectorTo reassemble the inlet connector:

1. Insert the end of the tubing and the ferrule into the inlet connector tip.

Then slide the core over the tubing and screw it to the tip. Hand-tighten

the fitting.

2. Slide the plunger over the core and replace the set screw. The plunger

core has three slots and the plastic plunger has two threaded holes.

There is only one perfect match. Position the hex of the inlet connector

tip to align onwe of the slots with one of the threaded holes. If there is no

perfect match, tighten the inlet connector tip a little more.

Symptom Possible Cause Corrective Action

Poor resolution Sample overload Reduce sample amount.

Flow rate too high Reduce flow rate.

Packed bed void Adjust inlet connector to eliminate void.

High backpressure

Plugged filter Replace the filter (see “Replacing Filters and O-rings”).

Leakage into plastic shield or out of inlet connector

Foreign matter on sealing surfaces

Clean surfaces and re-tighten.

O-ring seal is not activated

Turn seal adjustment nut counter-clockwise to active O-ring.

Damaged O-rings Replace O-rings (see “Replacing Filters and O-rings”).

Gripper ring Gripper ring tool

Washer

FerruleWasher



3. Replace the set screw. Do not overtighten the set screw; the plunger

core must slide easily to permit the O-ring seal to form.

4. Reassemble the column.

d. Replacing End Fittings

The end fittings for the AP column are compression screws with

gripper rings, washers, and ferrules.

Making a new compression screw assembly

To make a new compression screw assembly:

1. Use a single-edge razor to make a straight, square cut on the plastic

tubing. Make the cut in front of the compression screw.

2. Slide a compression screw fitting and a washer over the end of

the cut tubing.

3. Place a gripper ring in the top depression of the tool (see Figure 5).

Insert the end of the tubing into the gripper ring tool as for as it will

go. This moves the gripper ring to the proper distance from the end of

the tubing.

4. Remove the tubing from the tool and slide the other washer and ferrule

over the end of the tubing.

5. Insert a union over the end fitting assembly and tighten it to seat the

end-fitting.

e. Replacing Filters and O-rings

The inlet and outlet connector assemblies each contain a filter.

Replace filters periodically, depending on:

• The cleanliness of the sample and solvents

• The type and particle size of the packing material

Distributors for AP filters are inside the filter skirt. Look for the

star-like pattern on the distributor surface to ensure the distributor

has not become dislodged.

The AP column contains three O-rings, one each at the connectors

and one at the filter. The outlet connector O-ring does not normally

need replacement.

Accessing the filters

To access the inlet filter:

1. Deactivate the inlet O-ring by turning the seal adjustment knob

counterclockwise.

2. Slide the inlet connector adjustment to the coarse adjustment position,

and unscrew the plunger from the column.

To access the outlet filter, unscrew the outlet connector from the plastic

shield and remove from the glass column.

Installing the filter

To install on inlet filter:

1. Remove the old filter. Pry off the filter with a fingernail (or by inserting

a blunt flat tool between the filter and shoulder of the connector tip).

2. Install the new filter as follows:

For AP-1 (refer to Figure 6)

Ensure that the Tip O-ring is in place on the connector tip. Snap the

new filter onto the connector tip.

For AP-2 & AP-5 (refer to Figure 7)

The Distributor has grooves on one surface and those grooves must

face the filter as shown in Figure 7. Each distributor has an insert on the

backside which contacts the connector. Should the insert fall out of the

Distributor, for consistency, simply re-install it with the circular marks

facing the Distributor as shown. Please note that acceptable factory

installation of the insert may be such that the circular marks are visible

and facing the connector

For AP-2 Columns, install the Distributor assembly into the Filter and

snap them onto the connector.

For AP-5 Columns, install the Distributor assembly into the connector

and snap the filter onto the connector.

Figure 6: Inlet Connector Tip for AP-1 Column

Filter assembly

Connectoro-ring

Connector(inlet shown)

Tip o-ring



Figure 7: Inlet Connector Tip for AP-2 and AP-5 Columns

Replacing an inlet connector O-ring

To replace the inlet connector O-ring:

1. Deactivate the inlet O-ring by turning the seal adjustment

knob. Remove the inlet connector assembly from the column

by unscrewing the plunger.

2. Use a small blunt tool, such as a push pin, to gently pry up the O-ring so

that you may roll it off the connector with your fingers. Carefully push

a new O-ring onto the connector tip. Be sure to select the proper inlet

connector O-ring.

Do not scratch the O-ring groove. If the groove is scratched, the

column will leak.

3. Return the inlet connector assembly to the column. Reactivate

the O-ring.

Replacing a filter O-ring

This small O-ring is located at the edge of the filter skirt. With the filter off, roll

the O-ring off the connector tip and replace it with a new one.

f. Storing the Column

Storage considerations

Store a packed glass column according to the recommendations of the manu-

facturer of the packing material. Note the following:

• Store the column with end plugs in place to prevent column drying.

• Use bacteriostatic solutions in appropriate concentrations, for example,

0.2% sodium azide or 30% ethanol.

• Remove all salts from the column before adding an organic solvent.

An organic solvent added to a salt can cause precipitation, resulting in

increased backpressure.

• Extended storage in high salt buffers may result in the formation

of microcrystals which can lead to high backpressure. (Dissolve

microcrystals by slowly introducing a low ionic strength buffer).

• Do not allow the mobile phase to freeze. Storage at 4 °C is acceptable.

IV. SCALING UP SEPARAT IONS

a. Scaling a Separation

When scaling an analytical separation to a preparative separation, use the

following isolation approach:

1. Define the objective by determining the:

• Complexity of the sample mixture

• Component to be isolated

• Required quantity

• Degree of purity

• Properties of the components in the mixture

2. Perform crude separations to remove large quantities of extraneous

material or to isolate groups or classes of compounds. Simplify a com-

plex sample through coarse separation techniques, such as liquid-liquid

or solid-liquid extraction, crystallization, or precipitation. Centrifuge or

filter the sample to remove particulates.

3. Develop the separation method. To optimize the separation for com-

pounds of interest, make small-scale injections of the sample mixture,

varying the gradient.

4. Perform a loading study. Make progressively larger injections of the

sample mixture on a small scale column, such as the AP-Minicolumn,

to determine the effect of overload on resolution. This study defines the

amount of sample that can be separated in a column of a given volume.

5. Scale up the separation. Translate the small scale parameters to the

large scale system values. Adjust the:

• Sample load

• Flow rate

• Gradient volume

Filter assembly

Connectoro-ring

Connector(inlet shown)

Distributor

Insert



b. Adjusting Sample Load and Flow Rate

When scaling the separation, use analytical and preparative columns

that are:

• The same length

• Packed with the same particle size material

This section describes three equations to adjust the sample load

and flow rate. Use Equation l to calculate the sample load and

Equation 2 to calculate the flow rate. Alternately, use Equation 3 to

calculate a scale factor, which you may use to obtain the preparative

value. In all three equations, if you use preparative columns and

analytical columns of the same length, both sample mass and flow

rate are proportional to the square of the column cross section.

In the following equations:

p = preparative column

a = analytical column

m = mass injected

I = column length

d = column diameter

F = flow rate

v = column volume

sf = scale factor

t = times in gradient table

Vd = delay volume of the instrument

Equation 1To increase sample load in proportion to the volume of the packed bed:

Equation 2To increase flow rate in proportion to the volume of the packed bed:

Equation 3 To calculate the scale factor:

Multiply the analytical sample mass and/or flow rate by the scale

factor to obtain the preparative value. You should obtain similar reten-

tion times and peak widths for both the analytical and the preparative

column and thus, similar resolution.

c. Adjusting Gradient Parameters

If you have scaled the flow rate in proportion to the column volume, the gradi-

ent time profile should remain constant. However, due to pressure limitations

on the preparative instrument or column, you may have to reduce the flow

and adjust the gradient time table. Use the following formula to

• Keep a constant ratio between the gradient volume and column volume

• Adjust the gradient table

d. Adjusting Gradient Delay Volume

Before the gradient reaches the inlet of the column, there is an isocratic period

caused by the delay volume between the point of mixing of the gradient and

the column inlet. The isocratic period exists until the delay volume is purged.

In small columns, the delay volume results in a longer isocratic period than

in larger preparative columns. To maintain the same separation for analytical

and preparative columns, you must delay the gradient for the preparative

column by the some number of column volumes. Use the following equation

to calculate the additional gradient delay time for the preparative column:



V. ORDERING INFORMAT ION

Advanced Purification Glass Columns for Protein Purifications

Waters series of glass columns are constructed of biocompatible glass

and polymeric materials and can be easily used with silica, polymer,

or soft gel packings. To optimize flow and ensure uniform sample

distribution onto the packed bed, each column incorporates a

distributor. A replaceable filter protects the packing from large

particulate contaminants.

Empty AP glass columns are available in a variety of sizes and utilize

the same design to ensure predictable methods transfer among them.

AP glass columns are compatible with both analytical and preparative

HPLC and FPLC systems.

Waters columns feature non-metallic construction and adjustable

bed height with easy-to-use coarse and fine adjustments. The AP

glass columns are available in a variety of dimensions.

Advanced Purification (AP) Glass Cloumn Accessories and Spare Parts

AP Minicolumn Accessories and Spare Parts

AP Column Accessories and Spare Parts

AP-2 Column Accessories and Spare Parts

Column Dimension

Bed Volumn

(mL)

Flow Rate (mL/min)

Pressure Rating (psi/MPa)

Part No.

5 x 50 mm 0.8-1.2 0-4 1500 psi/10 MPa WAT064-01

5 x 100 mm 1.8-2.2 0-4 1500 psi/10 MPa WAT064-02

10 x 100 mm 5-8 0-4 1500 psi/10 MPa WAT021901

10 x 200 mm 13-16 0-4 1500 psi/10 MPa WAT021902

10 x 300 mm 21-24 0-4 1500 psi/10 MPa WAT021903

10 x 600 mm 45-48 0-4 1500 psi/10 MPa WAT021906

20 x 100 mm 22-31 4-16 1000 psi/6.8 MPa WAT027501

20 x 200 mm 53-62 4-16 1000 psi/6.8 MPa WAT027502

20 x 300 mm 85-94 4-16 1000 psi/6.8 MPa WAT027503

20 x 600 mm 179-188 4-16 1000 psi/6.8 MPa WAT027506

50 x 100 mm 137-196 16-100 500 psi/3.4 MPa WAT023331

50 x 200 mm 333-392 16-100 500 psi/3.4 MPa WAT023332

50 x 300 mm 530-589 16-100 500 psi/3.4 MPa WAT023333

50 x 600 mm 1118-1177 16-100 500 psi/3.4 MPa WAT023336

Description Dimensions of Column Part No.

Glass Tube 5 x 50 mm WAT038802

5 x 100 mm WAT038803

Column Jacket 5 x 50 mm WAT038804

5 x 100 mm WAT038805

Filters, 10/pkg WAT038806

O-rings, 13/pkg(includes 10 inlet/outlet and 3 funnel)

WAT038807


Glass tube 10 x 100 mm WAT021992

10 x 200 mm WAT022033

10 x 300 mm WAT022034

10 x 600 mm WAT022035

Plastic shield 10 x 100 mm WAT021927

10 x 200 mm WAT021945

10 x 300 mm WAT021946

10 x 600 mm WAT021947

O-rings, 5/pkg WAT021907


Replacement tubing (Tefzel)1/16 inch o.d. x 0.009 inch i.d. x 10 feet(1.6 mm o.d. x 0.23 mm i.d. x 3 m)

WAT021950


Glass tube 20 x 100 mm WAT019891

20 x 200 mm WAT019892

20 x 300 mm WAT019893

Plastic shield 20 x 100 mm WAT027542

20 x 200 mm WAT027543

20 x 300 mm WAT027544

20 x 600 mm WAT027545



Replacement tubing (Tefzel)1/8 inch o.d. x 0.040 inch i.d. x 10 feet(3.2 mm o.d. x 1.02 mm i.d. x 3 m)

WAT023344


Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

AP-5 Column Accessories and Spare Parts


Glass Tube 50 x 100 mm WAT019876

50 x 200 mm WAT019877

50 x 300 mm WAT019878

Plastic Shield 50 x 100 mm WAT023370

50 x 200 mm WAT023371

50 x 300 mm WAT023372

50 x 600 mm WAT023373


Filter, 2/pkg WAT023343

Replacement tubing (Tefzel)1/8 inch o.d. x 0.040 inch i.d. x 10 feet)(3.2 mm o.d. x 1.02 mm i.d. x 3 m)

WAT023344

©2016 Waters Corporation. Waters and T he Science of What’s Possible are registered trademarks of Waters Corporation. AccellPlus and Protein-Pak are trademarks of Waters Corporation. Tefzel is a registered trademark of E.I. DuPont de Nemours and Company. Pharmacia and FPLC are registered trademarks of Pharmacia LKB. Ryton is a registered trademark of Phillips Chemical Company.

©2016 Waters Corporation. Produced in the U.S.A. July 2016 WAT021938 Rev 6 IH-PDF

Documents

WATERS ADVANCED PURIFICATION COLUMNS · reduce flow to 0 mL/min, Adjust the inlet connector until the void is eliminated. (If this is unsuccessful, see “Eliminating Voids”). 3