Supplementary data

Biodegradable nanotheranostics with hyperthermia-induced

bubble ability for ultrasound imaging guided chemo-

photothermal therapy

Changsong Xu1, 2, a Feng Gao3, a Jianrong Wu4, Shiwei Niu4, Fan Li3, Lifang Jin3, Qiusheng

Shi3, ⁎, Lianfang Du1, 3, ⁎

1 Department of Ultrasound, Shanghai General Hospital of Nanjing Medical University,

650 Xin Songjiang Road, Songjiang District, Shanghai 201600

2 Department of Ultrasound, The Affiliated Huaian No.1 People’s Hospital of Nanjing

Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Jiangsu,

China, 223300

3 Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin

Songjiang Road, Songjiang District, Shanghai 201600

4 College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,

Shanghai 201620, China

a These authors contributed equally.

Correspondence: Dr. Qiusheng Shi

Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin

Songjiang Road, Songjiang District, Shanghai 201600

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Tel: +86-13386259562

Fax: (0086) 021-37798276

E-mail: sqs19631989@163.com

Prof. Lianfang Du

Department of Ultrasound, Shanghai General Hospital of Nanjing Medical University, 650

Xin Songjiang Road, Songjiang District, Shanghai 201600

Tel: +86-13386259562

Fax: (0086) 021-37798276

Email: lianfang_du@126.com

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Figure S1. Energy-dispersive spectroscopy (EDS) line scanning of HMONs

nanoparticles.

Figure S2. Pore size distribution of HMONs, HMONs-PFP/DOX and PHDP

nanoparticles.

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Figure S3. Photographs of a) free PFP and PFP@HMON in PBS before b) and after

ultrasound sonication for 2 min c).

Figure S4. TGA curves of HMONs-PFP/DOX and PHDP nanoparticles.

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Figure S5. Hydrodynamic size of PHDP nanoparticles dispersed in water, PBS or

DMEM medium for seven days.

Figure S6. In vitro DOX release profles from HMONs-PFP/DOX at different pH

values.

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Figure S7. Uptake of Si element in MDA-MB-231 cells treated with the PHDP nanoparticles for 2 h, 4 h, and 6 h, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001.

Figure S8. Relative viability of MDA-MB-231 and A549 cells after incubation with

PDA@HMONs-PFP nanoparticles (concentrations of 50, 100, 150, 200, and 250 µg

mL−1) for 24 h.6

Figure S9. Time-dependent temperature changes in the MMDA-MB-231 tumor-

bearing nude mice after treated with saline and PHDP upon NIR laser irradiation for

10 min.

Figure S10. H&E stained histological images of major organs (heart, liver, spleen,

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lung, and kidney) from mice after different treatments. Scale bar: 100 μm.

Table S1. The parameters of HMONs, HMONs-PFP/DOX and PHDP nanoparticles.

Sample 　 Hydrodynamic diameters (nm) PDI Zeta potential (mV)HMONs 　 　 92.3 ± 5.6 0.243 -27.6 ± 2.6　 　 　 　 　 　 　 　HMONs-PFP/DOX 　 104.6 ± 9.4 0.212 -9.3 ± 2.1　 　 　 　 　 　 　 　PHDP 　 　 125.1 ± 11.6 0.134 -17.3 ± 1.5

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