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Name: __________________________________________ A & P Period: ______
Date: Title: Calcium & Bone Strength
Exp. Number4
Experiment/Subject:Calcium & Bone Strength
Date: Put date lab was performed
Name: YOUR NAME Lab PartnerWHO YOU WORKED WITH
Locker/Desk No. N/A
Course & Section #Class & Period
Question:How does removing calcium from bones with various liquids affect bone strength?
Background information (don’t copy into nb)CALCIUM: Calcium is an important mineral needed for multiple functions within the body. It is the most abundant mineral in the body, and is used so often that it must constantly be replenished through the diet. More than 99% of the calcium in your body is stored in the bones. The calcium found in bones helps maintain the strength and structure of bones, along with other minerals such as phosphorus, but it is also stored in bones for removal when the body needs it. Bones are composed of a variety of substances. Calcium compounds are found in the hard, outer layer of bones. Calcium provides for the toughness and strength of bones. Bone cells remove calcium phosphate and calcium carbonate from the blood to form bone in a process called ossification Specialized cells in bone tissue are responsible for adding and removing calcium from the bones depending on the amount of calcium in the bloodstream. This means only 1% of calcium is normally found in the body outside of bone and is crucial to important body functions
Calcium in the bloodstream and body tissues functions to: Allow cells to respond to cellular signals, such as hormones Stabilize the blood pressure in vessels Assist insulin in allowing glucose to enter cells Assist in blood clotting Assist in the release of neurotransmitters at the synapse of nerves Facilitate muscle contractions (contractions WOULD NOT occur without calcium) Assist sperm in the fertilization of the egg
Essentially calcium is needed for the heart to pump, the brain and nerves to work, to keep our bones hard, to make our muscles move, even for reproduction of the human species, and this is only a list of the most important functions!
Lab4
Osteoperosis:Calcium is continually added to bone from about the thir
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dtrimester of pregnancy into early adulthood. At this point, a smallamount of calci
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um will be lost from bones naturally, as bone-forming processes become slower
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than bone removal. Thisnatural bone loss is called osteopenia, and makes bon
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esslightly less dense and more prone to fracture. In someindividuals, the rate and am
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ount of mineral loss is acceleratedand can lead to a condition called osteoporosis,
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in which bones become very porous, fragile, and brittle. More than 80% of those
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who suffer from osteoporosis are women over the age of 65. More than 55% of peo
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ple over the age of 50 have some degree of osteoporosis.
Using a specialized
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x-ray machine, the density of bone minerals, such as calcium, can be determined
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through a bone mineral density (BMD) test. A BMD test can diagnose the onset of
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osteoporosis. There are treatment options that can decrease the risk of osteopor
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osis that include diet changes, exercise, and medications.
Case Scenario
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:You are a family physician at HASPI Medical Center. You have just informed a 4
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3-year-old female patient that her bone mineral density test shows early signs of oste
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oporosis. She is a pack-a-day smoker and does not really want to change any of
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her lifestyle habits, or take calcium supplements. The patient does not understan
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d why it is such a big deal that she is losing calcium, and thinks it is just a lie made
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up by the supplement companies. In order to create a visual example of the impact
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of calcium loss on bone tissue, you have set up the following experiment for your
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patient. Conduct the experiment over the next week to show your patient what ca
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n happen when important minerals and/or bone tissues are lost.
Introduction: Write this section AFTER you have completed the hypothesis and procedure.Make sure to include:
T = Topic (state goals & objectives of lab) I = Introduction of Evidence (what data will be collected) E = Evidence (how it will be collected—summarize experiment) D = Development of Evidence (what data might you expect and what conclusion can you come to) C= Conclusion
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variables:
Independent variable:
Dependent variable:
Constants:
hypothesis:*Write it using if-then-because statement. (Predict which liquid would affect [increase/decrease] the strength the most)
equations:N/A
materials:
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30 mL Vinegar (Acetic Acid)
Bleach30 mL Coke 30 mL isopropyl alcohol30 mL Water Triple Beam BalanceGraduated cylinder
Chicken bones3 Beakers/cupsPaper towels
Procedure:List each step and write it in the command voice.
Observations:1. Obtain three beakers/cups and label one with a “W” for
water, one with a “V” for vinegar, and the other with a “X” for liquid of choice
2. Using the graduated cylinder, measure out 30 ml of water and pour it into the beaker/cup labeled “W”.
3. Using the graduated cylinder, measure out 30 ml of vinegar and pour it into the beaker/cup labeled “V”.
4. Using the graduated cylinder, measure out 30 ml of chosen liquid and pour it into the beaker/cup labeled “X”.
5. Choose three chicken bones and remove all excess muscle from the bone. Dry off the bones with a paper towel. MAKE SURE TO WASH YOUR HANDS AFTER HANDLING RAW CHICKEN!!!
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Obtain a paper towel and scale. Weigh the paper to
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welWith a paper towel on the scale, weigh each chi
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cken bone separately. Subtract the weight of the t
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owel from the total weight w/ bone and record bone
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’s weight in as the starting weight for each colum
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n in the table.Place a bone in the beaker/cup cont
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aining water. Make sure the bone is completely cov
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ered. If it is not covered, then add more water.Pl
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ace a bone in the beaker/cup containing vinegar. M
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ake sure the bone is completely covered. If it is
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not covered, then add more vinegar.Place the third
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bone in the beaker/cup containing liquid of choic
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e. Make sure the bone is completely covered. If it
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is not covered, then add more bleach.Make your in
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itial observations of the bones. This could includ
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e the bones’ color, texture, smell, firmness, etc.
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Place all three beakers/cups in a safe place where
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they will not be disturbed. Use a piece of paper
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or paper towel with your group name placed under t
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he beakers/cups to identify them. The bones will b
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e observed and weighed at the end of five days.WAS
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H YOUR HANDS WITH SOAP AND WATER EVERY TIME YOU HA
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NDLE THE CHICKEN BONES!
After 5 daysProce
O
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dure:List each step and write it in the command voice
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.Remove the bone from the water beaker/cup and dry
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it off on a paper towel.Obtain a paper towel and s
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cale. Weigh the paper towelPlace the bone on the p
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aper towel and record its weight in grams. Subtrac
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t the weight of the towel from the total weight w/
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bone and record weight of bone in grams. Make a n
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ote of any visual observations as well.Repeat Step
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2 for the other bones.
Data and calculations:Weight of Paper Towel: _______________________________________________
Table 1. Bone Weight
TimePeriod
Water (Control) VinegarWeight of
paper towel + bone (g)
Weight of bone (g)
Weight of paper towel +
bone (g)
Weight of bone (g)
Weight of paper towel +
bone (g)
Weight of bone (g)
Initial(0 hr)
96 hours
Table 2. Bone Observations & Weight
TimePeriod
Water (Control) VinegarWeight
from Table 1
Observations Weight from
Table 1
Observations Weight from
Table 1
Observations
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GRAPH:Create a double bar graph of the weights for each bone before and after the test period on the graph provided below. Be sure your graph includes:
Title for the graph X and y axis clearly labeled (with titles and units) Bars for initial weight and post weight A key to distinguish the bars Number scale with evenly spaced intervals
Title: ________________________________________________________________
Key:
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discussion:
1. What liquid did you and your partners choose to soak chicken bone C in?
2. Why did you choose that particular liquid?
3. Compare and contrast the three bones placed in the various liquids.
4. Was there any evidence that calcium was removed from any of the bones? If so, what was it? From which liquids was the calcium removed?
5. Why do you think the liquids had the effect they did on the bones? What did those liquids have in common?
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conclusion: My hypothesis was correct incorrect inconclusive (circle one)
2 pieces of evidence (use numbers) that proved my hypothesis to be correct:
1.
2.
Sources of error:
1.
2.
Things to change in the experiment:
1.
2.
Two questions about experiment:
1.
2.
REAL WORLD CONNECTION: Explain how these results show the impact of calcium loss in bones. How does this experiment reflect what happens in osteoporosis?
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Group work reflection:Roles: (4 pts) Please
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put first AND last name!
1.T
2.S
3.D
4.R
Checklist: (4 pts)
D
D
W
W
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Answer the following questions: (10 pts)
What worked well the most
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during the assignment for the group?
What did not work so well during the ass
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ignment for the group? Where did you disagree as a group or saw mistakes in eac
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h other’s work?
What will you do next time to improve how your group worke
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d together?
How much did you as an individual participate in the group?
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What can you do next time to improve your own performance in the lab group
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?
Group Rubric:
Poor1 point
Developing2 points
Good3 points
Excellent4 points
Group Rating(YOU
RATE)
TeacherRating
ContributionOne or more members do
not contribute.
All members contribute, but
some contribute more than
others.
All members contribute equally.
All members contribute equally,
and some even contribute more
than was required.
CooperationTeacher
intervention needed often to
help group cooperate.
Members work well together some of the time. Some
teacher intervention
needed.
Members work well together
most of the time.
All members work well together all of
the time; assist others when
needed.
On taskTeam needs
frequent teacher
reminders to get on task.
Team is on task some of the time. Needs
teacher reminders.
Team is on task most of the time.
Does not need any teacher reminders.
Team is on task all of the time.
Does not need any teacher reminders.
Communication
Members need frequent teacher
intervention to listen to each
other and speak to each other appropriately.
Members need some teacher
intervention to be able to listen
to each other and speak to each other
appropriately.
All members listen to each
other and speak to each other in
equal amounts.
Each member listens well to
other members. Each member
speaks in friendly and encouraging
tones.
Total Score: /32 /16 /16
TOTAL SCORE ON REFLECTION: _________/50
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GROUP RATING: _________/32
Completion of Lab: ____________/50
Overall Score on Lab: _____________
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