Flow Cytometry for Analysis and Sorting of Large Objects using Union Biometrica’s

COPAS® and BioSorter® Instruments

Many objects are too large or fragile for conventional flow cytometry. Union Biometrica, produces two instrument platforms for large particle flow cytometry, specifically the COPAS and BioSorter. These instruments are designed for the automated analysis and sorting of large (10-1,500 micron) objects at rates of 10-500 objects/second. Using object size, optical density and fluorescence intensity as sorting criteria, selected objects can be dispensed in multi-well plates or bulk receptacles. A gentle pneumatic sorting mechanism avoids harming sensitive objects thereby making the instrument suitable for live biological materials or sensitive chemistries. This includes fragile large cells & cell clusters such as hepatocytes, adipocytes, cardiomyocytes, kidney tubule cells, embryoid bodies, pancreatic islets, tumor spheroids and other organotypic spheroids of differing origins. Other suitable samples are small model organisms such as all stages of C.elegans, Drosophila eggs, embryos and larvae, and Zebrafish eggs, embryos and the early larval stages. Finally, small plant seeds, like Arabidopsis, and plant pollen, as well as beads and microcarriers of various sizes and shapes are all acceptable samples for analysis and sorting. The instrument is designed to handle multiple fluorescence excitation and emission wavelengths. Axial profiles can be generated for analysis and sorting based on the localization of fluorescence.

Technology Seminar

Weon Bae, PhD Senior application scientist

Date: 19th Mar, 2012 Time: 16:30 to 17:30

Venue: Seminar room #2

All are Welcome!

Tel: (+852) 2986 4468 Fax: (+852) 2986 4297 Email: corefac@hku.hk

COPAS BioSorter