Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Influence of whisking-related activity in the motor cortex on sensory processing in the somatosensory cortex of rodents

Werner Kilb

Institut für Physiologie und Pathophysiologie

Universitätsmedizin Mainz

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Background

Gentet et al. (2012)Nat Neurosci:15:607-12

Voltage-sensitive dye

Whole-cell recording

Petersen (2007) Neuron 56:339

Diamond et al (2008) Nature Reviews 9:601

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

BackgroundVoltage-sensitive dye

Whole-cell recording

Petersen (2007) Neuron 56:339

Diamond et al (2008) Nature Reviews 9:601

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Background

Diamond et al (2008) Nature Reviews 9:601

Ferezou et al. (2007) Neuron 56:907

Mao et al. (2011) Neuron 72:111

Matyas et al. (2010) Science 330:1240

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Central question

Voltage-sensitive dye

Whole-cell recording

Petersen (2007) Neuron 56:339

wM1

Modified from Diamond et al (2008)

How does neuronal activity in the whisker-related motor cortex influence information processing in the somatosensory (barrel) cortex?

b) What is the Contextual control?

How does the somatosensory cortex process whisker-related informationin the context of

whisking-related activity in the motor cortex?

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Short overview on planed experiments

wM1

Modified from Diamond et al (2008)

1.) In-vivo recordings

Stimulatory context

- MEA-recordings (FP, MUA, single-units) (simultan. in S1 and wM1)

- inter- and transcolumnar information flow/processing

- repetitive spike patterns

Interference with signal processingSilencing (Lidocain) at different levels of information processingBlocking motor outputs (d-Tubocur./Botulinumtoxin)Optogenetic activation/silencing

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Short overview on planed experiments.

wM1

Modified from Diamond et al (2008)

1.) In-vivo recordings - MEA-recordings (FP, MUA, single-units) (simultan. in S1 and wM1)

- inter- and transcolumnar information flow/processing

- repetitive spike patterns

2.) In-vitro recordings - motosensori slices and cortical slab preperations

- influence of motorcortex activation on neuronal properties (Em, PSCs, APs, Resonance) in different neurons of the barrel field

- effect of serotonin on neurons in the somatosensory cortex

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Preliminary workSimultaneous recordings of sensorimotor interactions

FP-recording

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Preliminary workMethods to define/analyze information processing?

Werner Kilb Preparatory meeting SFB 07. Sept. 2012

Influence between motor and sensory cortex during whisking

Integration into the CRC „Contextual control“Direct link to two other projects investigating „active sensing“

- Torfi Sigurdsson „Neural Circuits Underlying Corollary Discharge in Mouse Auditory Cortex“

- Prof. Kössl „Context-dependence of cortical auditory time perception”

May serve to give conceptual support (e.g. unit-activity underlying oscillatory events) to the (human) study regarding “spectrotemporal dynamics of feedforward and feedback processing in sensorimotor transformations“ (Prof. Kell).

The analysis of our datasets will benefit from the expertise provided within this consortium applying for this CRC. In particular I plan collaborations with

Prof. Wibral (Model free measures of information flow, dynamic causal modeling)

Dr. G. Schneider (Information capacity of a single oscillatory cycle)