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What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

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Plasmid Structure

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Page 1: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000
Page 2: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

What is a plasmid?

Plasmids are small, circular DNA strands.

They typically encode only a few genes.

They are independent of the chromosome.

≈160,000 bp in bacteria

≈100,000,000 bp in eukaryotes

≈3,000 bp

Page 3: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Plasmid Structure

Page 4: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Reporter genes

Page 5: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

GFP Tagging

Page 6: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000
Page 7: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Recombinant Gene Expression

Media includes dissolved iron and Sulfuric acid

Page 8: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Acid Mine Drainage

Page 9: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Protein Product Purification

Page 10: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000
Page 11: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Antibiotic Selection

Page 12: What is a plasmid? Plasmids are small, circular DNA strands. They typically encode only a few genes. They are independent of the chromosome. 160,000

Recombinant DNA Responsibility

Sex in the Sink: Gene-Swapping Bacteria Are Making New Superbugs

-NBC News