What is lactose intolerance?

Inability to digest lactose!

Catalyzed reaction

Enzyme:Substrate (reactant):Products:

Lactose Intolerance lab In pairs, you will observe what happens

when you add lactase (enzyme) to regular milk and lactose-free milk.

You will have to figure out which milk is which!

Talk to the text Plant-Based Ice Cream Hits European

Markets

Science has given ice cream a makeover, expanding the number of options for people with lactose intolerance who cannot digest most dairy products.

LACTOSE INTOLERANC

E LAB

Review TTTT What was special about the ice cream

“Lupinesse”?

Lactase catalyzed reaction

Steps Have the partner on the left grab a

green tray.ON THE YELLOW SHEET Read the introduction and answer the

pre-lab questions to prepare for the lab. After you have answered all the

questions, follow the experimental procedures.

Glucose indicator test The glucose strips will tell you whether

glucose is present in the liquid. Dip the glucose strips in briefly so that

the end gets wet with the liquid. Do not leave it in the liquid.

Wait 30 seconds and then check your results (color change) against the key provided.

Record both the color (in words) and the glucose concentration (in numbers).

Some tips! The substances you will use are along the back counter.

Positive (+) control = glucose solution Negative (-) control = water A & B milk

Take your test tubes to the counter to get the substances. Use the markings on your pipet to make accurate

measurements. Pipet = the tool like a dropper

Use the marker to crush the tablet WHILE IT IS STILL IN THE PACKET. Be careful not to puncture the packet.

Regular milk and lactose-free milk look the same, so make sure to label your test tubes.

Record your results as you go along.