What’s New from Waters · 2015-06-11 · Newborn Screening Inherited metabolic disorders ©2015 Waters Corporation 77 ... MS/MS in Neonatal Screening Laboratories ... Kuwait, Taiwan,

©2015 Waters Corporation 1

[ WELCOME ]


Lance Nicolaysen Senior Director

Americas Mass Spectrometry Business

Waters Corporation, USA


MS-Related Job Opportunities

Field Marketing Manager, Health Sciences (LC/MS Clinical / Forensic / Toxicology)

MA

Mass Spectrometry Sales Specialist WA

Chemistry Sales Specialist NJ

Field Sales Account Manager NC

Field Service Engineer NJ, TX, MD, CA, PA, MD, VA, LA

Inside Instrument Sales Specialist MA

Inside Sales Specialist - Chemistry Consumables MA

Mass Spectrometry Clinical Specialist - West District CA

Mass Spectrometry Sales Specialist Brazil

North District Business Development Manager MA

Regional Service Manager CA

Senior MS Applications Chemist MA

SFC/SFE Field Service Engineer CA

SFC/SFE Sales Specialist USA

www.waters.com/careers


Gordon Kearney Senior Manager

Mass Spectrometry Product Management

Waters Corporation, UK


What’s New from Waters: 2015

Waters Users’ Meeting

63rd ASMS Conference, St. Louis, MO

30th May 2015


Set up theme

Innovation

– Performance

– Access


Informatics

Chemistries

Standards and

reagents

Separations science

Mass spectrometry


Oasis PRiME HLB

How can Oasis PRiME HLB simplify sample preparation?

How does it compare to other sample preparation products?

Sales Tools!

Questions?


GlycoWorks™ RapiFluor-MS™ N-Glycan Kit

Come Visit to Play:


ACQUITY UPLC Glycoprotein BEH Amide 300Å 1.7 µm


Nu

Gen

esis

LM

S


Progenesis QI v2.1 New features for ASMS 2015

More compound databases

– Greater choice for successful compound ID

– NIST LC-MS/MS libraries

ChemSpider results refinement

– Theoretical fragmentation

– Elemental composition filtering

– Increased confidence in results

Pathways analysis

– Placing discoveries in biological context

– IPA (Ingenuity / Qiagen)

Breakfast Meeting Wednesday

– Book your place now!


UN

IFI

Streamlined Instrument Controls

Seamless Workflows

Integrated Ion Mobility Tools Sophisticated Reporting

UNIFI: Taking Data to Knowledge

UNIFI 1.8


The Evolution of Ion Mobility MS


2006 Synapt HDMS TriWave, DriftScope

2007 MALDI Synapt

2009 Synapt G2 HDMS 2nd Gen TriWave & QuanTOF

2010 MALDI Synapt G2 HDMS HDI Software, ETD

2011 Synapt G2S HDMS StepWave

2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFITM), MS Compare, IntellistartTM

2003 First T-Wave IMS

2004 Prototype SYNAPT Q-IMS-Tof

2001 Linear Field IMS


Gate

MobilityRegion

StorageRegion Quadrupole

Mass Filter

ElectrosprayTip

Pusher

Detector

Reflectron

LensesCollision

Cell

SecondTransferRegion

Electric Field

FirstTransfer Region

Stacked RingIon Guide

Ions



2007 MALDI Synapt










2007 MALDI Synapt











The Challenge of Ion Mobility



2007 MALDI Synapt











2007 MALDI Synapt











2007 MALDI Synapt









CCS

Structure Separation

Sensitivity Specificity


Opportunity

Measure CCS of every ion in every

analysis Incorporate CCS into routine workflows



2007 MALDI Synapt








2015 Accessible Ion Mobility


Beyond Resolution



Dual Channel 7.2GHz (up-sampled) 10-bit ADC

QuanTof2 Detector

x 1

x 0.03

ADC High Gain Channel

ADC Low Gain Channel

Peak Processing

Peak processing

ADC Test signal

Apply calibration

Combine From MS

ADC Amplifier


Benefits of QuanTof2


DT

m/z

DT

m/z

DT

m/z

RT

Inte

nsity

High Selectivity (HS) MS/MS


Routine ion mobility

• Just press START

• Simply Acquire

• Get to the Answers

Seamlessly from sample to answers


Clarity cleaner mass spectra

Confidence valuable CCS information for

every ion

System Productivity with UNIFI analytical

workflows

Benefits of Routine Ion Mobility


518.2235 m/z

441.2758 m/z

289.1923 m/z

617.3076 m/z 236.6 Å2 Verapamil

Clarity

Unlimited Product ion

HDMSE



Vion measured CCS(N2) values

Reserpine - 609.28 m/z : 252 Å2 - 0.10 % RSD

LueEnk – 556.27 m/z : 230 Å2 - 0.15 % RSD

Terfenadine – 472.32 m/z : 230 Å2 - 0.12 % RSD

Verapamil – 455.29 m/z : 211 Å2 - 0.17 % RSD

Sulphadimethoxine – 311.08 m/z : 168 Å2 - 0.15 % RSD

Caffine – 195.08 m/z : 136 Å2 - 0.22 % RSD

Data from 400 injections over 33 hours

<2% time after time


-5

-4

-3

-2

-1

0

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2

3

4

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Ch

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Cle

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Cle

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Cle

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Cle

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osa

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% C

CS

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Axis Title

Synapt WIV ISP/Vion IMS Q ToF Comparison April 2015

Pesticide

WIV ISP Study: SYNAPT/Vion Comparison for Pesticides

Dr Severine Goscinny, Scientific Institute of Public Health, Belgium At Waters’ user meeting

Confidence


Confidence



153 Coeluting Components Resolved with Vion Mobility

System Productivity



Mike Wilson

30th May 2015

What if...



Sample

What is REIMS?

iKnife Hand-held sampling device

Diathermy Generator

Informatics System

REIMS Source

Rapid Evaporative Ionization Mass Spectrometry


How does it work?


How does it work?


Product Availability


Workflow

Acquire Import Analyze Identify Review

o Fast, direct ionization o No sample prep required o No chromatography required


Workflow


o Easily manage large datasets o Handle multiple replicates o Enhance data quality


Workflow


o Easy to use multivariate statistics o Differentiate sample groups o Highlight significant features


Workflow


Flexible compound search methods o Compatible with popular databases o

Confident assignments o


Workflow


Effective visualization tools o Integrate with other informatics o

Employ pathway analysis o


Applications – Food Research


Applications – Food Research


Applications – Health Sciences Research


Applications – Health Sciences Research

The REIMS Research System with iKnife Sampling is intended for research use only, and is not intended for

diagnostic or therapeutic use


Find out more

Waters Hospitality Suite

Renaissance Grand Hotel, Ballroom E

Mon – Wed, 8pm – 11pm


Find out more

Visit us at the Waters Hospitality Suite

Renaissance Grand Hotel, Ballroom E

Mon – Wed, 8pm – 11pm


Find out more

www.waters.com/REIMS



Ambient Ionisation Mass Spectrometry

Mike Morris

Waters Users’ Meeting ASMS St Louis

30th May 2015


Newborn Screening Inherited metabolic disorders


Acylcarnitine analysis Duke University

- RCOOH

-(CH3)3N

-C4H8

CID

Butylation

CH2 CH CH

RCOO H

COOH (CH3)3N

CH2 CH CH

RCOO H

COOC4H8H (CH3)3N

CH2 CH CH COOH [ ]+

(m/z 85)

Dave Millington

Don Chace

Charlie Roe


Amino Acids & Acylcarnitines

Neutral and acidic amino acids Neutral Loss 102Da

Acylcarnitines Precursors of m/z85

2-minute measurement

Up to 50 metabolic disorders


Adoption of MS/MS in the US

North Carolina, 1997

New England, 1998

California, 2004

New York, 2005

Texas, 2006

“It’ll never catch on, you know!”


MS/MS in Neonatal Screening Laboratories

In the US:

– All states have Tandem MS programs

– >80% use Waters instruments

In 2015, between 12,000,000 and 15,000,000 babies will be screened using

tandem mass spectrometry.

In the UK:

– Leeds, Manchester, Birmingham, Newcastle, Sheffield, North

London, South London, Glasgow, Portsmouth, Glasgow

In Europe:

– Belgium, Germany, Netherlands, Sweden, Hungary, Italy,

Norway, Switzerland, Russia, UK, Denmark

RoW:

– Australia, Saudi Arabia, Brazil, Argentina, China, Canada, Hong

Kong, Korea, Turkey, Kuwait, Taiwan, Japan, Costa Rica,

Malaysia, Thailand, Oman, Philippines, Vietnam.


REIMS and DESI Plenary Speakers

Addressing complex and critical food integrity issues

using the latest analytical technologies

Professor Chris Elliott,

Queen’s University Belfast

Ambient MS in clinical diagnostics – from microbiology to MS-guided surgery Professor Zoltan Takats, Imperial College London


[ THANKS ]

Documents

What’s New from Waters · 2015-06-11 · Newborn Screening Inherited metabolic disorders ©2015 Waters Corporation 77 ... MS/MS in Neonatal Screening Laboratories ... Kuwait, Taiwan,