New Brunswick™ CelliGen® BLU single-use bioreactor system

When Less Is More

The New Brunswick CelliGen BLU Single-Use, Stirred-Tank Bioreactor System combines the benefits of single-use vessel technology with the performance and scalability of a stirred-tank design.

The BioBLU® single-use vessel portfolio features three interchangeable pitched-blade, single-use vessels in 5, 14 and 50 L total volume capacities, as well as the first single-use vessel to feature the proprietary Eppendorf packed-bed impeller. Add to that the intuitive Eppendorf RPC controller and you have the ideal system for high density cell culture processes from research through production.

Benefits > Eliminates vessel cleaning and autoclaving > Rapid turnaround, reduced validation and lower start-up costs > Advanced process control for all vessel sizes from a single controller > Proven performance and scalability of stirred-tank design > Unique pH and DO technology makes probe insertion totally noninvasive and autoclaving unnecessary > USP Class VI and animal component free material, making vessels appropriate for GMP production

Proven Cell Culture Technology Meets Single-Use Design

Ready for use right out of the box!

Pre-sterilized/pre-assembled vessels include: > Pitched-blade impeller with upflow or downflow rotation selectable via touchscreen > Choice of microsparge or macrosparge > Optical pH sensor > Tubing (silicone and C-Flex®, for use with tube welders) > 0.2 μm filters > Sealed magnetic drive with fully enclosed bearings > 5 L Packed-Bed vessel includes 150 g Fibra-Cel® solid support growth material

Vessel ports and penetrations include: > Threaded PG 13.5 port for auxilliary sensor > RTD/temperature > Noninvasive optical pH > Noninvasive polarographic DO > Additions (3) > Harvest > Overlay > Sparge > Sampling > Exhaust line with heater

BioBLU 14c

BioBLU 5c BioBLU 5p (packed bed)

BioBLU 50c

High-tech, small package > Sophisticated RPC (Reactor Process Control) software controls up to 32 process parameters, trends up to 8 loops, and provides built-in security > Large 38 cm (15 in) color touchscreen interface > 3 Thermal Mass Flow Controllers (TMFCs) standard — High and Low Flow options available > Integrated control of up to 8 gases for sparge and gas overlay > 3 built-in, high-performance, assignable pumps > Automated control of temperature, pH, DO and agitation > 3- or 4-gas auto mix function simplifies process control > Monitor pH through optical and traditional (gel-filled) probes > Vessel heat blanket provides uniform temperature > Exhaust line heat blanket prevents filter clogging > 7 analog inputs, 7 analog outputs for ancillary devices

Innovative pH sensor and proprietary DO probe sleeve > Eliminate contamination risk—no direct contact between probes and vessel contents > Save hours of prep time and labor—no autoclaving or cleaning is needed; the reusable DO probe can stay attached to the control station, polarized and ready for use > Enable vessel setup right on the bench—no biosafety cabinet/hood needed for operation > No autoclaving extends DO probe life

A wide range of optional equipment > Scale for measuring vessel volume or feeds > 4-gas overlay with choice of TMFC or Rotameter > Validation packages (leachables/extractables reports) > Gas regulators > Media/supplement addition kits > BioCommand® SCADA software

C-Flex® is a registered trademark of Saint-Gobain Plastics. Eppendorf®, the Eppendorf logo and BioBLU® are registered trademarks of Eppendorf AG, Germany. New Brunswick™ is a trademark of Eppendorf AG, Germany. BioCommand® and CelliGen® are registered trademarks of New Brunswick Scientific Co., Inc., USA. Fibra-Cel® is a registered trademark owned by Imerys Minerals California, Inc., USA and licensed to New Brunswick Scientific Co., Inc., USA. All rights reserved, including graphics and photos. Order no B-3009a. Copyright © 2013 by Eppendorf AG.

CelliGen® BLU system specifications

Controller

Design/function Controls up to 32 control loops; stores 10 recipes and 8 process variables for trend graphing; includes an industrial touchscreen monitor/user interface, 3 built-in pumps and connectors for all utilities and communications signals

Display 15-inch (38.1 cm) color industrial touchscreen interface with adjustable-angle swivel

Temperature

Range From 5° C above ambient to 40° C

Control PI for heating and cooling: Heating via Silicone Heat Blanket and Cooling via ambient tempe-rature

Sensor type Pt 100 platinum RTD probe

Aeration

Gas mix Up to 8 gases, including air, N2, CO2 and O2, delivered to sparge and overlay

Sparge flow control 3 TMFC only (N2 and O2 share TMFC) High-Flow option (0.04 - 7.5 SLPM) Low-Flow option (0.002 - 1.0 SLPM)

Sparge elements Porous Microsparge (7 - 12 μm) Drilled Macrosparge (0.7 mm)

Overlay flow control 1 TMFC (0.05 - 5.00 SLPM) Automatic option1 Rotameter (0.2 - 5.0 SLPM) Manual option

Inlet filters 0.2 μm disposable absolute filter

pH

Range 6 - 8 pH (optical), 2 - 14 (gel-filled)

Control PI-controlled

Sensor type Noninvasive optical pH probe. Single-use fluorescence sensor included with each vessel. Gel-filled probe optional.

Exhaust Filter 0.2 μm disposable absolute filter (heat-blanketed to reduce clogging)

DO

Range/control 0 - 200 % • PI-controlled

Sensor type Noninvasive, reusable polarographic probe

Pumps

Pump 1 (14 rpm) Assignable peristaltic pump; fixed speed or variable duty cycle; available control modes: Off, On, Prime, Acid, Harvest, Add

Pumps 2 & 3 (109 rpm) Assignable peristaltic pumps; fixed speed or variable duty cycle; available control modes: Off, On, Prime, Acid, Harvest, Add

Electrical 100 - 120 VAC: 50/60 Hz, Single phase, 15 Amps 200 - 240 VAC: 50/60 Hz, Single phase, 15 Amps

Gas Clean Dry Gasses regulated to 6 PSIG (0.41 Bar) maximum

Net Weight

Control station 40 kg (88 lbs.) with touchscreen

Touchscreen 6.8 kg (15 lbs.)

Motor 4.8 kg (10.6 lbs.)

Dimensions, Control Station and Vessel

63 x 61 x 86 cm (25 x 24 x 34 in.), including touchscreen (W x D x H)

External Connections

Analog inputs/outputs

7 inputs and 7 outputs to connect auxiliary equipment:• 3 inputs and 3 outputs —

interchangeable 4 - 20 mA or 0 - 5 V• 4 inputs and 4 outputs — 0 - 5 V

2 USB ports Import software updates, export trend data, connect to scale interface box

RS-485 comm. port For optional BioCommand®/SCADA software

Ambient OperatingConditions

15 - 30° C, up to 80 % relative humidity, non-condensing

Regulatory Compliance

CAN/CSA-C22.2 Nos. 1010.1 &1010.2.010UL Standard UL-61010A-1 & 61010A-2-010

Single-use cell culture vessels specifications

Vessel BioBLU® 50c BioBLU® 14c BioBLU® 5c BioBLU® 5p (Packed-Bed)

Max. working volume 40 L 10.5 L 3.75 L 3.75 L

Min. working volume 18 L 3.5 L 1.25 L 3.75 L

Height : Diameter ratio 1.7 : 1 2 :1 1.5 : 1 —

Impeller diameter 160 mm 100 mm 100 mm —

Agitation

Range 25 - 150 rpm 25 - 200 rpm 25 - 200 rpm 25 - 200 rpm

Control / sensor type / impellers PI-controlled • Optical encoder • Pitched blade (upflow or downflow selectable via touchscreen)

Power number (nP) 1.3 1.3 1.3 —

Drive Permanent magnet motor with high torque input

Net weight, empty vessel 4.5 kg (9.9 lbs.) 1.8 kg (3.9 lbs.) 1.3 kg (2.9 lbs.) 1.5 kg (3.3 lbs.)

www.eppendorf.com

Your local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hamburg · Germanynewbrunswick@eppendorf.com