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Image Cytometry System for 20 µl Cell-Based Assays
Cellometer
® Spectrum
Nexcelom Bioscience360 Merrimack Street, Building 9Lawrence, MA 01843, USA
Nexcelom products are for RESEARCH USE ONLY and are not approved for diagnostic or therapeutic use. © Copyright 2018 Nexcelom Bioscience LLC. All Rights Reserved.
Innovation and Expertise in the Science of Cell CountingSchedule a FREE demonstration or technical seminar with a Nexcelom Applications Specialist today.
Call 978-327-5340 or E-mail [email protected]
1001465 Rev B 7/18
For Europe Call 44 (0) 161 232 4593 orE-mail [email protected]
For China Call +86 21 5886 0038 orE-mail [email protected]
Cell CycleApoptosisCell Mediated CytotoxicityCell Counting and ViabilityInterchangeable Fluorescent Modules
Accurate Cell Counting
R2=0.99899
1.00E+04
1.00E+05
1.00E+06
1.00E+07
1.00E+06 1.00E+07 1.00E+08
Theo
retic
al C
once
ntra
tion
[log]
Measured Concentration [log]Counted Cells Trend Line
Broad Dynamic Range1.00E+08
1.00E+051.00E+04
• AutomaticidentificationofAcridineOrange(AO)stainedcells(shownabove)
• Image and count up to 2x107 cells/mL
• Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
• Versatile platform - use for routine cell countingandcomplexcell-basedassays
Bright Field StainedAO+Cells CountedAO+Cells
Cell Imaging• Visually check cell morphology
• Ensure only cells of interest are counted
• Archiveandre-analyzecellimages
• Exportimagesforpublication
Proprietary Pattern-Recognition Software• Countindividualcellsinclusters
• Count irregular-shaped cells
• Countcellsbasedonsize
• Eliminatedebrisfromcellcounts
Non-Fluidic Platform• Disposablecountingchambers-nowashing
• Compatiblewithfragilecells
• Maintenance-free
• RobustopticsmodulesandLEDlightsources
IQ/OQ Validation and GMP/GLP Accessories• InstallationQualificationreagents/protocol
• OperationalQualificationreagents/protocol
• On-siteIQorOQPerformance
• GMP/GLPSoftwareModule
Which Instrument is Right for Me?
Features Bright Field Cell Counters Fluorescent Viability Cell Counters Image Cytometers
Mini Auto T4 Auto 1000
Auto 2000 X1 X2 K2 Spectrum Spectrum
(10x)Celigo
BFCeligo 4 Channel
Celigo 5 Channel
Cell / Sample TypeCell Line X X X X X X X X X
CulturedPrimaryCells X X X X X X X X XAlgae X
Platelets X XLow Concentration Cell Lines X X X X X X
Yeast (Clean Sample) X X XYeast (Messy Sample) X X
Primarycells(MessySample*) X X X X XPBMCs,Splenocytes,StemCells X X X X X
Hepatocytes X X X XAdipocytes*** X X X X X X
Cell-BasedAssay** X X X X X X X XApoptosis(AnnexinV-FITC/PI) X X X X XApoptosis(CaspaseActivity) X X X X X
Autophagy (CytoID-green) X XCellProliferation(CFSE) X X X X
CellCycle(PI) X X X X X X XGFPTransfection X X X X X X XRFPTransfection X X X X
MitochondrialPotential(JC-1) X X X XMulti-drug Resistance (ABC
Transporter) X X X X
Surface Marker Analysis X X X XVitality(Calcein-AM/PI) X X X X X X
Vitality (CFDA-AM) X XImage Cytometry** X X X X X
* A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.** FCS Express license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter
Advantages of Cellometer Image Cytometry
Cell Types for Many Research Areas
• Clinical Immunology: PBMCs
• Diabetes / Obesity: Adipocytes
• Immunotherapy: Leukocytes
• Microbiology: Yeast (Spectrum 10x)
• Oncology: Cell Lines
• Regenerative Medicine: Stem Cells
• Toxicology: Hepatocytes
• Transplantation: Nucleated Cells
• Vaccine Development: Splenocytes
All-in-One System Basiccellcounting,primarycellviability,andcell-basedassays.
Dual-Fluorescence for Accurate Primary Cell Viability No interference from red blood cells.Analyzebonemarrow,peripheralblood,andcordbloodwithoutlysing.
Unique Algorithms for Advanced Cell AnalysisDetermine concentration and viabilityof hepatocytes, adipocytes, and othersophisticated cell types.
Fast ResultsObtain cell images, counts, sizemeasurementsandviabilitycalculationsin < 30 seconds per sample.
Features of the SpectrumTM Image Cytometry System
Optimizedforprimarycellanalysis
PBMCsStem CellsAdipocytesNeural CellsHepatocytesDendritic Cells
Epithelial CellsKeratinocytesLymphocytesSplenocytesMonocytes
Simple 20 µl Cell-Based Assays• Pre-qualifiedreagents
• Small20µlsamplesize
• Simple,image-basedanalysis
• Pre-definedinstrumentsettings
• Assay-specificdatatemplates
• Accurate,consistentresults
1.Pipette20µl 2. Insert slide & count
3. Get images & data
Cell Population
% of Gated Cells
Concentration (106 cells/mL)
Total 100 3.18
SubG1 3.8 0.12
G0/G1 61.9 1.97
S 15.3 0.49
G2/M 19 0.60
Population gate: 94.7% of total cells countedNumber of gated cells: 4475 cellsConcentration of gated cells: 3.18 x10^6 cells/mL
Assay Infor ation
Assay Name: FCS_Cell Cycle-PIDescription:Date of experiment: 06/15/2018 10:55:09Instrument: Spectrum-101-0002Optics Module 1: S1-655-527 Exposure Time: 300.0 msec
CC4_8 images.NXDat
0 7500 15000 22500 300000
6.25
12.50
18.75
25.00
Population
Export to FCS ExpressTM* for Flow-Like Data
• Exportimagedataintoflowcytometrysoftware: FCS ExpressTM
• Simpleworkflow:Nofluid-stream,NoPMTvoltages,Noforward/sidescatter
• Easily perform data analysis using pre-designed templates
• Quickly plot cell population data as a: histogram,scatter,dotorcontourplot
Size(m
icron)
FL 1 (intensity)
* FCS ExpressTM Flow Cytometry software is a product of De Novo SoftwareTM and is included with the Cellometer SpectrumTM
CountedPI+Cells ContourPopulationPlot
User-Changeable Fluorescence Optics Modules*
*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
Cellometerassaysusespecificopticmodulesformaximumperformanceanddiscrimination between fluorescence channels. Example filtermodules arelistedbelow.Additionalfiltermodulesavailableuponrequest.Pleasecheckourwebsiteforthemostrecentmodulesavailable.
Optics Module Fluorophores Nucleic Acid Stains
S1-534-470Ex: 470nm (452-498nm)Em: 534nm (510-560nm)
GFPCalceinFITCAlexaFluor® 488
AO(acridineorange)SYTO®9,SYTO®13SYTOX®GreenSYTO®BC
S1-605-527Ex: 527nm (498-550nm)Em: 605nm (570-638nm)
AlexaFluor® 546AlexaFluor® 555Cy3®
PE(R-phycoerythrin)RFP
PI(propidiumiodide)EB(ethidiumbromide)SYTOX®Orange
S1-655-527Ex: 527nm (525-558nm)Em: 655nm (642-665nm)
RFP7-AADNile Red
PI(propidiumiodide)EB(ethidiumbromide)
R2=0.99498
0.00%10.00%20.00%30.00%40.00%50.00%60.00%70.00%80.00%90.00%
100.00%
0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
Expe
cted
% V
iabi
lity
Measured % Viability
Measured Jurkat Cell Viability
Proven Results
Bright Field StainedAO+Cells
StainedPICells CombinedCountedCells
PI (intensity)
Cou
nts
0 7000 14000 21000 28000 350000
17
34
52
69
86
Legend Sub G1 G0/G1 S G2/M
Propidium Iodide (PI) Cell Cycle
CD3 Bright Field CD3PE
FL1 ( int ensit y )
Coun
t
0 101 1020
12
23
35
46
CD3+90.14%
Bright Field GFP/RFPPositiveCells
GFP/RFP Population Analysis
• Determinecellviability,forcelllinesorprimarysamples,usingAO/PIinseconds
• Accuratelymeasurecellsampleswithvaryingviability (0 - 100%)
Measured Jurkat Cell Viability
Histogram of PE CD3+ Jurkat cells
Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.
Accurately count Live (AO) versus Dead (PI) cells.
RFPsity)
FL1 (intensity)
FL2
(inte
nsity
)
101
102
103
104
101
102
103
104
17.57%45.00%
2.72%34.60% Top Left: RFP+Top Right: RFP+ and GFP+Bottom Right: GFP+Bottom Left: FL-
Image Cytometry System for 20 µl Cell-Based Assays
Cellometer
® Spectrum
Nexcelom Bioscience360 Merrimack Street, Building 9Lawrence, MA 01843, USA
Nexcelom products are for RESEARCH USE ONLY and are not approved for diagnostic or therapeutic use. © Copyright 2018 Nexcelom Bioscience LLC. All Rights Reserved.
Innovation and Expertise in the Science of Cell CountingSchedule a FREE demonstration or technical seminar with a Nexcelom Applications Specialist today.
Call 978-327-5340 or E-mail [email protected]
1001465 Rev B 7/18
For Europe Call 44 (0) 161 232 4593 orE-mail [email protected]
For China Call +86 21 5886 0038 orE-mail [email protected]
Cell CycleApoptosisCell Mediated CytotoxicityCell Counting and ViabilityInterchangeable Fluorescent Modules
Accurate Cell Counting
R2=0.99899
1.00E+04
1.00E+05
1.00E+06
1.00E+07
1.00E+06 1.00E+07 1.00E+08
Theo
retic
al C
once
ntra
tion
[log]
Measured Concentration [log]Counted Cells Trend Line
Broad Dynamic Range1.00E+08
1.00E+051.00E+04
• AutomaticidentificationofAcridineOrange(AO)stainedcells(shownabove)
• Image and count up to 2x107 cells/mL
• Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
• Versatile platform - use for routine cell countingandcomplexcell-basedassays
Bright Field StainedAO+Cells CountedAO+Cells
Cell Imaging• Visually check cell morphology
• Ensure only cells of interest are counted
• Archiveandre-analyzecellimages
• Exportimagesforpublication
Proprietary Pattern-Recognition Software• Countindividualcellsinclusters
• Count irregular-shaped cells
• Countcellsbasedonsize
• Eliminatedebrisfromcellcounts
Non-Fluidic Platform• Disposablecountingchambers-nowashing
• Compatiblewithfragilecells
• Maintenance-free
• RobustopticsmodulesandLEDlightsources
IQ/OQ Validation and GMP/GLP Accessories• InstallationQualificationreagents/protocol
• OperationalQualificationreagents/protocol
• On-siteIQorOQPerformance
• GMP/GLPSoftwareModule
Which Instrument is Right for Me?
Features Bright Field Cell Counters Fluorescent Viability Cell Counters Image Cytometers
Mini Auto T4 Auto 1000
Auto 2000 X1 X2 K2 Spectrum Spectrum
(10x)Celigo
BFCeligo 4 Channel
Celigo 5 Channel
Cell / Sample TypeCell Line X X X X X X X X X
CulturedPrimaryCells X X X X X X X X XAlgae X
Platelets X XLow Concentration Cell Lines X X X X X X
Yeast (Clean Sample) X X XYeast (Messy Sample) X X
Primarycells(MessySample*) X X X X XPBMCs,Splenocytes,StemCells X X X X X
Hepatocytes X X X XAdipocytes*** X X X X X X
Cell-BasedAssay** X X X X X X X XApoptosis(AnnexinV-FITC/PI) X X X X XApoptosis(CaspaseActivity) X X X X X
Autophagy (CytoID-green) X XCellProliferation(CFSE) X X X X
CellCycle(PI) X X X X X X XGFPTransfection X X X X X X XRFPTransfection X X X X
MitochondrialPotential(JC-1) X X X XMulti-drug Resistance (ABC
Transporter) X X X X
Surface Marker Analysis X X X XVitality(Calcein-AM/PI) X X X X X X
Vitality (CFDA-AM) X XImage Cytometry** X X X X X
* A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.** FCS Express license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter
Advantages of Cellometer Image Cytometry
Cell Types for Many Research Areas
• Clinical Immunology: PBMCs
• Diabetes / Obesity: Adipocytes
• Immunotherapy: Leukocytes
• Microbiology: Yeast (Spectrum 10x)
• Oncology: Cell Lines
• Regenerative Medicine: Stem Cells
• Toxicology: Hepatocytes
• Transplantation: Nucleated Cells
• Vaccine Development: Splenocytes
All-in-One System Basiccellcounting,primarycellviability,andcell-basedassays.
Dual-Fluorescence for Accurate Primary Cell Viability No interference from red blood cells.Analyzebonemarrow,peripheralblood,andcordbloodwithoutlysing.
Unique Algorithms for Advanced Cell AnalysisDetermine concentration and viabilityof hepatocytes, adipocytes, and othersophisticated cell types.
Fast ResultsObtain cell images, counts, sizemeasurementsandviabilitycalculationsin < 30 seconds per sample.
Features of the SpectrumTM Image Cytometry System
Optimizedforprimarycellanalysis
PBMCsStem CellsAdipocytesNeural CellsHepatocytesDendritic Cells
Epithelial CellsKeratinocytesLymphocytesSplenocytesMonocytes
Simple 20 µl Cell-Based Assays• Pre-qualifiedreagents
• Small20µlsamplesize
• Simple,image-basedanalysis
• Pre-definedinstrumentsettings
• Assay-specificdatatemplates
• Accurate,consistentresults
1.Pipette20µl 2. Insert slide & count
3. Get images & data
Cell Population
% of Gated Cells
Concentration (106 cells/mL)
Total 100 3.18
SubG1 3.8 0.12
G0/G1 61.9 1.97
S 15.3 0.49
G2/M 19 0.60
Population gate: 94.7% of total cells countedNumber of gated cells: 4475 cellsConcentration of gated cells: 3.18 x10^6 cells/mL
Assay Infor ation
Assay Name: FCS_Cell Cycle-PIDescription:Date of experiment: 06/15/2018 10:55:09Instrument: Spectrum-101-0002Optics Module 1: S1-655-527 Exposure Time: 300.0 msec
CC4_8 images.NXDat
0 7500 15000 22500 300000
6.25
12.50
18.75
25.00
Population
Export to FCS ExpressTM* for Flow-Like Data
• Exportimagedataintoflowcytometrysoftware: FCS ExpressTM
• Simpleworkflow:Nofluid-stream,NoPMTvoltages,Noforward/sidescatter
• Easily perform data analysis using pre-designed templates
• Quickly plot cell population data as a: histogram,scatter,dotorcontourplot
Size(m
icron)
FL 1 (intensity)
* FCS ExpressTM Flow Cytometry software is a product of De Novo SoftwareTM and is included with the Cellometer SpectrumTM
CountedPI+Cells ContourPopulationPlot
User-Changeable Fluorescence Optics Modules*
*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
Cellometerassaysusespecificopticmodulesformaximumperformanceanddiscrimination between fluorescence channels. Example filtermodules arelistedbelow.Additionalfiltermodulesavailableuponrequest.Pleasecheckourwebsiteforthemostrecentmodulesavailable.
Optics Module Fluorophores Nucleic Acid Stains
S1-534-470Ex: 470nm (452-498nm)Em: 534nm (510-560nm)
GFPCalceinFITCAlexaFluor® 488
AO(acridineorange)SYTO®9,SYTO®13SYTOX®GreenSYTO®BC
S1-605-527Ex: 527nm (498-550nm)Em: 605nm (570-638nm)
AlexaFluor® 546AlexaFluor® 555Cy3®
PE(R-phycoerythrin)RFP
PI(propidiumiodide)EB(ethidiumbromide)SYTOX®Orange
S1-655-527Ex: 527nm (525-558nm)Em: 655nm (642-665nm)
RFP7-AADNile Red
PI(propidiumiodide)EB(ethidiumbromide)
R2=0.99498
0.00%10.00%20.00%30.00%40.00%50.00%60.00%70.00%80.00%90.00%
100.00%
0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
Expe
cted
% V
iabi
lity
Measured % Viability
Measured Jurkat Cell Viability
Proven Results
Bright Field StainedAO+Cells
StainedPICells CombinedCountedCells
PI (intensity)
Cou
nts
0 7000 14000 21000 28000 350000
17
34
52
69
86
Legend Sub G1 G0/G1 S G2/M
Propidium Iodide (PI) Cell Cycle
CD3 Bright Field CD3PE
FL1 ( int ensit y )
Coun
t
0 101 1020
12
23
35
46
CD3+90.14%
Bright Field GFP/RFPPositiveCells
GFP/RFP Population Analysis
• Determinecellviability,forcelllinesorprimarysamples,usingAO/PIinseconds
• Accuratelymeasurecellsampleswithvaryingviability (0 - 100%)
Measured Jurkat Cell Viability
Histogram of PE CD3+ Jurkat cells
Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.
Accurately count Live (AO) versus Dead (PI) cells.
RFPsity)
FL1 (intensity)
FL2
(inte
nsity
)
101
102
103
104
101
102
103
104
17.57%45.00%
2. 72%34.60% Top Left: RFP+Top Right: RFP+ and GFP+Bottom Right: GFP+Bottom Left: FL-
Cell Types for Many Research Areas
• Clinical Immunology: PBMCs
• Diabetes / Obesity: Adipocytes
• Immunotherapy: Leukocytes
• Microbiology: Yeast (Spectrum 10x)
• Oncology: Cell Lines
• Regenerative Medicine: Stem Cells
• Toxicology: Hepatocytes
• Transplantation: Nucleated Cells
• Vaccine Development: Splenocytes
All-in-One System Basiccellcounting,primarycellviability,andcell-basedassays.
Dual-Fluorescence for Accurate Primary Cell Viability No interference from red blood cells.Analyzebonemarrow,peripheralblood,andcordbloodwithoutlysing.
Unique Algorithms for Advanced Cell AnalysisDetermine concentration and viabilityof hepatocytes, adipocytes, and othersophisticated cell types.
Fast ResultsObtain cell images, counts, sizemeasurementsandviabilitycalculationsin < 30 seconds per sample.
Features of the SpectrumTM Image Cytometry System
Optimizedforprimarycellanalysis
PBMCsStem CellsAdipocytesNeural CellsHepatocytesDendritic Cells
Epithelial CellsKeratinocytesLymphocytesSplenocytesMonocytes
Simple 20 µl Cell-Based Assays• Pre-qualifiedreagents
• Small20µlsamplesize
• Simple,image-basedanalysis
• Pre-definedinstrumentsettings
• Assay-specificdatatemplates
• Accurate,consistentresults
1.Pipette20µl 2. Insert slide & count
3. Get images & data
Cell Population
% of Gated Cells
Concentration (106 cells/mL)
Total 100 3.18
SubG1 3.8 0.12
G0/G1 61.9 1.97
S 15.3 0.49
G2/M 19 0.60
Population gate: 94.7% of total cells countedNumber of gated cells: 4475 cellsConcentration of gated cells: 3.18 x10^6 cells/mL
Assay Infor ation
Assay Name: FCS_Cell Cycle-PIDescription:Date of experiment: 06/15/2018 10:55:09Instrument: Spectrum-101-0002Optics Module 1: S1-655-527 Exposure Time: 300.0 msec
CC4_8 images.NXDat
0 7500 15000 22500 300000
6.25
12.50
18.75
25.00
Population
Export to FCS ExpressTM* for Flow-Like Data
• Exportimagedataintoflowcytometrysoftware: FCS ExpressTM
• Simpleworkflow:Nofluid-stream,NoPMTvoltages,Noforward/sidescatter
• Easily perform data analysis using pre-designed templates
• Quickly plot cell population data as a: histogram,scatter,dotorcontourplot
Size(m
icron)
FL 1 (intensity)
* FCS ExpressTM Flow Cytometry software is a product of De Novo SoftwareTM and is included with the Cellometer SpectrumTM
CountedPI+Cells ContourPopulationPlot
User-Changeable Fluorescence Optics Modules*
*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.
Cellometerassaysusespecificopticmodulesformaximumperformanceanddiscrimination between fluorescence channels. Example filtermodules arelistedbelow.Additionalfiltermodulesavailableuponrequest.Pleasecheckourwebsiteforthemostrecentmodulesavailable.
Optics Module Fluorophores Nucleic Acid Stains
S1-534-470Ex: 470nm (452-498nm)Em: 534nm (510-560nm)
GFPCalceinFITCAlexaFluor® 488
AO(acridineorange)SYTO®9,SYTO®13SYTOX®GreenSYTO®BC
S1-605-527Ex: 527nm (498-550nm)Em: 605nm (570-638nm)
AlexaFluor® 546AlexaFluor® 555Cy3®
PE(R-phycoerythrin)RFP
PI(propidiumiodide)EB(ethidiumbromide)SYTOX®Orange
S1-655-527Ex: 527nm (525-558nm)Em: 655nm (642-665nm)
RFP7-AADNile Red
PI(propidiumiodide)EB(ethidiumbromide)
R2=0.99498
0.00%10.00%20.00%30.00%40.00%50.00%60.00%70.00%80.00%90.00%
100.00%
0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%
Expe
cted
% V
iabi
lity
Measured % Viability
Measured Jurkat Cell Viability
Proven Results
Bright Field StainedAO+Cells
StainedPICells CombinedCountedCells
PI (intensity)
Cou
nts
0 7000 14000 21000 28000 350000
17
34
52
69
86
Legend Sub G1 G0/G1 S G2/M
Propidium Iodide (PI) Cell Cycle
CD3 Bright Field CD3PE
FL1 ( int ensit y )
Coun
t
0 101 1020
12
23
35
46
CD3+90.14%
Bright Field GFP/RFPPositiveCells
GFP/RFP Population Analysis
• Determinecellviability,forcelllinesorprimarysamples,usingAO/PIinseconds
• Accuratelymeasurecellsampleswithvaryingviability (0 - 100%)
Measured Jurkat Cell Viability
Histogram of PE CD3+ Jurkat cells
Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.
Accurately count Live (AO) versus Dead (PI) cells.
RFPsity)
FL1 (intensity)
FL2
(inte
nsity
)
101
102
103
104
101
102
103
104
17.57%45.00%
2. 72%34.60% Top Left: RFP+Top Right: RFP+ and GFP+Bottom Right: GFP+Bottom Left: FL-
Image Cytometry System for 20 µl Cell-Based Assays
Cellometer
® Spectrum
Nexcelom Bioscience360 Merrimack Street, Building 9Lawrence, MA 01843, USA
Nexcelom products are for RESEARCH USE ONLY and are not approved for diagnostic or therapeutic use. © Copyright 2018 Nexcelom Bioscience LLC. All Rights Reserved.
Innovation and Expertise in the Science of Cell CountingSchedule a FREE demonstration or technical seminar with a Nexcelom Applications Specialist today.
Call 978-327-5340 or E-mail [email protected]
1001465 Rev B 7/18
For Europe Call 44 (0) 161 232 4593 orE-mail [email protected]
For China Call +86 21 5886 0038 orE-mail [email protected]
Cell CycleApoptosisCell Mediated CytotoxicityCell Counting and ViabilityInterchangeable Fluorescent Modules
Accurate Cell Counting
R2=0.99899
1.00E+04
1.00E+05
1.00E+06
1.00E+07
1.00E+06 1.00E+07 1.00E+08
Theo
retic
al C
once
ntra
tion
[log]
Measured Concentration [log]Counted Cells Trend Line
Broad Dynamic Range1.00E+08
1.00E+051.00E+04
• AutomaticidentificationofAcridineOrange(AO)stainedcells(shownabove)
• Image and count up to 2x107 cells/mL
• Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
• Versatile platform - use for routine cell countingandcomplexcell-basedassays
Bright Field StainedAO+Cells CountedAO+Cells
Cell Imaging• Visually check cell morphology
• Ensure only cells of interest are counted
• Archive andre-analyze cellimages
• Exportimagesforpublication
Proprietary Pattern-Recognition Software• Countindividualcellsinclusters
• Count irregular-shaped cells
• Countcellsbasedonsize
• Eliminatedebrisfrom cellcounts
Non-Fluidic Platform• Disposablecountingchambers-nowashing
• Compatiblewithfragilecells
• Maintenance-free
• RobustopticsmodulesandLEDlightsources
IQ/OQ Validation and GMP/GLP Accessories• InstallationQualificationreagents/protocol
• OperationalQualificationreagents/protocol
• On-siteIQorOQPerformance
• GMP/GLPSoftware Module
Which Instrument is Right for Me?
Features Bright Field Cell Counters Fluorescent Viability Cell Counters Image Cytometers
Mini Auto T4 Auto 1000
Auto 2000 X1 X2 K2 Spectrum Spectrum
(10x)Celigo
BFCeligo 4 Channel
Celigo 5 Channel
Cell / Sample TypeCell Line X X X X X X X X X
CulturedPrimaryCells X X X X X X X X XAlgae X
Platelets X XLow Concentration Cell Lines X X X X X X
Yeast (Clean Sample) X X XYeast (Messy Sample) X X
Primarycells(MessySample*) X X X X XPBMCs,Splenocytes,StemCells X X X X X
Hepatocytes X X X XAdipocytes*** X X X X X X
Cell-BasedAssay** X X X X X X X XApoptosis(AnnexinV-FITC/PI) X X X X XApoptosis(CaspaseActivity) X X X X X
Autophagy (CytoID-green) X XCellProliferation(CFSE) X X X X
CellCycle(PI) X X X X X X XGFPTransfection X X X X X X XRFPTransfection X X X X
MitochondrialPotential(JC-1) X X X XMulti-drug Resistance (ABC
Transporter) X X X X
Surface Marker Analysis X X X XVitality(Calcein-AM/PI) X X X X X X
Vitality (CFDA-AM) X XImage Cytometry** X X X X
* A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.** FCS Express license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter
Advantages of Cellometer Image Cytometry
![pET Express & Purify Kits User Manual - Takara Bio Manual/PT5018-1.pdf15 µl pET6xHN-C Vector (In-Fusion Ready) [100 ng/µl] 10 µl pET6xHN-GFPuv Vector [500 ng/µl] 15 µl 1.1 kb](https://img.pdfslide.net/doc/110x75/5e7b57982623d66a901d15a7/pet-express-purify-kits-user-manual-takara-bio-manualpt5018-1pdf-15-l.jpg)
![1.Set up 110 µl mix for each primer/DNA combo on ice! 1.1.1 µl 100x F primer (1 pMol/µl = 1µM final []) 2.1.1 µl 100x R primer 3.11 µl 10x PCR buffer 4.2.2](https://img.pdfslide.net/doc/110x75/56649ce05503460f949aa81d/1set-up-110-l-mix-for-each-primerdna-combo-on-ice-111-l-100x-f-primer.jpg)
