Renoprotection by alpha-Mangostin is related to the attenuation
in renal oxidative/nitrosative stress induced by cisplatin
nephrotoxicity.Prez-Rojas JM, Cruz C, Garca-Lpez P, Snchez-Gonzlez
DJ, Martnez-Martnez CM, Ceballos G, Espinosa M, Melndez-Zajgla J,
Pedraza-Chaverri J.SourceFacultad de Qumica, Departamento de
Biologa, Universidad Nacional Autnoma de Mxico, Edificio F, Mexico
City, Mexico.AbstractCisplatin (CDDP) is a chemotherapeutic agent
that produces nephrotoxicity associated with oxidative/nitrosative
stress. alpha-Mangostin (alpha-M) is a xanthone extracted from
mangosteen with antioxidant and anti-inflammatory properties. The
purpose of this study was to evaluate the renoprotective effect of
alpha-M on the CDDP-induced nephrotoxicity. alpha-M was
administered (12.5 mg/kg/day, i.g.) for 10 days (7 days before and
3 days after CDDP injection). On day 7, rats were treated with a
single injection of CDDP (7.5 mg/Kg, i.p.); 3 days after the rats
were killed. alpha-M attenuated renal dysfunction, structural
damage, oxidative/nitrosative stress, decrease in catalase
expression and increase in mRNA levels of tumour necrosis factor
alpha and transforming growth factor beta. In conclusion the
renoprotective effect of alpha-M on CDDP-induced nephrotoxicity was
associated with the attenuation in oxidative/nitrosative stress and
inflammatory and fibrotic markers and preservation of catalase
activity.http://www.ncbi.nlm.nih.gov/pubmed/19863372
The alpha-mangostin prevention on cisplatin-induced apoptotic
death in LLC-PK1 cells is associated to an inhibition of ROS
production and p53 induction.Snchez-Prez Y, Morales-Brcenas R,
Garca-Cuellar CM, Lpez-Marure R, Calderon-Oliver M,
Pedraza-Chaverri J, Chirino YI.SourceSubdireccin de Investigacin
Bsica, Instituto Nacional de Cancerologa, San Fernando No. 22,
Tlalpan, 14080 Mexico, DF, Mexico.AbstractCisplatin (CDDP) is a
widely useful chemotherapeutic agent for the treatment of tumors
including lung, ovary and testis. Acute renal injury, however, is
the main side effect observed after CDDP treatment. This side
effect is related to the apoptotic death in proximal tubular cells
in the kidney and p53 protein has a central role in this process.
On the other hand, alpha-mangostin (alpha-M), a xanthone derived
from the pericarp of mangosteen, exerts a renoprotective effect
against cisplatin-induced renal damage in rats. The aim of this
study was to evaluate whether alpha-M protects proximal tubule
renal epithelial cells (LLC-PK1) from CDDP-induced apoptotic death.
Cells were co-incubated with 5 microM alpha-M and 100 microM CDDP
for 24h. It was found that alpha-M attenuated the following
alterations: the apoptotic cell death, the increase in reactive
oxygen species (ROS), the glutathione depletion and the increase in
p53 expression induced by CDDP. In conclusion, the preventive
effect of alpha-M on CDDP-induced apoptotic death is associated to
the inhibition of p53 expression and ROS
generation.http://www.ncbi.nlm.nih.gov/pubmed/20603111
