Agilent TechnologiesBio-RadCell SignalingCorning CellgroFaster Better MediaGE-HealthcareHycloneLife TechnologiesNew England BiolabsPromegaQIAGENQuality BiologicalRoche Applied ScienceSigma-AldrichThermo Scientific FermentasUSB-Affymetrix

2014 Catalog

grcf.jhmi.edu/core-store

GRCF Mission

To provide high quality, cost effectiveresearch services and products toinvestigators throughout the JohnsHopkins Scientific Community.

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grcf.jhmi.edu

To view the GRCF Research Services Catalog flip this document.

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THE GENETIC RESOURCES CORE STORE    

CORE STORE CONTACTS 2 HOW TO ORDER FROM THE CORE STORE 2

RETURNS & REPLACEMENTS   2-­‐3  

RETURNING A STOCKED ITEM TO THE CORE STORE 2 RETURNING A SPECIAL ORDER ITEM TO THE COMPANY 2 GETTING A REPLACEMENT FROM THE COMPANY 3

WEBSITE 3

THE CORE STORE 24/7 VENDING SYSTEM 4-5

GETTING STARTED 4 WHAT TO BRING TO EACH PURCHASE 4 FIRST TIME USERS 4 SELECTING YOUR ITEMS 4 RETURNING AN ITEM PURCHASED THROUGH THE 24/7 5

STOCK PRODUCT LISTING 6-36

CORE STORE PRODUCT LISTING SORTED BY DESCRIPTION 6 AGILENT TECHNOLOGIES 20 BIO-RAD 21 CELL SIGNALING 22 CORNING CELLGRO 22 FASTER BETTER MEDIA 23 GE HEALTHCARE 23 HYCLONE 24 LIFE TECHNOLOGIES 24 NEW ENGLAND BIOLABS 28 PROMEGA 30 QIAGEN 31 QUALITY BIOLOGICAL 32 ROCHE APPLIED SCIENCE 33 SIGMA-ALDRICH 33 THERMO SCIENTIFIC FERMENTAS 35 USB-AFFYMETRIX 36

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2014 GRCF Core Store Catalog Location: East Baltimore, Blalock 1026

Phone: 410-614-1647 Fax: 410-614-9752

Web: http://grcf.jhmi.edu/core-store/ Hours: Monday through Friday

9:30 a.m. – 1:00 p.m. 2:00 p.m. – 5:00 p.m.

The Core Store offers greater than 150,000 products from the following suppliers:

Agilent Technologies Bio-Rad Cell Signaling Technology Corning Cellgro Faster Better Media GE-Healthcare

Hyclone Life Technologies New England Biolabs Promega QIAGEN Quality Biological

Roche Applied Science Sigma-Aldrich Thermo Scientific Fermentas USB-Affymetrix

For a complete listing of products and pricing go to http://grcf.jhmi.edu/core-store/

____________________________________________________________________________________

Core Store Contacts Phone Contact Email

Customer Service 410-614-1647 jhucorestore@jhmi.edu

24/7 Service 410-502-3959 Lee Hilliard Lhillia1@jhmi.edu

Website 410-614-8668 Levelle Blanchard Lblanch2@jhmi.edu

Billing 410-614-3254 Lisa Wisniewski Lwisnie1@jhmi.edu

Manager 410-614-1647 Keon Littrell Klittre1@jhmi.edu

Business Manager 410-614-5976 Kim Brown kimbrown@jhmi.edu

How to order from the Core Store

• Place your order online at http://grcf.jhmi.edu/core-store/ • Fax order to 410-614-9752 • Stop by Blalock 1026 during normal business hours • Pick-up an item from one of the 24/7 vending systems

Placing a Special Order Due to a lack of space, the store cannot stock all products offered on the website. To accommodate your needs, the Core Store will special order over 150,000 items.

• The easiest way to place a special order is on the website at http://grcf.jhmi.edu/core-store/ • Fax special order 410-614-9752. • Special orders must be placed by 10:30 a.m. for overnight delivery. • If at any time you desire a product that is not in our catalog, please do not hesitate to call us to see if we can get it

for you. Special orders may receive a discount off list price and will not include any shipping costs. If there is a product that is in high demand, let us know, so that we may consider adding it to our stock inventory.

• _______________________________________________________________________________

Returns

Returning a stocked item to the Core Store • Returns must be made within 30 days of date of purchase. • All returns must be hand delivered in original unopened and unmarked packaging to Blalock 1026. Please

remember to bring a copy of the Core Store invoice.

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Returning a special order to the company • Each supplier has specific instructions on returns. Please note that some suppliers will not accept returns. • All shipping and restocking fees will be the responsibility of the customer. • Call a Core Store customer service representative at 410-614-1647 to discuss your options.

Getting a replacement from the company • Fill out a Core Store product replacement form. The form is available for download from our website. • If you do not have a form, call the Core store and we will fax one to you. • Follow instructions on the form and email it to jhucorestore@jhmi.edu or fax it back to 410-614-9752.

All pricing and stocked inventory is subject to change. For the most current pricing please visit our website at http://grcf.jhmi.edu/core-store/

Website Web Administrator: Levelle Blanchard Phone: 410-614-8668 Email: lblanch2@jhmi.edu

The website for the Core Store is http://grcf.jhmi.edu/core-store/ The Core Store website is only to be used by Johns Hopkins employees; therefore all users need to go through JHED for authentication. We highly recommend that new customers go to JHED to view their information and add an email address if necessary. To do so:

• Go to the JHED site at http://my.jhu.edu. • Login using your JHED ID and Password and click on the Login button. • Click on the MyProfile link in the blue column on the left. • Update all empty boxes on all pages within your profile to add an email address and click Save myProfile.

Completely log out of your browser before opening up the Core Store website. If you do not know your JHED ID and password, contact JHED at 410-955-HELP or 410-516-HELP.

____________________________________________________________________________________

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The Core Store 24/7 Vending System The Core Store 24/7 is a vending system that allows access to over 200 of the most commonly used laboratory reagents 24 hours a day, 7 days a week. This service employs Pyxis technology, which utilizes RFID to dispense products in a secure environment.

24/7 Manager: Lee Hilliard Phone: 410-502-3959 Email: lhillia1@jhmi.edu 24/7 Locations East Baltimore

Blalock 1026 CRB I B02A

Bayview Asthma & Allergy Building 1A-C4

Getting Started • Fill out a vending access form. Forms are available at any 24/7 location or on the Core Store website. • Get trained by a Core Store staff member. • Start shopping. • To view a catalog of items available through the Core Store 24/7 go to http://grcf.jhmi.edu/core-store/core-store-

247/

What to bring to each purchase • Your User ID and password • Budget # to which the purchase will be charged. • Ice is not provided for refrigerated or frozen items. Please bring ice with you at the time of purchase.

First time users • THE DISPLAY SCREEN IS MAINLY A TOUCH SCREEN. • Enter your login name in the display screen. • Touch the space after PASSWORD. • Enter your temporary password, which we will issue you after you have filled out a Vending Access Form. • Touch LOGIN on the bottom of the screen. • Touch ADMIN CONTROL on the screen. • Touch DATA ADMIN on the screen. • Touch MY PREFERENCES on the screen. • Touch the PASSWORD tab on the top of the screen. • Enter your new password in the space next to New Password. NOTE: Password must be 5 to 10 characters

using no symbols. • Touch the space next to Confirm New Password and then re-enter password. • Touch ACCEPT on the bottom of the screen. • Touch MAIN MENU at top of screen to continue with a purchase or touch LOGOUT to logout. • If you forget your password, contact Lee Hilliard by calling 410-502-3959 or e-mailing lhillia1@jhmi.edu for a

temporary password.

Selecting your items • Using the Core store 24/7 Catalog, find the location of the item(s) you wish to purchase. (Example: A05, B12, C

25, etc.) The catalog is organized by item description and by manufacturer/supplier. • You might want to write all the items and locations down for your reference. • Login using your user ID and password. • Touch TOTAL ACCESS on the screen. • You will hear all the locks unlock. NOTE: THE DOORS AUTOMATICALLY LOCK AFTER 5 MINUTES OF IDLE

TIME ON THE SYSTEM. • Go to the location of the item you’re purchasing and first remove the number of items you would like to purchase. • TAKE ALL THE ITEMS YOU WILL NEED FROM THE REFRIGERATOR AND FREEZER BEFORE YOU CLOSE

THEIR DOORS. THE REASON FOR THIS IS THE DOORS AUTOMATICALLY LOCK ONCE CLOSED. IF THE DOORS LOCK AND YOU WANT SOMETHING ELSE OUT OF THE REFRIGERATOR OR FREEZER, YOU MUST LOGIN AGAIN AND BEGIN ANOTHER TRANSACTION.

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• For each quantity you take, you must hit the TAKE button on the compartment door that many times. • If you accidentally take too many products you may hit the RETURN button to make the quantity correct. • You may always touch CANCEL at bottom left corner on the display screen to cancel a transaction. • NOTE: YOU MAY ONLY PURCHASE THE NUMBER OF ITEMS THAT ARE IN STOCK. ORDERS FOR ITEMS

NOT IN STOCK MUST BE PLACED THROUGH the Core Store. • The item and quantity to be purchased will appear on the display screen. Make sure the item and quantity you are

purchasing matches up with what is on the display screen. • Once you have checked all the items and quantities you wish to purchase, touch ACCEPT on the screen.

**IMPORTANT** ONCE YOU TOUCH ACCEPT THE ORDER IS FINAL!!!. • You will then be asked to provide your method of payment, either a budget number or a Purchase Order number. • Type in your budget number or Purchase Order number. • Touch ACCEPT on the screen. • ***IMPORTANT*** When you are finished with your transaction you must logout by touching LOGOUT at the

top of the screen. • A Core Store invoice will be sent to your lab weekly confirming your purchase. Please make sure it is correct.

Returning an item purchased through the 24/7 There are three rules you must follow if you would like to return an item purchased through the Core Store 24/7.

1. You must come to the Core Store in Blalock 1026 during regular business hours (Mon-Fri 9:30 a.m. – 5 p.m. NOTE: Closed from 1-2 p.m.)

2. In order to ensure product integrity it must be maintained at the proper storage condition at all times! If an item needs to be returned please return it at the appropriate storage temperature (i.e. ice bucket for refrigerator and freezer items).

3. You must bring proof of purchase (i.e. the CORE store invoice)

If you have any questions concerning this service, please contact Lee Hilliard at lhillia1@jhmi.edu or call 410-502-3959. Thank you for using the Core Store 24/7! __________________________________________________________________________________

Store Forms A complete list of Core Store forms are available for download at http://grcf.jhmi.edu/core-store/downloads-core-store/

Supplier Catalog # Description Core Store Price

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Stock Product Listing Core Store Product Listing Sorted by Description

New England Biolabs N3232S 1 kb DNA Ladder - 100µg $36.68

Life Technologies 10787018 1 kb Plus DNA Ladder - 250µg $123.26

New England Biolabs N3232L 1 kb DNA Ladder - 500µg $146.72

New England Biolabs N3231S 100 bp DNA Ladder - 50µg $38.84

New England Biolabs N0467S 100 bp DNA Ladder, Quick Load - 125 lanes $57.54

Life Technologies 15628019 100 bp DNA Ladder - 50µg $75.39

Promega G2101 100 bp DNA Ladder - 250µl (50 lanes) $100.92

Bio-Rad 170-8352 100 bp, EZ Load Molecular Ruler - 100 lanes $112.00

New England Biolabs N3231L 100 bp DNA Ladder - 250µg $155.35

Quality Biological 351-059-101 10X RNA Gel Buffer (MOPS) - 500ml $21.54

Bio-Rad 170-6435 10X Tris Buffered Saline - 1L $37.03

Bio-Rad 161-0771 10X tris/glycine - 5L $85.75

Bio-Rad 161-0772 10X tris/glycine/sds - 5L $85.75

New England Biolabs N3200S 2-Log DNA Ladder - 100µg $38.84

New England Biolabs N3200L 2-Log DNA Ladder - 500µg $155.35

Life Technologies 21985023 2-Mercaptoethanol, 55mM - 50ml $32.64

Faster Better Media SB20-4 20X SB - 4L cube $102.00

Faster Better Media SB20-1 20X SB low conductive medium -1L $33.00

Fermentas K0171 2X PCR Master Mix- 100rxns $111.05

Bio-Rad 161-0156 30% Acryl/Bis Solution 29:1 - 500ml $55.54

Bio-Rad 161-0158 30% Acryl/Bis Solution 37.5:1 - 500ml $55.54

Bio-Rad 161-0144 40% Acryl/Bis Solution 19:1 - 500ml $66.26

Bio-Rad 161-0148 40% Acryl/Bis Solution 37.5:1 - 500ml $66.26

New England Biolabs N3236S 50 bp DNA Ladder - 100µg $44.59

Faster Better Media SB5N-8 5X SB loading medium - 8 tubes, 1.2ml per tube $23.00

Fermentas R0611 6X Loading Dye Solution - 5 x 1ml $33.22

Bio-Rad 223-9441 96 - Well 0.2 ml PCR Plates - 25/pack $199.00

Life Technologies 4346907 96-well Fast Thermal Cycling Plates, 10 $26.39

New England Biolabs R0117S Aat II - 500u $40.99

Sigma-Aldrich A6964-100ML Accutase - 100ml $29.69

Quality Biological 118-156-101 ACK Lysing Buffer - 500ml $27.05

Quality Biological 118-156-721 ACK Lysing Buffer - 4 x 100ml $43.98

Sigma-Aldrich A6013-500G Agarose, Type I - 500g $277.59

New England Biolabs R3552S AgeI-HF - 300u $48.19

Cell Signaling 9272S Akt Antibody - 100µl $207.38

Sigma-Aldrich A9647-100G Albumin Fraction V - 100g $196.10

New England Biolabs M0290S Alkaline Phosphatase,Calf Intest(CIP) - 1,000u $49.62

Bio-Rad 161-0700 Ammonium Persulfate - 10g $13.64

Sigma-Aldrich A2942-20ML Amphotericin B - 20ml $14.15

Sigma-Aldrich A9518-5G Ampicillin, Sodium Salt - 5g $24.38

Corning Cellgro 61-238-RH Ampicillin, Sodium Salt Powder - 10g $42.80

New England Biolabs M0289S Antarctic Phosphatase - 1,000u $44.59

Supplier Catalog # Description Core Store Price

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Sigma-Aldrich A2220-1ML Anti-Flag M2 AB Agarose - 1ml $384.00

Cell Signaling 7076S Anti-mouse IgG, HRP-linked Antibody - 1ml $111.93

Cell Signaling 7074S Anti-rabbit IgG, HRP-linked Antibody - 1ml $111.93

Life Technologies R96025 Anti-V5 antibody - 50µl $211.82

Sigma-Aldrich A5955-100ML Antibiotic Antimycotic (100X) - 100ml $29.74

Life Technologies 15240062 Antibiotic Antimycotic (100X) - 100ml $31.77

New England Biolabs R0114S Apa I - 5,000u $46.75

New England Biolabs R0558S Asc I - 500u $45.31

New England Biolabs P0756S ATP - (Adenosine-5 Triphosphate) 10mM - 1ml $21.58

Sigma-Aldrich A7699-1G ATP (Disodium Salt) - 1g $66.60

New England Biolabs R0174S Avr II - 100u $46.75

Life Technologies 17504044 B-27 Serum Free Supplement (50x) - 10ml $81.84

Life Technologies 12587010 B-27 Supplement w/o Vitamin A (50X) - 10ml $99.17

New England Biolabs R3136S BamH I - HF - 5,000u $39.56

Life Technologies 10748010 BENCHMARK™ Prestained Protein Ladder - 500u $171.38

Life Technologies 13256029 bFGF, Human, Recombinant - 10µg $157.90

New England Biolabs R0144S Bgl II - 2,000u $39.56

New England Biolabs C2530H BL21 Competent E. coli - 20 x 0.05ml/tube $128.74

Agilent Technologies 230132 BL21-Gold (DE3) Competent Cells - 5 x 0.2ml $179.10

Corning Cellgro 30-100-RB Blasticidin S HCl, Powder - 50mg $92.25

Life Technologies R21001 Blasticidin - 50mg $153.63

Bio-Rad 170-3965 Blot Paper, Extra Thick, 7.5x10 $52.62

Bio-Rad 170-6404 Blotting Grade Blocker-NFDM $42.87

Promega G1881 Blue/Orange Loading Dye, 6X (3X1ml) - 3ml $33.06

New England Biolabs M0367S Blunt/TA Ligase Master Mix - 50rxns $68.32

Life Technologies 11789020 BP clonase II enzyme mix - 20rxns $185.82

Sigma-Aldrich C1389-1G Carbenicillin - 1g $74.67

Cell Signaling 9662S Caspase-3 Antibody - 100µl $207.38

Promega G8091 Caspase-Glo® 3/7 Assay - 10ml $409.19

Quality Biological 118-257-711 Cell Freezing Medium B - 4 x 50ml $80.59

Cell Signaling 9803S Cell Lysis Buffer (10X) - 15ml $51.19

Promega G3580 CellTiter 96® AQueous One Assay - 1000 assays $276.54

Promega G7571 CellTiter-Glo® Luminescent Cell Viability Assay $446.37

New England Biolabs R0197S Cla I - 1,000u $43.15

Bio-Rad 170-5060 Clarity Western ECL Substrate - 200ml $119.85

Bio-Rad 170-5061 Clarity Western ECL Substrate - 500ml $228.98

Cell Signaling 9661S Cleaved Caspase-3 (Asp175) Antibody - 100µl $242.08

Agilent Technologies 600153 Cloned Pfu DNA Polymerase - 100u $100.22

Life Technologies A1048301 Collagen I, Rat Tail - 20ml $156.94

New England Biolabs P7711S ColorPlus Prestained Protein Ladder, Broad – 0.5ml $73.36

New England Biolabs P7709V ColorPlus Prestained Protein Marker, Broad – 0.5ml $51.78

Roche Applied Science 11836153001 cOmplete Mini - 25 tablets $125.94

Roche Applied Science 4693132001 cOmplete EASYpack, EDTA-free - 20 tablets $307.17

Roche Applied Science 11873580001 cOmplete EDTA-free - 20 tablets $274.36

Roche Applied Science 11836170001 cOmplete Mini, EDTA-free - 25 tablets $126.83

Supplier Catalog # Description Core Store Price

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Roche Applied Science 11697498001 cOmplete Protease Inhibitor - 20 tablets $269.28

Life Technologies C10228 Countess Chamber Slides - 50 slides $86.50

New England Biolabs M0226S CpG Methylase (Sss I Methylase) - 100u $46.75

Bio-Rad 345-0033 Criterion™ Gel Tris-HCI 4-20% 18 wells -1gel $15.00

Bio-Rad 345-0006 Criterion™ Gel, Tris-HCL 7.5%, 18 wells - 1gel $15.00

Bio-Rad 345-0034 Criterion™ Gel, Tris-HCl 4-20%, 26wells - 1gel $15.00

Bio-Rad 345-0010 Criterion™ Tris-HCL gels 10% 18-well comb 30µl $15.00

New England Biolabs C2925H dam-/dcm- Competent E.coli - 20 transformations $163.26

Roche Applied Science 10236276001 DAPI (staining reagent) - 10mg $68.67

Quality Biological 351-068-131CS DEPC Treated Water - 10 x 1L $201.68

Quality Biological 351-068-131 DEPC Treated Water - 1L $20.11

Quality Biological 351-068-721 DEPC Treated Water - 4 x 100ml $31.24

Life Technologies 15230204 Distilled Water - 10 x 500ml $75.97

Life Technologies 15230162 Distilled Water - 500ml $7.99

Corning Cellgro 25-055-CV Distilled Water - 6 x 500ml $30.75

Life Technologies 10977015 Distilled Water (Ultrapure) - 500ml $27.92

Corning Cellgro 10-014-CV DMEM - 1 gm/L glucose - 6 x 500ml $30.75

Corning Cellgro 10-013-CV DMEM - 4.5 gm/L glucose - 6 x 500ml $30.75

Life Technologies 11965092 DMEM (1X), high glucose - 500ml $7.78

Life Technologies 11965118 DMEM (1X), high glucose - 10 x 500ml $74.15

Life Technologies 11995065 DMEM (1X), high glucose w/ sodium pyruvate - 500ml $10.54

Life Technologies 11995073 DMEM (1X), high glucose - 10 x 500ml w/sodium pyruvate $76.33

Life Technologies 11885084 DMEM (1X), low glucose - 500ml $7.78

Life Technologies 11885092 DMEM (1X), low glucose - 10 x 500ml $91.59

Sigma-Aldrich D5796-500ML DMEM, high, w/L-Glutamine - 500ml $4.96

Corning Cellgro 10-092-CV DMEM/F-12 - 50/50 Mix - 6 x 500ml $49.20

Corning Cellgro 15-090-CV DMEM/F-12 - 50/50 Mix w/o L-Glutamine - 6 x 500ml $43.99

Life Technologies 11330032 DMEM/F-12, liquid (1:1) - 500ml $30.04

Life Technologies 11330057 DMEM/F-12, liquid (1:1) - 10 x 500ml $138.32

Sigma-Aldrich D2650-5X5ML DMSO - 5 x 5ml $45.61

Corning Cellgro 25-950-CQC DMSO - 250ml $52.32

Sigma-Aldrich D2650-100ML DMSO - 100ml $135.82

Quality Biological 351-028-661 DNA Gel Loading Solution (5X) - 5 x 10ml $47.73

New England Biolabs M0210S DNA Polymerase I, Klenow Fragment - 200u $42.43

Life Technologies AM1906 DNA-free - 50rxns $98.21

Sigma-Aldrich DN25-100MG DNase I - 100mg $58.71

Life Technologies 18068015 DNase I - Amplification Grade - 100u $95.82

Roche Applied Science 10104159001 DNase I (Grade II) - 100mg $57.54

New England Biolabs M0303S DNase I (RNase-Free) - 1,000u $47.47

QIAGEN 69506 DNeasy Blood & Tissue Kit - 250 $642.39

QIAGEN 69504 DNeasy Blood & Tissue Kit - 50 $149.73

Life Technologies 18427013 dNTP Mix 10 mM - 100µl $30.30

New England Biolabs N0447S dNTP Mix - 8µmol each $40.28

Sigma-Aldrich D7295-.5ML dNTP Mix (10mM) 5µmol each - .5ml $45.01

Promega U1515 dNTP Mix - 1,000µl $54.81

Fermentas R0192 dNTP Mix 10mM - 1ml $95.86

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 9

QIAGEN 201901 dNTP Mix PCR Grade - 800µl $156.49

Life Technologies 18427088 dNTP Mix 10 mM - 1ml $185.14

New England Biolabs N0446S dNTP Set - 25µmol ea. $112.20

Roche Applied Science 11969064001 dNTP Set PCR-Grade - 4 X 25µmol $186.18

Life Technologies 10297018 dNTP Set 100mM - 4 x 25µmol $189.28

Sigma-Aldrich D9663-10ML Donkey serum - 10ml $27.70

Sigma-Aldrich D9891-1G Doxycycline - 1g $40.90

Life Technologies 14200075 DPBS (10X) - 500ml $32.54

Life Technologies 14190144 DPBS (1X) w/o CaMg - 500ml $7.88

Life Technologies 14190250 DPBS (1X) w/o CaMg - 10 x 500ml $70.53

Corning Cellgro 21-031-CV DPBS (1X) w/o CaMg - 6 x 500ml $30.75

Sigma-Aldrich D8537-500ML DPBS (1X) w/o CaMg - 500ml $4.96

Quality Biological 114-059-101 DPBS with /CaMg - 500ml $8.10

New England Biolabs R0176S Dpn I - 1,000u $43.15

Agilent Technologies 500402 Dpn I - 200u $91.87

Fermentas K1081 DreamTaq™ Green PCR Master Mix (2X) - 200rxns $89.22

Fermentas R0861 DTT - 5g $74.03

Sigma-Aldrich D9163-5G DTT - 5g $56.35

Promega E2920 Dual-Glo(TM) Luciferase Assay System - 10ml $201.00

Promega E1910 Dual-Luciferase® Reporter Assay System - 100 assay $193.07

Promega E1960 Dual-Luciferase® Reporter Assay System - 10-Pack $1,233.36

Quality Biological 112-018-101 E-MEM w/L-glutamine - 500ml $8.09

GE-Healthcare RPN2109 ECL Western Blotting Detection Reagents $103.86

GE-Healthcare RPN2232 ECL Prime Western Blotting Reagent, For 1000 cm2 $160.08

GE-Healthcare RPN2106 ECL Western Blotting Detection Reagents - 2 x 250ml $226.54

New England Biolabs R3101S EcoR I HF - 10,000u $39.56

New England Biolabs R3195S EcoR V HF - 4,000u $39.56

New England Biolabs R3195L EcoR V-HF - 20,000u $158.22

Corning Cellgro 46-034-CI EDTA .5 M, pH 8.0 - 100ml $14.27

Sigma-Aldrich E7889-100ML EDTA Disodium Salt - 100ml $45.89

QIAGEN 301425 Effectene Transfection Reagent - 1ml $287.87

Quality Biological 112-018-101CS EMEM w/ L-Glutamine – 10 x 500ml $84.05

QIAGEN 12362 EndoFree Plasmid Maxi Kit - 10 $310.09

Sigma-Aldrich E9644-.2MG Epidermal Growth Factor (EGF)Human - 0.2mg $179.01

Sigma-Aldrich E1510-10ML Ethidium Bromide - 10ml $50.43

USB-Affymetrix 78200 1 ML ExoSAP-IT For PCR Product Clean-Up - 1ml $394.40

USB-Affymetrix 78200 200 UL ExoSAP-IT For PCR Product Clean-Up - 200µl $100.57

Life Technologies 4385612 Fast SYBR® Green Master Mix - 1 x 5ml $308.69

Fermentas FD0054 FastDigest® BamH I - 800rxns $49.35

Fermentas FD0084 FastDigest® Bgl II - 200rxns $49.35

Fermentas FD0274 FastDigest® EcoRl - 800rxns $49.35

Fermentas FD0594 FastDigest® Not 1 - 50rxns $63.59

Fermentas FD0684 FastDigest® Xba I - 300rxns $58.84

Fermentas FD0694 FastDigest® Xho I - 400rxns $49.35

Corning Cellgro 35-010-CV Fetal Bovine Serum - 500ml $117.71

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 10

Sigma-Aldrich F6178-500ML Fetal Bovine Serum, Qualified - 500ml $124.91

Sigma-Aldrich F2442-500ML Fetal Bovine Serum, U.S. origin - 500ml $135.85

Life Technologies 26140079 Fetal Bovine Serum, U.S. Qualified - 500ml $183.56

Hyclone SH30071.03 Fetal Bovine Serum, Characterized - 500ml $193.43

Life Technologies 16000044 Fetal Bovine Serum, Certified - 500ml $239.60

Hyclone SH30070.03 Fetal Bovine Serum, Defined - 500ml $273.47

Corning Cellgro 35-011-CV Fetal Bovine Serum, Heat Inactivated - 500ml $129.60

Sigma-Aldrich F4135-500ML Fetal Bovine Serum, Heat Inactivated - 500ml $164.47

Life Technologies 16140071 Fetal Bovine Serum, Qual, Heat Inactivated - 500ml $187.17

Sigma-Aldrich F0291-25UG Fibroblast growth factor - 25µg $219.88

Sigma-Aldrich F2006-1MG Fibronectin - 1mg $96.66

Sigma-Aldrich F1141-2MG Fibronectin - 2mg $176.40

GE-Healthcare 17-1440-02 Ficoll-Paque™ PLUS - 6 x 100ml $164.66

GE-Healthcare 17-1440-03 Ficoll-Paque™ PLUS - 6 x 500ml $565.00

Sigma-Aldrich F9037-100ML Formamide - 100ml $32.20

Roche Applied Science 11814320001 Formamide, Liquid - 500ml $59.44

Sigma-Aldrich F7503-1L Formamide - 1L $86.47

Promega E2693 FuGENE® 6 Transfection Reagent - .5ml $219.00

Promega E2691 FuGENE® 6 Transfection Reagent - 1ml $380.00

Promega E2311 FuGENE® HD Transfection Reagent - 1ml $449.00

Corning Cellgro 61-234-RF G418 - 1g $65.40

Corning Cellgro 61-234-RG G418 - 5g $187.86

Agilent Technologies 200049 G418 Sterile Solution - 10ml 50mg/ml $72.38

Corning Cellgro 30-234-CR G418 Sulfate Solution - 20ml $85.61

Life Technologies 11791020 GATEWAY®LR clonase II enzyme mix - 20rxns $214.00

New England Biolabs B7021S Gel Loading Dye Blue (6x) - 6ml $31.64

Life Technologies A1413302 Geltrex LDEV-Free hESC-qualified - 5ml $193.52

Life Technologies A1413202 Geltrex LDEV-free RGF - 5ml $174.27

Bio-Rad 165-2089 Gene Pulser® Cuvette, 0.1cm - 50pk $169.55

Life Technologies N8080007 GeneAmp® dNTP's - (4 x 320µl of 10mM soln) $45.17

Fermentas K0692 GeneJET™ Gel Extraction Kit - 250preps $314.16

Fermentas K0691 GeneJET™ Gel Extraction Kit - 50preps $70.23

Fermentas K0503 GeneJET™ Plasmid Miniprep Kit - 250preps $257.21

Fermentas K0502 GeneJET™ Plasmid Miniprep Kit - 50preps $62.64

Sigma-Aldrich NA0300-1KT GenElute™ HP Plasmid Maxi Kit - 10preps $158.62

Sigma-Aldrich PLN70-1KT GenElute™ Plasmid miniprep Kit - 70preps $98.30

Fermentas SM0323 GeneRuler™ 100bp DNA Ladder Plus, RTU- 100apps $73.08

Fermentas SM0243 GeneRuler™ 100bp DNA Ladder, RTU - 100apps $60.74

Fermentas SM0313 GeneRuler™ 1kb DNA Ladder - 500apps $152.81

Fermentas SM1333 GeneRuler™ 1kb DNA Ladder Plus – 500apps $164.20

Life Technologies 10131035 Geneticin® - 20ml $117.46

Life Technologies 11811023 Geneticin® Selective Antibiotic, powder - 1g $77.34

Life Technologies 11811031 Geneticin® Selective Antibiotic, powder - 5g $234.75

Sigma-Aldrich WGA2-50RXN GenomePlex® Complete WGA Kit- 50rxns $298.49

Quality Biological 120-098-661 Gentamicin,50mg/ml - 5 x 10ml $106.27

New England Biolabs E2611S Gibson Assembly Master Mix - 10rxns $112.91

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Life Technologies 35050061 GlutaMAX-I Supplement - 100ml $25.74

GE-Healthcare 17-0756-01 Glutathione Sepharose™ - 10ml $261.00

Sigma-Aldrich G6279-1L Glycerol, SigmaUltra - 1L $79.38

Bio-Rad 161-0718 Glycine - 1kg $50.00

Fermentas R0561 Glycogen - 0.5ml $55.05

Roche Applied Science 10901393001 Glycogen - 20mg $125.70

Sigma-Aldrich G9023-10ML Goat Serum - 10ml $27.70

Promega A5003 GoScript™ Reverse Transcriptase - 100rxns $107.82

Promega M7123 GoTaq Green Master Mix - 1000rxns $283.50

Promega M3008 GoTaq® DNA Polymerase - 2,500u $467.48

Promega M3005 GoTaq® DNA Polymerase - 500u $105.56

Promega M8291 GoTaq® Flexi DNA Polymerase - 100u $24.13

Promega M8295 GoTaq® Flexi DNA Polymerase - 500u $105.56

Promega M7122 GoTaq® Green Master Mix - 100rxns $32.42

Corning Cellgro 10-080-CV Ham's F-12 w/L-Glut - 6 x 500ml $46.40

Corning Cellgro 21-021-CV HBSS (1X) w/o CaMg - 6 x 500ml $30.75

Life Technologies 14170161 HBSS (1X) w/o CaMg -10 x 500ml $74.24

Life Technologies 14170112 HBSS (1X) w/o CaMg- - 500ml $8.31

Life Technologies 14175103 HBSS (1X) w/o phenol red - 10 x 500ml $77.95

Life Technologies 14175095 HBSS (1X) w/o phenol red - 500ml $8.51

Sigma-Aldrich H9394-500ML HBSS w/o CaMg - 500ml $4.96

Corning Cellgro 21-022-CV HBSS w/o CaMg & Phenol Red - 6 x 500ml $30.75

Quality Biological 114-062-101 HBSS w/o phenol red & CaMg - 500ml $9.89

Sigma-Aldrich H4034-100G Hepes - 100g $52.04

Corning Cellgro 25-060-CI Hepes Buffer (1M) - 6 x 100ml $117.71

Life Technologies 15630080 Hepes Buffer Soln (1M) - 100ml $39.53

Quality Biological 118-089-721 Hepes Buffer Soln (1M) - 4 x 100ml $93.26

Agilent Technologies 600675 Herculase® II Fusion DNA Polymerase - 40rxns $48.26

Agilent Technologies 600677 Herculase® II Fusion with dNTPs Combo - 200rxns $215.30

Life Technologies 4368814 High Capacity cDNA Kit - 200rxns $188.79

New England Biolabs R3104S HINDIII-HF - 10,000u $39.56

QIAGEN 12662 HiSpeed Plasmid Maxi Kit (10) $256.96

QIAGEN 12663 HiSpeed Plasmid Maxi Kit (25) $619.21

QIAGEN 12643 HiSpeed Plasmid Midi Kit (25) $309.12

Sigma-Aldrich H8889-500ML Histopaque®-1077 - 500ml $158.91

QIAGEN 203205 HotStarTaq DNA Polymerase - 1000u $510.05

Sigma-Aldrich H1009-100ML Hydrogen Peroxide - 100ml $40.60

Corning Cellgro 30-240-CR Hygromycin B Solution - 1 X 20ml $118.90

GE-Healthcare 28-9068-35 Hyperfilm ECL 5x7in - 50 Sheets $199.52

GE-Healthcare 28-9068-38 Hyperfilm ECL 8x10in - 50 Sheets $235.71

GE-Healthcare 28-9068-39 Hyperfilm ECL 8x10in - 100 Sheets $393.47

Life Technologies IB301031 iBlot® Transfer Stack, Regular 3 x 10 sets $444.81

Life Technologies IB301001 iBlot® Transfer Stack, Regular - 10 sets $177.16

Quality Biological 112-035-101 IMDM - 500ml $17.61

Life Technologies 12440053 IMDM (1X) - 500ml $24.24

Supplier Catalog # Description Core Store Price

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Life Technologies 12440061 IMDM (1X) - 10 x 500ml $179.19

Bio-Rad 162-0174 ImmunBlot PVDF Membrane – 10pk $79.90

Bio-Rad 162-0177 ImmunBlot PVDF, 26cm x 3.3m - 1roll $294.27

New England Biolabs M0370S Instant Sticky-end Ligase Master Mix - 50rxns $68.32

Life Technologies 12585014 Insulin, Human Recombinant, Zinc Solution - 5ml $37.55

Life Technologies LC2005 Invitrolon PVDF/Filter - 20pk $185.82

Corning Cellgro 46-102-RF IPTG - 1g $23.58

Fermentas R0391 IPTG - 1g $41.76

Life Technologies 15529019 IPTG - 1g $54.22

Bio-Rad 170-8880 iQ SYBR Green Supermix - 100 x 50µl $178.32

Bio-Rad 170-8882 iQ SYBR Green Supermix - 500 x 50µl $819.00

Bio-Rad 170-8841 iScript RT Supermix - 100rxns $465.00

Bio-Rad 170-8840 iScript RT Supermix - 25rxns $150.00

Bio-Rad 170-8890 iScript™ cDNA Synthesis Kit - 25rxns $135.00

Bio-Rad 170-8891 iScript™ cDNA Synthesis Kit - 100rxns $394.63

Sigma-Aldrich I9516-500ML Isopropanol - 500ml $47.14

Bio-Rad 172-5122 iTaq Univeral SYBR Green SMX - 10ml $498.89

Bio-Rad 172-5121 iTaq Univeral SYBR Green SMX - 5ml $259.19

Sigma-Aldrich I3146-5ML ITS Liquid Supplement - 5ml $25.53

Corning Cellgro 25-800-CR ITS(insulin-Transferrin Selenium)100x Sol. 10ml $32.10

Promega L2001 JM109 Competent Cells - 1ml (5 x 200µl) $124.61

Sigma-Aldrich D8187-250UN JumpStart Red Taq DNA - 250u $44.61

Bio-Rad 161-0324 Kaleidoscope™ Prestained Standards - 500µl $115.00

Sigma-Aldrich K0879-50MG Kanamycin - 50mg $19.85

Life Technologies 17005042 Keratinocyte-SFM with supplements - 500ml $82.80

Life Technologies 10829018 Knockout™ DMEM - 500ml $26.09

Life Technologies 10828028 Knockout™ Serum - 500ml $266.70

New England Biolabs R3142S Kpn I- HF - 4,000u $43.15

Life Technologies 25030081 L-Glutamine 200mM (100X) - 100ml $16.29

Corning Cellgro 25-005-CI L-Glutamine - 6 x 100ml $42.00

Quality Biological 118-084-721 L-Glutamine 200mM, - 4 x 100ml $46.43

Bio-Rad 161-0737 Laemmli Sample Buffer - 30ml $13.64

Sigma-Aldrich L2020-1MG Laminin - 1mg $94.19

Life Technologies 23017015 Laminin, Mouse, Natural - 1mg $112.65

Sigma-Aldrich L0543-10EA LB Agar Kanamycin-50µg/ml, plates - 10ea $25.22

Quality Biological 340-147-231 LB Agar Plates w/50µg/ml kanamycin (25ml fill)- 10ea $22.59

Quality Biological 340-108-231 LB Agar Plates,100 µg/ml Ampicillin (25ml fill) - 10ea $23.77

Quality Biological 340-107-231 LB Agar Plates w/50mcg Ampicillin (25ml fill) - 10ea $23.66

Sigma-Aldrich L2542-500ML LB Broth - 500ml $18.55

Quality Biological 340-004-101CS LB Broth (Lauria Bertani) – 10 x 500ml $79.94

Quality Biological 340-004-101 LB Broth (Laurie Bertani) - 500ml $8.39

Life Technologies 11415064 Leibovitz's L-15 Medium with/phenol (1X) - 500ml $30.04

Life Technologies 18263012 LIBRARY Efficiency™ DH5Alpha™ Competent Cells - 1ml $129.02

Roche Applied Science 4707494001 LightCycler 480 Probes Master $260.13

Life Technologies 11668027 Lipofectamine™ 2000 Transfection Reagent - .75ml $274.92

Life Technologies 11668019 Lipofectamine™ 2000 Transfection Reagent - 1.5ml $508.36

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 13

Life Technologies 15338100 Lipofectamine™ LTX and Plus Reagent - 1ml $482.56

Life Technologies 13778075 Lipofectamine™ RNAiMAX - 0.75ml $367.41

Promega E1501 Luciferase Assay Reagent – 10pk $595.96

Promega E1500 Luciferase Assay System - 100assays $88.16

Corning Cellgro 25-072-CI Lymphocyte separation medium – 1 x 100ml $11.89

Promega M1701 M-MLV Reverse Transcriptase - 10,000u $48.26

New England Biolabs M0368S M-MuLV Reverse Transcriptase - 4,000u $58.97

New England Biolabs M0368L M-MuLV Reverse Transcriptase - 10,000u $117.95

Life Technologies LC5602 Magic Mark XP Standard - 250µl $255.14

Quality Biological 351-033-721 Magnesium Chloride 1m - 4 x 100ml $34.74

Life Technologies 18258012 MAX Efficiency DH5Alpha™ Competent Cells - 1ml $171.27

Fermentas K0222 Maxima™SYBR Green qPCR Master Mix (2X) -10 x 1.25ml $615.97

Fermentas K0221 Maxima™SYBR Green qPCR Master Mix (2X) -2 x 1.25ml $145.21

Life Technologies 16600082 McCoy's 5A Medium (1X) - 500ml $23.06

Life Technologies 16600108 McCoy's 5A Medium (1X) - 10 x 500ml $139.94

Corning Cellgro 10-050-CV McCoy's 5A w/L-Glut - 6 x 500ml $54.69

Sigma-Aldrich M7145-100ML MEM NEAA (100x) - 100ml $14.57

Life Technologies 11140050 MEM NEAA (100X) 10mM - 100ml $15.98

Corning Cellgro 10-010-CV MEM w/ Earle’s salts & L-glutamine (1X) - 6 x 500ml $30.75

Life Technologies 11095080 MEM w/ L-glutamine (1X) - 500ml $8.54

Life Technologies 11095098 MEM w/ L-glutamine (1X) - 10 x 500ml $71.97

New England Biolabs R3589S Mfe I - HF - 500u $48.91

Life Technologies 4346906 MicroAmp® Fast Optical 96-Well Plate - 20plates $63.52

Life Technologies N8010560 MicroAmp® Optical 96-Well Reaction Plate-10/bx $49.58

Life Technologies 4311971 MicroAmp® Optical Adhesive Film - 100covers $165.13

Life Technologies 4306737 MicroAmp™ Optical 96 Well Plate - 20plates $101.04

Bio-Rad MSB-1001 Microseal B Seals – 100pk $153.00

Bio-Rad 170-4157 Midi PVDF Transfer pack – 10pk $194.00

Bio-Rad 456-1034 Mini Protean® TGX™ 10% 10well 50µl - 10gels $96.30

Bio-Rad 456-1033 Mini Protean® TGX™ 10%, 10-well 30µL - 10gels $103.00

Bio-Rad 456-1043 Mini Protean® TGX™ 12%, 10-well 30µL - 10gels $103.00

Bio-Rad 456-1083 Mini Protean® TGX™ 4-15%, 10-well 30ul - 10gels $96.30

Bio-Rad 456-1086 Mini Protean® TGX™ 4-15%, 15-well 15µl - 10gels $96.30

Bio-Rad 456-1084 Mini Protean® TGX™ 4-15%, 50µl 10well - 10gels $96.30

Bio-Rad 456-1093 Mini Protean® TGX™ 4-20%, 10-well 30ul - 10gels $96.30

Bio-Rad 456-1096 Mini Protean® TGX™ 4-20%,15-well 15µl - 10gels $96.30

Bio-Rad 456-1023 Mini Protean® TGX™ 7.5%, 10-well 30µl - 10gels $103.00

Bio-Rad 170-3932 Mini Trans-Blot Filter Paper $44.82

QIAGEN 217004 miRNeasy Mini Kit (50) $327.47

Life Technologies AM1561 mirVana™ miRNA Isolation Kit - 40purifications $237.81

Sigma-Aldrich M4287-2MG Mitomycin C - 2mg $74.98

New England Biolabs R0198S Mlu I - 1,000u $43.15

GE-Healthcare NA931-1ML Mouse IgG, HRP-Linked Whole Ab (sheep) -1ml $254.68

Life Technologies 4311235 MultiScribe™ Reverse Transcriptase - 100µl $70.91

Life Technologies N8080018 MuLV Reverse Transcriptase - 5,000u $58.95

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 14

Life Technologies 17502048 N-2 Supplement - 5ml $67.40

Sigma-Aldrich N6530-5ML N1 Medium Supplement (100X) - 5ml $24.60

Roche Applied Science 11383213001 NBT Solution - 3ml $54.29

New England Biolabs R3193S NcoI - HF - 1,000u $41.71

New England Biolabs R0111S Nde I - 4,000u $43.15

New England Biolabs C2987I NEB 5-alpha Competent E.coli - 6 X 0.2 ml $103.56

New England Biolabs C2987H NEB 5-Alpha Competent Cells - 20 x .05ml $132.33

New England Biolabs C2992H NEB 5-alpha Competent E.coli - 20 transformations $132.33

New England Biolabs C2989K NEB 5-alpha Electrocompetent e. coli - 6 x .1mL $122.98

New England Biolabs C2984H NEB Turbo Competent E.coli - 20 x .05ml/tube $174.05

Life Technologies 21103049 Neurobasal-SFM - 500ml $55.84

Sigma-Aldrich N3910-24ML Neutralization Solution B - 24ml $50.23

New England Biolabs R3131S NheI-HF - 1,000u $45.31

QIAGEN 30210 Ni-NTA Agarose (25ml) $268.55

Life Technologies LC2000 Nitrocellulose and filter paper (.2um;8.3x7.5cm) $148.27

Bio-Rad 162-0115 Nitrocellulose, 0.45,33cm x 3M - 1roll $249.45

Quality Biological 114-055-101 Normal Saline - 500ml $7.10

New England Biolabs R3189S Not I-HF - 500u $48.91

New England Biolabs R3189L Not I-HF - 2,500u $195.62

Life Technologies LC5800 Novex sharp PS prot STD - 2x250µl $141.53

New England Biolabs R0127S Nsi I - 1,000u $43.15

QIAGEN 129115 Nuclease-Free Water - 1L $46.08

Promega P1193 Nuclease-Free Water - 50ml (2 x 25ml) $44.37

Life Technologies NP0321BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 10 well - 10gels $136.72

Life Technologies NP0322BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 12 wel l - 10gels $136.72

Life Technologies NP0323BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 15 well - 10gels $136.72

Life Technologies NP0335BOX NuPAGE® 4-12% Bis-Tris Gel 1.5 mm, 10 well - 10gels $136.72

Life Technologies NP0005 NuPAGE® Antioxidant - 15ml $24.45

Life Technologies NP0007 NuPAGE® LDS Sample Buffer (4X) - 10ml $12.76

Life Technologies NP0002 NuPAGE® MES SDS Running Buffer(B-T Gels 20X) - 500ml $68.85

Life Technologies NP0001 NuPAGE® MOPS SDS Running Buffer(B-T Gels)(20X) – 500ml $68.85

Life Technologies NP0004 NuPAGE® Sample Reducing Agent (10X) - 250µl $12.76

Life Technologies NP0006 NuPAGE® Transfer Buffer (20X) - 125ml $21.63

Life Technologies NP00061 NuPAGE® Transfer Buffer 20X - 1L $150.20

Life Technologies 18418012 Oligo(dT)12-18 Primer - 25µg $77.02

Promega C1101 Oligo(dT)15 Primer - 20µg $56.20

QIAGEN 205111 Omniscript RT Kit (50) $235.70

Life Technologies C737303 One Shot® Stabl 3 Comp E coli $360.41

Life Technologies C404003 One Shot® TOP10 - 20rxn $331.89

New England Biolabs M0480L OneTaq DNA Polymerase – 1000u $115.07

New England Biolabs M0480S OneTaq DNA Polymerase – 200u $28.77

New England Biolabs M0481L OneTaq Hot Start DNA Polymerase – 1000u $238.77

Life Technologies 31985088 Opti-MEM I - 10 x 500ml $348.53

Life Technologies 31985070 Opti-MEM I - 500ml $40.05

Bio-Rad TCS-0803 Optical Flat 8-Cap Strips - 120strips $36.00

New England Biolabs R0547L Pac I - 1,250u $184.12

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 15

New England Biolabs R0547S Pac I – 250u $46.03

Fermentas 26619 PageRuler™ Prestained Protein Ladder Plus,RTU - 100apps $125.28

Fermentas 26616 PageRuler™ Prestained Protein Ladder, RTU -1 00apps $104.40

Fermentas 26614 PageRuler™ Unstained Protein Ladder - 100apps $83.52

Sigma-Aldrich P6148-500G Paraformaldehyde - 500g $27.49

USB-Affymetrix 19943 1 LT Paraformaldehyde Solution, 4% in PBS - 1L $49.30

Quality Biological 114-058-101 PBS (1X) pH 7.4 w/o CaMg - 500ml $6.98

Life Technologies 10010023 PBS (1X) w/o CaMg, liquid - 500ml $7.46

Corning Cellgro 21-040-CV PBS (1X) w/o CaMg - 6 x 500ml $30.75

Quality Biological 114-058-101CS PBS (1X) pH 7.4 w/o CaMg – 10 x 500ml $69.88

Life Technologies 10010049 PBS (1X) w/o CaMg, liquid - 10 x 500ml $72.38

Quality Biological 119-069-131 PBS 10X pH 7.4 – 1L $21.72

Corning Cellgro 46-013-CM PBS 10X, pH 7.4 - 6 x 1L $73.72

Roche Applied Science 11666789001 PBS Buffer (10X), premixed - 4L $126.67

Quality Biological 119-069-131CS PBS 10X pH 7.4 - 10 x 1L $216.50

Promega M7502 PCR Master Mix – 100rxns $64.09

Sigma-Aldrich P0781-100ML Penicillin-Streptomycin - 100ml $10.19

Life Technologies 15140122 Penicillin Streptomycin - 100ml $18.91

Corning Cellgro 30-001-CI Penicillin Streptomycin - 6 x 100ml $38.05

Quality Biological 120-095-721 Penicillin-Streptomycin 100x - 4 x 100ml $38.38

Life Technologies 15140163 Penicillin Streptomycin - 20 x 100ml $213.57

Sigma-Aldrich P5318-5MG Pepstatin A - 5mg $51.30

GE-Healthcare 17-0891-01 Percoll® (Sterile) - 1L $380.00

Agilent Technologies 600250 PfuTurbo® DNA Polymerase - 100u $103.94

Agilent Technologies 600252 PfuTurbo® DNA Polymerase - 500u $425.02

Agilent Technologies 600380 PfuUltra™ High-fidelity DNA Polymerase - 100u $116.00

Agilent Technologies 600670 PfuUltra™ II Fusion HS DNA Polymerase - 40rxn $96.51

Promega A1360 pGEM®-T Easy Vector System I - 20rxns $160.72

Life Technologies 15593031 Phenol : Chloroform:(25:24:1,v/v) - 100ml $98.21

Sigma-Aldrich P4557-400ML Phenol- Tris Saturated, Equilibrated - 400ml $117.37

Sigma-Aldrich P3803-100ML Phenol: Chloroform 25:24:1 - 100ml $59.95

Sigma-Aldrich P0044-1ML Phosphatase Inhibitor Cocktail 3 - 1ml $62.90

Sigma-Aldrich P5726-1ML Phosphatase Inhibitor Cocktail II - 1ml $57.50

Cell Signaling 4060S Phospho-Akt (Ser473) (D9E) XP(tm) Rabbit mAb-100µl $268.11

Cell Signaling 9271S Phospho-Akt (Ser473) Antibody - 100µl $242.08

Cell Signaling 4370S Phospho-p44/42 MAPK (Erk1/2) Rabbit mAb - 200µl $268.11

Roche Applied Science 4906837001 PhosSTOP - 20 tablets $226.35

Roche Applied Science 4906845001 PhosSTOP - 10 tablets $115.47

New England Biolabs M0530S Phusion High-Fidelity DNA Polymerase - 100u $75.52

New England Biolabs M0530L Phusion High-Fidelity DNA Polymerase - 500u $302.06

New England Biolabs M0531S Phusion High-Fidelity PCR Master Mix - 100rxns $122.26

New England Biolabs M0531L Phusion High-Fidelity PCR Master Mix - 500rxns $489.06

Life Technologies 11306016 PLATINUM® PCR Super Mix - 100rxns $105.56

Life Technologies 11708013 PLATINUM® PFX DNA polymerase - 100u $126.13

Life Technologies 10966018 PLATINUM® Taq DNA Polymerase - 100u $95.32

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 16

Life Technologies 10966034 PLATINUM® Taq DNA Polymerase - 500u $362.06

Life Technologies 11304011 PLATINUM® Taq DNA Polymerase High Fidelity - 100u $158.86

New England Biolabs R0560S Pme I - 500u $48.91

Sigma-Aldrich P6407-5MG Poly-D-Lysine - 5mg $32.41

Sigma-Aldrich P1024-100MG Poly-D-Lysine - 100mg $147.26

Sigma-Aldrich P1524-100MG Poly-L-Lysine - 100mg $129.50

QIAGEN 301107 PolyFect Transfection Reagent (4 x 1 ml) $522.61

Life Technologies 4367659 Power SYBR® Green PCR Master Mix (1 x 5ml) - 200rxns $275.62

Bio-Rad 161-0373 Precision Plus All Blue Standards - 500µl, - 50apps $94.52

Bio-Rad 161-0375 Precision Plus Protein Kaleidoscope Standard - 500µl $126.67

Bio-Rad 161-0374 Precision Plus Protein Standards - 500µl $116.93

Bio-Rad 161-0734 Premixed Buffers, 10x Tris/Glycine - 1L $26.31

Bio-Rad 161-0732 Premixed Running Buffer - 1L $26.31

Bio-Rad 161-0318 Prestained STDS, Broad Range - 500µl $97.44

Life Technologies P36930 ProLong Gold Antifade - 10ml $136.72

Cell Signaling 9071S ProLong(R) Gold Antifade Reagent - 10ml $137.96

Cell Signaling 8961S ProLong(R) Gold Antifade Reagent with DAPI - 10ml $146.64

Life Technologies P36931 Prolong Gold Antifade Reagent - 10ml $159.00

Sigma-Aldrich P2714-1BTL Protease Inhibitor - 1 bottle $41.99

Sigma-Aldrich P8340-1ML Protease Inhibitor Cocktail - 1ml $65.70

Sigma-Aldrich P8340-5ML Protease Inhibitor Cocktail - 5ml $139.76

Cell Signaling 5872S Protease/Phosphatase Inhibitor Cocktail (100X) - 1ml $207.38

Bio-Rad 500-0006 Protein Assay Dye Reagent - 450ml $109.13

GE-Healthcare 17-0618-01 Protein G Sepharose™ 4 Fast Flow - 5ml $509.00

New England Biolabs P7703S Protein Ladder (10-250 kDa) – 100 gel lanes $53.94

New England Biolabs P7702S Protein Marker, Broad Range 150 lanes $53.22

Fermentas EO0491 Proteinase K - 1ml $42.71

QIAGEN 19131 Proteinase K - 2ml $81.43

Roche Applied Science 3115879001 Proteinase K - 100mg $90.02

Life Technologies 25530015 Proteinase K - 100mg $92.85

Roche Applied Science 3115828001 Proteinase K Solution, PCR Grade - 5ml $142.91

New England Biolabs R3140S Pst l-HF -10,000u $43.15

Life Technologies K210014 PureLink HiPure Plasmid Filter Midiprep Kit - 25preps $225.28

Life Technologies K210016 PureLink™ HiPure Filter Maxi - 10preps $187.18

Life Technologies K210012 PureLink™ Quick Gel Extraction Kit - 50preps $76.20

Life Technologies K210011 PureLink™ Quick Plasmid Miniprep Kit - 250preps $264.21

Life Technologies K210010 PureLink™ Quick Plasmid Miniprep Kit - 50preps $59.64

Sigma-Aldrich P8833-25MG Puromycin - 25mg $66.06

New England Biolabs R3150S Pvul-HF - 500u $48.19

New England Biolabs M0492S Q5 High-Fidelity 2X Master Mix - 100rxns $120.11

New England Biolabs M0491S Q5 High-Fidelity DNA Polymerase – 100u $74.08

New England Biolabs M0494S Q5 Hot Start High-Fidelity 2X Master Mix - 100rxns $140.24

New England Biolabs M0493S Q5 Hot Start High-Fidelity DNA Polymerase - 100u $89.90

New England Biolabs E0554S Q5 Site-Directed Mutagenesis Kit - 10rxns $129.46

QIAGEN 51104 QIAamp DNA Blood Mini Kit (50) $137.17

QIAGEN 51304 QIAamp DNA Mini Kit (50) $151.66

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 17

QIAGEN 20021 QIAEX II Gel Extraction Kit (150) $162.29

QIAGEN 12162 QIAGEN Plasmid Maxi Kit (10) $216.38

QIAGEN 12163 QIAGEN Plasmid Maxi Kit (25) $512.95

QIAGEN 12143 QIAGEN Plasmid Midi Kit (25) $250.19

QIAGEN 12123 QIAGEN Plasmid Mini Kit (25) $188.37

QIAGEN 27104 QIAprep Spin Miniprep Kit (50) $84.24

QIAGEN 27106 QIAprep Spin Miniprep Kit (250) $354.52

QIAGEN 28704 QIAquick Gel Extraction Kit (50) $104.33

QIAGEN 28706 QIAquick Gel Extraction Kit (250) $496.52

QIAGEN 28104 QIAquick PCR Purification Kit (50) $104.33

QIAGEN 28106 QIAquick PCR Purification Kit (250) $496.52

QIAGEN 79654 QIAshredder (50) $75.06

QIAGEN 79656 QIAshredder (250) $322.64

QIAGEN 204443 QuantiTect Probe RT-PCR Kit (200) $600.85

QIAGEN 205313 QuantiTect Rev. Transcription Kit (200) $1,068.40

QIAGEN 205311 QuantiTect Rev. Transcription Kit (50) $313.95

QIAGEN 204143 QuantiTect SYBR Green PCR Kit (200) $411.52

Bio-Rad 732-6120 Quantum Prep™ Midiprep Kit - 20 preps $174.42

New England Biolabs E0542S Quick Blunting™ Quick Ligation Kit - 20rxns $110.76

New England Biolabs M2200S Quick Ligation Kit - 30rxns $69.76

Agilent Technologies 210518 QuikChange Lightning - 10rxns $234.78

Agilent Technologies 200521 QuikChange® II XL site-directed mutagenesis kit $244.06

GE-Healthcare NA934-1ML Rabbit IgG, HRP-linked Ab (donkey) - 1ml $200.05

Sigma-Aldrich F7425-.2MG Rabbit-Anti flag - 0.2mg $287.50

GE-Healthcare RPN800E Rainbow™ Molecular Weight Marker IMPROVED-250µl $108.46

Life Technologies N8080127 Random Hexamers (50µm) - 1kit $62.37

Promega C1181 Random Primers - 20µg $36.48

Life Technologies 48190011 Random Primers - 9 A260u $77.02

Roche Applied Science 11635379001 Rapid DNA Ligation Kit - 40rxns $156.60

Fermentas K1422 Rapid DNA Ligation Kit - 50rxns $118.64

Promega N2511 Recombinant RNasin® Ribonuclease Inhibitor - 2,500u $100.04

Life Technologies 12648010 Recovery Cell Culture Medium-50ml $110.72

Sigma-Aldrich R4775-1.2ML Redextract-N-amp kit - 1.2ml $102.34

Sigma-Aldrich XNAT-100RXN REDExtract-N-Amp™ Tissue PCR Kit - 100rxns $227.00

Sigma-Aldrich R2523-100RXN RedTaq Ready Mix PCR Mix – 100rxns $58.65

Fermentas EO0381 RiboLock™ RNase Inhibitor - 2,500u (40u/µl) $50.30

Life Technologies 18021071 Ribonuclease H - 120u $286.76

Sigma-Aldrich R0278-50ML Ripa Buffer - 50ml $45.14

Cell Signaling 9806S Ripa Buffer (10x) - 15ml $51.19

Sigma-Aldrich R0901-100ML RNAlater - 100ml $101.17

QIAGEN 76104 RNAlater RNA Stabilization Reagent $70.61

QIAGEN 19101 RNase A - 17,500u $184.51

Fermentas EN0531 RNase A (DNase & protease free) - 1ml(10mg/ml) $37.96

New England Biolabs M0297S RNase H - 250u $46.03

New England Biolabs M0307S RNase Inhibitor - 2,000u $53.94

Supplier Catalog # Description Core Store Price

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Life Technologies N8080119 RNase Inhibitor - 2,000u $60.24

New England Biolabs M0307L RNase Inhibitor - 10,000u $215.76

QIAGEN 79254 RNase-Free DNase Set (50) $91.09

Life Technologies 10777019 RNaseOut™ - 5000u $133.91

Sigma-Aldrich R2020-250ML RNaseZap - 250ml $45.17

Promega N2115 RNasin® Ribonuclease Inhibitor - 10,000u $436.77

Promega N2111 RNasin® Ribonuclease Inhibitor - 2,500u $177.75

QIAGEN 74804 RNeasy Lipid Tissue Mini Kit (50) $341.96

QIAGEN 74106 RNeasy Mini Kit (250) $1,171.76

QIAGEN 74104 RNeasy Mini Kit (50) $266.62

QIAGEN 74134 RNeasy Plus Mini Kit (50) $300.43

Sigma-Aldrich R8758-500ML RPMI-1640 Medium - 500ml $4.96

Life Technologies 11875093 RPMI 1640 (1X) w/ L-Glutamine - 500ml $7.78

Quality Biological 112-024-101 RPMI 1640 w/o L-Glutamine - 500ml $7.98

Life Technologies 61870036 RPMI 1640 w/ Glutamax - 500ml $13.78

Corning Cellgro 10-040-CV RPMI 1640 w/L-Glutamine - 6 x 500ml $30.75

Life Technologies 11875119 RPMI 1640 (1X) w/ L-Glutamine - 10 x 500ml $74.15

Life Technologies 11875135 RPMI 1640 (1X) w/ L-Glutamine - 6 x 1L $75.59

QIAGEN 330401 RT2 First Strand cDNA Kit - 12rxns (20µl each) $229.91

Life Technologies 15544034 S.O.C. Medium - 10 x 10ml $86.42

New England Biolabs R3156S Sac I HF - 2,000u $39.56

New England Biolabs R0157S Sac II - 2,000u $41.71

New England Biolabs R3138S Sal I HF - 2,000u $41.71

Life Technologies LC5925 SeeBlue® Plus 2 Pre-Stained Standard - 500µl $134.79

Bio-Rad 223-9955 Semi-Micro Disposable Cuvettes, 1.5ml - 100pk $11.69

Promega V5111 Sequencing Grade Modified Trypsin - 100µg $84.80

Bio-Rad 161-0449 Silver Stain Plus Kit $199.75

Life Technologies LC6060 SimplyBlue™ SafeStain - 1L $129.02

New England Biolabs R0141S Sma I - 2,000u $41.71

Quality Biological 340-031-671 SOC Medium - 10 x 10ml $33.23

Life Technologies 25080094 Sodium Bicarbonate Soln, 7.5% (w/v) - 100ml $9.34

Quality Biological 351-036-101 Sodium Chloride, 5M - 500ml $10.06

Sigma-Aldrich S8636-100ML Sodium Pyruvate - 100ml $6.49

Life Technologies 11360070 Sodium Pyruvate Solution - 100ml $8.96

Fermentas 26634 Spectra™ Multicolor Broad Range Ladder - 50apps $110.10

New England Biolabs R3133S SpeI-HF – 500u $45.31

New England Biolabs R3182S SphI-HF - 500u $45.31

Life Technologies A1110501 StemPro Accutase Cell Dissociation Reagent - 100ml $37.60

New England Biolabs R0187S Stu I - 1,000u $41.71

New England Biolabs C2988J Subcloning Efficiency 5-alpha Comp cells- 6 x 0.4ml $46.75

Life Technologies 18265017 SUB Efficiency DH5 Alpha™ Comp Cells - 2ml $52.61

Sigma-Aldrich S5016-500G Sucrose ACS Reagent - 500g $13.95

Life Technologies 11904018 SuperScript™ 1st Strand System for RT-PCR $379.34

Life Technologies 18064014 SuperScript™ II Reverse Transcriptase - 10,000u $270.55

Life Technologies 18080044 SuperScript™ III - 10,000u $281.18

Life Technologies 18080051 SuperScript™ III First Strand Syntesis Kit $394.75

Supplier Catalog # Description Core Store Price

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Life Technologies 18080300 SuperScript™ III Cells Direct - 100rxns $718.25

Sigma-Aldrich S4438-100RXN SYBR Green Jumpstart Taq Readymix- 100rxns $105.51

Life Technologies S33102 SYBR Safe DNA Gel Stain – 400µl $59.00

Life Technologies 4309155 SYBR® Green PCR Master Mix (1x5ml) - 200rxns $278.38

New England Biolabs M0202S T4 DNA Ligase - 20,000u $46.03

New England Biolabs M0202T T4 DNA Ligase (2,000,000u/ml) - 20,000u $46.03

Life Technologies 15224017 T4 DNA Ligase - 100u $60.55

New England Biolabs M0202L T4 DNA Ligase - 100,000u $184.12

New England Biolabs M0203S T4 DNA Polymerase - 150u $46.03

New England Biolabs M0201S T4 Polynucleotide Kinase - 500u $40.28

Life Technologies 15558026 TAE Buffer (10X) - 4L $110.43

New England Biolabs M0273S Taq DNA Polymerase with Standard Taq Buffer- 400u $43.87

New England Biolabs M0267S Taq DNA Polymerase with ThermoPol Buffer – 400u $43.87

Life Technologies 10342020 Taq DNA Polymerase, Recombinant - 500u $78.86

Life Technologies 18038042 Taq DNA Polymerase - 500u $80.55

QIAGEN 201203 Taq DNA Polymerase - 250u $117.85

New England Biolabs M0267L Taq DNA Polymerase with ThermoPol Buffer - 2,000u $175.48

Life Technologies 10342046 Taq DNA Polymerase, Recombinant - 1500u (3x500u) $224.81

QIAGEN 201205 Taq DNA Polymerase - 1000u $428.90

Life Technologies 4440038 TaqMan Universal Master Mix II with UNG - 1pk $363.31

Life Technologies 4440040 TaqMan Universal Master Mix II, w/o UNG - 200rxns $340.76

Life Technologies 4352042 TaqMan® Fast Universal PCR Master Mix (2X) - 250rxns $227.38

Life Technologies 4369016 TaqMan® Gene Expression Master Mix(1x5ml) - 200rxns $348.00

Life Technologies 4304437 TaqMan® Universal PCR Master Mix(1x5ml) - 200rxns $411.71

Quality Biological 351-001-131 TBE Buffer (10X) – 1L $21.92

Corning Cellgro 46-012-CM TBS (10X) - 6 x 1L $67.65

Quality Biological 351-086-101 TBS pH 7.4 (10X) - 500ml $15.45

Quality Biological 351-086-101CS TBS 10x pH 7.4 - 10 x 500ml $155.44

Quality Biological 351-011-131 TE pH 8.0 - 1L $19.14

Bio-Rad 161-0800 Temed - 5ml $16.56

Bio-Rad 170-4085 Thick Blot Paper, 9.5x15.2 cm - 50sheets $49.00

Promega L1170 TNT® T7 Quick Coupled Transcript./Translation - 5rxns $502.23

Life Technologies K450001SC TOPO TA Cloning®, top 10 - 20 rxns $456.37

Life Technologies 10488085 TrackIt™ 1 Kb Plus DNA Ladder - 100apps $90.20

Sigma-Aldrich T9039-10PAK Tris Buffered Saline, ph 8.0 - 10/pkgs $36.26

Quality Biological 351-006-101 Tris-HCL 1M pH 7.5 - 500ml $13.43

Quality Biological 351-007-101 Tris-HCL 1M pH 8.0 - 500ml $13.43

Sigma-Aldrich T2194-1L Tris-HCL, 1M, pH 7.4 - 1L $35.88

Life Technologies LC3675 Trisgly SDS Transfer Buffer (25X) - 500ml $36.39

Sigma-Aldrich T9284-100ML Triton X 100 - 100ml $53.13

Sigma-Aldrich T6066-1KG Trizma® Base - 1kg $84.13

Sigma-Aldrich T1503-5KG Trizma® Base - 5kg $234.53

Life Technologies 15596026 TRIZOL® Reagent - 100ml $160.79

Life Technologies 15596018 TRIZOL® Reagent - 200ml $267.07

Corning Cellgro 25-900-CI Trypan Blue Solution, 0.4% - 1 x 100ml $10.70

Supplier Catalog # Description Core Store Price

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Life Technologies 15250061 Trypan Blue Stain - 100ml $14.35

Life Technologies 12604013 TrypLE™ Express - 100ml $14.99

Life Technologies 12605010 TrypLE™ Express w/Phenol Red - 100ml $15.24

Sigma-Aldrich T4049-100ML Trypsin-EDTA 0.25% - 100ml $9.75

Life Technologies 25200056 Trypsin-EDTA 0.25% (1X) - 100ml $10.54

Quality Biological 118-093-721 Trypsin-EDTA 0.25% - 4 x 100ml $28.90

Corning Cellgro 25-053-CI Trypsin EDTA (1X) .25% w/o CaMg - 6 x 100ml $33.83

Life Technologies 25200114 Trypsin-EDTA 0.25% - 20 x 100ml $192.56

Life Technologies 25300054 Trypsin-EDTA .05% (1X) - 100ml $6.81

Life Technologies 15400054 Trypsin, 0.5% (10X) - 100ml $27.72

Corning Cellgro 25-052-CI Trypsin-EDTA .05% - 6 x 100ml $33.83

Life Technologies 25300120 Trypsin-EDTA .05% - 20 x 100ml $125.28

Life Technologies AM1907 TURBO DNA-free Kit - 50rxns $96.04

Sigma-Aldrich P7949-100ML Tween 20 - 100ml $28.89

Life Technologies 16500500 UltraPure™ Agarose - 500g $338.26

New England Biolabs M0254S Vent DNA Polymerase - 200u $46.75

USB-Affymetrix 75650 200 RXN VeriQuest Probe qPCR Master Mix (2X) - 200rxns $252.54

USB-Affymetrix 75600 200 RXN VeriQuest SYBR Green qPCR Master Mix (2X) - 200rxns $240.81

USB-Affymetrix 75665 200 RXN VeriQuest SYBR Green qPCR Master Mix with Fluoresc $240.81

Quality Biological 118-162-131 Water, Cell Culture Grade (Ultra Pure) - 1L $15.90

Quality Biological 351-029-721 Water, Molecualr Biology Grade - 4 x 100ml $29.51

Quality Biological 351-029-131 Water, Molecular Biology Grade- 1L $11.19

Corning Cellgro 46-000-CI Water, Molecular Grade - 6 x 100ml $27.35

Quality Biological 351-161-671 Water, PCR Qualified Pure , 10 x 10ml $86.12

Promega A7640 Wizard® Plus Midipreps DNA Kit - 25preps $177.20

Promega A1460 Wizard® Plus SV Minipreps DNA Kit - 250preps $237.82

Corning Cellgro 46-101-RF X-Gal - 1g $152.19

Roche Applied Science 6366244001 X-tremeGENE HP DNA Transfection Reagent - .4ml $183.74

New England Biolabs R0145S Xba I - 3,000u $46.75

New England Biolabs R0145L Xba I - 15,000u $186.99

New England Biolabs R0146S Xho I - 5,000u $46.75

Agilent Technologies 200236 XL1-Blue SuperCompetent Cells - 5 x 0.2ml $183.74

Agilent Technologies 200315 XL10-Gold® Competent Cells - 10 x 100µl $217.15

New England Biolabs R0180S Xma I - 500u $45.31

Life Technologies R25001 Zeocin™ - 1g $192.93

Agilent Technologies Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Agilent Technologies 230132 BL21-Gold (DE3) Competent Cells - 5 x 0.2ml $179.10

Agilent Technologies 600153 Cloned Pfu DNA Polymerase - 100u $100.22

Agilent Technologies 500402 Dpn I - 200u $91.87

Agilent Technologies 200049 G418 Sterile Solution - 10ml 50mg/ml $72.38

Agilent Technologies 600675 Herculase® II Fusion DNA Polymerase - 40rxns $48.26

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 21

Agilent Technologies 600677 Herculase® II Fusion with dNTPs Combo - 200rxns $215.30

Agilent Technologies 600250 PfuTurbo® DNA Polymerase - 100u $103.94

Agilent Technologies 600252 PfuTurbo® DNA Polymerase - 500u $425.02

Agilent Technologies 600380 PfuUltra™ High-fidelity DNA Polymerase - 100u $116.00

Agilent Technologies 600670 PfuUltra™ II Fusion HS DNA Polymerase - 40rxns $96.51

Agilent Technologies 210518 QuikChange Lightning - 10rxns $234.78

Agilent Technologies 200521 QuikChange® II XL site-directed mutagenesis kit $244.06

Agilent Technologies 200236 XL1-Blue SuperCompetent Cells - 5 x 0.2ml $183.74

Agilent Technologies 200315 XL10-Gold® Competent Cells - 10 x 0.1ml $217.15

Bio-Rad Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Bio-Rad 170-8352 100 bp, EZ Load Molecular Ruler - 100lanes $112.00

Bio-Rad 170-6435 10X Tris Buffered Saline - 1L $37.03

Bio-Rad 161-0771 10X tris/glycine - 5L $85.75

Bio-Rad 161-0772 10X tris/glycine/sds - 5L $85.75

Bio-Rad 161-0156 30% Acryl/Bis Solution 29:1 - 500ml $55.54

Bio-Rad 161-0158 30% Acryl/Bis Solution 37.5:1 - 500ml $55.54

Bio-Rad 161-0144 40% Acryl/Bis Solution 19:1 - 500ml $66.26

Bio-Rad 161-0148 40% Acryl/Bis Solution 37.5:1 - 500ml $66.26

Bio-Rad 223-9441 96 - Well 0.2 ml PCR Plates - 25/pack $199.00

Bio-Rad 161-0700 Ammonium Persulfate - 10g $13.64

Bio-Rad 170-3965 Blot Paper, Extra Thick, 7.5 x 10 $52.62

Bio-Rad 170-6404 Blotting Grade Blocker-NFDM $42.87

Bio-Rad 170-5060 Clarity Western ECL Substrate - 200ml $119.85

Bio-Rad 170-5061 Clarity Western ECL Substrate - 500ml $228.98

Bio-Rad 345-0033 Criterion™ Gel Tris-HCI 4-20% 18 wells -1gel $15.00

Bio-Rad 345-0006 Criterion™ Gel, Tris-HCL 7.5%, 18 wells - 1gel $15.00

Bio-Rad 345-0034 Criterion™ Gel, Tris-HCl, 4-20% 26wells - 1gel $15.00

Bio-Rad 345-0010 Criterion™ Tris-HCL gels 10% 18-well comb - 30µl $15.00

Bio-Rad 165-2089 Gene Pulser® Cuvette, 0.1cm - 50pk $169.55

Bio-Rad 161-0718 Glycine - 1kg $50.00

Bio-Rad 162-0174 ImmunBlot PVDF Membrane - 10pk $79.90

Bio-Rad 162-0177 ImmunBlot PVDF, 26cm x 3.3m - 1roll $294.27

Bio-Rad 170-8880 iQ SYBR Green Supermix - 100 x 50µl $178.32

Bio-Rad 170-8882 iQ SYBR Green Supermix - 500 x 50µl $819.00

Bio-Rad 170-8841 iScript RT Supermix, 100rxns $465.00

Bio-Rad 170-8840 iScript RT Supermix, 25rxns $150.00

Bio-Rad 170-8890 iScript™ cDNA Synthesis Kit - 25rxns $135.00

Bio-Rad 170-8891 iScript™ cDNA Synthesis Kit - 100rxns $394.63

Bio-Rad 172-5122 iTaq Univeral SYBR Green SMX 10ml $498.89

Bio-Rad 172-5121 iTaq Univeral SYBR Green SMX 5ml $259.19

Bio-Rad 161-0324 Kaleidoscope™ Prestained Standards - 500µl $115.00

Bio-Rad 161-0737 Laemmli Sample Buffer - 30ml $13.64

Bio-Rad MSB-1001 Microseal B Seals - 100pk $153.00

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 22

Bio-Rad 170-4157 Midi PVDF Transfer pack - 10pk $194.00

Bio-Rad 456-1034 Mini Protean® TGX™ 10% 10well 50µl - 10gels $96.30

Bio-Rad 456-1033 Mini Protean® TGX™ 10%, 10-well 30µl -10gels $103.00

Bio-Rad 456-1043 Mini Protean® TGX™ 12%, 10-well 30µl - 10gels $103.00

Bio-Rad 456-1083 Mini Protean® TGX™ 4-15%, 10-well 30µl - 10gels $96.30

Bio-Rad 456-1086 Mini Protean® TGX™ 4-15%, 15-well 15µl - 10gels $96.30

Bio-Rad 456-1084 Mini Protean® TGX™ 4-15%, 10-well 50µl - 10gels $96.30

Bio-Rad 456-1093 Mini Protean® TGX™ 4-20%, 10-well 30µl - 10gels $96.30

Bio-Rad 456-1096 Mini Protean® TGX™ 4-20%,15-well 15µl - 10gels $96.30

Bio-Rad 456-1023 Mini Protean® TGX™ 7.5%, 10-well 30µl -10gels $103.00

Bio-Rad 170-3932 Mini Trans-Blot Filter Paper $44.82

Bio-Rad 162-0115 Nitrocellulose, 0.45,33cm x 3M - 1roll $249.45

Bio-Rad TCS-0803 Optical Flat 8-Cap Strips - 120strips $36.00

Bio-Rad 161-0373 Precision Plus All Blue Standards - 500µl, 50apps $94.52

Bio-Rad 161-0375 Precision Plus Protein Kaleidoscope Standard – 500µl $126.67

Bio-Rad 161-0374 Precision Plus Protein Standards - 500µl $116.93

Bio-Rad 161-0734 Premixed Buffers, 10x Tris/Glycine - 1L $26.31

Bio-Rad 161-0732 Premixed Running Buffer - 1L $26.31

Bio-Rad 161-0318 Prestained STDS, Broad Range - 500µl $97.44

Bio-Rad 500-0006 Protein Assay Dye Reagent - 450ml $109.13

Bio-Rad 732-6120 Quantum Prep™ Midiprep Kit - 20preps $174.42

Bio-Rad 223-9955 Semi-Micro Disposable Cuvettes, 1.5ml - 100pk $11.69

Bio-Rad 161-0449 Silver Stain Plus Kit $199.75

Bio-Rad 161-0800 Temed - 5ml $16.56

Bio-Rad 170-4085 Thick Blot Paper, 9.5x15.2 cm - 50sheets $49.00

Cell Signaling Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Cell Signaling 9272S Akt Antibody - 100µl $207.38

Cell Signaling 7076S Anti-mouse IgG, HRP-linked Antibody - 1ml $111.93

Cell Signaling 7074S Anti-rabbit IgG, HRP-linked Antibody - 1ml $111.93

Cell Signaling 9662S Caspase-3 Antibody - 100µl $207.38

Cell Signaling 9803S Cell Lysis Buffer (10X) - 15ml $51.19

Cell Signaling 9661S Cleaved Caspase-3 (Asp175) Antibody - 100µl $242.08

Cell Signaling 4060S Phospho-Akt (Ser473) (D9E) XP(tm) Rabbit mAb-100µl $268.11

Cell Signaling 9271S Phospho-Akt (Ser473) Antibody - 100µl $242.08

Cell Signaling 4370S Phospho-p44/42 MAPK (Erk1/2) Rabbit mAb - 200µl $268.11

Cell Signaling 9071S ProLong(R) Gold Antifade Reagent - 10ml $137.96

Cell Signaling 8961S ProLong(R) Gold Antifade Reagent with DAPI - 10ml $146.64

Cell Signaling 5872S Protease/Phosphatase Inhibitor Cocktail (100X) - 1ml $207.38

Cell Signaling 9806S Ripa Buffer (10x) - 15ml $51.19

Corning Cellgro Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Corning Cellgro 61-238-RH Ampicillin, Sodium Salt Powder - 10g $42.80

Corning Cellgro 30-100-RB Blasticidin S HCl, Powder - 50mg $92.25

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 23

Corning Cellgro 25-055-CV Distilled Water - 6 x 500ml $30.75

Corning Cellgro 10-014-CV DMEM - 1 gm/L glucose - 6 x 500ml $30.75

Corning Cellgro 10-013-CV DMEM - 4.5 gm/L glucose - 6 x 500ml $30.75

Corning Cellgro 10-092-CV DMEM/F-12 - 50/50 Mix - 6 x 500ml $49.20

Corning Cellgro 15-090-CV DMEM/F-12 - 50/50 Mix w/o L-Glutamine- 6 x 500ml $43.99

Corning Cellgro 25-950-CQC DMSO - 250ml $52.32

Corning Cellgro 21-031-CV DPBS (1X) w/o CaMg - 6 x 500ml $30.75

Corning Cellgro 46-034-CI EDTA .5 M , pH 8.0 - 100ml $14.27

Corning Cellgro 35-010-CV Fetal Bovine Serum - 500ml $117.71

Corning Cellgro 35-011-CV Fetal Bovine Serum, Heat Inactivated - 500ml $129.60

Corning Cellgro 61-234-RF G418 - 1g $65.40

Corning Cellgro 61-234-RG G418 - 5g $187.86

Corning Cellgro 30-234-CR G418 Sulfate Solution - 20ml $85.61

Corning Cellgro 10-080-CV Ham's F-12 w/L-Glut - 6 x 500ml $46.40

Corning Cellgro 21-021-CV HBSS (1X) w/o CaMg - 6 x 500ml $30.75

Corning Cellgro 21-022-CV HBSS w/o CaMg & Phenol Red - 6 x 500ml $30.75

Corning Cellgro 25-060-CI Hepes Buffer (1M) - 6 x 100ml $117.71

Corning Cellgro 30-240-CR Hygromycin B Solution - 1 x 20 ml $118.90

Corning Cellgro 46-102-RF IPTG - 1g $23.58

Corning Cellgro 25-800-CR ITS(insulin-Transferrin Selenium)100x Solution - 10ml $32.10

Corning Cellgro 25-005-CI L-Glutamine - 6 x 100ml $42.00

Corning Cellgro 25-072-CI Lymphocyte separation medium - 100ml $11.89

Corning Cellgro 10-050-CV McCoy's 5A w/L-Glut - 6 x 500ml $54.69

Corning Cellgro 10-010-CV MEM w/ Earle’s salts & L-Glutamine (1X) - 6 x 500ml $30.75

Corning Cellgro 21-040-CV PBS (1X) w/o CaMg - 6 x 500ml $30.75

Corning Cellgro 46-013-CM PBS (10X) pH 7.4 - 6 x 1L $73.72

Corning Cellgro 30-001-CI Penicillin Streptomycin - 6 x 100ml $38.05

Corning Cellgro 10-040-CV RPMI 1640 w/L-Glutamine - 6 x 500ml $30.75

Corning Cellgro 46-012-CM TBS (10X) - 6 x 1L $67.65

Corning Cellgro 25-900-CI Trypan Blue Solution, 0.4% - 1 x 100ml $10.70

Corning Cellgro 25-053-CI Trypsin EDTA (1X) .25% w/o CaMg - 6 x 100ml $33.83

Corning Cellgro 25-052-CI Trypsin-EDTA .05% - 6 x 100ml $33.83

Corning Cellgro 46-000-CI Water, Molecular Grade - 6 x 100ml $27.35

Corning Cellgro 46-101-RF X-Gal - 1 x 1g $152.19

Faster Better Media Some special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Faster Better Media SB20-4 20X SB - 4L cube $102.00

Faster Better Media SB20-1 20X SB low conductive medium -1L $33.00

Faster Better Media SB5N-8 5X SB loading medium - 8 tubes, 1.2ml per tube $23.00

GE Healthcare Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

GE-Healthcare RPN2109 ECL Western Blotting Detection Reagents $103.86

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 24

GE-Healthcare RPN2232 ECL Prime Western Blotting rgt, For 1000 cm2 $160.08

GE-Healthcare RPN2106 ECL Western Blotting Detection Rgts- 2 x 250ml $226.54

GE-Healthcare 17-1440-03 Ficoll-Paque™ PLUS - 6 x 500ml $565.00

GE-Healthcare 17-1440-02 Ficoll-Paque™ PLUS- 6 x 100ml $164.66

GE-Healthcare 17-0756-01 Glutathione Sepharose™ - 10ml $261.00

GE-Healthcare 28-9068-35 Hyperfilm ECL 5x7in - 50 Sheets $199.52

GE-Healthcare 28-9068-39 Hyperfilm ECL 8x10in - 100Sheets $393.47

GE-Healthcare 28-9068-38 Hyperfilm ECL 8x10in - 50Sheets $235.71

GE-Healthcare NA931-1ML Mouse IgG, HRP-Linked Whole Ab (sheep) -1ml $254.68

GE-Healthcare 17-0891-01 Percoll® (Sterile) - 1L $380.00

GE-Healthcare 17-0618-01 Protein G Sepharose™ 4 Fast Flow - 5ml $509.00

GE-Healthcare NA934-1ML Rabbit IgG, HRP-linked Ab (donkey) - 1ml $200.05

GE-Healthcare RPN800E Rainbow™ Molecular Weight Marker IMPROVED - 250µl $108.46

Hyclone Some special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Hyclone SH30071.03 Fetal Bovine Serum, Characterized - 500ml $193.43

Hyclone SH30070.03 Fetal Bovine Serum, Defined - 500ml $273.47

Life Technologies Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Life Technologies 10787018 1 kb Plus DNA Ladder - 250µg $123.26

Life Technologies 15628019 100 bp DNA Ladder - 50µg $75.39

Life Technologies 21985023 2-Mercaptoethanol, 55mM - 50ml $32.64

Life Technologies 4346907 96-well Fast Thermal Cycling Plates, 10 $26.39

Life Technologies R96025 Anti-V5 antibody - 50µl $211.82

Life Technologies 15240062 Antibiotic Antimycotic (100X) - 100ml $31.77

Life Technologies 17504044 B-27 Serum Free Supplement (50x) - 10ml $81.84

Life Technologies 12587010 B-27 Supplement w/o Vitamin A (50X) - 10ml $99.17

Life Technologies 10748010 BENCHMARK™ Prestained Protein Ladder - 500u $171.38

Life Technologies 13256029 bFGF, Human, Recombinant - 10µg $157.90

Life Technologies R21001 Blasticidin - 50mg $153.63

Life Technologies 11789020 BP clonase II enzyme mix - 20rxns $185.82

Life Technologies A1048301 Collagen I, Rat Tail - 20ml $156.94

Life Technologies C10228 Countess Chamber Slides-50slides $86.50

Life Technologies 15230204 Distilled Water - 10 x 500ml $75.97

Life Technologies 15230162 Distilled Water - 500ml $7.99

Life Technologies 10977015 Distilled Water (Ultrapure) - 500ml $27.92

Life Technologies 11965118 DMEM (1X), high glucose - 10 x 500ml $74.15

Life Technologies 11965092 DMEM (1X), high glucose - 500ml $7.78

Life Technologies 11995073 DMEM (1X), high glucose w/sodium pyruvate - 10x500ml $76.33

Life Technologies 11995065 DMEM (1X), high glucose w/sodium pyruvate - 500ml $10.54

Life Technologies 11885092 DMEM (1X), low glucose - 10 x 500ml $91.59

Life Technologies 11885084 DMEM (1X), low glucose - 500ml $7.78

Life Technologies 11330057 DMEM/F-12, liquid (1:1) - 10 x 500ml $138.32

Life Technologies 11330032 DMEM/F-12, liquid (1:1) - 500ml $30.04

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 25

Life Technologies AM1906 DNA-free™ - 50rxns $98.21

Life Technologies 18068015 DNase I - Amplification Grade - 100u $95.82

Life Technologies 18427013 dNTP Mix 10mM - 100µl $30.30

Life Technologies 18427088 dNTP Mix 10mM - 1ml $185.14

Life Technologies 10297018 dNTP Set 100mM - 4 x 25µmol $189.28

Life Technologies 14200075 DPBS (10X) - 500ml $32.54

Life Technologies 14190250 DPBS (1X) w/o CaMg - 10 x 500ml $70.53

Life Technologies 14190144 DPBS (1X) w/o CaMg - 500ml $7.88

Life Technologies 4385612 Fast SYBR® Green Master Mix - 1 x 5ml $308.69

Life Technologies 26140079 Fetal Bovine Serum U.S., Qualified - 500ml $183.56

Life Technologies 16000044 Fetal Bovine Serum, Certified - 500ml $239.60

Life Technologies 16140071 Fetal Bovine Serum, Qual, Heat Inactivated - 500ml $187.17

Life Technologies 11791020 GATEWAY®LR clonase II enzyme mix - 20rxns $214.00

Life Technologies A1413302 Geltrex LDEV-Free hESC-qualified - 5ml $193.52

Life Technologies A1413202 Geltrex LDEV-free RGF - 5ml $174.27

Life Technologies N8080007 GeneAmp® dNTP's - (4 x 320µl of 10mM soln) $45.17

Life Technologies 10131035 Geneticin® - 20ml $117.46

Life Technologies 11811023 Geneticin® Selective Antibiotic, powder - 1g $77.34

Life Technologies 11811031 Geneticin® Selective Antibiotic, powder - 5g $234.75

Life Technologies 35050061 GlutaMAX-I Supplement - 100ml $25.74

Life Technologies 14170161 HBSS (1X) w/o CaMg - 10 x 500ml $74.24

Life Technologies 14170112 HBSS (1X) w/o CaMg - 500ml $8.31

Life Technologies 14175103 HBSS (1X) w/o phenol red - 10 x 500ml $77.95

Life Technologies 14175095 HBSS (1X) w/o phenol red - 500ml $8.51

Life Technologies 15630080 Hepes Buffer Solution (1M) - 100ml $39.53

Life Technologies 4368814 High Capacity cDNA Kit - 200 Rxns $188.79

Life Technologies IB301031 iBlot® Transfer Stack, Regular 3x10sets $444.81

Life Technologies IB301001 iBlot® Transfer Stack, Regular-10 set $177.16

Life Technologies 12440061 IMDM (1X) - 10 x 500ml $179.19

Life Technologies 12440053 IMDM (1X) - 500ml $24.24

Life Technologies 12585014 Insulin, Human Recombinant, Zinc Solution - 5ml $37.55

Life Technologies LC2005 Invitrolon PVDF/Filter - 20pk $185.82

Life Technologies 15529019 IPTG - 1g $54.22

Life Technologies 17005042 Keratinocyte-SFM with supplements - 500ml $82.80

Life Technologies 10829018 Knockout™ DMEM - 500ml $26.09

Life Technologies 10828028 Knockout™ Serum - 500ml $266.70

Life Technologies 25030081 L-Glutamine 200mM (100X) - 100ml $16.29

Life Technologies 23017015 Laminin, Mouse, Natural - 1mg $112.65

Life Technologies 11415064 Leibovitz's L-15 Medium with/phenol (1X) - 500ml $30.04

Life Technologies 18263012 LIBRARY Efficiency™ DH5Alpha™ Comp Cells - 1ml $129.02

Life Technologies 11668027 Lipofectamine™ 2000 Transfection Reagent - .75ml $274.92

Life Technologies 11668019 Lipofectamine™ 2000 Transfection Reagent - 1.5ml $508.36

Life Technologies 15338100 Lipofectamine™ LTX and Plus Reagent - 1ml $482.56

Life Technologies 13778075 Lipofectamine™ RNAiMAX - 0.75ml $367.41

Life Technologies LC5602 Magic Mark XP Standard-250µl $255.14

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 26

Life Technologies 18258012 MAX Efficiency DH5Alpha™ Competent Cells - 1ml $171.27

Life Technologies 16600108 McCoy's 5A Medium (1X) - 10 x 500ml $139.94

Life Technologies 16600082 McCoy's 5A Medium (1X) - 500ml $23.06

Life Technologies 11140050 MEM NEAA (100X) 10mM - 100ml $15.98

Life Technologies 11095098 MEM w/ L-glutamine (1X) - 10 x 500ml $71.97

Life Technologies 11095080 MEM w/ L-glutamine (1X) - 500ml $8.54

Life Technologies 4346906 MicroAmp® Fast Optical 96-Well Plate - 20plates $63.52

Life Technologies N8010560 MicroAmp® Optical 96-Well Reaction Plate-10/bx $49.58

Life Technologies 4311971 MicroAmp® Optical Adhesive Film - 100covers $165.13

Life Technologies 4306737 MicroAmp™ Optical 96 Well Plate - 20plates $101.04

Life Technologies AM1561 mirVana™ miRNA Isolation Kit - 40purifications $237.81

Life Technologies 4311235 MultiScribe™ Reverse Transcriptase - 100µl $70.91

Life Technologies N8080018 MuLV Reverse Transcriptase - 5,000u $58.95

Life Technologies 17502048 N-2 Supplement - 5ml $67.40

Life Technologies 21103049 Neurobasal-SFM - 500ml $55.84

Life Technologies LC2000 Nitrocellulose and filter paper (.2um;8.3x7.5cm) $148.27

Life Technologies LC5800 Novex Sharp Pre-stained Protein Standard- 2x250µl $141.53

Life Technologies NP0321BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 10 well-10gels $136.72

Life Technologies NP0322BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 12 well-10 gels $136.72

Life Technologies NP0323BOX NuPAGE® 4-12% Bis-Tris Gel 1.0 mm, 15 well-10gels $136.72

Life Technologies NP0335BOX NuPAGE® 4-12% Bis-Tris Gel 1.5 mm, 10 well-10gels $136.72

Life Technologies NP0005 NuPAGE® Antioxidant - 15ml $24.45

Life Technologies NP0007 NuPAGE® LDS Sample Buffer (4X) - 10ml $12.76

Life Technologies NP0002 NuPAGE® MES SDS Running Buffer(B-T Gels 20X) - 500ml $68.85

Life Technologies NP0001 NuPAGE® MOPS SDS Running Buffer(B-T Gels 20X) - 500ml $68.85

Life Technologies NP0004 NuPAGE® Sample Reducing Agent (10X) - 250µl $12.76

Life Technologies NP0006 NuPAGE® Transfer Buffer (20X) - 125ml $21.63

Life Technologies NP00061 NuPAGE® Transfer Buffer 20X - 1L $150.20

Life Technologies 18418012 Oligo(dT)12-18 Primer - 25µg $77.02

Life Technologies C737303 One Shot® Stabl 3 Comp E coli $360.41

Life Technologies C404003 One Shot® TOP10 - 20rxn $331.89

Life Technologies 31985088 Opti-MEM I - 10 x 500ml $348.53

Life Technologies 31985070 Opti-MEM I - 500ml $40.05

Life Technologies 10010049 PBS (1X) w/o CaMg, liquid - 10 x 500ml $72.38

Life Technologies 10010023 PBS (1X) w/o CaMg, liquid - 500ml $7.46

Life Technologies 15140122 Penicillin Streptomycin - 100ml $18.91

Life Technologies 15140163 Penicillin Streptomycin - 20 x 100ml $213.57

Life Technologies 15593031 Phenol : Chloroform:(25:24:1,v/v) - 100ml $98.21

Life Technologies 11306016 PLATINUM® PCR Super Mix - 100rxns $105.56

Life Technologies 11708013 PLATINUM® PFX DNA polymerase - 100u $126.13

Life Technologies 10966018 PLATINUM® Taq DNA Polymerase - 100u $95.32

Life Technologies 10966034 PLATINUM® Taq DNA Polymerase - 500u $362.06

Life Technologies 11304011 PLATINUM® Taq DNA Polymerase High Fidelity - 100u $158.86

Life Technologies 4367659 Power SYBR® Green PCR Master Mix (1 x 5ml)-200rxns $275.62

Life Technologies P36930 ProLong Gold Antifade Reagent - 10ml $136.72

Life Technologies P36931 Prolong Gold Antifade Reagent w/DAPI - 10ml $159.00

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 27

Life Technologies 25530015 Proteinase K - 100 mg $92.85

Life Technologies K210014 PureLink HiPure Plasmid Filter Midiprep Kit-25preps $225.28

Life Technologies K210016 PureLink™ HiPure Filter Maxi - 10preps $187.18

Life Technologies K210012 PureLink™ Quick Gel Extraction Kit-50preps $76.20

Life Technologies K210011 PureLink™ Quick Plasmid Miniprep Kit - 250preps $264.21

Life Technologies K210010 PureLink™ Quick Plasmid Miniprep Kit - 50preps $59.64

Life Technologies N8080127 Random Hexamers (50µm) - 1kit $62.37

Life Technologies 48190011 Random Primers - 9 A260u $77.02

Life Technologies 12648010 Recovery Cell Culture Medium - 50ml $110.72

Life Technologies 18021071 Ribonuclease H - 120u $286.76

Life Technologies N8080119 RNase Inhibitor - 2,000u $60.24

Life Technologies 10777019 RNaseOut™ - 5,000u $133.91

Life Technologies 11875119 RPMI 1640 (1X) w/ L-Glut - 10 x 500ml $74.15

Life Technologies 11875093 RPMI 1640 (1X) w/ L-Glut - 500ml $7.78

Life Technologies 11875135 RPMI 1640 (1X) w/ L-Glut - 6 x 1000ml $75.59

Life Technologies 61870036 RPMI 1640 w/ Glutamax - 500ml $13.78

Life Technologies 15544034 S.O.C. Medium - 10 x 10ml $86.42

Life Technologies LC5925 SeeBlue® Plus 2 Pre-Stained Standard - 500µl $134.79

Life Technologies LC6060 SimplyBlue™ SafeStain - 1L $129.02

Life Technologies 25080094 Sodium Bicarbonate Soln, 7.5% (w/v) - 100ml $9.34

Life Technologies 11360070 Sodium Pyruvate Solution - 100ml $8.96

Life Technologies A1110501 StemPro Accutase Cell Dissociation Reagent - 100ml $37.60

Life Technologies 18265017 SUB Efficiency DH5 Alpha™ Competent Cells - 2ml $52.61

Life Technologies 11904018 SuperScript™ 1st Strand System for RT-PCR $379.34

Life Technologies 18064014 SuperScript™ II Reverse Transcriptase - 10,000u $270.55

Life Technologies 18080044 SuperScript™ III - 10,000u $281.18

Life Technologies 18080300 SuperScript™ III Cells Direct - 100rxns $718.25

Life Technologies 18080051 SuperScript™ III First Strand Syntesis Kit $394.75

Life Technologies S33102 SYBR Safe DNA Gel Stain - 400µl $59.00

Life Technologies 4309155 SYBR® Green PCR Master Mix (1x5ml) - 200rxns $278.38

Life Technologies 15224017 T4 DNA Ligase - 100u $60.55

Life Technologies 15558026 TAE Buffer (10X) - 4L $110.43

Life Technologies 18038042 Taq DNA Polymerase - 500u $80.55

Life Technologies 10342046 Taq DNA Polymerase, Recombinant - 1500u (3x500u) $224.81

Life Technologies 10342020 Taq DNA Polymerase, Recombinant - 500u $78.86

Life Technologies 4440038 TaqMan Universal Master Mix II with UNG - 1pk $363.31

Life Technologies 4440040 TaqMan Universal Master Mix II, no UNG - 200rxns $340.76

Life Technologies 4352042 TaqMan® Fast Universal PCR Master Mix (2X) - 250rxns $227.38

Life Technologies 4369016 TaqMan® Gene Expression Master Mix(1 x 5ml) - 200rxns $348.00

Life Technologies 4304437 TaqMan® Universal PCR Master Mix(1 x 5ml) - 200rxns $411.71

Life Technologies K450001SC TOPO TA Cloning®, top 10 - 20rxns $456.37

Life Technologies 10488085 TrackIt™ 1 Kb Plus DNA Ladder - 100apps $90.20

Life Technologies LC3675 Trisgly SDS Transfer Buffer (25X) - 500ml $36.39

Life Technologies 15596026 TRIZOL® Reagent - 100ml $160.79

Life Technologies 15596018 TRIZOL® Reagent - 200ml $267.07

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 28

Life Technologies 15250061 Trypan Blue Stain - 100ml $14.35

Life Technologies 12604013 TrypLE™ Express - 100ml $14.99

Life Technologies 12605010 TrypLE™ Express w/Phenol Red - 100ml $15.24

Life Technologies 25300120 Trypsin-EDTA .05% - 20 x 100ml $125.28

Life Technologies 25300054 Trypsin-EDTA .05% (1X) - 100ml $6.81

Life Technologies 25200114 Trypsin-EDTA 0.25% - 20 x 100ml $192.56

Life Technologies 25200056 Trypsin-EDTA 0.25% (1X) - 100ml $10.54

Life Technologies 15400054 Trypsin, 0.5% (10X) - 100ml $27.72

Life Technologies AM1907 TURBO DNA-free Kit - 50rxns $96.04

Life Technologies 16500500 UltraPure™ Agarose - 500g $338.26

Life Technologies R25001 Zeocin™ - 1g $192.93

New England Biolabs Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

New England Biolabs N3232S 1 kb DNA Ladder - 100µg $36.68

New England Biolabs N3232L 1 kb DNA Ladder - 500µg $146.72

New England Biolabs N3231L 100 bp DNA Ladder - 250µg $155.35

New England Biolabs N3231S 100 bp DNA Ladder - 50µg $38.84

New England Biolabs N0467S 100 bp DNA Ladder, Quick Load - 125 gel lanes $57.54

New England Biolabs N3200S 2-Log DNA Ladder - 100µg $38.84

New England Biolabs N3200L 2-Log DNA Ladder - 500µg $155.35

New England Biolabs N3236S 50 bp DNA Ladder - 100µg $44.59

New England Biolabs R0117S Aat II - 500u $40.99

New England Biolabs R3552S AgeI-HF - 300u $48.19

New England Biolabs M0290S Alkaline Phosphatase,Calf Intest(CIP)-1,000u $49.62

New England Biolabs M0289S Antarctic Phosphatase - 1,000u $44.59

New England Biolabs R0114S Apa I - 5,000u $46.75

New England Biolabs R0558S Asc I - 500u $45.31

New England Biolabs P0756S ATP - (Adenosine-5 Triphosphate) 10 mM - 1ml $21.58

New England Biolabs R0174S Avr II - 100u $46.75

New England Biolabs R3136S BamH I - HF - 5,000u $39.56

New England Biolabs R0144S Bgl II - 2,000u $39.56

New England Biolabs C2530H BL21 Competent E. coli - 20 x 0.05ml/tube $128.74

New England Biolabs M0367S Blunt/TA Ligase Master Mix - 50rxns $68.32

New England Biolabs R0197S Cla I - 1,000u $43.15

New England Biolabs P7711S ColorPlus Prestained Protein Ladder,Broad - 100lanes $73.36

New England Biolabs P7709V ColorPlus Prestained Protein Marker $51.78

New England Biolabs M0226S CpG Methylase (Sss I Methylase) - 100u $46.75

New England Biolabs C2925H dam-/dcm- Competent E.coli - 20 transformations $163.26

New England Biolabs M0210S DNA Polymerase I, Klenow Fragment - 200u $42.43

New England Biolabs M0303S DNase I (RNase-Free) - 1,000u $47.47

New England Biolabs N0447S dNTP Mix - 8µmol ea. $40.28

New England Biolabs N0446S dNTP Set - 25µmol ea. $112.20

New England Biolabs R0176S Dpn I - 1,000u $43.15

New England Biolabs R3101S EcoR I HF - 10,000u $39.56

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 29

New England Biolabs R3195S EcoR V HF - 4000u $39.56

New England Biolabs R3195L EcoR V-HF - 20,000u $158.22

New England Biolabs B7021S Gel Loading Dye Blue (6x) - 6ml $31.64

New England Biolabs E2611S Gibson Assembly Master Mix $112.91

New England Biolabs R3104S Hind III-HF - 10,000u $39.56

New England Biolabs M0370S Instant Sticky-end Ligase Master Mix - 50 rxns $68.32

New England Biolabs R3142S Kpn I HF - 4,000u $43.15

New England Biolabs M0368L M-MuLV Reverse Transcriptase - 10,000u $117.95

New England Biolabs M0368S M-MuLV Reverse Transcriptase - 4,000u $58.97

New England Biolabs R3589S Mfe I - HF - 500u $48.91

New England Biolabs R0198S Mlu I - 1,000u $43.15

New England Biolabs R3193S NcoI - HF - 1,000u $41.71

New England Biolabs R0111S Nde I - 4,000u $43.15

New England Biolabs C2987H NEB 5-Alpha Competent Cells - 20 x .05ml $132.33

New England Biolabs C2992H NEB 5-alpha Competent E.coli - 20 transformations $132.33

New England Biolabs C2987I NEB 5-alpha Competent E.coli - 6 X 0.2 ml $103.56

New England Biolabs C2989K NEB 5-alpha Electrocompetent e. coli - 6 x .1ml $122.98

New England Biolabs C2984H NEB Turbo Competent E.coli - 20 x .05ml/tube $174.05

New England Biolabs R3131S NheI-HF - 1,000u $45.31

New England Biolabs R3189L Not I -HF - 2500u $195.62

New England Biolabs R3189S Not I-HF - 500u $48.91

New England Biolabs R0127S Nsi I - 1,000u $43.15

New England Biolabs M0480L OneTaq DNA Polymerase - 1000u $115.07

New England Biolabs M0480S OneTaq DNA Polymerase - 200u $28.77

New England Biolabs M0481L OneTaq Hot Start DNA Polymerase - 1000u $238.77

New England Biolabs R0547L Pac I - 1,250u $184.12

New England Biolabs R0547S Pac I - 250u $46.03

New England Biolabs M0530S Phusion High-Fidelity DNA Polymerase - 100u $75.52

New England Biolabs M0530L Phusion High-Fidelity DNA Polymerase - 500u $302.06

New England Biolabs M0531S Phusion High-Fidelity PCR Master Mix -100rxns $122.26

New England Biolabs M0531L Phusion High-Fidelity PCR Master Mix w/ HF Buffer $489.06

New England Biolabs R0560S Pme I - 500u $48.91

New England Biolabs P7703S Protein Ladder - (10-250 kDa) $53.94

New England Biolabs P7702S Protein Marker, Broad Range - 150 lanes $53.22

New England Biolabs R3140S Pst l-HF -10,000u $43.15

New England Biolabs R3150S Pvul-HF - 500u $48.19

New England Biolabs M0492S Q5 High-Fidelity 2X Master Mix - 100rxns $120.11

New England Biolabs M0491S Q5 High-Fidelity DNA Polymerase - 100u $74.08

New England Biolabs M0494S Q5 Hot Start High-Fidelity 2X Master Mix - 100rxns $140.24

New England Biolabs M0493S Q5 Hot Start High-Fidelity DNA Polymerase - 100u $89.90

New England Biolabs E0554S Q5 Site-Directed Mutagenesis Kit - 10rxns $129.46

New England Biolabs E0542S Quick Blunting™ Quick Ligation Kit - 20rxns $110.76

New England Biolabs M2200S Quick Ligation Kit - 30rxns $69.76

New England Biolabs M0297S RNase H - 250u $46.03

New England Biolabs M0307L RNase Inhibitor - 10,000u $215.76

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 30

New England Biolabs M0307S RNase Inhibitor - 2,000u $53.94

New England Biolabs R3156S Sac I HF - 2000u $39.56

New England Biolabs R0157S Sac II - 2,000u $41.71

New England Biolabs R3138S Sal I - HF - 2,000u $41.71

New England Biolabs R0141S Sma I - 2,000u $41.71

New England Biolabs R3133S SpeI-HF - 500u $45.31

New England Biolabs R3182S SphI-HF - 500u $45.31

New England Biolabs R0187S Stu I - 1,000u $41.71

New England Biolabs C2988J Subcloning Efficiency 5-alpha Comp cells- 6 x0.4ml $46.75

New England Biolabs M0202L T4 DNA Ligase - 100,000u $184.12

New England Biolabs M0202S T4 DNA Ligase - 20,000u $46.03

New England Biolabs M0202T T4 DNA Ligase (2,000,000u/ml) - 20,000u $46.03

New England Biolabs M0203S T4 DNA Polymerase - 150u $46.03

New England Biolabs M0201S T4 Polynucleotide Kinase - 500u $40.28

New England Biolabs M0273S Taq DNA Polymerase with Standard Taq Buffer- 400u $43.87

New England Biolabs M0267L Taq DNA Polymerase with ThermoPol Buffer - 2,000u $175.48

New England Biolabs M0267S Taq DNA Polymerase with ThermoPol Buffer - 400u $43.87

New England Biolabs M0254S Vent DNA Polymerase - 200u $46.75

New England Biolabs R0145L Xba I - 15,000u $186.99

New England Biolabs R0145S Xba I - 3,000u $46.75

New England Biolabs R0146S Xho I - 5,000u $46.75

New England Biolabs R0180S Xma I - 500u $45.31

Promega Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Promega G2101 100 bp DNA Ladder - 250µl (50 lanes) $100.92

Promega G1881 Blue/Orange Loading Dye, 6X (3X1ml) - 3ml $33.06

Promega G8091 Caspase-Glo® 3/7 Assay - 10ml $409.19

Promega G3580 CellTiter 96® AQueous One Assay - 1000assays $276.54

Promega G7571 CellTiter-Glo® Luminescent Cell Viability Assay $446.37

Promega U1515 dNTP Mix - 1,000µl $54.81

Promega E2920 Dual-Glo(TM) Luciferase Assay System - 10ml $201.00

Promega E1910 Dual-Luciferase® Reporter Assay System - 100 assay $193.07

Promega E1960 Dual-Luciferase® Reporter Assay System - 10pk $1,233.36

Promega E2693 FuGENE® 6 Transfection Reagent - .5ml $219.00

Promega E2691 FuGENE® 6 Transfection Reagent - 1ml $380.00

Promega E2311 FuGENE® HD Transfection Reagent - 1ml $449.00

Promega A5003 GoScript™ Reverse Transcriptase - 100rxns $107.82

Promega M7123 GoTaq Green Master Mix 1000rxns $283.50

Promega M3008 GoTaq® DNA Polymerase - 2,500u $467.48

Promega M3005 GoTaq® DNA Polymerase - 500u $105.56

Promega M8291 GoTaq® Flexi DNA Polymerase - 100u $24.13

Promega M8295 GoTaq® Flexi DNA Polymerase - 500u $105.56

Promega M7122 GoTaq® Green Master Mix - 100rxns $32.42

Promega L2001 JM109 Competent Cells, >108cfu/µg - 1ml (5 x 200µl) $124.61

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 31

Promega E1501 Luciferase Assay Reagent - 10pk $595.96

Promega E1500 Luciferase Assay System - 100assays $88.16

Promega M1701 M-MLV Reverse Transcriptase - 10,000u $48.26

Promega P1193 Nuclease-Free Water - 50ml (2 x 25ml) $44.37

Promega C1101 Oligo(dT)15 Primer - 20µg $56.20

Promega M7502 PCR Master Mix - 100rxns $64.09

Promega A1360 pGEM®-T Easy Vector System I - 20rxns $160.72

Promega C1181 Random Primers - 20µg $36.48

Promega N2511 Recombinant RNasin® Ribonuclease Inhibitor-2,500u $100.04

Promega N2115 RNasin® Ribonuclease Inhibitor - 10,000u $436.77

Promega N2111 RNasin® Ribonuclease Inhibitor - 2,500u $177.75

Promega V5111 Sequencing Grade Modified Trypsin - 100µg $84.80

Promega L1170 TNT® T7 Quick Coupled Transcript./Translation - 5rxns $502.23

Promega A7640 Wizard® Plus Midipreps DNA Kit - 25preps $177.20

Promega A1460 Wizard® Plus SV Minipreps DNA Kit - 250preps $237.82

QIAGEN Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

QIAGEN 69506 DNeasy Blood & Tissue Kit - 250 $642.39

QIAGEN 69504 DNeasy Blood & Tissue Kit - 50 $149.73

QIAGEN 201901 dNTP Mix PCR Grade - 800µl $156.49

QIAGEN 301425 Effectene Transfection Reagent - 1ml $287.87

QIAGEN 12362 EndoFree Plasmid Maxi Kit - (10) $310.09

QIAGEN 12662 HiSpeed Plasmid Maxi Kit (10) $256.96

QIAGEN 12663 HiSpeed Plasmid Maxi Kit (25) $619.21

QIAGEN 12643 HiSpeed Plasmid Midi Kit (25) $309.12

QIAGEN 203205 HotStarTaq DNA Polymerase (1000u) $510.05

QIAGEN 217004 miRNeasy Mini Kit (50) $327.47

QIAGEN 30210 Ni-NTA Agarose (25 ml) $268.55

QIAGEN 129115 Nuclease-Free Water - 1L $46.08

QIAGEN 205111 Omniscript RT Kit (50) $235.70

QIAGEN 301107 PolyFect Transfection Reagent (4 x 1 ml) $522.61

QIAGEN 19131 Proteinase K - 2 ml $81.43

QIAGEN 51104 QIAamp DNA Blood Mini Kit (50) $137.17

QIAGEN 51304 QIAamp DNA Mini Kit (50) $151.66

QIAGEN 20021 QIAEX II Gel Extraction Kit (150) $162.29

QIAGEN 12162 QIAGEN Plasmid Maxi Kit (10) $216.38

QIAGEN 12163 QIAGEN Plasmid Maxi Kit (25) $512.95

QIAGEN 12143 QIAGEN Plasmid Midi Kit (25) $250.19

QIAGEN 12123 QIAGEN Plasmid Mini Kit (25) $188.37

QIAGEN 27106 QIAprep Spin Miniprep Kit (250) $354.52

QIAGEN 27104 QIAprep Spin Miniprep Kit (50) $84.24

QIAGEN 28706 QIAquick Gel Extraction Kit (250) $496.52

QIAGEN 28704 QIAquick Gel Extraction Kit (50) $104.33

QIAGEN 28106 QIAquick PCR Purification Kit (250) $496.52

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 32

QIAGEN 28104 QIAquick PCR Purification Kit (50) $104.33

QIAGEN 79656 QIAshredder (250) $322.64

QIAGEN 79654 QIAshredder (50) $75.06

QIAGEN 204443 QuantiTect Probe RT-PCR Kit (200) $600.85

QIAGEN 205313 QuantiTect Rev. Transcription Kit (200) $1,068.40

QIAGEN 205311 QuantiTect Rev. Transcription Kit (50) $313.95

QIAGEN 204143 QuantiTect SYBR Green PCR Kit (200) $411.52

QIAGEN 76104 RNAlater RNA Stabilization Reagent $70.61

QIAGEN 19101 RNase A - 17,500u $184.51

QIAGEN 79254 RNase-Free DNase Set (50) $91.09

QIAGEN 74804 RNeasy Lipid Tissue Mini Kit (50) $341.96

QIAGEN 74106 RNeasy Mini Kit (250) $1,171.76

QIAGEN 74104 RNeasy Mini Kit (50) $266.62

QIAGEN 74134 RNeasy Plus Mini Kit (50) $300.43

QIAGEN 330401 RT2 First Strand cDNA Kit - 12rxns (20µl each) $229.91

QIAGEN 201205 Taq DNA Polymerase - 1000u $428.90

QIAGEN 201203 Taq DNA Polymerase - 250u $117.85

Quality Biological Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Quality Biological 351-059-101 10X RNA Gel Buffer (MOPS) - 500ml $21.54

Quality Biological 118-156-721 ACK Lysing Buffer - 4 x 100ml $43.98

Quality Biological 118-156-101 ACK Lysing Buffer - 500ml $27.05

Quality Biological 118-257-711 Cell Freezing Medium B - 4 x 50ml $80.59

Quality Biological 351-068-131CS DEPC Treated Water - 10 x 1L $201.68

Quality Biological 351-068-131 DEPC Treated Water - 1L $20.11

Quality Biological 351-068-721 DEPC Treated Water - 4 x 100ml $31.24

Quality Biological 351-028-661 DNA Gel Loading Solution (5X) - 5 x 10ml $47.73

Quality Biological 114-059-101 DPBS with /CaMg - 500ml $8.10

Quality Biological 112-018-101 E-MEM w/L-Glutamine - 500ml $8.09

Quality Biological 112-018-101CS EMEM w/ L-Glutamine – 10 x 500ml $84.05

Quality Biological 120-098-661 Gentamicin,50mg/ml - 5 x 10ml $106.27

Quality Biological 114-062-101 HBSS w/o phenol red & CaMg - 500ml $9.89

Quality Biological 118-089-721 Hepes Buffer Soln (1M) - 4 x 100ml $93.26

Quality Biological 112-035-101 IMDM - 500ml $17.61

Quality Biological 118-084-721 L-Glutamine 200mM, - 4 x 100ml $46.43

Quality Biological 340-147-231 LB Agar Plates w/50µg/ml kanamycin - 10ea x 25ml $22.59

Quality Biological 340-108-231 LB Agar Plates,100 µg/ml Ampicillin(25ml fill) - 10ea $23.77

Quality Biological 340-107-231 LB Agar Plates w/50mcg Ampicillin (25ml fill) - 10ea $23.66

Quality Biological 340-004-101CS LB Broth (Lauria Bertani), Case 10x500ml $79.94

Quality Biological 340-004-101 LB Broth (Laurie Bertani) - 500ml $8.39

Quality Biological 351-033-721 Magnesium Chloride 1m - 4 x 100ml $34.74

Quality Biological 114-055-101 Normal Saline - 500ml $7.10

Quality Biological 114-058-101CS PBS (1X) pH 7.4 w/o CaMg - 10 x 500ml $69.88

Quality Biological 114-058-101 PBS (1X) pH 7.4 w/o CaMg - 500ml $6.98

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Quality Biological 119-069-131CS PBS 10X pH 7.4 - 10 x 1L $216.50

Quality Biological 119-069-131 PBS 10X pH 7.4 - 1L $21.72

Quality Biological 120-095-721 Penicillin-Streptomycin 100x - 4 x 100ml $38.38

Quality Biological 112-024-101 RPMI 1640 w/o L-Glutamine - 500ml $7.98

Quality Biological 340-031-671 SOC Medium - 10 x 10ml $33.23

Quality Biological 351-036-101 Sodium Chloride, 5M - 500ml $10.06

Quality Biological 351-001-131 TBE Buffer (10X) - 1L $21.92

Quality Biological 351-086-101CS TBS 10x pH 7.4 Case - 10 x 500ml $155.44

Quality Biological 351-086-101 TBS pH 7.4 (10X) - 500ml $15.45

Quality Biological 351-011-131 TE pH 8.0 - 1L $19.14

Quality Biological 351-006-101 Tris-HCL 1M pH 7.5 - 500ml $13.43

Quality Biological 351-007-101 Tris-HCL 1M pH 8.0 - 500ml $13.43

Quality Biological 118-093-721 Trypsin-EDTA 0.25% - 4 x 100ml $28.90

Quality Biological 118-162-131 Water, Cell Culture Grade (Ultra Pure) -1L $15.90

Quality Biological 351-029-721 Water, Molecualr Biology Grade - 4 x 100ml $29.51

Quality Biological 351-029-131 Water, Molecular Biology Grade - 1L $11.19

Quality Biological 351-161-671 Water, PCR Qualified Pure - 10 x 10ml $86.12

Roche Applied Science Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing

Roche Applied Science 11836153001 cOmplete Mini - 25 tablets $125.94

Roche Applied Science 4693132001 cOmplete EASYpack, EDTA-free - 20 tablets $307.17

Roche Applied Science 11873580001 cOmplete EDTA-free - 20 tablets $274.36

Roche Applied Science 11836170001 cOmplete Mini, EDTA-free - 25 tablets $126.83

Roche Applied Science 11697498001 cOmplete Protease Inhibitor - 20 tablets $269.28

Roche Applied Science 10236276001 DAPI (staining reagent) - 10mg $68.67

Roche Applied Science 10104159001 DNase I (Grade II) - 100mg $57.54

Roche Applied Science 11969064001 dNTP Set PCR-Grade - 4 X 25µmol $186.18

Roche Applied Science 11814320001 Formamide, Liquid - 500ml $59.44

Roche Applied Science 10901393001 Glycogen - 20mg $125.70

Roche Applied Science 4707494001 LightCycler 480 Probes Master $260.13

Roche Applied Science 11383213001 NBT Solution - 3ml $54.29

Roche Applied Science 11666789001 PBS Buffer (10X), premixed - 4L $126.67

Roche Applied Science 4906837001 PhosSTOP - 20 tablets (10ml ea) $226.35

Roche Applied Science 4906845001 PhosSTOP - 10 tablets $115.47

Roche Applied Science 3115879001 Proteinase K - 100mg $90.02

Roche Applied Science 3115828001 Proteinase K Solution, PCR Grade - 5ml $142.91

Roche Applied Science 11635379001 Rapid DNA Ligation Kit - 40rxns $156.60

Roche Applied Science 6366244001 X-tremeGENE HP DNA Transfection Reagent - .4ml $183.74

Sigma-Aldrich Some special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing

Sigma-Aldrich A6964-100ML Accutase - 100ml $29.69

Sigma-Aldrich A6013-500G Agarose, Type I - 500g $277.59

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grcf.jhmi.edu 34

Sigma-Aldrich A9647-100G Albumin Fraction V - 100g $196.10

Sigma-Aldrich A2942-20ML Amphotericin B - 20ml $14.15

Sigma-Aldrich A9518-5G Ampicillin, Sodium Salt - 5g $24.38

Sigma-Aldrich A2220-1ML Anti-Flag M2 AB Agarose - 1ml $384.00

Sigma-Aldrich A5955-100ML Antibiotic Antimycotic (100X) - 100ml $29.74

Sigma-Aldrich A7699-1G ATP (Disodium Salt) - 1g $66.60

Sigma-Aldrich C1389-1G Carbenicillin - 1g $74.67

Sigma-Aldrich D5796-500ML DMEM, high, w/L-Glutamine - 500ml $4.96

Sigma-Aldrich D2650-100ML DMSO - 100ml $135.82

Sigma-Aldrich D2650-5X5ML DMSO - 5 x 5ml $45.61

Sigma-Aldrich DN25-100MG DNase I - 100mg $58.71

Sigma-Aldrich D7295-.5ML dNTP Mix (10mM) 5µmol each - .5ml $45.01

Sigma-Aldrich D9663-10ML Donkey serum - 10ml $27.70

Sigma-Aldrich D9891-1G Doxycycline - 1g $40.90

Sigma-Aldrich D8537-500ML DPBS (1X) w/o CaMg - 500ml $4.96

Sigma-Aldrich D9163-5G DTT - 5g $56.35

Sigma-Aldrich E7889-100ML EDTA Disodium Salt - 100ml $45.89

Sigma-Aldrich E9644-.2MG Epidermal Growth Factor (EGF)Human - 0.2mg $179.01

Sigma-Aldrich E1510-10ML Ethidium Bromide - 10ml $50.43

Sigma-Aldrich F2442-500ML Fetal Bovine Serum , U.S. origin - 500ml $135.85

Sigma-Aldrich F4135-500ML Fetal Bovine Serum, Heat Inactivated - 500ml $164.47

Sigma-Aldrich F6178-500ML Fetal Bovine Serum, Qualified - 500ml $124.91

Sigma-Aldrich F0291-25UG Fibroblast growth factor - 25µg $219.88

Sigma-Aldrich F2006-1MG Fibronectin - 1mg $96.66

Sigma-Aldrich F1141-2MG Fibronectin - 2mg $176.40

Sigma-Aldrich F9037-100ML Formamide - 100ml $32.20

Sigma-Aldrich F7503-1L Formamide - 1L $86.47

Sigma-Aldrich NA0300-1KT GenElute™ HP Plasmid Maxi Kit - 10preps $158.62

Sigma-Aldrich PLN70-1KT GenElute™ Plasmid miniprep Kit - 70preps $98.30

Sigma-Aldrich WGA2-50RXN GenomePlex® Complete WGA Kit- 50rxns $298.49

Sigma-Aldrich G6279-1L Glycerol, SigmaUltra - 1L $79.38

Sigma-Aldrich G9023-10ML Goat Serum - 10ml $27.70

Sigma-Aldrich H9394-500ML HBSS w/o CaMg - 500ml $4.96

Sigma-Aldrich H4034-100G Hepes - 100g $52.04

Sigma-Aldrich H8889-500ML Histopaque®-1077 - 500ml $158.91

Sigma-Aldrich H1009-100ML Hydrogen Peroxide - 100ml $40.60

Sigma-Aldrich I9516-500ML Isopropanol - 500ml $47.14

Sigma-Aldrich I3146-5ML ITS Liquid Supplement - 5ml $25.53

Sigma-Aldrich D8187-250UN JumpStart Red Taq DNA - 250u $44.61

Sigma-Aldrich K0879-50MG Kanamycin - 50mg $19.85

Sigma-Aldrich L2020-1MG Laminin - 1mg $94.19

Sigma-Aldrich L0543-10EA LB Agar Plates Kanamycin-50µg/ml - 10ea $25.22

Sigma-Aldrich L2542-500ML LB Broth - 500ml $18.55

Sigma-Aldrich M7145-100ML MEM NEAA (100x) - 100ml $14.57

Sigma-Aldrich M4287-2MG Mitomycin C - 2mg $74.98

Sigma-Aldrich N6530-5ML N1 Medium Supplement (100X) - 5ml $24.60

Supplier Catalog # Description Core Store Price

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Sigma-Aldrich N3910-24ML Neutralization Solution B - 24ml $50.23

Sigma-Aldrich P6148-500G Paraformaldehyde - 500g $27.49

Sigma-Aldrich P0781-100ML Penicillin-Streptomycin - 100ml $10.19

Sigma-Aldrich P5318-5MG Pepstatin A - 5mg $51.30

Sigma-Aldrich P4557-400ML Phenol- Tris Saturated , Equilibrated - 400ml $117.37

Sigma-Aldrich P3803-100ML Phenol: Chloroform 25:24:1 - 100ml $59.95

Sigma-Aldrich P0044-1ML Phosphatase Inhibitor Cocktail 3 - 1ml $62.90

Sigma-Aldrich P5726-1ML Phosphatase Inhibitor Cocktail II - 1ml $57.50

Sigma-Aldrich P6407-5MG Poly-D-Lysine - 5mg $32.41

Sigma-Aldrich P1024-100MG Poly-D-Lysine-100mg $147.26

Sigma-Aldrich P1524-100MG Poly-L-Lysine - 100mg $129.50

Sigma-Aldrich P2714-1BTL Protease Inhibitor - 1 bottle $41.99

Sigma-Aldrich P8340-1ML Protease Inhibitor Cocktail - 1ml $65.70

Sigma-Aldrich P8340-5ML Protease Inhibitor Cocktail - 5ml $139.76

Sigma-Aldrich P8833-25MG Puromycin - 25mg $66.06

Sigma-Aldrich F7425-.2MG Rabbit-Anti flag - 0.2mg $287.50

Sigma-Aldrich R4775-1.2ML Redextract-N-amp kit - 1.2ml $102.34

Sigma-Aldrich XNAT-100RXN REDExtract-N-Amp™ Tissue PCR Kit - 100rxns $227.00

Sigma-Aldrich R2523-100RXN RedTaq Ready Mix PCR Mix - 100rxns $58.65

Sigma-Aldrich R0278-50ML Ripa Buffer - 50ml $45.14

Sigma-Aldrich R0901-100ML RNAlater - 100ml $101.17

Sigma-Aldrich R2020-250ML RNaseZap - 250ml $45.17

Sigma-Aldrich R8758-500ML RPMI-1640 Medium - 500ml $4.96

Sigma-Aldrich S8636-100ML Sodium Pyruvate - 100ml $6.49

Sigma-Aldrich S5016-500G Sucrose ACS Reagent - 500g $13.95

Sigma-Aldrich S4438-100RXN SYBR Green Jumpstart Taq Readymix- 100rxns $105.51

Sigma-Aldrich T9039-10PAK Tris Buffered Saline, ph 8.0 - 10/pkgs $36.26

Sigma-Aldrich T2194-1L Tris-HCL, 1M, pH 7.4 - 1L $35.88

Sigma-Aldrich T9284-100ML Triton X 100 - 100ml $53.13

Sigma-Aldrich T6066-1KG Trizma® Base - 1kg $84.13

Sigma-Aldrich T1503-5KG Trizma® Base - 5kg $234.53

Sigma-Aldrich T4049-100ML Trypsin-EDTA 0.25% - 100ml $9.75

Sigma-Aldrich P7949-100ML Tween 20 - 100ml $28.89

Thermo Scientific Fermentas Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

Fermentas K0171 2X PCR Master Mix- 100rxns $111.05

Fermentas R0611 6X Loading Dye Solution - 5 x 1ml $33.22

Fermentas R0192 dNTP Mix 10mM - 1ml $95.86

Fermentas K1081 DreamTaq™ Green PCR Master Mix (2X) - 200rxns $89.22

Fermentas R0861 DTT - 5g $74.03

Fermentas FD0054 FastDigest® BamH I - 800rxns $49.35

Fermentas FD0084 FastDigest® Bgl II - 200rxns $49.35

Fermentas FD0274 FastDigest® EcoRl - 800rxns $49.35

Fermentas FD0594 FastDigest® Not 1 - 50rxns $63.59

Supplier Catalog # Description Core Store Price

grcf.jhmi.edu 36

Fermentas FD0684 FastDigest® Xba I - 300rxns $58.84

Fermentas FD0694 FastDigest® Xho I - 400rxns $49.35

Fermentas K0692 GeneJET™ Gel Extraction Kit - 250preps $314.16

Fermentas K0691 GeneJET™ Gel Extraction Kit - 50preps $70.23

Fermentas K0503 GeneJET™ Plasmid Miniprep Kit - 250preps $257.21

Fermentas K0502 GeneJET™ Plasmid Miniprep Kit - 50preps $62.64

Fermentas SM0323 GeneRuler™ 100bp DNA Ladder Plus, RTU- 100apps $73.08

Fermentas SM0243 GeneRuler™ 100bp DNA Ladder, RTU - 100apps $60.74

Fermentas SM0313 GeneRuler™ 1kb DNA Ladder - 500apps $152.81

Fermentas SM1333 GeneRuler™ 1kb DNA Ladder Plus - 500apps $164.20

Fermentas R0561 Glycogen - 0.5ml $55.05

Fermentas R0391 IPTG - 1g $41.76

Fermentas K0222 Maxima™SYBR Green qPCR Master Mix (2X) -10 x 1.25ml $615.97

Fermentas K0221 Maxima™SYBR Green qPCR Master Mix (2X) -2 x 1.25ml $145.21

Fermentas 26619 PageRuler™ Prestained Protein Ladder Plus,RTU-100apps $125.28

Fermentas 26616 PageRuler™ Prestained Protein Ladder, RTU-100apps $104.40

Fermentas 26614 PageRuler™ Unstained Protein Ladder-100apps $83.52

Fermentas EO0491 Proteinase K - 1ml $42.71

Fermentas K1422 Rapid DNA Ligation Kit - 50rxns $118.64

Fermentas EO0381 RiboLock™ RNase Inhibitor - 2,500u (40u/µl) $50.30

Fermentas EN0531 RNase A (DNase & protease free) - 1ml(10mg/ml) $37.96

Fermentas 26634 Spectra™ Multicolor Broad Range Ladder - 50apps $110.10

USB-Affymetrix Special orders are available. Check http://grcf.jhmi.edu/core-store/ for a complete listing.

USB-Affymetrix 78200 1 ML ExoSAP-IT For PCR Product Clean-Up - 1ml $394.40

USB-Affymetrix 78200 200 UL ExoSAP-IT For PCR Product Clean-Up - 200µl $100.57

USB-Affymetrix 19943 1 LT Paraformaldehyde Solution, 4% in PBS - 1L $49.30

USB-Affymetrix 75650 200 RXN VeriQuest Probe qPCR Master Mix (2X) - 200rxns $252.54

USB-Affymetrix 75600 200 RXN VeriQuest SYBR Green qPCR Master Mix (2X) - 200rxns $240.81

USB-Affymetrix 75665 200 RXN VeriQuest SYBR Green qPCR Master Mix with Fluoresc $240.81