-Types of Solutions
-Facilitated Diffusion
-Active Transport
Bellwork1. What is diffusion?2. What is osmosis?3. Draw a picture of the same
concentration of solutes inside a cell as outside a cell.
4. Draw a hyper person.
Have data out and ready to hand in
Dialysis Tubing
Is also IMPERMEABLE to sucrose… Which is good, because I made a bunch of
sucrose solutions of different concentrations, and I can’t remember which is which.
Dialysis Lab – Your Job Objective: To determine which of the
unknown solutions is High, Medium and Low concentration.
Purpose: To observe the process of osmosis first hand and to use this process to understand how dialysis works.
Materials 3 Unknown colored solutions (High, Med
and Low Concentration) Water (150 mL) 3 beakers* 3 pieces of dialysis tubing* 3 pipettes Scissors Scale Paper towel
PROCEDURES: Watch demo, and write down procedures
Lab Title: Osmosis Lab (+ catchy title) Problem/Question: Does ______________ of
_________ in the bag affect the final ________ of the bag in ___ after in soaks in water.
Hypoth: if _________________ (color solution) has
(highest/lowest solute concentration) relative to the
Then _________________ As measured by __________
Data table: Mass of dialysis tubes in grams
COLOR INITIAL FINAL Difference High/Low/Med
Red
Blue
Yellow
Diagram:Draw all 3 set ups at the start of the experiment and 15 minutes later.-Include particulate drawings showing H20, and sucrose molecules.-Label the solute and the solvent. -Use arrows to show the flow of water
Materials:
Procedures:
Data Table:
Qual. Observations
Graph: line/bar Conclusion
Names for concentrations:
Know Your Prefixes!!!
Iso – sameHypo – LowHyper – High
Tonic – Liquid/Solution
Hypertonic Hyper= more/too much Higher concentration solution outside the
cell
Isotonic Iso= same Same concentration outside the cell as
inside
Hypotonic Hypo= less/too little Lower concentration solution outside the cell
Passive TransportOxygen and Carbon dioxide enter and leave the cell through simple diffusion
Passive Transport
Small unpolarized (not ions) molecules can pass through the cell membrane without a protein channel.
Slate Practice Lets revisit our salt water example
According to simple diffusion, draw what will happen here.
Slate Practice The solute will spread out until it is evenly dispersed.
Draw what happens to a cell with lots of solute in it, dropped in a beaker of pure H2O? Remember that the cell membrane is permeable to water but not solutes.
How does this work in cells?
Water follows solute into the cell… and the cell swells up.
How does this work in cells?
If it swells up too much, the cell can burst if the membrane gets pulled apart.
How does this work in cells?
FACILITATED DIFFUSION (another easy way – NO energy
required!)molecules Glucose, sodium ions
and chloride ions need help (facilitated) getting across membrane
Carrier proteins help
Active TransportEnergy (ATP) required for movement.
Solutes are moved AGAINST the concentration gradient. (from low to high conc.)
Notes Check
Look back at your notes for Passive and Active Transport.List two differences between passive transport and active transport.
Name one thing they have in common!
Active vs. Passive Transport Differences
Active Uses ATP- This is the energy source for the cell.
Active goes against the gradient Similar
Both have carrier proteins Both move molecules that cannot go through the
membrane on their own
Endocytosis- cell takes in substances Exocytosis- cell releases substances (out) ACTIVE TRANSPORT – requires ATP (energy)
How do cells move really large molecules in and out of the cell?
A portion of the cell membrane surrounds the desired molecule outside the cell.
The cell membrane rejoins pinching off a sac-like organelle called a vesicle.
There are 2 types of endocytosis: phagocytosis and pinocytosis.
Endocytosis
The reverse of endocytosisWastes and cell products are packaged by the golgi body in sacs called vesicles.
These vesicles fuse with the cell membrane and the materials are secreted out of the cell.
Exocytosis
Cellular Transport
Requires ______
Cellular Transport
Requires ______
Passive Transport: Diffusion:
Osmosis:
Hypertonic solution:
Hypotonic solution:
Isotonic solution:
Facilitated diffusion:
Glucose transport:
Active Transport:
Na+/K+ Pump:
Osmosis
Facilitated Diffusion
Glucose transportHypertonic
HypotonicIsotonic
Oxygen &Carbon Dioxide
Diffusion
Transport
PASSIVE
EndocytosisExocytosis
ACTIVE
Proteins and ATP
Na+/K+ Pump
Moves with
gradient
Moves against
gradient
ATP
Your Crazy Study Page For Mon’
Test
Don’t forget: Draw a
phospholipid bilayer with proteins and
carbohydrates (& their
functions)
Solution:Solute-particleSolvent-liquid
Diffusion OsmosisPassive vs. Active
ENDO EXO
1. What is the difference between active and passive transport?
2. LIST 2 kinds of passive transport.3. With a concentration gradient,
molecules move from areas of ____ concentration to areas of ____ conc.
4. During osmosis, if there is more salt inside the cell than outside, which way would water move?
Closure