BIO111 5
Yeast
?
( )
1. (DNA) ()
2. 4 -
3. I.II.III.5->3IV.->CV. VI.3 =1
-To
You are here
http://www.biologie.uni-hamburg.de/lehre/bza/1ihf/e1ihfm.htm
DNA
IHF(E. coli)
HU(B. stearothermophilus)
TF1(B. subtilis)
http://www.biologie.uni-hamburg.de/lehre/bza/1ihf/e1ihfm.htm
DNA IHF(Integration Host Factor)
E. coli
DNA
DNA
1. 2.
3. ()
-/
...
...DNA
DNA
DNA
DNA
-
-
1 1
1 RNA
=
-
DNA?
-, .
RNA.
RNA
1. 2. O
3. ()
M = 5' -> 3'
A K
1.DNA ()2.RNA (+)3.4.ATP5. 6.
?
5 3
T85T83G81A61C68A52
T89A81T50A65A65T100
-
?
- RNA
RNA
- +
-
RNA ?
5 3
70 DNA?
E.coli
CD-ROM
+1 G
-
DNA
http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/chroms-genes-prots/RNAP-supercoil.html
RNA
-
G
U
CCC
UC
AU
GGG
=
=
= U U U
U
G
UCA
UA
CU
AGU
=
=
=
GC
AU
M
-
RNA?
-, .
M = H2 COOH mRNA
5' -> 3'
(
)
()
, , C, S, P, H ....
DNA, RNA
A K
1.mRNA2.tRNA-aa3.30S+50S = 4.GTP, ATP5. 6. 7.
-
-35 -10 +1
5 3
5
5
3
3
RNA
5 UTR 3 UTR
-
-35 -10 +1
5 3
5
5
3
3
RNA
5 UTR 3 UTR
C
-
-35 -10 +1
5 3
5
5
3
3
RNA
Shine-Dalgarno
AUUCCUCCAC
30S 16S RNAUAGXXX
OH
XXXAGG GG GA AU U CXXXXXXAUGXXX...
5'-
-3'mRNA
Ribosome inding Site A
Shine-Dalgarno : AGGAGGU
SHINE-DALGARNO
3'
tRNA
tRNA
tRNA mRNA
E
-
-
CD-ROM
-
DNA
CD-ROM
..
()
?
H -GroEL-ES
Top view into the cavity of the top ring. The domain rotation caused by nucleotide binding continues into the large movement accompanying GroES binding.
Bottom view.Different pattern of movement in the lower ring. The upper ring (at the back of the images) performs a continuous twisting motion, but the lower ring opens and shuts with radial movements of the domains in addition to the twisting.
-
?
=
mRNA
SD
mRNASD SD
SD
mRNASD SD
SD
1 2 3
SD
SD
SD
?
:Mycoplasma genitalium 482
580.070 bp 482
RNA 12 470
. 5.3%. 8.0%. 45.0%V. 13.0%V. 16.6%VI. 8.2%
ONII ( 1999)
X
.
(Mbases)
o
Saccharomyces cerevisiae 12.069 6.294 3.089 63%Escherichia coli - 4.639 4.289 2.656 62%
Mycobacterium tuberculosis (strain -H37Rv) + 4.447 4.402
Bacillus subtilus + 4.214 4.099 ~2.320 ~58%
Synechocystis sp. - 3.573 3.168 1.402 48%
Archaeoglobus fulgidus 2.178 2.471 1.193 44%Haemophilus influenzae - 1.830 1.850 1.015 58%
Methanobacterium thermoautotrophicum 1.751 1.855 816 44%Pyrococcus horikoshii (shinkaj) 1.738Helicobacter pylori - 1.667 1.590 907 57%
Methanococcus jannaschii 1.664 1.692 776 46%Aquifex aeolicus (strain VF5) - 1.551 1.554
Borrelia burgdorferi - 1.230 1.256 499 58%
Treponema pallidum - 1.138 1.041
Rickettsia prowazeckii B- 1.112 840 526 62%Chlamydia trachomatis - 1.042 896
Mycoplasma pneumoniae + 0.816 677 333 49%
Mycoplasma genitalium + 0.580 488 324 69%
= , = (Gram+ -) =. . ( ) http://www.ncbi.nlm.nih.gov/PMGifs/Genomes/micr.html