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Page 1: buffer

10 5 10 5bu

ffer

β-actin

(ng/ml)

wt ΔC12

WT ∆C12

2025

kDa

CBB stained

10

15

A B

Supplementary Figure S14

Supplementary Figure S14. L1-RTP by Vpr required a carboxy-terminal region.A. 　 Purification of rVpr and ΔC12. Each rVpr were purified using two-step column chromatography at the same time. Purified proteins were stained with Coomassie brilliant blue (CBB). B. Dependence of rVpr induced L1-RTP on its C-terminal region. HuH-7 cells were transfected with pEF06R and selected with Puro. Replated cells were treated with indicated amouts of either Wt or ΔC12 of rVpr for 2days. rVpr induced L1-RTP was analyzed by PCR based assay. Apparent L1-RTP induction was not observed by C-terminus truncated rVpr treatment.

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