CAVIMC 040 mAb Linear Epitope Mapping
Shaunna Shen/Georgia Tomaras, CAVIMC
• 24 mAb + 2 negative control tested: 20 µg/ml (default), 5, 10, or 40 µg/ml (for repeats/confirmation).
• Peptide sequences on slide: 15-mers overlapping by 12, covering full length consensus Env gp160 of HIV clade A, B, C, D, group M, and CRF 1 & 2.
• Slide Lots used: JPT 1948 (default) and JPT 2049 (for repeats/confirmation).
• X-axis: Peptide#, ordered from N’ to C’ on gp160.
• Y-axis: Signal intensity, as determined by image analysis of the chip scans.
• Colors of bars represent different clades/group/CRFs of HIV Env.
• Background subtraction: 5-fold the signal intensity for nonspecific peptide binding.
• Results are summarized in slide 3 as a table. Graphs of data for each mAb follow (Note: In the graphs, the y-axis scales differ based on response magnitude). Binding intensity values per clade for each mAb with positivity calls are shown in a table after the graph.
CAVIMC 040 mAb Linear Epitope Mapping
# mAb Name source Specificity Linear Epitope MappedN12-i9.1 Human IgG1 Neg Ctrl NegativeN12-i9.1 Human IgG1 Neg Ctrl Negative2F5 Human IgG1 2F5 epitope 2F5 epitope
1 N5-i5 Human IgG1 gp120 Negative2 N12-i3 Human IgG1 gp120 Negative3 N26-i1 Human IgG1 gp120 Negative4 L9-i1 Human IgG1 gp120 gp415 L9-i2 Human IgG1 gp120 Negative6 JR48.1 Human IgG1 gp120 C17 JR49 Human IgG1 gp120 C58 A32 Human IgG1 gp120 Negative9 C11 Human IgG1 gp120 Negative
10 N60-i3 Human IgG1 gp120 Negative11 JR4 Rhesus IgG1 gp120 Negative12 JR20 Rhesus IgG1 gp120 Negative13 JR52 Rhesus IgG1 gp120 C514 N12-i15 Human IgG1 gp120 C415 N60-B1.1 Human IgG1 gp120 Negative16 N5-U1 Human IgG1 gp41 Negative17 N5-U2 Human IgG1 gp41 Negative18 N5-U3 Human IgG1 gp41 Negative19 N10-U1 Human IgG1 gp41 gp41 ID, Clade C C1 & MPER20 N10-U2 Human IgG1 gp41 gp41 CBD-121 M785-U1 Rhesus IgG1 gp41 gp41 ID22 M785-U2 Rhesus IgG1 gp41 gp41 CBD-123 M785-U3 Rhesus IgG1 gp41 Negative24 M785-U4 Rhesus IgG1 gp41 gp41 CBD-1
SUMMARY OF
RESULTS
N12-i9.1 Neg Ctrl
N12-i9.1 (Neg Ctrl) 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N12-i9.1 (Neg Ctrl) 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-O1 Neg ctrl
N5-O1 (Neg Ctrl) 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-O1 (Neg Ctrl) 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
2F5 Pos Ctrl
2F5 10g/ml_gp41 IgG_PMT540_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
2F5 10g/ml_gp120 IgG_PMT540_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-i5
N5-i5 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N5-i5 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N12-i3
N12-i3 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N12-i3 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N26-i1N26-i1 10g/ml_gp120 IgG_PMT600_24Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N26-i1 10g/ml_gp41 IgG_PMT600_24Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPERImmunodominant
HR2
Kennedy Sequence LLP2
L9-i1L9-i1 10g/ml_gp120 IgG_PMT600_24Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
na
l In
ten
sit
y
0
2000
4000
6000
8000
10000
12000
14000Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
L9-i1 10g/ml_gp41 IgG_PMT600_24Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
na
l In
ten
sity
0
2000
4000
6000
8000
10000
12000
14000 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPERImmunodominant
HR2
Kennedy Sequence LLP2
L9-i1 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
200 295 0 0 0 0 0 0
201 0 1690 276 194 0 0 2138
202 0 0 148 0 0 0 0
203 0 0 0 0 62 0 0
214 1005 0 519 0 0 0 0
10 µg/ml, Lot # JPT2049, PMT600; SXS 23-11
Background subtraction: 5-fold nonspecific peptide binding.
L9-i2
L9-i2 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
L9-i2 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR48.1JR48.1 20g/ml_gp120 IgG_PMT540_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
1.0e+5
2.0e+5
3.0e+5
4.0e+5
5.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5C5
C1
JR48.1 20g/ml_gp41 IgG_PMT540_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
500
1000
1500
2000
2500
3000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
JR48.1 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
9 68 528 130 346 68 0 57
10 15016 6631 1432 4698 15016 12693 6680
11 23081 17655 7062 17655 23081 20499 4038
12 63124 46498 44927 46498 46498 49918 59875
13 566 689 469 689 689 787 0
10 µg/ml, Lot # JPT2049, PMT600; SXS 23-11
Background subtraction: 5-fold nonspecific peptide binding.
JR49JR49 20g/ml_gp120 IgG_PMT540_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR49 20g/ml_gp41 IgG_PMT540_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
JR49 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
157 126 55068 0 22140 55068 259 0
158 163 44526 0 39524 44526 517 0
20 µg/ml, Lot # JPT1948, PMT540; SXS 23-06
Background subtraction: 5-fold nonspecific peptide binding.
A32
A32 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
A32 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
C11
C11 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
C11 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N60-i3
N60-i3 20g/ml_gp41 IgG_PMT600_07Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
na
l In
ten
sity
0
2000
4000
6000
8000
10000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N60-i3 20g/ml_gp120 IgG_PMT600_07Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
500
1000
1500
2000
2500
3000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR4
JR4 (mk) 20g/ml_gp41 IgG_PMT600_27Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
JR4 (mk) 20g/ml_gp120 IgG_PMT600_27Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR20JR20 (mk) 20g/ml_gp120 IgG_PMT600_27Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR20 (mk) 20g/ml_gp41 IgG_PMT600_27Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
JR52JR52(mk) 20g/ml_gp120 IgG_PMT580_27Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
JR52(mk) 20g/ml_gp41 IgG_PMT580_27Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
JR52 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
159 0 2688 0 0 0 0 0
160 13302 44634 13770 10031 12870 13357 11602
161 673 26587 482 288 482 673 115
162 0 12116 0 0 0 0 0
20 µg/ml, Lot # JPT1948, PMT580; SXS 23-09
Background subtraction: 5-fold nonspecific peptide binding.
N12-i15N12-i15 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
na
l In
ten
sit
y
0
2000
4000
6000
8000
10000
12000
14000 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N12-i15 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
na
l In
ten
sity
0
2000
4000
6000
8000
10000
12000
14000 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N12-i15 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
138 0 0 0 7099 0 0 0
20 µg/ml, Lot # JPT1948, PMT600; SXS 23-10
Background subtraction: 5-fold nonspecific peptide binding.
N60-B1.1
N60-B1.1 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N60-B1.1 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-U1N5-U1 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-U1 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N5-U2N5-U2 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-U2 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N5-U3N5-U3 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N5-U3 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
1000
2000
3000
4000
5000
6000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
N10-U1
N10-U1 5g/ml_gp41 IgG_PMT540_24Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
1.0e+5
2.0e+5
3.0e+5
4.0e+5
5.0e+5 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPERImmunodominant
HR2
Kennedy Sequence LLP2
N10-U1 5g/ml_gp120 IgG_PMT540_24Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
1.0e+5
2.0e+5
3.0e+5
4.0e+5
5.0e+5 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N10-U1 Signal Intensity ValuesPeptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
33 0 0 53415 0 0 0 0
189 58960 58960 58978 58960 58960 59156 59086190 59070 59070 59070 59070 59070 53422 59159194 0 0 0 13065 0 0 0195 5306 774 0 26403 5306 0 12130196 0 54161 47183 51288 0 44181 35031199 0 0 0 43418 0 0 0
214 0 0 40230 0 0 0 0
5 µg/ml, Lot # JPT2049, PMT540; SXS 23-11
Note: “Breakage” of the gp41 ID epitope at peptide #192 and 193 is a known slide quality issue. The epitope is very likely a continuous one.
Background subtraction: 5-fold nonspecific peptide binding.
N10-U2N10-U2 20 g/ml_gp120 IgG_PMT600_16Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
2.0e+4
4.0e+4
6.0e+4
8.0e+4
1.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
N10-U2 20 g/ml_gp41 IgG_PMT600_16Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
2.0e+4
4.0e+4
6.0e+4
8.0e+4
1.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
CBD-1
N10-U2 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
198 0 0 0 0 0 0 0
199 0 843 0 8488 0 0 8634
200 0 21108 0 0 3140 0 2347
201 0 13024 13695 21420 9405 0 3795
202 0 0 5522 6134 7654 0 0
203 0 0 0 0 0 0 0
20 µg/ml, Lot# JPT1948, PMT600; SXS 23-10
Background subtraction: 5-fold nonspecific peptide binding.
M785-U1
M785-U1(mk) 20g/ml_gp41 IgG_PMT580_27Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
3.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
M785-U1(mk) 20g/ml_gp120 IgG_PMT580_27Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
3.0e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
M785-U1 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
192 38178 38178 38178 0 38178 9730 38178193 16455 13054 13054 4931 16455 538 12801
194 22182 20079 21843 4928 22182 868 18114
20 µg/ml, Lot# JPT1948, PMT580; SXS 23-09
Background subtraction: 5-fold nonspecific peptide binding.
M785-U2
M785-U2(mk) 40g/ml_gp41 IgG_PMT600_24Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
na
l In
ten
sity
0
2000
4000
6000
8000
10000
12000
14000 Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPERImmunodominant
HR2
Kennedy Sequence LLP2CBD-1
M785-U2(mk) 40g/ml_gp120 IgG_PMT600_24Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
na
l In
ten
sit
y
0
2000
4000
6000
8000
10000
12000
14000Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
M785-U2 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
201 0 1285 0 2233 2403 0 0
40 µg/ml, Lot# JPT2049, PMT600; SXS 23-11
Background subtraction: 5-fold nonspecific peptide binding.
M785-U3M785-U3 (mk) 20g/ml_gp120 IgG_PMT600_27Mar2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sit
y
0
500
1000
1500
2000
2500
3000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
M785-U3 (mk) 20g/ml_gp41 IgG_PMT600_27Mar2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0
500
1000
1500
2000
2500
3000
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPER
Immunodominant
HR2
Kennedy Sequence
LLP2
M785-U4M785-U4(mk) 40g/ml_gp120 IgG_PMT580_24Apr2012
gp120 peptide number0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
V3
V2V1
V4 V5
C5
C1
M785-U4(mk) 40g/ml_gp41 IgG_PMT580_24Apr2012
gp41 peptide number
165 170 175 180 185 190 195 200 205 210 215 220 225 230 235 240 245 250 255 260 265 270 275
Sig
nal
In
ten
sity
0.0
5.0e+4
1.0e+5
1.5e+5
2.0e+5
2.5e+5
Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2 MPERImmunodominant
HR2
Kennedy Sequence LLP2
CBD-1
M785-U4 Signal Intensity Values
Peptide # Clade A Clade B Clade C Clade D Group M CRF 1 CRF 2
199 0 5598 0 0 1016 0 3438
200 2014 13458 2344 0 14384 2819 16795
201 1174 33507 27116 35009 33027 0 35006202 2552 7963 20051 23515 21665 0 2552
203 0 8507 0 8507 18555 0 0
40 µg/ml, Lot# JPT2049, PMT600; SXS 23-11
Background subtraction: 5-fold nonspecific peptide binding.