CECT 516 Escherichia coli(Migula 1895) Castellani and Chalmers 1919
Other culture collections: ATCC 8739; CCRC 11634; CCTM La 2194; CCUG 10979; CIP 53.126; DSM 1576; Gunsalus strain Crookes; IFO 3972; IMET 11121; LMG 8063; NCFB 904; NCIMB 8545; PCM 2561; VTT E-76039; WDCM 00012
History: CECT, 1982 < NCIMB, 1953 < ATCC < I. C. Gunsalus < G. C. Crooks Isolation source: Human faeces
Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214
Risk group: 2Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h
CHARACTERIZATION
Miniaturized biochemical test API 20E
Growth on selective/differential culture media
CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results.
ENDO AGAR
Red colonies, red medium, golden sheen
TERGITOL AGAR
Yellow colonies with yellow zone. Sometimes with rust coloured centre
+ - + - - - - - + * - + + - + + - + * + -
1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4
ONPG ADH LDC ODC CIT H2S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX
5 0 4 4/5 5 5 2/3
Identification: 94.7-99.9% ID Escherichia coli
C E R T I F I E D
C E R T I F I C A D A
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TBX (Tryptone Bile X-glucuronide) AGARBlue-green colonies
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*This result may vary
CECT 516 Escherichia coli(Migula 1895) Castellani and Chalmers 1919
Growth on selective/differential culture media
C E R T I F I E D
C E R T I F I C A D A
CTSM (Sorbitol MacConkeyagar with Cefixime Tellurite) AGARPink colonies
VRBL (Violet Red Bile Lactose Agar) AGAR
Red-purple colonies with or without halos
VRBGA (Violet Red Bile Glucose Agar) AGAR
Red-purple colonies surrounded by purple haloes
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MYP (Mannitol Egg YolkPolymyxin) AGARGrowth inhibition
Left: Brilliant Green Bile Lactose BrothRight: Laurylsulfate Tryptose BrothBoth: Turbidity with gas production
EC Broth
Turbidity with gas production