Crown rust resistance of oat germplasm from the ARS-
Aberdeen program J. M. Bonman (1), D.E. Obert (1), E. W. Jackson (1), S.A. Harrison (2) and M. L. Carson (3). (1)USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1691 S. 2700 W., Aberdeen ID 83210; (2) Louisiana State University, AgCenter Agronomy Department, 104 MB Sturgis Hall, Baton Rouge, LA 70803; (3) USDA-ARS Cereal Disease Laboratory1551 Lindig Ave., St. Paul, MN 55108.
Where is Aberdeen?
University of Idaho Research and
Extension Center, Aberdeen, ID
Background
• Previously the program targeted the Intermountain West– objective was high yield and quality
• Currently a need to make Aberdeen material more widely useful by incorporating disease resistance
Objectives
Characterize crown rust resistance within Aberdeen cultivars and breeding lines: Field reactions in “hot spot” locations (Castroville,
TX; Baton Rouge, LA) Seedling resistance to many isolates (CDL) Field reactions to single isolates (isolated plots in
Aberdeen, ID) Assess partial resistance of selected materials in
greenhouse inoculations with compatible isolates
Test lines and cultivarsLine/Cultivar Pedigree
89Ab3840 75Ab861 / L75-340294Ab6965 87Ab5597 / 86Ab525995Ab11439 PA8393-1500 / 82Ab24895Ab12847 74Ab2608 / Ajay98Ab7085 86Ab1616 / 89Ab286198Ab7399 Park / 88Ab307399Ab11468 NPB871312-3 / Monida99Ab11685 91Ab149 / Prairie99Ab11787 91Ab2 / SP9-799Ab11813 Whitestone / 91Ab299Ab11862 92Ab390 / Whitestone99Ab11899 90Ab1322 / SP9-299Ab12179 90Ab1336 / Preakness99Ab12402 89Ab2861-1 / 88Ab3073 M399Ab12687 3073 / D-5 #4 53-5Ajay 74Ab1952 / 74Ab2608 Lamont 79Ab3811 / S 7884Maverick 80Ab988 / MonidaMonico 83Ab3119 / MonidaMonida Cayuse / OtanaOtana CI5345 / ZansterProvena Pennlo / PI 447276
Check cultivars CDC Boyer IA98822-2
0
25
50
75
100
Sorted entries
Dis
ease
d le
af a
rea
(% o
f ch
eck)
Brooks
CDC BoyerIA98822-2
0
25
50
75
100
Sorted entries
Dis
ease
d le
af a
rea
(% o
f ch
eck)
Brooks
CDC BoyerIA98822-2
ID field plots for single isolate tests
Single isolate testsExperimental units = single hills
Field tests—naturally occurring inoculum
Entry Field Test Location
Louisiana Texas
DLA (%) Classa DLA (%) Class
CDC Boyer (R check) 13.3 check 16.7 check
99Ab11862 18.3 R 20.0 R
IA 98822-2 35.0 R 11.7 R
99Ab12179 36.7 R 16.7 R
99Ab11813 56.7 S 26.7 R
95Ab11439 50.0 S 43.3 R
Monida 31.7 R 65.0 S
Monico 32.5 R 66.7 S
99Ab11468 53.3 S 55.0 S
89Ab3840 66.7 S 50.0 I
94Ab6965 66.7 S 56.7 S
98Ab7085 61.7 S 63.3 S
99Ab11685 56.7 S 73.3 S
99Ab11899 63.3 S 66.7 S
99Ab11787 65.0 S 66.7 S
Maverick 65.0 S 68.3 S
Ajay 80.0 S 55.0 S
Otana 80.0 S 56.7 S
99Ab12402 68.3 S 70.0 S
Lamont 80.0 S 63.3 S
99Ab12687 76.7 S 70.0 S
Provena (S check) 75.0 check 73.3 check
98Ab7399 80.0 S 70.0 S
95Ab12847 76.7 S 76.7 S
aR = not significantly different from the resistant check CDC Boyer by Dunnett's test (p = 0.05)
S = not significantly different from the susceptible check Provena by Dunnett's test (p = 0.05)
I = intermediate
Field tests—single isolate evaluations
R = not significantly different from the resistant check CDC Boyer by Dunnett's test (p = 0.05)
S = not significantly different from the susceptible check Provena by Dunnett's test (p = 0.05)
I = intermediate
Entry Specific Isolates
98MNB245 03MN111 CR185
DLA (%) Classa DLA (%) Class DLA (%) Class
CDC Boyer (R check) 2.5 check 5.4 check 1.0 check
99Ab11862 0.9 R 12.6 R 0.3 R
IA 98822-2 4.3 R 4.1 R 3.0 R
99Ab12179 10.5 R 13.6 R 2.3 R
99Ab11813 0.4 R 13 R 1.4 R
95Ab11439 8.8 R 17 R 42.0 I
Monida 22.0 R 22 I 5.3 R
Monico 35.0 S 29 S 97.5 S
99Ab11468 23.8 R 29 S 15.0 R
89Ab3840 27.5 S 15.6 R 27.5 R
94Ab6965 52.5 S 26.2 S 87.5 S
98Ab7085 37.5 S 33 S 97.5 S
99Ab11685 8.3 R 12.6 R 5.3 R
99Ab11899 31.3 S 26 S 95.0 S
99Ab11787 23.8 R 40 S 100.0 S
Maverick 40.0 S 31 S 72.5 S
Ajay 23.8 R 27 S 32.5 R
Otana 25.0 S 37 S 100.0 S
99Ab12402 43.3 S 27 S 100.0 S
Lamont 30.0 S 33 S 97.5 S
99Ab12687 22.5 R 29 S 90.0 S
Provena (S check) 47.5 check 38 check 100.0 check
98Ab7399 47.5 S 44 S 100.0 S
95Ab12847 15.8 R 23 S 72.5 S
Greenhouse tests versus a spectrum of isolates
EntryResistance spectrum (%)a
CDC Boyer (R check) 100
99Ab11862 49
IA 98822-2 62
99Ab12179 60
99Ab11813 57
95Ab11439 100
Monida 100
Monico 100
99Ab11468 100
89Ab3840 80
94Ab6965 100
98Ab7085 96
99Ab11685 70
99Ab11899 98
99Ab11787 100
Maverick 100
Ajay 100
Otana 100
99Ab12402 100
Lamont 98
99Ab12687 94
Provena (S check) 100
98Ab7399 100
95Ab12847 100
aPercent compatible P. coronata isolates tested at the Cereal Disease Laboratory, n = 48
Provena
99Ab11862
95Ab11439
99Ab11813
99Ab12179
Leaf segment inoculation technique:
Greenhouse test for partial resistance
aNumbers within a column followed by the same letter are not significantly different by Tukey’s HSD test. Four isolates tested, no significant isolate by genotype interaction detected.
Genotype DLA (%)a FDNA (pg/mg oat tissue)
Provena 17.1 a 1208 a
99Ab11862 13.6 ab 623 b
99Ab12179 10.6 bc 696 b
95Ab11439 9.2 c 638 b
Conclusions
• Most of the Aberdeen cultivars and lines are highly susceptible to crown rust
• A few lines showed less disease in field trials– specific resistance to some isolates– partial resistance to compatible isolates in the
greenhouse, thus field response probably due to partial resistance