CultivationCultivation
To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
Cell cultureCell culture
1. Removal.2. Homogenization.3. Washing.4. Trypsinization.5. Washing.6. Growing.7. Inoculation.8. Incubation.
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
IntracerebrallyIntracerebrally
IntraperitoneallyIntraperitoneally
IntramuscularlyIntramuscularly
IntravenouslyIntravenously
IntranasallyIntranasally
Primary cell culturePrimary cell culture
A culture derived directly from a tissueA culture derived directly from a tissue
Diploid cell cultureDiploid cell culture
Subsequent cultures derived from primary cell culture.Subsequent cultures derived from primary cell culture.
Suspension cell cultureSuspension cell culture
A culture in it the dispersed cells are not allowed to settle down, but A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.they are kept floating by continuous shaking of the flasks.
Permanent cell linePermanent cell line
A culture derived from single separated cell i.e. diploid culture it can be A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely. propagated indefinitely.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of bacterial Cultivation of bacterial virusesviruses
1.1. On broth mediaOn broth media 2. On solid medium2. On solid medium