1RaLabo

Insignifi“precoIn thisgold c

Misoflurscannewere mmaps 40s). TFA = 3, 4 hosourcemetho90 mithe blinput f

ReLnCaptumorhours POST

permeeffectsFigureaccumeffectsmonitRefereal., MaFundedCytImm

DeteIsabelle I

adiology, Universoratory, Minneapo

ntroduction. Reicantly improveonditioning”, has work, we propconstruct (CYT-6

Materials & Merane in O2/N2O ed using an RF vmonitored throuprior to contrastThe same basic 30º, total acquisours after an intre DICOM viewiods using the prenutes of the expood to ve) and kfunction (AIF) wesults. In one cap tumors after inr region is showafter injection o

T, while kep dro

eability and retes were observede 1c. Along with

mulation of theras of CYT in vivor novel precond

ences. 1. Goel, R.,agn Reson Med, 20d by NIH P41 Rmune Sciences, In

ction of the efltis1, Jeunghwan City of Minnesota -

olis, MN, United StStates, 4

ecently, it has bed by using TNFs been studied uose to use Dyna6091) [1] in micethods. Four to(50/50). A cathevolume coil (Mi

ughout the expert injection (FOVacquisition was

sition time = 1h ravenous injectiong software (Ose-contrast T1 maperiment. The pakep (rate constanwas determined fase, the data wentraperitoneal inj

wn (B, average oof CYT. The parops considerably

ention (EPR) [6]d in the muscle, h improving cryapeutic agents pvo with non-desditioning of ther, et al., Mol Cance005. 5. Ocak, I., e

RR008079, The Wnc. for providing C

ffect of nanopaChoi2, Emily Colon

CMRR, Minneapotates, 3Biomedical 4Department of Ur

een shown that thF-� based nanopausing invasive oramic Contrast Ence bearing LnCapo 5 weeks after eter was placed iillipede, Agilentriment. A multisV = 35 mm, matrs used to acquire29min. Animalson of CYT (POSsiriX) [2]. Paramaps [3, 4]. The aarameters ve (exnt between the vfrom a posterior re discarded duejection of Gd (A

of 10 tumors). Inrametric maps oy after CYT, sug

]. The results ofthe greatest chan

yosurgical procedpreferentially witructive method

rapeutic intervener Ther, 2007. 2. Rt al., AJR Am J R

WM KECK FoundCYT-6091.

article preconnna1, Manda Vollmolis, MN, United SEngineering, Uni

rologic Surgery, U

he outcome of crarticles before thr post-mortem mnhanced Imagingp tumors, beforeimplantation onin the intraperitot, Palo Alto) at alice gradient echrix size = 128 xe DCE-MRI dats were scanned oST). Enhanceme

metric maps werearea under the gaxtravascular and ve and plasma) vessel within th

e to movements A). Enhancementn both the tumoof a typical mousggesting slightly

f this study demnges in contrast dures, the retentiithin a tumor, th

ds is important antions will be advRosset, A., et al., J

Roentgenol, 2007. dation and AHC

nditioning in amers1, Mithun SheStates, 2Mechanicaversity of Minneso

University of Minne

ryosurgery, a prohe procedure is

methods in animag (DCE-MRI) ase and after injectn both hindlimboneal cavity for a 9.4T magnet eqho sequence was

x 128, 1 mm thica to follow conton day 1 withou

ent curves were oe obtained by firadolinium conceextracellular vowere calculated

he imaging volumduring the acqut curves of the sir and the musclse are also showy slower “washi

monstrate that enretention are fo

ion of contrast inhus increasing eas such compounvantageous in fuJ Digit Imaging, 2189(4): p. 849. 6.

Translational Re

a mouse modelenoi3, Joel Slaton4,al Engineering, Unota - Bioheat and Mesota, Minneapolis

ocedure gainingperformed [1].

als, but has neves a method to mtion of CYT-609bs of LnCap tumGadolinium-DTquipped with a Vs acquired at muckness, TE = 1.4trast kinetics wiut injection of Cobtained in regiorst converting thentration curve (olume per unit vo

by applying theme to mitigate su

uisition. Figure 1ignal relative to le (not shown),

wn (C). In the tuin” and a substa

tumor, respecslightly increa

between PREincreased (5-fothe AUGC m250% in the tuAUGC maps processing damodeling is dsmall size of th

Discussionthe effect of construct can using DCE-MPreconditioninphysiological

nhanced EPR is ound in the tumon these studies mefficacy while rends progress towuture translationa004. 3. Daldrup, HShenoi, M.M., et esearch Grant Pro

Figure 1.injection (signal enhdynamic cvivo in tuData are mThe solid w

l of prostate c, John Bischof2, anniversity of MinnesMass Transfer Labs, MN, United Stat

g acceptance for The effect of th

er been charactermonitor in vivo th91 (CYT). mors, 6 male nuTPA (Gd) injectiVarian console. ultiple flip angle43 ms, TR = 65 th the followingYT (PRE), recoon of interests (t

he signal changes(AUGC) was deolume of tissue)e two-compartmubject and exper

1 shows an axialthe maximum swe observed th

umor region, Ktrantially slower “ctively. In the ase post-CYT. V

E and POST coold) in the area o

maps show that umor, and by onare independen

ata. This is signdifficult to identhe vessels in then and Conclus

“preconditioninbe detected non

MRI in a moung has been event involving,likely the resul

or as demonstratmay indicate theeducing toxicitywards clinical tral studies. H.E., et al., Magn al., Nanomedicineogram 2009 09-0

Axial view of a m(A). Regions of inhancement at eachcurves of relativeumors before and mean ± SEM. In Cwhite line delineat

ancer by MRInd Gregory J Metzsota - Bioheat and boratory, Minneaptes

prostate cancer this nanoparticlerized in vivo in the effect of a we

ude mice were aon (0.6 mmol/kgTemperature an

es (from 5 to 40ms, 4 averages,

g parameter chanovered, and scanntumor and muscls to concentratio

etermined by int), KTrans (transf

ment Kety modelrimental variatio view of a typic

signal observed aat contrast uptak

rans is slightly lo“washout” of thmuscle, these sVe is comparab

onditions, while of the tumor POGd concentrati

nly 18% in the mnt of the kineticnificant as the Atify reliably in

e imaging volumsion. In this wong” of the tumn-invasively in tuse model of

shown to b, among other chlt of CYT admited in the pharme potential of CYy. The ability torials. Non-invasi

Reson Med, 1998e (Lond), 2011. Ac

08. We also grate

mouse after CYT nterest for the tumoh time point is obe signal enhancem

after CYT treatmC), the parametric tes the tumor regio

I zger1 d Mass Transfer polis, MN, United

treatment, can bon the tumor, o

the intact animaell-defined TNF-

anesthetized witg). Animals wer

nd respiration rat0º) to calculate T, acquisition timnges: 8 averagesned again on dale) using an opeon using standaregrating over thfer constant froml [5]. The arteriaons. al mouse bearinafter CYT for thke is enhanced ower PRE versuhe contrast in thsame parameter

ble in the muscl

it is drasticallOST. Remarkably

ion increases bmuscle. Note thac model used foAIF used for thmice due to th

me rk, we show tha

mor by a TNF-the intact animaprostate cancerbe a complehanges, enhanceinistration. Whil

macokinetic mapsYT to enhance tho characterize thive techniques t

8. 4. Deoni, S.C., ecknowledgements

efully acknowledg

and gado-linium or are drawn and btained (B). The ment obtained in ment are shown. maps are shown.

on.

e or l. -�

h re te T1

me s, y n d e

m al

g he 4

us he rs e

y y, y at or e

he

at -� al r. x d e s, e

he o

et s.

ge

3020Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)