Duke University, Fitzpatrick Institute for Photonics BIOS Lab,
Department of Biomedical Engineering Advances in time-resolved and
spectroscopic quantitative phase microscopy Duke University
Department of Biomedical Engineering Matthew Rinehart Preliminary
Examination 7/24/2012
Slide 2
Outline Summary of course work Peer-reviewed publications
Motivation & introduction Preliminary results QPM technology
QPM applications QPS Research aims & timeline
Slide 3
Summary of course work TermCourseCourse TitleInstructorUnits
Fall 2008PATH 225 Intro to Systemic HistologyL. Hale2 BME
265Advanced OpticsJ. Kim3 BME 233Modern Diagnostic Imaging Systems
J. MacFall3 Spring 2009 ECE 299Imaging & SpectroscopyD. Brady3
PATH 250 General PathologyL. Hale4 Fall 2009ECE 299Holography &
Coherent ImagingD. Brady3 PHY 230Math Methods in PhysicsA. Kotwal3
Spring 2010 BME 265Image ProcessingS. Farsiu3 BIO 154Fundamentals
of NeuroscienceS. Bilbo3 Fall 2010ECE 376Lens DesignD. Brady3
Spring 2011 ECE 399Independent Study: Broadband Diffraction Phase
Microtomography D. Brady3 Fall 2011BME 234Modern MicroscopyA. Wax3
BME 362Invention to ApplicationB. Myers3 Spring 2012 BME
362Invention to ApplicationB. Myers3 RequirementsUnits Advanced
Math3 Life Sciences8 ECE Masters Courses 12 Total:42 RCR Hours8 (of
12) TermCourseCourse TitleInstructor Fall 2009BME 171Intro to
Signals & SystemsJ. Izatt Fall 2010BME 171Intro to Signals
& SystemsJ. Izatt Teaching Experience
Slide 4
System filters & fluorophores Bandpass filter Chroma
Technology notch filter > OD5 at both 376nm and 632.8nm < 95%
transmission 420-620nm, 650-750nm Fluorophore choice Nucleic acid
stain (Invitrogen Hoescht 34580) Mitochondria stain (Invitrogen
MitoTracker Deep Red)
Slide 62
Fluorescence imaging channel We have measured fluorescence of
films and active agents (UC-781, CSIC) Fluorescence can potentially
be used to simultaneously investigate the structure of these films
or the rate of diffusion of active agents Targeted Delivery of
PSC-RANTES for HIV-1 Prevention using Biodegradable Nanoparticles.
Ham, et al. Pharmaceutical Research, Vol. 26, No. 3, March
2009
Slide 63
System filters & fluorophores Bandpass filter Chroma
Technology notch filter > OD5 at both 376nm and 632.8nm < 95%
transmission 420-620nm, 650-750nm Fluorophore choice Nucleic acid
stain (Invitrogen Hoescht 34580) Mitochondria stain (Invitrogen
MitoTracker Deep Red)
Slide 64
Coregistered fluorescence & quantitative phase 10m
Fluorescence Map Red = Mitochondria Blue = Nuclei Quantitative
phase profile Phase Instantaneous Differential (PID) Blue= PID +
Red = PID -
Slide 65
Proposed Work Develop QPM instrumentation & techniques
Develop QPS instrumentation & techniques Apply QPM to dynamic
systems Apply QPS for molecular specificity Build InCh Microscope
Develop robust & standardized assay for film dissolution
Improve Illumination Add binocular display Use ratiometric imaging
& photothermal Image microbicidal films & RBCs with
molecular specificity