1
Generon solutions
for allergens detectionProduct Catalogue
2
In the modern industrial high throughput and
automated food processing, allergens are
becoming an increasingly important issue for
food safety.
Although the dose/response thresholds have
not yet been defined (as they may vary
among individuals), some individuals are so
sensitive to these allergens that even a
miniscule amount can cause serious problems
or even death.
In Europe, a norm concerning the control of
food allergens was issued in 2003 (Dir.
2003/89/CE) and later amended in 2006 (Dir.
2006/142/CE). The norm includes a list
(Annex IIIa) of ingredients that must be
compulsory pointed out in the label as they
can elicit allergic syndromes.
Food companies but also restaurants must
therefore aim for a real and effective control
of the allergen contamination through
convenient and reliable methods detecting
and n case quantifying allergens in order to
check effectiveness of cleansing procedures
and ingredients segregation. This will in turn
guarantee consumers about the trueness of
the labeling thus providing a real protection
of allergic individuals.
Allergens contamination may be revealed
through the specific detection either of the
allergen itself or of a marker indicating the
potential presence of the allergen, markers
include: specific proteins and/or DNA
fragments of the allergenic ingredient.
Although LC-MS is becoming popular in this
field, ELISA and PCR are the most diffused and
convenient methods for the detection and
quantification of food allergens as they
require inexpensive instruments and simple
laboratories setup. Both these methods have
analytical limits therefore, when possible,
their combined use is the most appropriate
approach according to scientific literature.
Thanks to the internal R&D activities and to
the collaboration with highly qualified
technical partners Generon offers clients a
comprehensive portfolio of products for the
detection of allergens including both
immunochemical and molecular kits. Generon
catalogue includes also various process
controls to increase reliability of the tests.
Catalogue Index
• Allergens Molecular Detection
• Ion-Force DNA extraction Page 3
• DNA extract Quantification Page 4
• SPECIALfinder™ Products Page 5
• Allergens Immunochemical Detection
• ELISA tests Page 11
• Lateral flow tests Page 15
• Laboratory Setup
• Equipment and Accessories Page 16
Summary
3
The sampling procedure determines the
“representativeness” of a result, whereas
quality and quantity of the analytes may vary
depending on the sample preparation.
Sampling and sample preparation are thus
crucial steps in the process of allergen
detection.
The limit of detection of the analytical
method as a whole is determined, not by the
most sensitive part of the procedure, but by
the least. In most cases this is the sample size.
A sample has to be representative of the
batch/lot of the product from which it was
taken, and the sample plan and sample size
have to meet statistical requirements with
respect to homogeneity and threshold limit
up to which the result should be reliable.
IonForce DNA Extractor is a kit planned to
maximize the yield and purity of the DNA
extracted from simple or complex matrixes
(rich in PCR inhibitors). It is an essential
system to obtain repeatable and reproducible
data in allergen molecular detection from
samples containing minimal traces of
contaminants.
• KEY Advantages
• 1-4-ALL APPROACH allows
extraction from swabs, vegetal or
animal matrices using the same kit
with specific protocols
• SCALABLE PROTOCOL allows
extractions from large and
representative sampling units
• FRAGMENTED DNA RECOVERY
allows extractions from processed
matrices
• PROGRESSIVE CLEAN-UP allows
removal of PCR inhibitors from
complex matrices
Ion-Force DNA Extraction
Core components
EXD001 Ion Force DNA extractor - 100 extractions
EXD010-P DNA Extraction Vacuum BOX
EXD010-A Adapters for Vacuum BOX (12 Pack)
ACC2908 Vacuum Pump for ION-Force
Ancillary Equipment for sample preparation phase
ACC2080 Benchtop Waring blender
ACC2525 200 ml PP sample containers
Ancillary Equipment for extraction phase
ACC2400 TS Heating-ThermoMixers, for 1 Thermoblock
ACC2402 TS Exchangeable thermoblock for 6x50 ml tubes
ACC2029 Water heating bath
ACC2507 15 ml PP tubes
ACC2508 50 ml PP tubes
ACC2907 Disposable syringe reservoirs ION-Force
ACC2906 Syringe disc filters for ION-Force
ACC2564 2.0 ml microtubes
ACC2905 Microtube rack
ACC2063 Microcentrifuge 0,5-2 ml tubes 14500 rpm
ACC2008 Benchtop Centrifuge fixed rotor for 50 ml tubes
ACC2007 15 ml tube adaptors for ACC2008
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The overall testing success in molecular
methods depends on DNA isolation. For many
reasons different matrices and also different
samples yield different amount of DNA
extract per gram. DNA Quantity defines
representation, and the contribution to
sampling error.
It’s therefore convenient to measure the
quantity of the DNA obtained after the
extraction and to normalize the quantity of
DNA loaded in a molecular reaction because
normalization:
• Provides uniformity in testing
• To comply with the sampling scheme
• To comply with validated process/assays
• Enhances robustness of testing, higher
success rate, hence, in the long run
reduces cost, and on the average may
improve turn around time
• Although qPCR enables in-assay
normalization, normalization allows for
semi-quantitative analysis.
DNA extract Quantification
Equipment and reagents for DNA Quantification
ACC040 Promega Quantus Fluorimeter
ACC040DS Quantus dsDNA reagent - 2000 Reactions
ACC040T Quantus 0,5 ml tubes - 5000 pcs
ACC2064 Palmtop spin-down centrifuge for tubes and PCR strips 7200 rpm
5
Real-Time PCR is the gold-standard for the
detection of DNA of contaminating
ingredients (like allergens) as it offers many
analytical advantages, for example: the
possibility to include a process control and/or
a reaction control; the possibility to detect
multiple allergens on the same DNA extract;
the possibility to detect the allergen even if
previously exposed to the cooking
temperatures of most food because DNA
typically remains intact.
In addition PCR-based allergens analyses
might include testing for presence of
amplifiable DNA from the particular species of
interest or from any amplifiable DNA from
plants or Eukaryotes as a further process
control.
SPECIALfinder is the portfolio of products for
the DNA based detection of allergens
developed by Generon that includes:
SPECIALfinder Assays - tests developed and
validated according to UNI-EN 15634-1: 2009
guidelines to detect the target allergen DNA
with high specificity and sensitivity. Absence
of cross-reactivity was tested towards a large
number of food ingredients to avoid false
positives. All the tests are made using prime-
quality molecular biology grade reagents.
Combining SPECIALfinder Assays to ION-
FORCE DNA Extractor, it’s possible to perform
allergens detection on any kind of matrix:
ingredients, semi-finished and finished
products environmental swabs.
SPECIALfinder DigiCount - total DNA
extracted from allergens using Generon ION-
Force DNA with SPECIALfinder Assays target
sequences content, quantified using Droplet
Digital PCR technology.
SPECIALfinder spyX - powdered allergen
dispersions contaminating corn flour at 200
ppm that can be used as analytical process
and extraction control with both molecular
and immunochemical methods.
Eventually, SPECIALfinder constitutes a turn-
key solution for demanding clients willing to
perform allergens control with a high level of
accuracy and confidence in the results.
SPECIALfinder™ Products
SPECIALfinder™ Products
• SPECIALfinder™ Assays Page 5
• SPECIALfinder™ DigiCount Page 7
• SPECIALfinder™ SpyX Page 8
6
Thanks to the internal R&D activities and to
the collaboration with highly qualified
technical partners Generon developed a
comprehensive portfolio of assays for
allergenic substances detection via PCR.
SPECIALfinder assays are extremely useful to
detect allergens in highly processed foods
because DNA is less prone to degradation
than proteins. Moreover, for celery, PCR is the
only tool to detect the allergen.
PCR is extremely specific and so the risk of
false positives is almost null. All the kits were
extensively tested against multiple food
ingredients to verify the absence of cross-
reactivity when working according to
instructions.
The DNA extracted from the matrices to be
controlled can be used to detect multiple
parameters, moreover the majority of the
tests share the same thermal profile, thus
SPECIALfinder allows for a very smooth and
convenient test procedure.
SPECIALfinder™ Screening Assays should be
paired with an appropriate polymerase mix
that contains an internal amplification control
(IAC) for identification of false-negative results
due to PCR inhibition, although when
performing amplifiability controls this is
redundant.
SPECIALfinder™ Assays
Mastermixes
Part # Description Amount
ENG002 GENERase PLUS Mastermix 100 Reactions
ENG002-50 GENERase PLUS Mastermix 50 Reactions
Complementary polymerases to assays sold separately. GENERase PLUS includes the internal amplification control to be detected in
the HEX fluorophore channel.
Assays
Part # Description Amount
PAV01A SPECIALfinder Soy (soia) 100 Reactions
PAV01A-50 SPECIALfinder Soy (soia) 50 Reactions
PAV02A SPECIALfinder Peanut (arachide) 100 Reactions
PAV02A-50 SPECIALfinder Peanut (arachide) 50 Reactions
PAV03A SPECIALfinder Hazelnut (nocciola) 100 Reactions
PAV03A-50 SPECIALfinder Hazelnut (nocciola) 50 Reactions
PAV05A SPECIALfinder Pecan Nut (noce Pecan) 100 Reactions
PAV05A-50 SPECIALfinder Pecan Nut (noce Pecan) 50 Reactions
PAV06A SPECIALfinder Walnut (noce) 100 Reactions
PAV06A-50 SPECIALfinder Walnut (noce) 50 Reactions
PAV07A SPECIALfinder Pistachio Nut (pistacchio) 100 Reactions
PAV07A-50 SPECIALfinder Pistachio Nut (pistacchio) 50 Reactions
PAV08A SPECIALfinder Cashew (anacardo) 100 Reactions
PAV08A-50 SPECIALfinder Cashew (anacardo) 50 Reactions
PAV09A SPECIALfinder Almond (mandorla) 100 Reactions
PAV09A-50 SPECIALfinder Almond (mandorla) 50 Reactions
PAV10A SPECIALfinder Rye (segale) 100 Reactions
PAV10A-50 SPECIALfinder Rye (segale) 50 Reactions
con/nue→
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SPECIALfinder™ Assays
Assays
Part # Description Amount
PAV11A SPECIALfinder Oats (avena) 100 Reactions
PAV11A-50 SPECIALfinder Oats (avena) 50 Reactions
PAV13A SPECIALfinder Sesame (sesamo) 100 Reactions
PAV13A-50 SPECIALfinder Sesame (sesamo) 50 Reactions
PAV15A SPECIALfinder Celery (sedano) 100 Reactions
PAV15A-50 SPECIALfinder Celery (sedano) 50 Reactions
PAV16A SPECIALfinder Brazil nut (noce brasiliana) 100 Reactions
PAV16A-50 SPECIALfinder Brazil nut (noce brasiliana) 50 Reactions
PAV20A SPECIALfinder Fish (pesce) 100 Reactions
PAV20A-50 SPECIALfinder Fish (pesce) 50 Reactions
PAV21A SPECIALfinder Macadamia Nut (macadamia) 100 Reactions
PAV21A-50 SPECIALfinder Macadamia Nut (macadamia) 50 Reactions
COMBOScreen Boxes
Part # Description Amount
PAV00C SPECIALfinder – Combo Kit - 4xTargets (25 Rx/Target) 100 Reactions
Convenient boxes containing multiple assays on customer choice (to be selected among the target listed above). For evaluationpurposes only.
Generic Amplifiability Target
Part # Description Amount
PGE06A MODIfinder 28S Assay 100 Reactions
PGE06A-50 MODIfinder 28S Assay 50 Reactions
PGE08A MODIfinder Chloroplast Endo Assay 100 Reactions
PGE08A-50 MODIfinder Chloroplast Assay 50 Reactions
All the assays allows for qualitative DNA detection with a detection limit of 5 genomic copies depending on matrix and DNApreparation. Reaction volume 20 µl (sample 5 µl). Each assay box contains a positive and negative control.
SPECIALfinder assays for Lupine (lupino) - Barley (orzo) - Mustard (senape) - Gluten (glutine) - Crustaceans (crostacei) - Shellfish(molluschi) are in development.
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SPECIALfinder™ DigiCount
Standard Solutions Catalog
Part # Description Amount
PAV01R SPECIALfinder DigiCount Soy (soia) 1 Vial
PAV02R SPECIALfinder DigiCount Peanut (arachide) 1 Vial
PAV03R SPECIALfinder DigiCount Hazelnut (nocciola) 1 Vial
PAV05R SPECIALfinder DigiCount Pecan Nut (noce Pecan) 1 Vial
PAV06R SPECIALfinder DigiCount Walnut (noce) 1 Vial
PAV07R SPECIALfinder DigiCount Pistachio Nut (pistacchio) 1 Vial
PAV08R SPECIALfinder DigiCount Cashew (anacardo) 1 Vial
PAV09R SPECIALfinder DigiCount Almond (mandorla) 1 Vial
PAV10R SPECIALfinder DigiCount Rye (segale) 1 Vial
PAV11R SPECIALfinder DigiCount Oats (avena) 1 Vial
PAV13R SPECIALfinder DigiCount Sesame (sesamo) 1 Vial
PAV15R SPECIALfinder DigiCount Celery (sedano) 1 Vial
PAV16R SPECIALfinder DigiCount Brazil nut (noce brasiliana) 1 Vial
PAV20R* SPECIALfinder DigiCount Fish (pesce) 1 Vial
PAV21R SPECIALfinder DigiCount Macadamia Nut (macadamia) 1 Vial
Target DNA extracted from certified material using ION-Force DNA Extraction kit, quantified using ddPCR and the respective
SPECIALfinder assay. Each vial contains 100 µL of standardized buffered solution of eukaryotic DNA at 100 copies/µL.
(*) By default the DNA is extracted from cod, other species available on request.
The basis of digital PCR (dPCR) is to quantify
the absolute number of targets present in a
sample, using limiting dilutions, PCR and
Poisson statistics. To do so, the PCR mix is
distributed across a large number of
partitions containing zero, one or more copies
of the target nucleic acid. After end-point PCR
amplification, each partition is scrutinized and
defined as positive (‘‘1’’, presence of PCR
product) or negative (‘‘0’’, absence of PCR
product) hence the term ‘‘digital’’. The
absolute number of target nucleic acid
molecules contained in the original sample
before partitioning can be calculated directly
from the ratio of positive to total partitions,
using binomial Poisson statistics.
Digital PCR is an alternative technique for
quantifying gene copy number which may
provide more accurate measurements than
other approaches currently available as it is
not dependant on amplification efficiency. Of
particular importance, ddPCR measurements
are made independent of any calibrator and,
therefore, this technique has the potential to
be considered as a primary method for use in
certification of nucleic acid reference
materials.
The measurement principle has a high
metrological quality and can overcome
several of Real-Time GMO analysis difficulties
by transforming exponential data from
conventional PCR to digital signals that simply
indicate whether or not amplification has
occurred after a defined number of cycles.
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SPECIALfinder™ SpyX
Spiked matrix catalog
Part # Description Amount
PAV01X SPECIALfinder spyX Soy (soia) 1 Tube
PAV02X SPECIALfinder spyX Peanut (arachide) 1 Tube
PAV03X SPECIALfinder spyX Hazelnut (nocciola) 1 Tube
PAV04X SPECIALfinder spyX Wheat (frumento) 1 Tube
PAV06X SPECIALfinder spyX Nut (noce) 1 Tube
PAV07X SPECIALfinder spyX Pistachio (pistacchio) 1 Tube
PAV08X SPECIALfinder spyX Cashew (anacardo) 1 Tube
PAV09X SPECIALfinder spyX Almond (mandorla) 1 Tube
PAV10X SPECIALfinder spyX Rye (segale) 1 Tube
PAV11X SPECIALfinder spyX Oats (avena) 1 Tube
PAV13X SPECIALfinder spyX Sesame (sesamo) 1 Tube
PAV15X SPECIALfinder spyX Celery (sedano) 1 Tube
PAV16X SPECIALfinder spyX Brazil nut (noce brasiliana) 1 Tube
PAV18X SPECIALfinder spyX Lupin (lupino) 1 Tube
PAV20X SPECIALfinder spyX Fish (pesce) 1 Tube
PAV21X SPECIALfinder spyX Macadamia Nut (macadamia) 1 Tube
PAV05X SPECIALfinder spyX Pecan Nut (noce Pecan) 1 Tube
PAV12X SPECIALfinder spyX Barley (orzo) 1 Tube
PAV14X SPECIALfinder spyX Mustard (senape) 1 Tube
PAV19X SPECIALfinder spyX Crustaceans (crostacei) 1 Tube
Each 50 ml tube contains 200 ppm (mg/kg) of freeze-dried allergen dispersed in 5 g of corn flour. Customizations are available on
demand
Process control is defined as an activity
involved in ensuring a process is predictable,
stable, and consistently operating at the
target level of performance with only normal
variation.
The importance of process control is often
undervalued in both molecular biology (as
attention was mainly oriented toward the
primers&probe design) and immunochemistry
(focus on antibody only).
In both these technical approaches the
number of steps composing the process
cannot be reduced just to the detection and
the matrix has a deep impact in the outcome
of the analysis.
To address this topic Generon developed SpyX
– freeze dried and powdered allergens
dispersed in corn flour to be processed as
unknown samples or further dispersed to
validate analytical processes on specific
matrices.
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Allergens Immunochemical Detection
Food allergies affect an estimated 2 to 4% ofthe population around the world, often withrisks of severe effects, thus making foodallergies a serious concern for public healtharound the world. While declaration ofingredients derived from allergenic sourceswhen used as direct ingredients or processingaids has helped to protect allergic consumerscross-contact can occasionally occur despitethe food manufacturer’s best efforts to avoidit. The food industry strives to mitigate thisrisk through use of allergen control plans andvalidated cleaning and sanitation procedures.
Although information on minimum elicitingdoses of allergic individuals has emerged forvarious food allergens in recent years, there isstill little consensus in the scientificcommunity about it. Therefore currentlymany countries have not implementedregulatory thresholds. Japan currentlyrequires labeling when the concentration ofprotein from the allergenic source is > 10ppm.
With the lack of regulatory thresholds, foodindustry is tasked with complying withessentially a zero threshold level of allergenicresidue. This is operationally impossible giventhe complexity of manufacturing facilities and
the numerous routes of allergencontamination and cross-contact that canoccur throughout the supply chain.
Analytical validation to ensure removal ofallergenic residue from equipment surfaces orto ensure that the finished product does notcontain the allergenic residue of concern isutilized by the food industry to minimize therisk of hidden allergens in the products.
There are numerous quantitative andqualitative methods that are available formonitoring residues from allergenic sources.It is quite important for food manufacturers tounderstand the advantages and limitations ofthe available analytical tools when selectingan appropriate method to ensure that theanalytical results provide meaningful datathat can be used for risk managementpurposes.
Immunochemical methods are a broadclassification of analytical methods used forthe detection of allergenic food residues.These methods rely on binding of specificantibodies to the allergenic food protein to bedetected or to a protein typical of theallergenic substance.
Products
• ELISA kits Page 10
• Gluten detection Page 11
• Other allergens Page 12
• Histamine detection Page 13
• Lateral Flow kits Page 14
11
Enzyme-linked immunosorbent assays
(ELISAs) are the methods most widely used by
the food industry for detecting specific
allergenic protein.
ELISAs provide several advantages including:
• They detect protein(s) from the allergenic
source of interest, which make these
assays ideal for validation of the removal of
specific allergenic proteins
• The readout is obtained by the
development of a visible color thus
allowing either a quantitative analysis
through a inexpensive spectrophotometer
or a semi-quantitative measurement
through a visual evaluation.
• They are sufficiently sensitive to ensure the
safety of the allergic consumer (detection
limits generally range in the low milligram
per kilogram [ppm] range)
• The reagents used in the assay are suited
for use within the food processing facility
• They provide a rapid assessment that can
be run in the food processing facility or in a
food industry laboratory.
Quantitative ELISAs can be developed in the
sandwich or competitive formats. The
sandwich ELISA is the most common format
used for detection of food allergens; the
intensity of the color is proportional to the
concentration of allergen present in the
sample.
Quantification can be accomplished by
comparing the absorbance of each sample to
the absorbance of a standard curve.
In competitive ELISA any allergenic protein
present in the sample will competitively
inhibit binding of the antibodies so color
intensity is inversely proportional to the
concentration of the allergen present in the
sample. One advantage of competitive ELISA
is that it allows detection of fermented or
hydrolyzed proteins where the allergenic
proteins may be partially digested.
Generon offers a comprehensive panel of
ELISA kits to detect allergens in swab and food
products thanks to the collaboration with
international partners like Elution
Technologies, Ingenasa and LDN which where
selected after an extensive evaluation of their
kit performances in our R&D laboratory.
Generon provides also a complete pre- and
post-sales support to bring the best from
these analytical tools through on-site
trainings.
Our catalogue also include all the ancillary
equipment to perform the experiments and
process controls (see SPECIALfinder spyX @
page 8) to verify their correct execution.
Allergens ELISA Detection
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Gluten ELISA Detection
The term “gluten” refers to a specific complex
of proteins found in the endosperm of some
cereal grains (e.g. wheat, rye, and barley). It is
the group of proteins that give elasticity to
dough, allowing it to rise or give a chewy
texture to baked goods.
Celiac disease is a chronic inflammatory
disorder of the small intestine in genetically
susceptible individuals. It is triggered by
ingesting gluten.
As many as 1% of the general population in
the world will develop celiac disease, while
the prevalence is up to 10% for those with an
affected immediate family member. Although
celiac disease has no known cure, individuals
who have this disease are advised to avoid all
sources of gluten in their diet. Over time,
strictly avoiding consumption of all gluten
sources can improve the symptoms and
reduce the associated health risks of celiac
disease.
Gluten sensitivity is an inflammatory
condition caused by dietary gluten in the
intestines. This is distinct from celiac disease
in that it is not an autoimmune disorder and
there is no simple antibody test for this
condition. It is difficult to estimate the
prevalence of this condition due to a high rate
of self-diagnosis, it’s estimated that gluten
sensitivity affects 6% of the population. The
only reliable treatment is the elimination of
gluten from the diet.
Both EFSA and FDA issued a final rule that
defines what characteristics a food has to
have to bear a label that proclaims it “gluten-
free.” The rule also holds foods labeled
“without gluten,” “free of gluten,” and “no
gluten” to the same standard. The limit of
tolerated contamination was set at 20 ppm.
Generon supplies Ingenasa R5 based ELISA
kits compliant to the indications of the CODEX
Alimentarius.
Ingenasa Kits for the ELISA detection of gluten
Part # Description
LFD030-Q Ingezim Gluten kit - Quick: R5 antibody based detection Sandwich ELISA kit to quantify prolamines from
wheat, rye and barley in food declared as gluten-free. Detection limit: 1.5 mg/kg (ppm) gliadin or 3.0 mg/kg
(ppm) gluten. The kit includes an extraction buffer β-mercaptoethanol Free and 5 standard points for
calibration curve. 96 determinations in 12x8 well precoated strips.
LFD031 Ingezim semiQ Gluten Kit: R5 antibody based detection Sandwich ELISA kit to visually detect presence of
gluten over a precautional threshold of 10 ppm. 32 Determinations.
LFD053 Ingezim Hydrolyzed Gluten kit: R5 competitive ELISA to quantify potential toxic peptide sequences from
prolamines from wheat, rye and barley in fermented and hydrolyzed food (e.g. beer, starch, starch syrup, malt
extracts); sample preparation with a dedicated patented buffer. Detection limit: 0.25 mg/kg (ppm) hydrolyzed
gluten. 48 Determinations in 6x8 wells precoated strips.
MCE024 Gluten Extraction Cocktail Solution with β-mercaptoethanol 1000 ml
MCE024-1 Gluten Extraction Cocktail Solution with β-mercaptoethanol 100 ml
MCE024-5 Gluten Extraction Cocktail Solution with β-mercaptoethanol 500 ml
Elution Technologies ELISA kits
Part # Description Amount Assay Range
E-75ALM (E-75ALM-48) Almond Protein ELISA Kit 96 (or 48) Determinations 1.0 – 27 ppm
E-75CAS (E-75CAS-48) Bovine Casein ELISA Kit 96 (or 48) Determinations 0.5 – 12 ppm
E-75MLK (E-75MLK-48) Bovine Total Milk ELISA Kit 96 (or 48) Determinations 1.0 – 81 ppm
E-75BZL (E-75BZL-48) Brazil Nut Protein ELISA Kit 96 (or 48) Determinations 1.0 – 27 ppm
E-75CSH (E-75CSH-48) Cashew Protein ELISA Kit 96 (or 48) Determinations 0.9 – 24.3 ppm
E-75CNT (E-75CNT-48) Coconut Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
E-75RST (E-75RST-48) Crustacean Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
E-75EOM (E-75EOM-48) Egg White Protein ELISA Kit 96 (or 48) Determinations 0.5 – 13.5 ppm
E-75FSH (E-75FSH-48) Fish Protein ELISA Kit 96 (or 48) Determinations 1.0 – 27 ppm
E-75HZL (E-75HZL-48) Hazelnut Protein ELISA Kit 96 (or 48) Determinations 1.0 – 81 ppm
E-75MCD (E-75MCD-48) Macadamia Nut Protein ELISA Kit 96 (or 48) Determinations 0.3 – 27 ppm
E-75MSK (E-75MSK-48) Mollusk Protein ELISA Kit 96 (or 48) Determinations 1.0 – 27 ppm
E-75MST (E-75MST-48) Mustard Protein ELISA Kit 96 (or 48) Determinations 1.5 – 40.5 ppm
E-75PNT (E-75PNT-48) Peanut Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
E-75PCN (E-75PCN-48) Pecan Protein ELISA Kit 96 (or 48) Determinations 0.7 - 54 ppm
E-75PNE (E-75PNE-48) Pine Nut Protein ELISA Kit 96 (or 48) Determinations 1.5 – 40.5 ppm
E-75SME (E-75SME-48) Sesame Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
E-75SOY (E-75SOY-48) Soy Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
E-75WNT (E-75WNT-48) Walnut Protein ELISA Kit 96 (or 48) Determinations 2.0 – 54 ppm
Ingenasa ELISA kits
Part # Description Amount Assay Range
LFD032 Ingezim β-lattoglobulin Kit 48 Determinations 0.1 – 0.8 ppm
LFD054 Ingezim Casein Kit 48 Determinations 0.2 – 2.0 ppm
LFD055 Ingezim Ovoalbumin Kit 48 Determinations 0.2 – 2.0 ppm
13
For the detection of all but gluten allergens
Generon relies on the products of Elution
Technologies a US company born through the
collaboration between a major service lab
(BIA Diagnostics) specialized in allergens
detection and a manufacturer of antibodies
(ICL) in response to industry demand for new
and improved detection methods for food
allergens. The unique capabilities of BIA and
ICL allow Elution Technologies to be at the
forefront of this business and provide
unmatched technical support on products.
Both for Elution Technologies and Generon
the needs of our customers are the focus of
all our activities that’s why we partnered, to
build lasting and mutually profitable
relationships with our clients. Generon offers
training on these products in its own demo
laboratory or on-site.
Other Allergens Detection
14
Histamine Detection
Histamine is a product of decomposition of
histidine caused by the growth of certain
bacteria in seafood. Fish containing high levels
of histamine has been associated with many
examples of poisoning commonly referred to
as “scombroid poisoning,” a major health
problem for consumers.
Glutamate is one of the most abundant
naturally occurring non-essential amino acids.
It is widely used by food manufacturers as a
food additive to enhance the flavor. Under
normal conditions, humans have the ability to
metabolize glutamate that has a very low
acute toxicity. But sensitive individuals may
experience transient adverse reactions such
as headache, dizziness, flushing, and
generalized weakness – a symptom complex
which is termed Chinese Restaurant
Syndrome.
Generon distribute in Italy LDN´s assays based
on ELISA for the quantitative and/or semi-
quantitative determination of Histamine and
glutamate.
LDN Kits for the ELISA detection of histamine and glutamate
Part # Description
LFD047 HistaSure™ ELISA High Sensitivity Kit: immunoassay (ELISA) for the quantitative determination
of Histamine in fish meal, fresh fish, canned fish, sausage, cheese, milk and wine in less than 90
minutes. In a comparative study where different commercial Histamine test kits had been compared the
HistaSure™ ELISA High-Sensitive showed the best correlation to the HPLC-method and the authors concluded
that this kit “is found to be a reliable method for testing histamine in TFPs (traditional food
products)either for regulatory or HACCP applications in monitoring such hazard”.
Detection limit: 0.15 µg/l (ppb). 96 determinations in 12x8 well precoated strips.
LFD048 HistaSure™ ELISA Fast Track Kit: immunoassay (ELISA) for the quantitative or semi-quantitative determination
of Histamine in fish and fish-derived products providing results in less than 30 minutes. Validated and certified
by the AOAC Research Institute for many different fish species and matrices such as fresh/frozen yellowfin
tuna, canned tuna-chunk light in water, fresh/frozen mahimahi, canned sardines in oil and fish meal.
Detection range 0.44 – 300 ppm. 48 determinations in 6x8 well precoated strips.
LFD049 HisQuick™ Photometric Kit: rapid colorimetric assay for the quantitative or semi-quantitative determination
of Histamine in fish meal in less than 15 minutes. Easy sample preparation suitable for “in-field” analysis.
Detection range 125 - 2 000 ppm. 48 determinations 12x8 break apart wells microtiter plate
LFD052 Glutamate Food ELISA Kit: immunoassay (ELISA) for the quantitative determination of Glutamate in food
in less than 3 hours.
Detection range 0.3 - 60 µg/ml. 96 determinations 12x8 break apart wells microtiter plate
15
Lateral Flow Kits
Lateral flow assays (LFAs) are a qualitative
immunochromatographic form of an ELISA.
These assays are also referred to as lateral
flow dipsticks (LFDs) and works just like a
home pregnancy test.
An extracted food sample, swab, or final rinse
water sample is first applied to the sample
filter area. If present, the allergenic proteins
of interest will bind to the coupled antibody
and continue to wick through the LFA by
capillary action. The coupled antibody-
allergen (if present in the sample) will migrate
to the test zone, and the coupled allergen will
bind to the antibodies present in this zone,
forming a visible line that indicates the
positive presence of the specific allergen of
interest.
The intensity of the line can be correlated to
the concentration of the allergen present in
the sample. Semi-quantitative results can be
obtained if a strip reader is utilized.
In 10 minutes these rapid tests for portable
onsite monitoring of specific food allergens
accurately detects both processed and
unprocessed proteins. Although they can be
used for finished products they provide major
advantages in the analysis of CIP, swabbing,
and raw ingredients when time to result is
fundamental.
All Elution Technologies LFDs comes complete
of all the necessary disposable for analysis
including 25 swabs.
Product Catalogue
Part # Description Amount Cut-Off
ALMD-1007 Almond Protein Rapid Test 25 LFD 2 ppm
MILK-1004 Bovine Total Milk Rapid Test 25 LFD 2 ppm
CASH-1013 Cashew Protein Rapid Test 25 LFD 2 ppm
COCO-1011 Coconut Protein Rapid Test 25 LFD 2 ppm
BEGG-1003 Egg Protein Rapid Test 25 LFD 1 ppm
FISH-1014 Fish Protein Rapid Test 25 LFD 2 ppm
GLTN-1005 Gluten Rapid Test 25 LFD 5 ppm
PNT-1017 Peanut Protein Rapid Test 25 LFD 2 ppm
PCN-1016 Pecan Protein Rapid Test 25 LFD 2 ppm
PIST-1006 Pistachio Protein Rapid Test 25 LFD 2 ppm
BSOY-1002 Soy Protein Rapid Test 25 LFD 2 ppm
WLNT-1015 Walnut Protein Rapid Test 25 LFD 2 ppm
LFD050 LDN HistaSure™ Fish Rapid Test for Histamine 24 LFD 50 ppm
16
Equipment and Accessories
A certain number of ancillary equipment and
lab commodities (mainly plasticware) are
required to perform the analysis although in
many cases a part of the plasticware is
included in the kits.
Generon portfolio includes also these items in
order to provide the customer a turn-key
solution for allergen testing, whatever the
detection method selected is.
Ancillary equipment for allergens detection using molecular biology
Part # Description Amount
ACC2016 Variable Volume Micropipette 2-20 µl 1 Unit
ACC2015 Variable Volume Micropipette 20-200 µl 1 Unit
ACC2014 Variable Volume Micropipette 100-1000 µl 1 Unit
ACC2011 Multichannel micropipette 8x 0.5-10 µl 1 Unit
ACC2516 Micropipette tips 2-20 µl with filter 96 tips box
ACC2514 Micropipette tips 100-1000 µl with filter 96 tips box
ACC2515 Micropipette tips 20-200 µl with filter 96 tips box
ACC2017 Micropipette rack 1 Unit
ACC2038 Balance 100g - 0.001g precision 1 Unit
ACC2039 Balance 1000g - 0.1g precision 1 Unit
ACC2080 Benchtop Waring blender 1 Unit
ACC2525 200 ml PP sample containers 250
ACC2064 Palmtop spin-down centrifuge for tubes and PCR strips 7200 rpm 1 Unit
ACC2063 Microcentrifuge 0,5-2 ml tubes 14500 rpm 1 Unit
ACC2564 2.0 ml microtubes 1000
ACC2905 Microtube rack 1 Unit
ACC2507 15 ml PP tubes 500 Units
ACC2508 50 ml PP tubes 500 Units
ACC2909 15 ml tube rack 1 Unit
ACC2509 50 ml tube rack 1 Unit
ACC2003 Variable speed vortex 1 Unit
ACC2095 Benchtop PCR hood 1 Unit
ACC2008 Benchtop Centrifuge fixed rotor for 50 ml tubes 1 Unit
ACC2007 15 ml tube adaptors set for ACC2008 1 Unit
ACC2908 Vacuum Pump for ION-Force 1 Unit
ACC2300 DB Heating-ThermoMixers, for 1 Thermoblock 1 Unit
ACC2301 DB Heating-Thermomixer for 2 Thermoblocks 1 Unit
ACC2302 DB Exchangeable thermoblock for 6x50 ml tubes 1 Unit
ACC2303 DB Exchangeable thermoblock for 2 ml tubes 1 Unit
ACC2400 TS Heating-ThermoMixers, for 1 Thermoblock 1 Unit
ACC2402 TS Exchangeable thermoblock for 6x50 ml tubes 1 Unit
ACC2403 TS Exchangeable thermoblock for 2 ml tubes 1 Unit
ACC2000 Notebook PC 1 Unit
BR3594990 Industrial Diagnostics CFX96™ DeepWell Real-Time PCR Detection System 1 Unit
BR3593995 Industrial Diagnostics MiniOpticon™ Real-Time PCR Detection System 1 Unit
17
Equipment and Accessories
Ancillary equipment for allergen detection using ELISA
Part # Description Amount
ACC1028 ELISA microplate reader 1 Unit
ACC1000 Notebook PC 1 Unit
ACC1030 Orbital microplate shaker 1 Unit
ACC1027 Automatic microplate washer 1 Unit
ACC1004 1000 ml Bottle for washing buffer preparation 1 Unit
ACC1505 1000 ml graduated cylinder 1 Unit
ACC1506 100 ml graduated cylinder 1 Unit
ACC1015 Variable Volume Micropipette 20-200 µl 1 Unit
ACC1014 Variable Volume Micropipette 100-1000 µl 1 Unit
ACC1018 Multichannel micropipette 8x 30-300 µl 1 Unit
ACC1021 Reservoir for multichannel pipette 100 Units
ACC1514 Micropipette tips 100-1000 µl 96 Tips Box
ACC1515 Micropipette tips 20-200 µl with filter 96 Tips Box
ACC1017 Micropipette rack 1 Unit
ACC1019 Multistep pipette 1 Unit
ACC1020 Multistep pipette syringe non-sterile 1 Bag
ACC1039 Balance 1000g - 0.1g precision 1 Unit
ACC1080 Benchtop Waring blender 1 Unit
ACC1525 200 ml PP sample containers 250 Units
ACC1912 Orbital shaker for containers 1 Unit
ACC1504 200 mm diameter pre-plied paper filters 100
ACC1064 Palmtop spin-down centrifuge 7200 rpm 1 Unit
ACC1564 2.0 ml microtubes 1000 Units
ACC1905 Microtube rack 1 Unit
ACC1003 Variable speed vortex 1 Unit
Ancillary equipment for allergen detection using lateral flows
Part # Description Amount
ACC3506 100 ml graduated cylinder 1 Unit
ACC3017 Micropipette rack 1 Unit
ACC3039 Balance 1000g - 0.1g precision 1 Unit
ACC3080 Benchtop Waring blender 1 Unit
ACC3525 200 ml PP sample containers 250 Units
ACC3912 Orbital shaker for containers 1 Unit
18
Contacts and Addresses
International Contacts
Mr. Nicola Bortoletto [email protected] Mobile +39 346 5995589
Mrs. Caterina Novi [email protected] Mobile +39 345 6581422
Mr. Mustafa Aguezzoul [email protected] Mobile +33 65 2822140
Mr. Enrico Arletti [email protected] Mobile +39 348 4227992
Stradello Aggazzotti,104 – Modena - � +39 059 8637161� +39 059 8637162
� [email protected] � www.generon.it
Responsabili commerciali di zona in Italia
Piemonte, Liguria e VDA Stefano Callea - [email protected] +39 349 5503780
Lombardia Daniela Superchi - [email protected] +39 347 5644144
Triveneto Alessandro Ruffato - [email protected] +39 349 0651399
Simone Ruffato - [email protected] +39 349 5291261
Emilia Romagna, Marche, Toscana Francesco Rossi - [email protected] +39 344 2685914
Puglia e Basilicata Vito Lorusso - [email protected] +39 328 6274587
Altre regioni Caterina Novi - [email protected] +39 345 6581422
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