Image J
Sample Pictures Available athttp://biology2020.wikispaces.com/
Onion
Find your file and open it.
Select the straight line tool. Drag across your field of view.
Under analyze: Set scale
Pixels are already there. Enter your known distance.
Trace a cell wall with the free hand tool (before).
Go to analyze: Measure
Analyze: Set Measurements
Measure DNA Distance
Leaf Surface Area
Compare Stomata per surface area
Oak 400X Rose 400X
Choose the Multi point tool (right click)
Click to number(21 in the field of view)
• Diameter= 0.45 mm• Radius= 0.225 mm• Area of FOV= 0.145mm2 (from Image J)• Verify ?