SEPPIM S.A.S.
ZONE INDUSTRIELLE 61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51
DIAGNOSTIC REAGENTS
MANUAL METHODS
Version : MMAN-09 April 2010
SEPPIM S.A.S.
ZONE INDUSTRIELLE 61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51
REAGENTS
A Version
ACID PHOSPHATASE .................................MM-PACI-1 ALBUMIN .................................................. MM-ALBU-1
ALP (DEA) SL .............................................. MM-PASL-1 ALP (DEA) ................................................... MM-PALC-1
ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1 ALT/GPT ..................................................... MM-ALAT-2
AMYLASE SL ............................................. MM-AMSL-3 AST/GOT 4+1 SL .................................... MM-ASSL4/1-1 AST/GOT ..................................................... MM-ASAT-2
B
BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2
C
CALCIUM ARSENAZO ............................. MM-CALA-1 CALCIUM OCPC ........................................ MM-CALO-1
Update CHLORIDE ................................................. MM-CHLO-2
CHOLESTEROL SL ..................................... MM-CHSL-2 Update CHOLESTEROL ......................................... MM-CHOL-3 CHOLESTEROL HDL SL 2G .................... MM-HDLL-1 CHOLESTEROL LDL SL 2G ...................... MM-LDLL-1 HDL CHOLESTEROL ................................ MM-HDLC-2 CHOLINESTERASE .................................... MM-CHES-2
CK MB SL ................................................... MM-CMSL-2 CK MB ........................................................ MM-CKMB-2
CK NAC SL .................................................. MM-CKSL-2 CK NAC ....................................................... MM-CKNA-2 COPPER........................................................ MM-CUIV-1
CREATININE JAFFE .................................. MM-CRCO-1 CREATININE PAP SL ................................. MM-CRSL-3
G Version GAMMA GT SL ........................................... MM-GASL-1 GAMMA GT ............................................... MM-GAGT-1
GLUCOSE HK SL ........................................ MM-GHSL-1 GLUCOSE PAP SL ...................................... MM-GPSL-2 GLUCOSE PAP ............................................ MM-GLUP-1
I IRON CHROMAZUROL ............................. MM-FECA-1 IRON FERROZINE ................................... MM-FEFR-2
L
LACTATE .................................................... MM-LACT-2
New LDH-L SL ......................................................MM-LLSL-1 LDH-P .......................................................... MM-LDHP-2
M
MAGNESIUM CALMAGITE ................... MM-MAGN-1 MICROPROTEIN ........................................ MM-PRTP-2
P
PHOSPHORUS ............................................ MM-PHOS-1
T TOTAL PROTEIN PLUS ............................. MM-PROB-1 TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2 TRIGLYCERIDES ........................................ MM-TRIG-2
U
UREA UV SL ............................................... MM-URSL-2 UREA UV .................................................... MM-URUV-1
URIC ACID ................................................. MM-ACUR-2 URIC ACID MONO SL .............................. MM-AUML-2 Update URIC ACID SL ............................................ MM-AUSL-2
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ACID PHOSPHATASE
Ref. : PACI- 0030 18 x 3 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and after a 5 minute incubation measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION Total ACP : Activity (U/L) = OD/min. x 853 NP ACP : Activity (U/L) = OD/min. x 860 Prostatic fraction (U/L) = Total ACP (U/L) - NP ACP (U/L). LINEARITY If OD/min. exceeds 0.070 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-PACI-1
Total acid phosphatase Non-prostatic acid phosphataseWorking Reagent 1 1 mL 1 mL Working Reagent 2 10 L Sample 100 L 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ALBUMIN
Ref.: ALBU - 0600 2 x 125 mL ALBU - 0700 4 x 250 mL
MANUAL METHOD
PROCEDURE Wavelength : 628 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 15 minutes. CALCULATION OD Sample x n OD Standard n = standard concentration
NOTE: For more details, see the Instructions for use
(07/2005) MM-ALBU-1
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ALP (DEA) SL
Rf. : PASL- 0400 2 x 62.5 mL PASL- 0420 4 x 62.5 mL PASL- 0500 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm (410) Temperature : 37C, 30C, 25C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION One reagent procedure Two reagents procedure At 405 nm: Activity (U/L) = OD/min. x 2 750 3424 At 410 nm: Activity (U/L) = OD/min. x 2 910 3623 LINEARITY If OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-PASL-1
Working reagent : 1 mL Sample : 20 L
R1 : 1 mL Sample : 20 L
R2 : 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ALP (DEA)
Rf. : PALC- 0030 20 x 3 mL PALC- 0200 12 x 20 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm (410) Temperature : 25C, 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 405 nm: Activity (U/L) = OD/min. x 2 750 At 410 nm: Activity (U/L) = OD/min. x 2 910 LINEARITY If OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-PALC-1
Working reagent : 1 mL Sample : 20 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ALT/GPT 4+1 SL
Ref.: ALSL 0410 2 x 62.5 mL ALSL 0430 4 x 62.5 mL ALSL 0510 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 1 746 LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-ALSL4/1-1
Working reagent : 1 mL Sample : 100 L
R1 : 1 mL Sample : 125 L
R2 : 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
ALT/GPT
Ref.: ALAT - 0200 12 x 20 mL ALAT - 0400 9 x 50 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm (334 365) Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = OD/min. x 1 746 At 334 nm: Activity (U/L) = OD/min. x 1 780 At 365 nm: Activity (U/L) = OD/min. x 3235 LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(03/2008) MM-ALAT-2
Working reagent : 1 mL Sample : 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
AMYLASE SL
Rf. : AMSL - 0390 1 x 50 mL AMSL - 0400 6 x 50 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION 37 C 30 C At 405 nm: Activity (U/L) = OD/min. x 4640 2812 Take dilution factor into account for the calculation of the activity in urine. LINEARITY Up to 900 U/L If OD/min. exceeds 0.190 at 405 nm, repeat test using sample diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(11/2006) MM-AMSL-3
37 C 30 C Reagent 1 mL 1 mL Sample 15 L 25 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
AST/GOT 4+1 SL
Rf. : ASSL- 0410 2 x 62.5 mL ASSL- 0430 4 x 62.5 mL ASSL- 0510 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 1 746 LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-ASSL4/1-1
Working reagent : 1 mL Sample : 100 L
R1 : 1 mL Sample : 125 L
R2 : 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
AST/GOT
Ref.: ASAT - 0200 12 x 20 mL ASAT - 0400 9 x 50 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm (334 365) Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = OD/min. x 1 746 At 334 nm: Activity (U/L) = OD/min. x 1 780 At 365 nm: Activity (U/L) = OD/min. x 3235 LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2008) MM-ASAT-2
Working reagent : 1 mL Sample : 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
BILIRUBIN
TOTAL & DIRECT 4+1
Ref.: BITD 0600 Total 125 mL Direct 125 mL
BITO - 0600 Total 2 x 125 mL
BIDI - 0600 Direct 2 x 125 mL
MANUAL METHOD
PROCEDURE for TOTAL BILIRUBIN
Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path
Read against distilled water.
CALIBRATION TEST
R1 BILIRUBIN TOTAL 4+1 800 L 800 L
Calibrator 100 L
Sample 100 L
Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD1 OD2
Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 L 200 L
Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD3 OD4
PROCEDURE for DIRECT BILIRUBIN
Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path
Read against distilled water.
CALIBRATION TEST
R1 BILIRUBIN DIRECT 4+1 800 L 800 L
Calibrator 100 L
Sample 100 L
Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD1 OD2
Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 L 200 L
Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD3 OD4
CALCULATION FOR BOTH PROCEDURES (Total and Direct Bilirubin)
With Factor: (OD4 - OD2) x F
mg/dL: F = 14.0
mg/L: F = 140
mol/L: F = 240
With calibrator: OD4 - OD2 x n
OD3 - OD1
n = Calibrator concentration
NOTE: For more details, see the Instructions for use (08/2009)
MM-BITD-2
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CALCIUM ARSENAZO
Ref.: CALA - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 650 nm (600) Temperature : 37C Cuvette : 1 cm light path Read against distilled water.
Mix and read the optical density (OD) after a 1 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2005) MM-CALA-1
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CALCIUM OCPC
Ref.: CALO - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 570 nm Temperature : 37C Cuvette : 1 cm light path Read against distilled water.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2005) MM-CALO-1
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
(09/2009)
MM-CHLO-2
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHLORIDE
Rf. : CHLO 0600 2 x 125 mL 250 mL
MANUAL METHOD
PROCEDURE
Wavelength : 480 nm
Temperature : 25C, 30C, 37C
Cuvette : 1 cm light path
Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.
Information for urine sample: The urine sample must be diluted with distilled water before analysis.
CALCULATION
Serum/Plasma: Urine:
n StandardOD
SampleOD 2n
StandardOD
SampleOD
n= Standard concentration
NOTE: For more details, see the Instructions for use
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHOLESTEROL SL
Rf. : CHSL- 0490 1 x 100 mL CHSL- 0500 6 x 100 mL CHSL- 0700 4 x 250 mL CHSL- 0507 6 x 100 mL* CHSL- 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE Wavelength : 500 nm (492-550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration NOTE: For more details, see the Instructions for use
(07/2007) * these kits contain a standard MM-CHSL-2
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHOLESTEROL
Ref.: CHOL - 0220 12 x 20 mL CHOL - 0420 9 x 50 mL
MANUAL METHOD
PROCEDURE Wavelength : 500 nm (492 550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(03/2010) MM-CHOL-3
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHOLESTEROL HDL SL 2G
Ref.: HDLL 0380 1 x 80 mL HDLL 0390 3 x 80 mL
MANUAL METHOD
PROCEDURE Wavelength : 600 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. BLANK STANDARD SAMPLE R1 900 L 900 L 900 L Distilled water 9 L Standard 9 L Sample 9 L Mix and read absorbance (A1) after 5 minutes of incubation at 37C. Then add: R2 300 L 300 L 300 L Mix and read absorbance (A2) after 5 minutes of incubation at 37C CALCULATION
nCalibratorFdilAA
SampleFdilAA
)()(
12
12 n = Calibrator concentration
Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume) NOTE: For more details, see the Instructions for use
(04/2009) MM-HDLL-1
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHOLESTEROL LDL SL 2G
Ref.: LDLL 0380 1 x 80 mL
MANUAL METHOD
PROCEDURE Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. BLANK STANDARD SAMPLE R1 900 L 900 L 900 L Distilled water 9 L Standard 9 L Sample 9 L Mix and read absorbance (A1) after 5 minutes of incubation at 37C. Then add: R2 300 L 300 L 300 L Mix and read absorbance (A2) after 5 minutes of incubation at 37C CALCULATION
nCalibratorFdilAA
SampleFdilAA
)()(
12
12 n = Calibrator concentration
Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume) NOTE: For more details, see the Instructions for use
(04/2009) MM-LDLL-1
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
HDL
CHOLESTEROL
Ref.: HDLC - 0060 3 x 10 mL x 100 mL
MANUAL METHOD
PROCEDURE
1) Sample preparation: Add to 500 L of sample, 50 L of precipitating reagent. Mix, wait for 10 minutes and centrifuge at 5000 rpm
for 15 minutes. The supernatant is collected for HDL determination.
2) HDL determination:
Wavelength : 500 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path
Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.
CALCULATION
OD Sample x n x 1.1
OD Standard
n = Standard concentration
1.1 = Sample dilution factor
NOTE: For more details, see the Instructions for use
(09/2009)
MM-HDLC-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 50 L
Standard 50 mg/dL 50 L
Supernatant 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CHOLINESTERASE
Ref.: CHES - 0053 16 x 3 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm (400 440) Temperature : 25C, 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and measure the change of optical density every 30 seconds (OD/30 s.) during 90 seconds. CALCULATION Activity (U/L) = OD/30 s. x 23 460 LINEARITY If OD/30 sec. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/5 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(03/2008) MM-CHES-2
Working reagent 1 : 3 mL Sample : 20 L Working reagent 2 : 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CK MB SL
Rf. : CMSL- 0410 2 x 62.5 mL CMSL- 0430 4 x 62.5 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 3 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. ) Two-reagent procedure
Mix and wait 30 seconds
Mix and after a 5 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 8254 LINEARITY Inhibition : up to 2 000 U/L of CK-MM at 37 C. If OD/min. exceeds 0.075 dilute the sample (one part with nine parts saline solution), repeat the test and multiply the result by 10. Linear up at least 600 U/L. NOTE: For more details, see the Instructions for use
(11/2008) ): Modification from the previous version MM-CMSL-2
Working reagent : 1 mL Sample : 40 L
R1 : 1 mL R2 : 250 L
Sample : 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CK MB
Rf. : CKMB- 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 5 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION
a) Total CK activity : Determine total CK activity in serum using the CK-NAC reagent. b) CK-B activity : CK-B (U/L) = OD/min x 3 333 c) CK-MB activity : CK-MB (U/L) = CK-B (U/L) x 2 d) Percentage of CK-MB activity in sample : % CK-MB = CK-MB x 100 Total-CK
LINEARITY Inhibition : up to 2000 U/L of CK-MM at 37 C. If the assay of the total CK exceeds 1200 U/L at 37C, dilute the sample appropriately with physiological saline before assay of CK-MB. Multiply the result by the dilution factor to obtain the correct value of the isoenzyme. NOTE: For more details, see the Instructions for use
(05/2006) MM-CKMB-2
Working reagent : 1 mL Sample : 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CK NAC SL
Rf. : CKSL- 0410 2 x 62.5 mL CKSL- 0430 4 x 62.5 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. ) Two-reagent procedure
Mix and wait 30 seconds
Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 4127 LINEARITY If OD/min. exceeds 0.250 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(11/2008) ): Modification from the previous version MM-CKSL-2
Working reagent : 1 mL Sample : 40 L
R1 : 1 mL R2 : 250 L
Sample : 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CK NAC
Rf. : CKNA- 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm (334-365) Temperature : 25C, 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 340 nm : Activity (U/L) = OD/min. x 8 095 At 334 nm : Activity (U/L) = OD/min. x 8 252 At 365 nm : Activity (U/L) = OD/min. x 14 571
LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(03/2008) MM-CKNA-2
Working reagent : 1 mL Sample : 20 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
COPPER
Rf. : CUIV- 0050 5 x 10 mL
MANUAL METHOD
PROCEDURE Wavelength : 580 nm (570-590) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. One-reagent procedure
Mix and read the optical density (OD) after a 5 minute incubation. Two-reagent procedure
Mix and read the optical density (OD1).
Mix and read the optical density (OD2) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION One-reagent procedure : OD Sample x n OD Standard
Two-reagent procedure : OD2 - OD1 Sample x n OD2 - OD1 Standard
n = standard concentration NOTE: For more details, see the Instructions for use
(07/2005) MM-CUIV-1
BLANK STANDARD SAMPLE Working Reagent 2 1 mL 1 mL 1 mL Distilled Water 50 L Standard 50 L Sample 50 L
BLANK STANDARD SAMPLE Working Reagent 1 1 mL 1 mL 1 mL Distilled Water 50 L Standard 50 L Sample 50 L
Reagent 2 50 L 50 L 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CREATININE JAFFE
Ref.: CRCO - 0600 2 x 125 mL CRCO - 0700 4 x 250 mL
MANUAL METHOD
PROCEDURE Wavelength : 492 nm (480 520) Temperature : 37C Cuvette : 1 cm light path Read against distilled water.
Mix and read the optical density (OD1) 10 seconds after the sample or standard addition. Exactly 2 minutes after the first reading, take second reading (OD2). CALCULATION OD Sample x n OD Standard Take dilution factor into account for calculation of concentration in urine. NOTE: For more details, see the Instructions for use
(07/2005) MM-CRCO-1
Working reagent : 1 mL Sample : 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
CREATININE PAP SL
Ref.: CRSL - 0630 2 x 133 mL
MANUAL METHOD
PROCEDURE Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix, and wait 5 minutes at 37C. Then, add:
Mix and read the change of optical density (OD) between 1 and 4 minutes.. CALCULATION OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.
NOTE: For more details, see the Instructions for use
(03/2008) MM-CRSL-3
BLANK STANDARD SAMPLE R1 1.5 mL 1.5 mL 1.5 mL Distilled Water 35 L Standard 35 L Sample 35 L
R2 0.5 mL 0.5 mL 0.5 mL
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
GAMMA GT SL
Ref.: GASL - 0400 2 x 62.5 mL GASL - 0420 4 x 62.5 mL GASL - 0500 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION
One reagent procedure Two reagents procedure At 405 nm: Activity (U/L) = OD/min. x 1 158 1421 LINEARITY If OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-GASL-1
Working reagent : 1 mL Sample : 100 L
R1 : 1 mL Sample : 100 L
R2 : 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
GAMMA GT
Ref.: GAGT 0030 20 x 3 mL GAGT 0200 12 x 20 mL
MANUAL METHOD
PROCEDURE Wavelength : 405 nm Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 405 nm: Activity (U/L) = OD/min. x 1 111 LINEARITY If OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use
(07/2005) MM-GAGT-1
Working reagent : 1 mL Sample : 100 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
GLUCOSE HK SL
Ref.: GHSL - 0600 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm (334-365) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. One-reagent procedure
Mix and read the optical density (OD) after a 5 minute incubation. Two-reagents procedure
Mix, wait 1 minute and add:
Mix and read the optical density (OD) after a 5 minute incubation. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(07/2005) MM-GHSL-1
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
BLANK STANDARD SAMPLE R1 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
R2 250 L 250 L 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE
GLUCOSE PAP SL
Ref.: GPSL - 0490 1 x 100 mL GPSL - 0500 6 x 100 mL GPSL - 0700 4 x 250 mL GPSL - 0507 6 x 100 mL* GPSL - 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE Wavelength : 500 nm (492-550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and measure the optical density (OD) after a 10 minute incubation. The reading can be done during 30 minutes. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(07/2007) * these kits contain a standard MM-GPSL-2
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
GLUCOSE PAP
Ref.: GLUP - 0700 4 x 250 mL GLUP - 0800 5 x 500 mL
MANUAL METHOD
PROCEDURE Wavelength : 500 nm (492-550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 10 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(07/2005) MM-GLUP-1
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
IRON CHROMAZUROL
Ref.: FECA - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 637 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 15 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(07/2005) MM-FECA-1
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 50 L Standard 50 L Sample 50 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
IRON FERROZINE
Ref.: FEFR 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 560 nm (550-570) Temperature : 37C Cuvette : 1 cm light path Read against distilled water.
Mix and read the optical density (OD).
Then add:
Mix and read the optical density (OD) within one hour.
CALCULATION OD4 - OD2 x n OD3 - OD1 n = standard concentration
NOTE: For more details, see the Instructions for use
(09/2009) MM-FEFR-2
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 200 L Standard 200 L Sample 200 L
- OD1 OD2
R2 50 L 50 L 50 L
- OD3 OD4
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
LACTATE
Rf. : LACT- 0100 10 x 10 mL
MANUAL METHOD
PROCEDURE Wavelength : 540 nm (500-550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. CALCULATION OD Sample x n OD Standard
n= Standard concentration NOTE: For more details, see the Instructions for use
(05/2006) MM-LACT-2
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
LDH-L SL
Ref.: LLSL - 0400 2 x 62.5 mL LLSL - 0420 4 x 62.5 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. Two-reagent procedure BLANK SAMPLE R1 1 mL 1 mL Distilled Water 35 L Sample 35 L Mix, wait 3 minute and add: R2 250 L 250 L Mix and after a 100 second incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = OD/min. x 5828 NOTE: For more details, see the Instructions for use
(10/2007) MM-LLSL-1
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
LDH-P
Ref. : LDHP - 0030 20 x 3 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm (334-365) Temperature : 30C, 37C Cuvette : 1 cm light path Read against distilled water.
Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = OD/min. x 8095 At 334 nm: Activity (U/L) = OD/min. x 8252 At 365 nm: Activity (U/L) = OD/min. x 15000 LINEARITY If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/5 or 1/10 with sodium chloride solution (9 g/L). Multiply result by 5 or 10. NOTE: For more details, see the Instructions for use
(03/2008) MM-LDHP-2
Working reagent : 1 mL Sample : 20 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
MAGNESIUM CALMAGITE
Ref.: MAGN - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 520 nm (500-550) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration Take dilution factor into account for the calculation of magnesium concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2005) MM-MAGN-1
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
MICROPROTEIN
Ref.: PRTP - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 598 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. ) The final colour is stable for 10 minutes. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(11/2008) ): Modification from the previous version MM-PRTP-2
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 20 L Standard 20 L Sample 20 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
PHOSPHORUS
Ref.: PHOS - 0600 2 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard
n= Standard concentration Take dilution factor into account for the calculation of phosphorus concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2005) MM-PHOS-1
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
(08/2009)
MM-PROB-1
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
TOTAL PROTEIN PLUS
Rf.: PROB - 0600 2 x 125 mL
PROB - 0700 4 x 250 mL
MANUAL METHOD
PROCEDURE
Wavelength : 546 nm
Temperature : 37C
Cuvette : 1 cm light path
Read against reagent blank.
Mix and read the optical density (OD) after 10 minutes incubation.
The final colour is stable for 20 minutes.
CALCULATION
nStandardOD
SampleOD
n= Standard concentration
NOTE: For more details, see the Instructions for use
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE
TRIGLYCERIDES MONO SL NEW
Rf. : TGML-0425 6 x 50 mL TGML-0515 6 x 100 mL TGML-0700 4 x 250 mL TGML-0427 6 x 50 mL* TGML-0517 6 x 100 mL* TGML-0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE Wavelength : 500 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 10 minute incubation. CALCULATION OD Sample x n OD Standard
n= Standard concentration NOTE: For more details, see the Instructions for use
(07/2007) * these kits contain a standard MM-TGMLN-2
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
TRIGLYCERIDES
Ref.: TRIG - 0200 12 x 20 mL TRIG - 0400 9 x 50 mL
MANUAL METHOD
PROCEDURE Wavelength : 546 nm (520 570) Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 30 minutes. CALCULATION OD Sample x n OD Standard
n= Standard concentration
NOTE: For more details, see the Instructions for use
(07/2007) MM-TRIG-2
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
UREA UV SL
Rf. : URSL- 0400 2 x 62.5 mL URSL- 0420 4 x 62.5 mL URSL- 0500 5 x 125 mL URSL- 0407 2 x 62.5 mL* URSL- 0427 4 x 62.5 mL* URSL- 0507 5 x 125 mL*
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure
Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds. CALCULATION OD Sample x n OD Standard n = standard concentration NOTE: For more details, see the Instructions for use
(07/2007) * these kits contain a standard MM-URSL-2
Working reagent : 1 mL Sample : 10 L
R1 : 1 mL Sample : 10 L
R2 : 250 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
UREA UV
Rf. : URUV-0400 9 x 50 mL URUV-0500 6 x 100 mL
MANUAL METHOD
PROCEDURE Wavelength : 340 nm Temperature : 37C, 30C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds. CALCULATION OD Sample x n OD Standard
n= Standard concentration
Take the dilution factor into account for the calculation of urea concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2005) MM-URUV-1
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 L Standard 10 L Sample 10 L
ELITECH ELITECH ELITECH
SEPPIM S.A..S- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
URIC ACID
Rf. : ACUR - 0200 12 x 20 mL
ACUR - 0400 9 x 50 mL
MANUAL METHOD
PROCEDURE
Wavelength : 510 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path
Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 15 minutes.
CALCULATION
OD Sample x n
OD Standard
n= Standard concentration
Take the dilution factor into account for the calculation of uric acid concentration in urine.
NOTE: For more details, see the Instructions for use
(09/2009)
MM-ACUR-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 25 L
Standard 25 L
Sample 25 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
URIC ACID MONO SL
Rf. : AUML - 0420 6 x 50 mL AUML - 0500 6 x 100 mL AUML - 0700 4 x 250 mL AUML - 0427 6 x 50 mL* AUML - 0507 6 x 100 mL* AUML - 0707 4 x 250 mL*
MANUAL METHOD
PROCEDURE Wavelength : 555 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank.
Mix and read the optical density (OD) after a 5 minute incubation. CALCULATION OD Sample x n OD Standard
n= Standard concentration
Take the dilution factor into account for the calculation of uric acid concentration in urine.
NOTE: For more details, see the Instructions for use
(07/2007) * these kits contain a standard MM-AUML-2
BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 25 L Standard 25 L Sample 25 L
ELITECH ELITECH ELITECH
SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
URIC ACID SL
Rf. : AUSL 0400 2 x 62.5 mL AUSL 0600 5 x 125 mL
MANUAL METHOD
PROCEDURE Wavelength : 555 nm Temperature : 37C Cuvette : 1 cm light path Read against reagent blank. One-reagent procedure
Mix and read the optical density (OD) after a 5 minute incubation. Two-reagent procedure
Mix, wait 1 minute and add:
Mix and read the optical density (OD) after a 5 minute incubation. CALCULATION OD Sample x n OD Standard
n= Standard concentration Take the dilution factor into account for the calculation of uric acid concentration in urine.
NOTE: For more details, see the Instructions for use
(03/2010) MM-AUSL-2
BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 25 L Standard 25 L Sample 25 L
BLANK STANDARD SAMPLE R1 1 mL 1 mL 1 mL Distilled Water 25 L Standard 25 L Sample 25 L
R2 250 L 250 L 250 L
MAGNESIUM CALMAGITE ................... MM-MAGN-1