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IAG ring test animal proteins 2013

L. van Raamsdonk, V. Pinckaers, J. Vliege

Goals

● Quality support of participants

● Method performance

Ring test animal proteins


Procedure

● Preparation of samples; send 5 March

● Examination; return 12 April

● Preparation of report; draft ready 6 June


Materials, based on an artificial feed

● Blank sample

● Sample with fish meal 2%

● Sample with 0.05% ruminant MBM (Uruguay)

● Sample with 0.1% of tricalcium phosphate (TCP)


Microscopic method

● New amended version of R (EC) 152/2009

● Effective from 12 February, but no official SOPs at the time of the ring test

● New elements in procedure

● Implemented in ring test: examination of flotate or raw material

● Not implemented: repetition of examination

● Not implemented: official report texts


microscopy 53 participants; 53 sets

PCR 7 participants; 10 targets

Immunochemistry none

Response


Results

● Background of materials

● Accuracy fish

● Accuracy terrestrial animals

● Accuracy flotate or raw material

● Quantification

● Application of method parameters

● Results of PCR

● Evaluation


0.96

0.98

0.92

A: blank

B: 2 % fish

C: 0.05 % MBM

D: 0.1 % TCP

specificity sensitivity

0.98

Results fish


0.94

0.98


1.0

0.92 *

Results terrestrial animals

A: blank

B: 2 % fish

C: 0.05 % MBM

D: 0.1 % TCP

*: EU member states only: 0.98


TCP identification


0.91


0.83

0.34

0.68

Results flotate or raw sample material (target: animal material in general)

A: blank

B: 2 % fish

C: 0.05 % MBM

D: 0.1 % TCP


Method application

● Tendency to make smaller slides (small cover glass: n=38)

● Increasing use of special glass with cock

● Decreasing use of staining of sediment

● Unchanged frequency of using a binocular

● Amount of sediment used: 1 – 100 %


10 gram of material

200 milligram sediment

20 mg

Examination:

20 mg

1

gr

Example: 0.05% 20 particles

20 p

Result: positive

2 p

Result: suspect

Amount of sediment used


1.0

1.0

0.81

< 50% >= 50%

n=14 n=24

1.0

1.0

1.0

A: blank

B: 2 % fish

C: 0.05 % MBM

D: 0.1 % TCP *

*: p = 0.053

Amount of sediment used: sensitivity


Recommendations

● Improvement of examination of flotate or raw

material

● Method: full application

● Harmonisation of the method

● Further attention to detection of TCP


Thanks to:

● All participants of the study

● IAG board for cooperation

Thank you for

your attention

Questions?