Investigation of the BARREN STALK1 gene lineage in graminoid Poales
Daniel Woods
rice corn
Outline
1) Introduction into field of Evolutionary Developmental Genetics and Grasses
2)What’s BARREN STALK1
3) Hypothesis/aims
4) Preliminary Data
rice
corn
Graminoid Poales
?
phylogenetics
morphologicaldevelopment
molecular evolution
functional analyses
gene expression
Chasmanthium (CEN) inflorescence development
barren stalk1 (ba1) phenotypeWild-type tassel ba1-ref WT meristem ba1-mum1
Gallavotti, A., et al. (2004). Nature 432(7017): 630-5
Gallavotti, A., et al. (2004). Nature 432(7017): 630-5
Rice (OsLAX)Maize (ZmBA1)
BA1/LAX expression via in situ hybridization
Gallavotti, A., et al. (2004), Nature 432(7017): 630-5 Komatsu, K., et al. (2003). Proc Natl Acad Sci USA 100(20): 11765-70
Hypotheses:
1) BA1 duplication event occurred at base of grass family
2) BA1 is expressed in branching primordia throughout inflorescence development
Specific aims to test hypothesis
1) Isolate BA1 bHLH genes from diverse array of grasses and near grass relatives
2) Generate phylogenies to pinpoint duplication event
3) Document inflorescence development using Scanning Electron Microscopy (SEM)
4) Gene Expression Analysis via in situ hybridization
BEP PACCMAD
Gene Isolation and Preparation
Extractions Primer Design
Polymerase Chain Reaction(Semi-nested)
Gel Extraction
Ligation
Clone
PCR screen
Alkaline lysis
Sequence(Sanger)
Generate Phylogenies
Maximum Parsimony
Maximum Likelihood
Bayesian