MolecularbiologyMolecularbiology
Green Fluorescent Protein
Alba, the fluorescent bunnyAlba, the fluorescent bunny
Alba, the fluorescent bunnyAlba, the fluorescent bunny
• "Alba", the green fluorescent bunny, is an albino rabbit.
• Alba is not green all the time.• It only glows when illuminated with the correct
light.• When (and only when) illuminated with blue light
(maximum excitation at 488 nm), she glows with a bright green light (maximum emission at 509 nm).
GFP - Other ApplicationsGFP - Other Applications
GFP Transgenic MiceGFP Transgenic Mice
Toes of newborn mice
Pancreatic tissue
Aequorea victoria (jellyfish)Aequorea victoria (jellyfish)
Green Fluorescent ProteinGreen Fluorescent Protein
E. coliE. coli
cells carrying and expressingthe gfp gene of A. victoria
wild type colonies
How to do it?How to do it?
?
Applications of CloningApplications of Cloning
PharmacyPharmacy
MedicineMedicine
FoodFood
AgricultureAgriculture
EnvironmentalEnvironmentalcleanupcleanup Basic researchBasic research
RegulationsRegulationsSafety & HealthSafety & Health
CloningCloning
Ethical concernsEthical concerns
Acceptance of genetic engineeringAcceptance of genetic engineering
24
74
76
26
crops &food
pharmacy &medicine
data given in % Pro Contra
24
74
76
26
crops &food
pharmacy &medicine
data given in % Pro Contra
Source: Akademie für Technikfolgenabschätzung / Baden-Württemberg, 1998