Phage Nanobiotechnology Tatiana Samoylova Phages ideal
naturally occurring nanomaterials phages - viruses that infect
bacteria exhibit a great diversity of shapes in nanometer range
(examples below) well-defined geometry and uniformity favorable for
nanofabrication phage coat proteins can be engineered using simple
recombinant DNA techniques Phage Lambda,
http://textbookofbacteriology.net/phage_2. html Filamentous M13
phage, EM image. T. Samoylova Segment of ~1% of filamentous
landscape phage particle. Phage coat proteins are grey. Engineered
fusion peptides are black. V. Petrenko.
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Phage Nanobiotechnology Tatiana Samoylova 7 nm 900 nm Phage
display technology set of phage-based techniques for identification
of target-specific peptides or proteins targets include
biomolecules, whole cells, whole tissues in living organisms,
non-biological surfaces (for example, metals) phage display
libraries are mixtures of distinct phage clones filamentous phage
is predominant phage display vector library consists of billions of
phage clones billions opportunities to select phage clones specific
to desired targets pIII pVI pVIII pVII + pIX
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Phage Nanobiotechnology Tatiana Samoylova pIII pVI pVIII pVII +
pIX 7 nm g8 900 nm Phage display technology set of phage-based
techniques for identification of target-specific peptides or
proteins targets include biomolecules, whole cells, whole tissues
in living organisms, non-biological surfaces (for example, metals)
phage display libraries are mixtures of distinct phage clones
filamentous phage is predominant phage display vector library
consists of billions of phage clones billions opportunities to
select phage clones specific to desired targets
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Phage Nanobiotechnology Tatiana Samoylova What we are doing
with phage nanomaterials (current focus) whole phage particles as
vaccines for contraception of animals (cat/dog/pig) phage particles
that display peptides with contraceptive properties -GnRH peptide,
zona-pellucida binding peptides -phage body carrier protein,
peptides antigens phage is effective immunogen (nanosize,
architecture), low cost, stable, safe shown below sperm-oocyte
interaction in in vitro fertilization experiment in the presence of
(A) normal serum or (B) serum from immunized animal Normal porcine
serum Porcine anti-serum, contains anti- sperm antibodies A B
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Phage Nanobiotechnology Tatiana Samoylova What others at AU are
doing with phage nanomaterials targeted liposomes with phage fusion
proteins for cancer-specific drug/gene delivery (V Petrenko, CVM,
illustration below) devices for pathogen detection using
filamentous phage (Bryan Chin, AU Detection and Food Safety Center)
devices for detection of bacterial infection using lytic phage
(Aleksandr Simonian, College of Engineering) fabrication of
phage-based biosensors (V Vodyanoy, CVM) magnetic nanoparticles
targeted with phage-derived peptides for imaging of brain tumors (A
David, College of Engineering, illustration on the right) +
Landscape phage coat protein units displaying tumor- specific
peptide Therapeutic liposome containing drugs Tumor-targeted
liposome Multi-domain cores 100 nm Magnetic nanoparticles targeted
to brain tumor with phage-derived peptides
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Phage Nanobiotechnology Tatiana Samoylova Phage nanomaterials
in medicine and technology: applications affinity matrix for
purification of biomolecules scaffold for tissue engineering/repair
medical diagnostics and monitoring molecular imaging targeted drug
and gene delivery vaccine development detection of pathogens and
biothreats in microelectronics, as template for fabrication of
nanowires and nanocomposite films details in this book New hires
for AU bioinformatics computational proteomics systems biology
(systeomics)