Edition 27, 08/05/2017 101T Page 1 of 17
Public Health Laboratory Health Service Executive (Dublin Mid Leinster)
Cherry Orchard Hospital, Ballyfermot, Dublin 10
Testing Laboratory
Registration number: 101T
is accredited by the Irish National Accreditation Board (INAB) to undertake
testing as detailed in the Schedule bearing the Registration Number detailed
above, in compliance with the International Standard ISO/IEC 17025:2005 2nd Edition
“General Requirements for the Competence of Testing and Calibration Laboratories”
(This Certificate must be read in conjunction with the Annexed
Schedule of Accreditation)
Date of award of accreditation: 02:05:2002
Date of last renewal of accreditation: 08:05:2017
Expiry date of this certificate of accreditation: 08:05:2022
This Accreditation shall remain in force until further notice subject to continuing compliance
with INAB accreditation criteria, ISO/IEC 17025 and any further requirements specified by the
Irish National Accreditation Board.
Manager: Chairperson: Dr Adrienne Duff Ms. Ita Kinahan
Issued on 08 May 2017
Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this
Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please
contact the Irish National Accreditation Board.
The INAB is a signatory of the European co-operation for Accreditation (EA) Testing Multilateral Agreement (MLA) and the
International Laboratory Accreditation Cooperation (ILAC) Mutual Recognition Arrangement.
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Schedule of Accreditation
(Annex to Accreditation Certificate) Permanent Laboratory:
Category A
PUBLIC HEALTH LABORATORY
Health Service Executive, Dublin Mid Leinster
Biological Testing Laboratory
Initial Registration Date : 30-November-1998
Postal Address:
(Address of other locations
as they apply)
Public Health Laboratory
Health Service Executive
Dublin Mid Leinster
Cherry Orchard Hospital
Ballyfermot
Dublin 10
Telephone: +353 (1) 07669 55175
Fax: +353 (1) 6231908
E-mail: [email protected]
Contact Name: Dr Eleanor Mc Namara
Facilities: Public testing service
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Schedule of Accreditation
Permanent Laboratory: Category A
THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of
organisations including laboratories.
Laboratory accreditation is available to testing and calibration facilities operated by manufacturing
organisations, government departments, educational institutions and commercial testing/calibration
services. Indeed, any organisation involved in testing, measurement or calibration in any area of
technology can seek accreditation for the work it is undertaking.
Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria
which are in compliance with ISO/IEC 17025 or ISO 15189 (medical laboratories). Frequent audits,
together with periodic inter-laboratory test programmes, ensure that these standards of operation are
maintained.
Testing and Calibration Categories:
Category A:
Permanent laboratory calibration and testing where the laboratory is erected on a fixed
location for a period expected to be greater than three years.
Category B: Site calibration and testing that is performed by staff sent out on site by a permanent
laboratory that is accredited by the Irish National Accreditation Board.
Category C: Site calibration and testing that is performed in a site/mobile laboratory or by staff sent
out by such a laboratory, the operation of which is the responsibility of a permanent
laboratory accredited by the Irish National Accreditation Board.
Category D: Site calibration and testing that is performed on site by individuals and organisations that
do not have a permanent calibration/testing laboratory. Testing may be performed using
(a) portable test equipment
(b) a site laboratory
(c) a mobile laboratory or
(d) equipment from a mobile or site laboratory
Standard Specification or Test Procedure Used:
The standard specification or test procedure that is accredited is the issue that is current on the date of the most
recent visit, unless otherwise stated.
Glossary of Terms
Facilities:
Public calibration/testing service: Commercial operations which actively seek work from others.
Conditionally available for public
calibration/testing:
Established for another primary purpose but, more commonly than not,
is available for outside work.
Normally not available for public
calibration/testing:
Unavailable for public calibration/testing more often than not.
Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish
National Accreditation Board criteria should insist on receiving an accredited calibration certificate or test report.
Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on
request, verify the status and scope.
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Scope of Accreditation
Public Health Laboratory HSE-Dublin Mid Leinster Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number
(P9)
Materials/products tested
Type of test/properties measured
Range of measurement
Standard specifications
Equipment/techniques used
811
.01
.02
.03
.04
.05
.06
.07
.08
.09
.10
.13
.14
.15
.16
.17
.18
.22
Microbiological
tests on foods
Dairy Products
Egg and egg products
Meat & meat products,
game and poultry
Fish, shellfish and
molluscs
Fats and oils
Soups, broths & sauces
Cereals and bakery
products
Fruit and vegetables
Herbs and spices
Non-alcoholic
beverages
Ices and desserts
Cocoa and cocoa
preparations, coffee
and tea
Confectionary
Nuts and nut products,
snacks
Prepared dishes
Foodstuffs intended
for special nutritional
uses
Pet Foods
Total viable counts at 30°C
(Spiral plate)
Enumeration and confirmation of Escherichia
coli by TBX agar
(Spread plate / chromogenic agar TBX)
Enumeration and confirmation of coagulase
positive staphylococci (Staphylococcus aureus
& other species)
(Spread plate)
Enumeration and confirmation of Bacillus
species including presumptive Bacillus cereus
(Spiral plate)
Enumeration & confirmation of Clostridium
perfringens
(Spread plate / Pour plate)
Detection & confirmation of Salmonella
species
(Enrichment/selective media)
Documented in house method:
SFM 001 based on
ISO 13559:2002
ISO 4833:2013
SFM 002 based on
ISO 16649-2:2001
SFM 003 based on
ISO 6888-1:1999/Amd 1:2003
SFM 004 based on
ISO 7932:2005
SFM 005 based on
ISO 7937:2004
*The Microbiology of Drinking
Water (2010) Part 6.
*Refers to confirmation stage
only
SFM 006 (Culture) based on:-
ISO 6579:2002/Amd 1:2007
ISO 6785:2007(dairy only)
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Scope of Accreditation
Public Health Laboratory HSE-Dublin Mid Leinster Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties measured
Range of measurement
Standard specifications
Equipment/techniques used
811
.01
.02
.03
.04
.05
.06
.07
.08
.09
.10
.13
.14
.15
.16
.17
.18
.22
Microbiological tests
on foods
Dairy Products
Egg and egg products
Meat & meat products,
game and poultry
Fish, shellfish &
molluscs
Fats and oils
Soups, broths & sauces
Cereals and bakery
products
Fruit and vegetables
Herbs and spices
Non-alcoholic
beverages
Ices and desserts
Cocoa and cocoa
preparations, coffee
and tea
Confectionary
Nut and nut products,
snacks
Prepared dishes
Foodstuffs intended
for special nutritional
uses
Pet Foods
Enumeration, detection and
confirmation of Listeria species
including Listeria monocytogenes
(Spread plate/enrichment)
Detection and confirmation of
Campylobacter species
(Enrichment)
Enumeration and confirmation of
Enterobacteriaceae
(Pour plate)
Detection and confirmation of
E.coli O157
(Enrichment/IMS)
Detection and confirmation of
Salmonella species
(Enrichment /Vidas)
Documented in house method:-
SFM 007 based on
ISO 11290-1:1997/Amd 1:2004
ISO 11290-2:1999/Amd 1:2004
SFM 008 based on
ISO 10272-1:2006
(Culture)
SFM 009 based on
ISO 21528-1:2004
ISO 21528-2:2004
SFM 011 based on
ISO 16654:2001
SFM 015 (Vidas) based on:-
Association Francaise de Normalisation
AFNOR (Bio-12/1-04/94):2004
ISO 6579:2002/Amd 1:2007
ISO 6785:2007 (dairy only)
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
811
.03
.08
.09
Microbiological tests
on foods
Meat and meat
products, game and
poultry
Fruit and vegetables
Herbs and spices
Detection and confirmation of
E. coli O26
Documented in house method:
SFM 022 based on
ISO 16654:2001
(Enrichment/IMS)
.10
Non-alcoholic
beverages
- Bottled waters
Enumeration and confirmation
of Coliforms [cfu/250ml]
Enumeration & confirmation of
Escherichia coli [cfu/250ml]
Enumeration of coliform
bacteria and E.coli by
membrane filtration
SWM 001 based on
ISO 9308-1: 2014
SWM 001 based on ISO 9308-
1:2014 - Enumeration of
Escherichia coli and
coliforms
ISO 8199: 2005 - General
guidance on the enumeration
of microorganism by culture
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
811
.10
Microbiological tests
on foods
Non-alcoholic
beverages
- Bottled waters
Enumeration & confirmation of
Enterococci [cfu/250ml]
Enumeration and confirmation
of Clostridium perfringens
[cfu/50ml]
Enumeration and confirmation
of Pseudomonas aeruginosa
[cfu/250ml]
Colony count at 22°C and 37°C
[cfu/ml] (Excluding bathing
water)
Documented in house method:
SWM 003 based on
ISO 7899-2:2000
(membrane filtration)
SWM 004 based on
The Microbiology of Drinking
Water (2010) Part 6.
(membrane filtration)
SWM 008 based on
The Microbiology of Drinking
Water (2010) Part 8
(membrane filtration)
SWM 006 and SWM 007 based
on
ISO 6222:1999 (Pour
plate)
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
818
.01
Microbiological tests
for factory hygiene
purposes
Surfaces
Detection and enumeration of
Aerobic colony count (surface
swabs)
Detection and enumeration of
E.coli (surface swabs)
SFM 001 based on
ISO 18593:2004
ISO 4833:2003
(Spiral plate)
SFM 002 based on
ISO 16649-2:2001
ISO 18593:2004
(Spiral plate / Pour Plate)
819
.99
Microbiological tests
on other materials
Other Materials
Environmental swabs
including those from
healthcare facilities
Detection of Pseudomonas
aeruginosa (environmental
swabs)
SEM.001 based on UK Dept.
of Health, Health Technical
Memorandum 04-01
Addendum: Pseudomonas
aeruginosa - advice for
augmented care units 2013.
ISO 18593: 2004 Microbiology
of food and animal feeding
stuffs- Horizontal methods
for sampling techniques from
surfaces using contact plates
and swabs.
I.S. EN ISO 16266:2008 Water
Quality - Detection and
enumeration of Pseudomonas
aeruginosa- Method by
membrane
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Scope of Accreditation
Public Health Laboratory HSE-Dublin Mid Leinster Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification
number (P9)
Materials/products tested
Type of test/properties measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.11
.15
.16
.99
Waters
Bacteriological
condition of
potable water
Bacteriological
condition of
swimming pools
and spas
Bacteriological
condition of
environmental
waters
Other tests
Source water
Enumeration and confirmation of Coliforms
[cfu/100ml]
Enumeration & confirmation of Escherichia
coli [cfu/100ml]
Enumeration & confirmation of Enterococci
[cfu/100ml]
Enumeration and confirmation of
Clostridium perfringens
[cfu/100ml or cfu/50ml]
Enumeration and confirmation of
Pseudomonas aeruginosa
[cfu/100ml]
Documented in house
method: based on
The Microbiology of Drinking
Water. Methods for the
examination of waters and
associated materials.
SWM 001 based on
ISO 9308 1:2014
SWM 003 based on
ISO 7899-2:2000
(membrane filtration)
SWM 004 based on
The Microbiology of Drinking
Water (2010) Part 6.
(membrane filtration)
SWM 008 based on
The Microbiology of Drinking
Water (2010) Part 8
(membrane filtration)
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.11
.15
.16
.99
Waters
Bacteriological
condition of potable
water
Bacteriological
condition of swimming
pools and spas
Bacteriological
condition of
environmental waters
Other tests
Source water
Bathing water
Enumeration of Coliforms and
Escherichia coli
[cfu/100ml]
Colony count at 22°C and 37°C
[cfu/ml]
(Excluding bathing water)
Documented in house
methods
SWM 009 based on ISO 9308-
2: 2012 Water Quality -
Enumeration of E.coli and
Coliform Bacteria. Part 2:
Most probable number
method. The Microbiology of
Drinking Water (2009) Part 4
(IDEXX (Colilert 18)
QuantitrayTM)
( Most probable number)
SWM 006 and SWM 007
based on
ISO 6222:1999 (Pour plate)
870
.11
.16
Waters
Bacteriological
condition of potable
water
Bacteriological
condition of
environmental waters
Detection and confirmation of
E.coli O157
(Membrane
filtration/enrichment/IMS)
[Detected per Litre]
Detection and confirmation of
E.coli O26 (membrane
filtration/enrichment/IMS)
[Detected per Litre]
Documented in house
method
SWM 010 based on
The Microbiology of Drinking
Water (2009) Part 4.
SWM 020 based on The
Microbiology of Drinking
Water (2009) Part 4
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
881
DNA Analysis
Detection of Verotoxigenic E.
coli (VTEC) in water samples
using automated DNA
extraction and Real-Time PCR
Documented in house method SMM003
based on
1) ISO 13136: 2012
2) Technical specification for the
monitoring and reporting of VTEC on
animals and foods (VTEC surveys on
animal and food). Scientific report of
EFSA . EFSA Journal 2009:7 (11):1366
3) Identification of three vtx1 and
seven vtx2 subtypes of verotoxin
encoding genes of E. coli by
conventional PCR amplification. WHO
collaborating centre for reference and
research on Escherichia and Klebsiella.
Circulated by ECDC 2010.
The Microbiology of Drinking Water
(2002)-Part 3-Practices and procedures
for laboratories
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.11
.12
.15
.16
.99
Waters
Bacteriological condition
of potable waters
Bacteriological condition
of industrial
waters (treated,
recirculating)
Bacteriological condition
of swimming pools and
spas
Bacteriological condition
of environmental waters
Other tests
Source Water
Detection and confirmation
of Salmonella species
(Membrane
filtration/enrichment/vidas)
[Detected per Litre)
SWM 005 based on
ISO 19250:2010 (Culture)
SWM 011 (Vidas) based on
Association Francaise de
Normalisation AFNOR
(Bio-12/1-04/94):2004
ISO 19250:2010
.11
.99
Bacteriological condition
of potable waters
Other tests
- Well waters
- Hydrotherapy waters
Enumeration of coliform
bacteria and E.coli by
membrane filtration
SWM001 based on
ISO 9308-1:2014 -
Enumeration of Escherichia
coli and coliforms
ISO 8199: 2005 - General
guidance on the
enumeration of
microorganism by culture
.99 Other tests
- Waters for Dialysis
Total Viable Count / ml for
Dialysis by membrane
filtration
SWM023 based on ISO
23500:2014, ISO 13959:2014
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Scope of Accreditation
Public Health Laboratory HSE-Dublin Mid Leinster Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number
(P9)
Materials/products tested
Type of test/properties measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.16
.99
Waters
Bacteriological
condition of
environmental
waters
Other tests
Endoscopy
rinse waters
Total viable count at 30°C
[cfu/100ml]
Enumeration and confirmation of
Pseudomonas
aeruginosa [cfu/100ml]
Detection of slow-growing
Mycobacterium spp. From waters
from heater-cooler units
Documented in house methods
SWM 017 based on ISO 15883: Part 4
Requirements and tests for washer
disinfectors employing chemical
disinfection for thermolabile
endoscopes.
ISO 15883: Part 1
General requirements, terms and
definitions and tests.
SWM017 Total Viable Count/100 ml
for Environmental/Endoscop y
waters
SWM 008 based on
The Microbiology of Drinking Water
(2010)
Part 8 (membrane filtration )
SWM 018M base on ISO 15883-4
Requirements and tests for washer
disinfectors employing chemical
disinfection for thermolabile
endoscopes
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Scope of Accreditation
Public Health Laboratory HSE-Dublin Mid Leinster Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number
(P9)
Materials/products tested
Type of test/properties measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.99
Waters
Other tests
Bathing waters
Enumeration and confirmation of
Coliforms and E.coli by membrane
filtration [cfu/100ml]
Enumeration and confirmation of
Enterococci by membrane filteration
[cfu/100ml]
Detection and confirmation of
Salmonella species
[Membrane filtration-Detected per
Litre]
SWM 001 based on ISO 9308-1:2014
SWM 003 based on
ISO 7899-2:2000
SWM 005 based on
ISO 19250:2010 (Culture)
SWM 011 (Vidas) based on:-
Association Franchaise de Normalisation
AFNOR (Bio-12/1-04/94):2004
ISO 19250:2010
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
870
.11
.12
.15
.16
.99
Waters
Bacteriological
condition of potable
waters
Bacteriological
condition of industrial
waters (treated,
recirculating)
Bacteriological
condition of swimming
pools and spas
Bacteriological
condition of
environmental waters
Other tests
Endoscopy
rinse waters
Detection and Enumeration of
Legionella pneumophila
Serogroup 1
Serogroup 2 - 14
Legionella species
Documented in house
methods
SWM 012 based
ISO 11731: 1998
(membrane filtration)
.99 Other tests
Endoscopy rinse
waters
Detection of Mycobacterium
species in Endoscopy Waters
SWM 018 based on
Method for Analysis of
Endoscopy Waters
D. Stokely (2003)
(membrane filtration)
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Scope of Accreditation
Public Health Laboratory HSE-Dublin
Mid Leinster
Permanent Laboratory:
Category A
Biological Testing Laboratory
INAB Classification number (P9)
Materials/products tested
Type of test/properties
measured
Range of measurement
Standard specifications
Equipment/techniques used
881
DNA Analysis
Detection of verotoxigenic
E.coli (VTEC) by PCR (including
serogroups O26,
O103,O104,O111, O145, O157
and verotoxins vt1, vt2, vt2f)
SMM001 based on :-
Scientific report of EFSA.
EFSA journal
2009:7(11):1366
Clinical Laboratory
Standards Institute, MM17-
A: verification and
validation of multiplex
nucleic acid assays:
Approved guideline
881
DNA Analysis
Detection of verotoxigenic
E.coli (VTEC) by automated
DNA extraction and PCR
(including serogroups O26,
O103, O104,O111, O145,O157
and verotoxins vt1, vt2, vt2f)
SMM002 based on :-
1) ISO 13136: 2012
2) Scientific report of EFSA.
EFSA journal
2009:7(11):1366
3) Clinical Laboratory
Standards Institute, MM17-
A: verification and
validation of multiplex
nucleic acid assays:
Approved guideline