DNA Content and Relevant Markers

Breast CancerHoechst

CytokeratinTGF- Erb-B2

Analysis of Fine Needle Aspirates

RB121324hoechst ->0 256 512 768 1024

10 10 10 10 100 1 2 3 4

RB121324anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB121324anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB121324anti-TGF alpha ->

10 10 10 10 100 1 2 3 4

RB121324anti-cytokeratin ->

CELL SUSPENSION FROM ADJACENT NORMAL BREAST TISSUE

RB121324hoechst ->0 256 512 768 1024

CK+TGF+erb- CK-TGF+erb-

DNADNA RB121324hoechst ->0 256 512 768 1024

DNA

ALL CELLS

cytokeratin

cytokeratin

TG

Fa

lph

a

TGFalpha cytokeratin

erb

B2

erb

B2

hoech

st

10 10 10 10 100 1 2 3 4

RB102218anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB102218anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB102218anti-TGF alpha ->

10 10 10 10 100 1 2 3 4

RB102218anti-cytokeratin ->

CELL SUSPENSION FROM A BREAST TUMOR

ALL CELLSCK-TGF+erb-CK+TGF-erb-CK+TGF+erb-CK+TGF-erb+CK+TGF+erb+

RB102218hoechst ->0 256 512 768 1024

RB102218hoechst ->0 256 512 768 1024

RB102218hoechst ->0 256 512 768 1024

RB102218hoechst ->0 256 512 768 1024

RB102218hoechst ->0 256 512 768 1024

RB102218hoechst ->0 256 512 768 1024

DNA DNA DNA DNA DNA DNA

cytokeratin

cytokeratin

TG

Fa

lph

a

TGFalpha cytokeratin

erb

B2

erb

B2

hoech

st

RB08319421hoechst ->0 256 512 768 1024

RB08319421hoechst ->0 256 512 768 1024

RB08319421hoechst ->0 256 512 768 1024

10 10 10 10 100 1 2 3 4

RB08319421anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB08319421anti-cytokeratin ->

10 10 10 10 100 1 2 3 4

RB08319421anti-TGF alpha ->

10 10 10 10 100 1 2 3 4

RB08319421anti-cytokeratin ->

DNA DNARB08319421hoechst ->0 256 512 768 1024

DNA DNA

CELL SUSPENSION FROM A BREAST TUMOR

ALL CELLSCK-TGF+erb-CK+TGF-erb-CK+TGF+erb-

cytokeratin

cytokeratin

TG

Fa

lph

a

TGFalpha cytokeratin

erb

B2

erb

B2

hoech

st