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AZD8330Cat. No.: A8374
CAS No.: 869357-68-6
Formula: C16H17FIN3O4
M.Wt: 461.23
Synonyms: ARRY-424704; ARRY-704; AZD-8330;
ARRY424704; ARRY704; AZD8330
Target: MAPK Signaling
Pathway: MEK1/2
Storage: Store at -20°C
In Vitro
23.05mg/mL in DMSO
Preparing
Stock Solutions
Mass
1mg 5mg 10mgSolvent
Concentration
1 mM 2.1681 mL 10.8406 mL 21.6812 mL
5 mM 0.4336 mL 2.1681 mL 4.3362 mL
10 mM 0.2168 mL 1.0841 mL 2.1681 mL
Please refer to the solubility information to select the appropriate solvent.
Shortsummary MEK 1/2 inhibitor
IC₅₀ & Target 0.4 nM (ERK phosphorylation), 7 nM (MEK1/2)
In Vitro
Cell Viability Assay
Cell Line: MOS, U2OS, KPD, ZK58, 143b and Saos-2 cells
Preparation method The solubility of this compound in DMSO is >10 mM. General tips for obtaining
a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or
shake it in the ultrasonic bath for a while. Stock solution can be stored below -
20 °C for several months.
Reacting conditions: 0 ~ 1 μM; 72 hrs
Product Name: AZD8330Revision Date: 0 / /2020
Product Data Sheet
Solvent & Solubility
Biological Activity
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AZD8330Cat. No.: A837777777777744444444444
CAS No.: 8686668666686868686868868668688869393939399393939393935757575757575757575777----6866666666666 -6
Formula: CC1C1C1C1C1C1C1C1CCCCCC 6H17FIN3O4
M.Wt: 461.23
Synonyms: ARRY-YY 424704; ARRY-YY 704; AZZD-8330;
ARRY424704; ARRY704; AZDD8330
Target: MAPK Signaling
Pathway: MEK1/2
Storage: Store at -20°C
In Vitro
23.05mg/mL in DMSO
Preparing
Stock Solutions
Mass
1mg 5mg 10mgSolvent
Concentration
1 mM 2.1681 mL 10.8406 mL 21.6812 mL
5 mM 0.4336 mL 2.1666666666818181818181818118181 mmmmmmmmmmmLLLLLLLLLL 4.3362 mL
10 mM 0.2168 mL 1.1.1.1.1..1.1..1.08080808088880808088880808808888844444141414141414441414414144141 mL 2.1681 mL
PlPlPlPlPlPlPlPlPlPleaeeeaeaeaeaeeae seseseseseseseseseseese rrrrrrrrrefeeeee er to the solubility information to select the appropriate sssssssssssssolololololololololololololoo veveveveveeeveveeeevevevvevev ntntntntntntntntntntnt...
Shortsummary MEK 1/2 inhibitor
IC₅₀ & Target 0.4 nM (ERK phosphorylation), 7 nM (MEK1/2)
In Vitro
Cell Viabibibibibibibibiibib lililililililililillitytytytytytytytytytytyty AAAAAAAAAAAssssssssssssssssssssssssssayaaaaaaaaa
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PrPrPrPrPrPrPrPrPrPPrrepepepepepepepepepepepaaaaraaaaaaa ation method The solubility of this compound in DDDDDDDDDDDDDDMSMSMSMSMSMSMSMSMSMSMSMSMSMSMMSOOOOO OOOOOO is >10 mM. General tips for obtaining
a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or
shake it in the ultrasonic bath for a while. Stock solution can be stored below -
20 °C for several months.
Reacting conditions: 0 ~ 1 μM; 72 hrs
Product Name: AZD8330Revision Date: 0 / /20200
etProduct Data Shee
Solvent &&& SSSSSSSoooooolllluuuuuuubbbbbbbiiiiiilllllliiity
Biological Activity
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Applications: AZD8330 decreased viability of MOS and U2OS cells, and significantly
affected 143b cells. In contrast, AZD8330 exhibited no effect on viability of
KPD, ZK58 and Saos-2 cells. According to a capase3/7 activity assay,
AZD8330 at the concentration of 0.5μM induced apoptosis in MOS and U2OS
cells, but not in KPD and ZK58 cells.
In VivoAnimal experiment
Applications:
1. White SM, Avantaggiati ML, et al. "YAP/TAZ Inhibition Induces Metabolic and Signaling Rewiring Resulting in Targetable
Vulnerabilities in NF2-Deficient Tumor Cells." Dev Cell. 2019 May 6;49(3):425-443.e9.PMID:31063758
See more customer validations on www.apexbt.com.
[1]. Zuzanna Baranski, Tijmen H. Booij, Marieke L. Kuijjer, Yvonne de Jong, Anne-Marie Cleton-Jansen, Leo S. Price, Bob van de
Water, Judith V. M. G. Bovée, Pancras C.W. Hogendoorn, Erik H.J. Danen. MEK inhibition induces apoptosis in osteosarcoma cells
with constitutive ERK1/2 phosphorylation. Genes Cancer. 2015 Nov; 6(11-12): 503–512.
FOR RESEARCH PURPOSES ONLY.NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.Specific storage and handling information for each product is indicated on the product datasheet. Most APExBIO products are stable
under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage
temperature. Shortterm storage of many products are stable in the short-term at temperatures that differ from that required for
long-term storage. We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt
of the product, follow the storage recommendations on the product data sheet.
APExBIO Technologywww.apexbt.com
7505 Fannin street, Suite 410, Houston, TX 77054. Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: [email protected]
Product Citations
References
Caution
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Applications: AZD8330 decreased viability of MOS and U2OS cells, and significantly
affected 143b cells. In contrast, AZD8330 exhibited no effect on viability of
KPD, ZK58 and Saos-2 cells. According to a capase3/7 activity assay,
AZD8330 at the concentration of 0.5μM induced apoptosis in MOS and U2OS
cells, but not in KPD and ZK58 cells.
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1. White SM, Avantaggiati ML, et al. "YAP/TAZ Inhibition Induces Metabolic and Signaling Rewiring Resulting in Targetable
Vulnerabilities in NF2-Deficient Tumor Cells." Dev Cell. 2019 May 6;49(3):425-443.e9.PMID:31063758
See more customer validations on www.apexbt.com.
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with constitutive ERK1/2 phosphorylation. Genes Cancer. 2015 Nov; 6(11-12): 503–512.
FOR RESEARCH PURPOSES ONLY.NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTTIC USE.Specific storage and handling informmmmmmmmatatatatatatatatatatataa ioioioioioioioioion for each product is indicatted on the product datasheet. Most AAAAAAAAAAAAAPEPEPEPEPEPEPEPEPPEEPPEExBxBxBxBxBxBxBxBxBBBBEEEEEE IOIOIOIOIOIOOIOOIOIOIOOOIOOO ble prpp oducts are stab
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APExBIO Teechnologywww.apexbt.comxbt com
7505 Fannin street, Suite 410, Houston, TX 77054.Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: [email protected]
Product Citations
References
Caution
Recommended
