SSB -oligo

20-ntoligo

50-ntoligo

21 3 4 5 6 7 8 109 11 12 13

Excess cold 50-nt oligo

5 10 20

50

No

SS

B0 10

0

Excess cold 20-nt oligo

10 20 50

No

SS

B0 10

0

SSB-DNA

Free DNA

SSB (µM): 0 1.3 3.1

9.4

15 nt 20 nt 30 nt 40 nt 50 nt

0 1.3 3.1

9.4

0 1.3 3.1

9.4

0 1.3 3.1

9.4

0 1.3 3.1

9.4

1 2 3 74 5 6 8 9 10 11 21 31 41 51 61 8171 0291

SSB-DNA

Free DNA

SSB (µM):

1 2 3 74 5 6 8 9

(A)50

0 1.3 3.1

9.4

(T)50 (C)50 (G)50

0 1.3 3.1

9.4

0 1.3 3.1

9.4

0 1.3 3.1

9.4

6101 11 21 31 41 51

SSB (µM):

Oligo 1

SSB-ssDNA

ssDNA

0 1.3 3.1 9.4

Oligo 2 Oligo 3

1 2 3 74 5 6 8 9 10 11 12 13

0 1.3 3.1 9.4

0 1.3 3.1 9.4

A B

C D