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Supplemental Figure 2

RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 M; Am: amprenavir; At: atazanavir; D: darunavir; I: indinavir; T: tipranavir) and intracellular proteasome activity was assessed using the affinity probe MV151. The bargraphs represent relative changes in proteasome subunit-specific (1/5 vs. 2) fluorescence signals compared to untreated controls.

RPMI8226 MV-151

β- actin

[µM]

β1(i)/β5(i)

β2β2i

[kD]30

β2/2iβ5/5i/1/1i

Cha

nge

in p

rote

asom

e ac

tivity

]

NLVS 0 10 20 40 0 10 20 40 10 20 40 NLVS 0 10 20 40 0 10 20 40

Am At D I T

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