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Page 1: Supplementary Figure 5 Hori et al

Supplementary Figure 5Hori et al.

Dissected Brain Storage and Sectioning Protocol (Illustrated)

Dissected out Brain Regions

Liquid Nitrogen　

Brain immersed in O.C.T. and 0.1 M PB (containing 20% sucrose) solution

2-Methylbutane (Wako Pure Chemical

Industries Ltd., Osaka)　

To make PB: (68.99 g of NaH2PO4.H20, Merck, was dissolved in final volume of 5 L

Gelatin coated slides made in-house(MATUNAMI MICROSLIDE GLASS

SUPERFROST S2442)

Sectioning

OCT block stored at -80ºC

Sectioning performed at -30ºC

Mounting

Recommended

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SUPPLEMENTARY MATERIAL: SUPPLEMENTARY FIGURE 2cambridge.org:id:article:S... · SUPPLEMENTARY MATERIAL: SUPPLEMENTARY FIGURE 2 Image gallery to accompany John Robb (2015), “Prehistoric
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