Zoology Department , Govt.College University Lahore

Course Title: Animal Cell & Tissue Culture

Course Code: Z-7105

Advances In Animal Cell Culture

Techniques After 2000

Presented By : Sarwar A.D

Keril et al.,

2000

They established a robust, cell-based system for

genetic and functional analyses of HCV

replication in cell culture.

Kwon et al.,

2000

They developed the techniques of Rapid cell

sheet detachment from Poly (N-

isopropylacrylamide)-grafted porous cell

culture membranes (PIPAAm)

Benjamin et

al., 2000

They described the derivation of pluripotent

embryonic stem (ES) cells from human

blastocysts for somatic differentiation in viro.

Major Research In Animal Cell Culture in 2000

Edna et al.,

2001

characterized the composition and function of

adhesions in three-dimensional (3D) matrices

derived from tissues or cell culture.

Nicole et al.,

2001

Enhancement of Hepatitis C Virus RNA

Replication by Cell Culture-Adaptive

Mutations

Annie Sittler et

al.,2001

This is the first demonstration that huntingtin

protein aggregation in cells can be suppressed

by chemical compounds activating a specific

heat shock response.

Chu &

Robinson.,

2001

Industrial choices for protein production by large-

scale cell culture using standard stirred tank

reactors

Potter &

DeMarse.,

2001

They developed a new method for culturing cells

that maintains their health and sterility for many

months for studies

Keefer et al.,

2001

Production of Cloned Goats after Nuclear

Transfer Using Adult Somatic Cells- These results

demonstrated that adult caprine somatic cells

could direct normal development after Nuclear

Transfer.

Shimizu et

al., 2002

Fabrication of Pulsatile Cardiac Tissue Grafts

Using a Novel 3-Dimensional Cell Sheet

Manipulation Technique and Temperature-

Responsive Cell Culture Surfaces

Yang et al.,

2002

Fabrication and surface modification of

macroporous poly(L-lactic acid) and poly(L-lactic-

co-glycolic acid) (70/30) cell scaffolds for human

skin fibroblast cell culture

Shao et al.,

2002

They describe a method, termed SILAC, for stable

isotope labeling by amino acids in cell culture, for

the in vivo incorporation of specific amino acids

into all mammalian proteins

Brinster.,

2002

The recently developed testis cell

transplantation method provides a powerful

approach to studying the biology of the male

germline stem cell and its microenvironment,

the stem cell niche.

Bhadriraju

& Chen.,

2002

They described a review about engineering

cellular microenvironments to improve cell-

based drug testing

Shinohar

a et al.,

2003

Long-Term Proliferation in Culture and Germline Transmission of

Mouse Male Germline Stem Cells- Long-term spermatogonial stem

cell culture will be useful for studying spermatogenesis mechanism

and has important implications for developing new technology in

transgenesis or medicine.

Park &

Shuler.,

2003

Integration of Cell Culture and Microfabrication Technology-

Recent progress in cell culture and microfabrication technologies

has contributed to the development of cell-based biosensors for the

functional characterization and detection of drugs, pathogens,

toxicants, and odorants.

Uemuraa

et al.,

2003

Transplantation of cultured bone cells using combinations of

scaffolds and culture techniques-will a fine step toward bone

regeration therapies.

Beltrami et

al., 2003

They established that Adult Cardiac Stem Cells Are

Multipotent and Support Myocardial Regeneration

Thomas et

al., 2003

Homologous recombination in human embryonic stem cells-

these facts has revolutionized the study of gene function in

mammals, Although most often used to generate knockout

mice, homologous recombination has also been applied in

mouse ES cells allowed to differentiate in vitro. Homologous

recombination is an essential technique if human ES cells are

to fulfill their promise as a basic research tool.

De Ugarte

et al., 2003

They presented the comparison of Multi-Lineage Cells from

Human Adipose Tissue and Bone Marrow- May find their

way in tissue-engineering applications and as gene delivery

vehicles.

Pittenger.,

et al ., 2004

Mesenchymal Stem Cells and Their Potential as

Cardiac Therapeutics, hMSCs isolated from adult bone

marrow provide an excellent model for development of

stem cell therapeutics, and their potential use in the

cardiovascular system is currently under investigation

in the laboratory and clinical settings.

Kohji et al.,

2004

Functional bioengineered corneal epithelial sheet grafts

from corneal stem cells expanded ex vivo on a

temperature-responsive cell culture surface- they have

developed a novel cell-sheet manipulation technology

using temperature-responsive culture surfaces to

generate functional, cultivated corneal epithelial cell

sheet grafts.

Sin et al.,

2004

The Design and Fabrication of Three-Chamber

Microscale Cell Culture Analog Devices with

Integrated Dissolved Oxygen Sensors- provides an in

vitro supplement to animal studies and the possibility

of a human surrogate for predicting human response

in clinical trials.

Kunz-

Schughart

et al.,

2004

The Use of 3-D Cultures for High-Throughput

Screening: The Multicellular Spheroid Model- detect

specific cellular effects reflecting action at various

targets.

Cowan et

al., 2004

Adipose-derived adult stromal cells heal critical-size

mouse calvarial defects- heal critical-size skeletal

defects without genetic manipulation or the addition

of exogenous growth factors.

Fok &

Zandstra.,

2005

Shear-Controlled Single-Step Mouse Embryonic Stem

Cell Expansion and Embryoid Body–Based

Differentiation

These cell-culture tools should prove useful for both the

production of ESCs and ESC-derived cells and for

investigations into adhesion, survival, and differentiation

phenomena during ESC propagation and differentiation.

Jonathan

et al., 2005

Computer-Controlled Microcirculatory Support System

for Endothelial Cell Culture and Shearing was

developed

Fulcher et

al., 2005

Well-Differentiated Human Airway Epithelial Cell

Cultures

Barbulovic

-Nad et al.,

2010

A microfluidic platform for complete mammalian cell

culture- The automated cell microculture technique

Wang et

al., 2005

The Bioreactor: A Powerful Tool for Large-Scale Culture of

Animal Cells

Yaakov et

al., 2005

Laser-guided direct writing for three-dimensional tissue

engineering- underlying processes such as liver and

pancreas morphogenesis, differentiation, and angiogenesis.

Xu et al.,

2005

Inkjet printing of viable mammalian cells

The computer-aided inkjet printing of viable mammalian

cells holds potential for creating living tissue analogs, and

may eventually lead to the construction of engineered

human organs.

Franken et

al., 2006

Clonogenic assay of cells in vitro-colony formation

assay is an in vitro cell survival assay based on the

ability of a single cell to grow into a colony.

Shah et al.,

2006

NucleoCounter—An efficient technique for the

determination of cell number and viability in animal

cell culture processes

Alaminos et

al., 2006

Construction of a Complete Rabbit Cornea Substitute

Using a Fibrin-Agarose Scaffold

Klimanskay

a et al.,

2006,

Human embryonic stem cell lines derived from single

blastomeres

Pampalon

i et al.,

2007

They described that the third dimension bridges

the gap between cell culture and live tissue- they

believe that 3D cultures will have a strong impact

on drug screening and will also decrease the use of

laboratory animals, for example, in the context of

toxicity assays.

Parker &

Townley.,

2007

Biomimetics of photonic nanostructures in cell

culture.

the nanoengineering efficiency of living cells is

harnessed and natural organisms such as diatoms

and viruses are used to make nanostructures that

could have commercial applications.

Chatterje

ea et al.,

2008

Upconversion fluorescence imaging of cells and small

animals using lanthanide doped nanocrystals

this represents the first demonstration of use of

upconversion fluorophores for cellular and tissue

imaging.

Shinohara

et al., 2008

Long-Term Culture of Male Germline Stem Cells

From Hamster Testes.

This technique can be extended to other animal species

in which current genetic modification techniques are

impossible or inefficient.

Paguirigan

& Beebe.,

2008

Microfluidics meet cell biology: bridging the

gap by validation and application of microscale

techniques for cell biological assays

Li et al., 2008 Germline Competent Embryonic Stem Cells

Derived from Rat Blastocysts

Establishment of authentic rat ES cells will

make possible sophisticated genetic

manipulation to create models for the study of

human diseases.

Davis et al .,

2009

Validation of the Cardiosphere Method to Culture Cardiac

Progenitor Cells from Myocardial Tissue

Castell &

Gómez-

Lechón.,

2009

Liver cell culture techniques.

Mesimäki et

al., 2009

Novel maxillary reconstruction with ectopic bone formation

by GMP (good manufacturing practice) adipose stem cells

This is the first clinical case where ectopic bone was

produced using auto ASCs (adipose stem cells) in

microvascular reconstruction surgery and it will pave way for

new clinical trials in the field.

Hiramia et

al., 2009

Generation of retinal cells from mouse and human induced

pluripotent stem cells

Which have a possibility to be used as patient-specific donor cells for transplantation therapies?

Zhao et al.,

2009

iPS cells produce viable mice through tetraploid

complementation.

They reported the generation of several iPS cell lines

that are capable of generating viable, fertile live-born

progeny by tetraploid complementation.

Friedrich et

al., 2009

Spheroid-based drug screen: considerations and

practical approach.

Monitoring of spheroid growth kinetics to determine

growth delay and re-growth, after drug treatment

requires long-term culturing

Fernandes

et al., 2009

Successful scale-up of human embryonic stem cell

production in a stirred microcarrier culture system

Haycock .,

2010

3D Cell Culture: A Review of Current Approaches

and Techniques

Younga &

Beebe., 2010

Fundamentals of microfluidic cell culture in

controlled microenvironments

Uygun et al.,

2010

Organ reengineering through development of a

transplantable recellularized liver graft using

decellularized liver matrix

Sasagawa et

al., 2010

Design of prevascularized three-dimensional cell-

dense tissues using a cell sheet stacking

manipulation technology

Huh et al.,

2011

From 3D cell culture to organs-on-chips-

These ‘organs-on-chips’ permit the study of

human physiology in an organ-specific context,

enable development of novel in vitro disease

models, and could potentially serve as

replacements for animals used in drug

development and toxin testing.

Tekkatte et

al., 2011

“Humanized” Stem Cell Culture Techniques:

The Animal Serum Controversy

Louch et al.,

2011

Methods in cardiomyocyte isolation, culture, and

gene transfer-

Gene transfer techniques for neonatal and adult

cardiomyocytes are also studied, including methods

for transfection (liposome, electroporation) and

viral-based gene delivery.

Centeno et

al., 2011

Safety and Complications Reporting Update on the

Re-Implantation of Culture-Expanded

Mesenchymal Stem Cells Using Autologous

Platelet Lysate Technique

Jesus &

Wurm., 2011

Manufacturing recombinant proteins in kg-ton

quantities using animal cells in bioreactors

Atala., 2011 Tissue engineering of human bladder- A review

AlGhamdi et

al., 2012

Low-level laser therapy: a useful technique for

enhancing the proliferation of various cultured cells

Greiner et al.,

2012

Micro-Engineered 3D Scaffolds for Cell Culture

Studies

Here fabrication techniques, materials, architectures,

biochemical functionalizations, and mechanical

properties of 3D scaffolds were discussed.

Booth et al.,

2012

Acellular Normal and Fibrotic Human Lung Matrices

as a Culture System for In Vitro Investigation

Czekanska et

al., 2012

In Search Of An Osteoblast Cell Model For In Vitro

Research-A review

Castro et al.,

2013

High-throughput SNP-based authentication of

human cell lines (a Solution for cell line mis-

identifications)

Kawamura et

al., 2013

Enhanced Survival of Transplanted Human Induced

Pluripotent Stem Cell–Derived Cardiomyocytes by

the Combination of Cell Sheets with the Pedicled

Omental Flap Technique in a Porcine Heart

Page et al.,

2013

Three-dimensional tissue cultures: current trends

and beyond –A Review

This short review discusses various cell culture

approaches to improve the simulation of the 3D

environment of cells.

Dayeh et al.,

2013

The Use of Fish-Derived Cell Lines for

Investigation of Environmental Contaminants.

Kalos &

June ., 2013

Adoptive T-Cell Transfer for Cancer

Immunotherapy in the Era of Synthetic Biology

Kanatsu-

Shinohara

&Takashi .,

2013

Spermatogonial Stem Cell Self-Renewal and

Development

It will enable us to use these cells for applications in

animal transgenesis and medicine.

Li et al., 2013 Photostable fluorescent organic dots with

aggregation-induced emission (AIE dots) for non-

invasive long-term cell tracing

Landauer., 2014 Designing media for animal cell culture: CHO

cells, the industrial standard.

Kremkow &

Lee., 2015

Sequencing technologies for animal cell

culture research.

Takahashi et

al., 2015

Gene Transfer into Cultured Mammalian

Embryos by Electroporation

Human Genome Projects

Rich media development

Genetic manipulation of cell lines

Development of increasingly sophisticated incubators

Vaccine design

Development of new adhesion surfaces

3D scaffolds

Biotherapeutics

Tissue engineering (for example, the production of tissue in vitro as

skin or cartilage for treatment of burns, auto-grafting, differentiation and induced differentiation (Naderi et al., 2011).

Three-dimensional (3D) cell culture models

(Smalley et al., 2008)

Biorobotics (Zorlutuna et al., 2013).

Cell line misidentifications

Protein expression technologies

New clone screening technologies.

Artificial Chromosome Expression (ACE) System

There is growing interest in controlling matrix stiffness, a concept that has led to discoveries in fields such as:

Stem cell self-renewal

Lineage specification

Cancer cell phenotype

Fibrosis

Hepatocyte function

Mechano-sensing

The in vitro micronucleus technique (Michael.,2001)

Dynamic macroscale bioreactor systems