BIOE201MICROBIOLOGY PRACTICAL

COURSE

By Huzaifa Umar

Personal Safety

Safety Precautions that Must be Adhered to.

Laboratory Safety Equipment

Measures to Avoid During Microbiology Practical

Presentation of Results

Microscope

Uses of Microscope

Anatomy of Microscope

Outlines'

Prevention of infection that might be caused by the

microorganisms being studied.

The reagents, equipments and procedures used are

potentially hazardous.

Attention to proper procedures and prudent laboratory

practices are required for your safety and protection.

Personal Safety

Wash your hands with disinfectant soap.

Always wear a clean laboratory coat.

Food, drinks, chewing gum, or smoking is

allowed in the laboratory.

Disinfect work areas before and after use

with 70% ethanol or fresh 10% bleach.

Do not place materials and apparatus in

your mouth (Including plugged pippetes.

Safety Precautions

Discard contaminants, cultures and

apparatus biohazard container/bags

provided and autoclave it before

discarding.

At the end of the laboratory period,

benches must be cleared and swabbed

with disinfectants.

On leaving the lab, hands must be wash

thoroughly and lab coat must be removed.

Cont...

Biohazard bag

Biohazard Container

Laboratory Equipments

Biosafety Cabinet (BSC) Eye Wash

Fire ExtinguisherFirst Aid Kit

Take all necessary measures to avoid contamination of microbial

growth media, tubes or plates.

Identify the containers of the inoculated media

Place the media for incubation in identifies receptacles.

In presentation of results; + sing represents growth and – sign

represents no growth.

All results must be followed by well articulated reports.

Things you should know while carrying out microbiology practical

Microscope

Eyepiece: The lens the viewer looks through to see the specimen.

The eyepiece usually contains a 10X or 15X power lens.

Head: The body tube connects the eyepiece to the objective lenses.

Arm: The arm connects the body tube to the base of the

microscope.

Revolving noisepiece (Revolver): Use to change the magnifying

lenses.

Objective Lens: Lens closest to the object and it has magnifying

lenses, usually 4x, 10x, 40x and 100x.

Function of a Microscope

Specimen or slide: The specimen is the object being

examined. Most specimens are mounted on slides, flat

rectangles of thin glass.

Illumination: The light source for a microscope.

Condenser: Gathers the light rays from light source and

focuses them onto specimen.

Coarse and fine adjustment knobs: used to focus the

specimen.

Cont….