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Mitochondria (singular, mitochondrion) – are typically tubular or rod-shaped
organelles found in the cytoplasm of most cells and produces enzymes for the metabolic conversion of food to
energy.
Energy conversion
The most prominent roles of mitochondria are to produce the energy currency of the cell, ATP, through respiration, and to regulate cellular metabolism.
A dominant role for the mitochondria is the production of ATP, as reflected by the large number of proteins in the inner membrane for this task.
History
Mitochondria was first observed by kollikar in 1850
The present name was given by Benda in 1997
In 1882 flaming named the mitocondria flia
In 1912Kingsburry was suggested that oxidation raection are carried in mitocondria
Wotto warberg is father of respiratory he isolated large mitocondria molecule and found the enzyme associated withOxidaton process
STRUCTUREA mitochondrion contains outer and inner
membranes composed of phospholipid bilayers and proteins. The two membranes have different properties. Because of this double-membrane organization, there are five distinct parts to a mitochondrion. They are:
- encloses the entire organelle, has a protein-to-phospholipid ratio similar to that of the eukaryotic plasma membrane
Outer Membrane
contains large numbers of integral
proteins called porins. allow molecules to freely
diffuse from one side of the membrane to the other.
Outer membrane
It is also known as Perimitochondrial space.
Because the outer membrane is freely permeable to small molecules, the concentrations of small molecules such as ions and sugars in the intermembrane space is the same as the cytosol.
Intermembrane space
FUNCTIONS:
those perform the redox reactions of oxidative
phosphorylation
ATP-synthase
Specific transport proteins
Protein import machinery
Mitochondria fission and fusion protein
Inner membrane
CRISTAE
- The folding of the inner membrane that allows more surface area,
enhancing its ability to produce ATP.
The matrix is the space enclosed by the inner
membrane.
It contains about 2/3 of the total protein in a mitochondrion.
The matrix is important in the production of ATP with the aid of the ATP synthase contained in the inner membrane.
Matrix
Chemical composition:
The outer membrane consists of 40% lipids and 60 percent proteins.
The inner membrane is made up of 20% lipids and 80% proteins. The electron transport enzymes, proton secreting proteins are virtually buried in the core of the inner membranes.
Mitochondrial matrix also consists of a wide variety of enzymes. There are more than 120 kinds of enzymes of mitochondrial.
Mitochondrial matrix also contains DNA, RNA molecules.
Proteins encoded by mitochondrial - synthesized
on ribosomes within the organelles and directed to sub-compartment immediately
Most proteins within mitochondria – coded by nuclear DNA, imported through ER
Sorting of Proteins to Mitochondria
Localize proteins to mitochondria
20–50 amino acids in long, located at the N-terminus
Rich in hydrophobic amino acids, +ve charged basic amino acids (arginine and lysine), and hydroxylated amino acids (serine and threonine)
Generally lack -ve charged acidic residues (aspartate and glutamate)
Amphipathic - Assume an α-helical conformation with +ve amino acids on one side of the helix and hydrophobic amino acids on the other side
Mt have ~1000 general import pores
Only unfolded proteins can be imported into the mitochondrion
Chaperone proteins (Hsc70) keep nascent and newly made proteins in anunfolded state
Mitochondrial matrix-targeting sequences
Tom proteins for translocon of the outer membrane
Tom20, Tom22 – recognize N-terminal matrix targeting sequences
Tom40 – constitutes outer general import pore (unidirectional passive channel)
Tim23, Tim17 – Compose the inner membrane channel (Tim - translocon of the inner membrane)
Outer-membrane peptide import receptor proteins on mt
Precursor proteins are kept unfolded by
chaperones (Eg. Hsc70) Hsc70 - interacting with Tim44 and
localized to the translocation channels in the inner mitochondrial membrane
Precursor protein binds to an import receptor - near asite of contact with the inner membrane
Precursor will be transferred into the general import pore
Protein import into the mitochondrial matrix
(i) ATP hydrolysis by Hsc70 chaperone proteins This energy is spent to maintain bound precursor proteins in an
unfolded state
(ii) Matrix Hsc70 + Tim44 protein - molecular motor to pull the protein into the matrix
(iii) H+ electrochemical gradient (Protonmotive force) across the inner membrane
Transmembrane potential - 200 mV (4,00,000 V/cm electric gradient) Positive charges in the amphipathic matrix-targeting sequence is
electrophoresed into the matrix space by the inside-negative membrane potential
Three energy inputs for the import of proteins to Mt
Path B
Precursors contain both matrix-targeting sequence and internal multiple hydrophobic domains recognized by inner-membrane protein termed Oxa1
Translocate a portion of the precursor into matrix through Tom20/22 and Tim23/17 channels
Matrix-targeting sequence gets cleaved, protein inserted into the inner membrane (by interaction of Oxa1 and other inner-membrane proteins)
Path C For multi-pass proteins with 6 helices Precursor lacks N-terminal matrix-
targeting sequence but has multiple internal mitochondrial targeting sequences
Internal sequences on precursor are recognized by Tom70 (a second import receptor in the outer membrane)
Protein passes through Tom40 Second translocation complex in the
inner membrane is composed of Tim22/54
Tim9 and Tim10 (small proteins in inter-membrane spaces) transfer precursor to Tim22/54
Tim22/54 incorporate the multiple hydrophobic segments to the inner membrane
(ii) Targeting of Mt inter-membrane space proteins
Path A Precursor carries two different N-
terminal targeting sequences (both will be cleaved)
Targeting sequence closer to n-terminal is removed by Protease
The second targeting sequence (a hydrophobic segment) blocks complete translocation of the protein across the inner membrane
Protein gets inner-membrane embedded and diffuses laterally
A protease in the membrane cleaves the protein near the hydrophobic transmembrane segment
Mature protein is released into the intermembrane space
Path B No inner-membrane translocation factor is
involved Precursor is delivered directly to the
intermembrane space through general import pore
(iii) Targeting of Mt outer-membrane proteins A short matrix-targeting sequence at the N-
terminus is followed by a long stretch of hydrophobic amino acids
Hydrophobic sequence - stop-transfer sequence - prevents transfer of the protein into the matrix and anchors it in outer membrane
Neither the matrix-targeting nor stop-transfer sequence is cleaved from the anchored protein