Nanomedicine S.A. Laimos High School

Quantum Spin-Off project is funded by the European Commission. This communication reflects the views of the author, and the Commission cannot be held responsible for any use which may be made of the information contained herein.

Nanomedicine S. A. Laimos Junior and Senior High SchoolAcademic year 2014-2015Participation in the European Nanotechnology Contest for High School students “Quantum Spin-Off” An action belonging to the European Program “Inspiring Science Education.” (I.S.E.)

Presenters: Anastassiadou Andriana Charissi Zoi Delkou Kiriaki Kazoglou Antigoni Kazoglou Evangellia Nalpantidou Christina Sampsonidou- MichaiIidou Georgia Sampsonidou- Michailidou Penelope Sotiri Dimitrios Paraskevopoulou Aristea Tsili Antonia

12 May 2015 Laimos Junior and Senior High School 2

Structure of the presentation 1st Part: the product- magnetic nanoparticles for applications of magnetic hyperthermia.2nd Part : the establishment and the characteristics of the Spin- Off company.3rd Part: the medicine market in Greece.


1st Part The product: magnetic nanoparticles for applications of magnetic hyperthermia


What is nanotechnology?

Nanoscience and nanotechnology are the study and the application of extremely small things that can be used across all the science fields.

Science

Engineering

Technology

conducted at the nanoscale (which is about 1 to 100nm, where 1 nm

= 1: 1.000.000.000 m).


Magnetic Hyperthermia Magnetic hyperthermia is an experimental cancer

treatment which destroys tumors but does not affect the neighboring healthy cells.

Magnetic nanoparticles are put inside a tumor and the whole patient is placed in an alternating magnetic field of well-chosen amplitude and frequency.

The tumor temperature rises until the cancer cells start dying (43 oC).

Our main product is Nanoferrum, a medicine with magnetic nanoparticles, which is applied in the method of magnetic hyperthermia.


A special case: Superparamagnetism • When a ferromagnet or ferrimagnet is sufficiently

small, it acts like a single magnetic moment. • Its response to an external magnetic field is like a

paramagnet, but much stronger. • This is the case of the nanoparticles of magnetite

(Fe3O4) and maghemite (γ-Fe2O3, where γ refers to the crystalline structure), which are applied in the method of magnetic hyperthermia.


Synthesis of Magnetite (Fe3O4) Nanoparticles (1)• In a glass container with 30 mℓ of water we dissolve

under continuous stirring, 150 mg of dextrane, at about 35οC.

• In 10 mℓ of water we dissolve 0.0026 moles of dichloride and trichloride ferric in moles portion 2:1, with simultaneous addition of 30μℓ of concentrated hydrochloric acid(HCl 37%).


Synthesis of Magnetite (Fe3O4) Nanoparticles- (2)• The temperature in the container rises up to 40οC, so 2

mℓ of ammonium hydroxide (NH4OH, 30%) and the solution of ferric ions are added.

• In the first 15 minutes of the reaction the temperature rises further gradually to 55οC and it is stabilized at 60-70οC for 80 min.

• The container is removed from the hob and the product is placed in a water bath to be cooled at 30οC.


Cleaning the final product (1) • Initially the product is shared into 10 vials of 2

mℓ and centrifuged (we use a centrifuge device by Bacacos Scientific).

• After the centrifugation, the supernatant phase was discarded while the precipitate was collected from the flasks and added to half the amount of water (10 mℓ H2O).

• The product is stirred for 15 min (VORTEX).


Cleaning the final product (2) • The product is transferred to another container and then

it is placed in an ultrasonic bath for 15 min in order to reduce the aggregates generated by centrifugation.

• The above process of centrifugation and stirring is repeated.

• A mild centrifugation of the colloid for 20 min at 2100g follows for removing larger aggregates by precipitation.

• Using a pipette, the bulk of the headspace in the vials is isolated and the colloid is stored in the refrigerator (at 7 ° C) to be examined in the electron microscope.


Characterising the final product (1) • We use a Leitz Scanning Electron Microscope (S.E.M.),

model Amr 1600T, which belongs to the Ceramic Materials’ Research Laboratory (University of Athens, Department of Physics, Solid State Physics’ Section).

• Its function is based on the presence of spatially varying magnetic fields, which act as "magnetic lenses".

• The samples are prepared for the S.Ε.Μ. by placing a few droplets of the colloid in appropriate carriers and putting them in an oven at 37οC until they are completely dry.


Characterising the final product (2) • The samples are coated with gold under vacuum.• The samples enter the microscope, where we an

appropriate 20,00 kV acceleration potential is used.• The samples are enlarged by 2,000 and 5,000 times. In

all cases we can see ferric oxide nanoparticles aggregated to each other (thrombosis or flocculation of the colloid emulsion).


2nd Part The Business Model Canvas


The goal of Nanomedicine S.A. Success


Business Model Canvas

Success


Value Preposition

Our products:• Thanks to the nanoparticles they contain,

they target only tumors.• They do not afflict the body with side effects• They are made from natural products, which

with the help of nanotechnology are homogenized and take the form of injectable solutions.

• They promise healing and good quality of life• They are cheaper than the respective ones

already marketed.


Customer Segments

• Private clinics• Public hospitals• Doctors- Prescribers• Pharmacies/pharamceutical depots


Distribution Channels • Medical salesmen• Presence in scientific conferences and

medicine or pharmacology workshops.• Participation in pharmaceutical fairs• Advertisement of our products via

brochures/leaflets etc…• Advertising in medical journals• Creation of a website for online advertising The distribution of products will be accomplished by means of trucks through highways.

Our company’s site: users.sch.gr/ivelonak/joomla2015


Customer Relations

• We are involved in sponsoring hospital and clinic events during festive and commemorative days

• We cover doctors’ medical expenses to attend scientific and medical conferences

• We offer gifts during festive periods


Key Activities • Finding suppliers according to their reliability, pricing policy

and their response to the requirements of product and service improvement

• Acquisition of machinery• Scientific reserch• Medicine Manufacturing• Advertizing• Sale• Payment of all parties involved (human resources, suppliers,

collaborators etc)


Key Resources

• Human Resources of various specialties• Raw materials• Equipment/machinery• Capital (premises, vechicles)• Cash/Money


Key Partners • Suppliers of raw materials• Suppliers of packaging materials• Company’s human resources• Advertisers Everybody’s needs must be met for • Profit • Reputation • Success


Revenue Streams • Company’s revenue source is its products’

sales• Customers can pay via bank cheques and e-

payments• Nanomedicine S.A. has a stable pricing policy.• Takes into account the volume of transactions• Monitors the fluctuations of the raw

materials’ market price and creates its price list accordingly, providing frequent updates to customers.


Cost Structure • Purchase/rental of facilities• Purchase of

electrical equipment / machineryraw materials packaging materials cars/vehicles

To these, we should add the cost of: • Advertising • Human resources • Product distribution• Lighting• Heating• Water supply• Stationery


3rd Part Establishing our company


The Medical Market in Greece (1)• The drug is a social good, so the institutional framework

defines drastically: the production the distribution the storage the quality assurance the competition the pricing the prescription policy the general market conditions


The Medical Market in Greece (2)The production and distribution of pharmaceutical products is one of the most dynamic sectors in Greek industry: increased investments in the sector creation of new units of production that manufacture

pharmaceuticals. It is a dynamic industry which makes high profit.


The establishment of Nanomedicine S.A.We created the Spin-Off company named Nanomedicine S.A. for manufacturing and selling nanomedicines.It is headquartered in the Industrial Area of Florina.The company is going to be set in a 300 sq.m. building that will be rented for this purpose.


Necessary Formalities• Issuing of books and documents• Tax books are confidential and nobody has the right to

take knowledge of them, apart from the tax office• Decide on the necessary documents, which are:

invoicesdelivery notes waybillscheck books block collection receipts.


Nanomedicine’s Chart

CEOIoannis Kapouranis

Deputy CEOAthanassios Trentos

Internal Audit and

Information Technology

D im itrio s So tiriCh ristin a N alp an tidouTh eodo ra Lykom an i

Legal departme

nt

A ristea Paraskevopou louG eo rgia Samp son idou -M ich ailidou

Department of quality assurance

Pen elop e Samp son idou - M ich ailidouAnd rian a An astasiadou

Research and Developmen

t Department

Zo i Ch arissiElias Pap adopou lo sK iriaki D elkouKon stan tino s Stavropou lo s

Public Relations

Evan gelia K azo glouAn ton ia Tsili

Marketing

Human Resources Manager Raphail Nalpantidis

Our company chart includes: CEO and his deputy Human Resources Manager

And the following sections:

Internal Audit and Information Technology Legal department Department of quality assurance Research and Development Department Public Relations officer Marketing officer


Financing the Company• Equity• Funded by the Ministry of Development as a spin-off

company upon request• Check the company's possibilities to be funded by the

Operational Program named: “Competitiveness , Entrepreneurship and Innovation”.


Nanomedicine’s Website and Blog The website creation is crucial for the company’s advertising since suppliers and customers can be informed about our activities. Also, we have created a blog for improving the internal communication of our team.

Our company ‘s site: users.sch.gr/ivelonak/joomla2015

Our blog: http://www.nanophysicslaimos.blogspot.gr

http://www.nanophysicslaimos.blogspot.gr/


Nanomedicine’s objective and vision “Possessing flexibility, speed and adaptation to the new requirements we aim to establish ourselves in a leading pharmaceutical company and offer the community and the Greek economy!! We incorporate new technologies in our work and we rely on our people, who are our strength. Working in Nanomedicine S.A. is aimed to be considered as an important milestone for the realization of their professional ambitions! Together we work with vision, dedication and loyalty to produce quality medicines for the treatment of diseases!”

Read more in our company ‘s site: users.sch.gr/ivelonak/joomla2015


Useful References

Bakandritsos, G. Mattheolabakis, G. Chatzikyriakos, T. Szabo, V. Tzitzios, D. Kouzoudis, S. Couris, and K. Avgoustakis (2011), Doxorubicin Nanocarriers Based on Magnetic Colloids with a Bio-polyelectrolyte Corona and High Non-linear Optical Response: Synthesis, Characterization, and Properties, Adv. Funct. Mater., 21, 1465–1475

Baron A.R. & Shane S.A. (2008) Entrepreneurship. A process perspective. 2nd edition. South Western.

Fred R.D. (2011) Strategic Management. Concepts and cases. 13th edition. Pearson.

Goldstein J, Newbury D, Joy D, Lyman C, Echlin P, Lifshin E, Sawyer L and Michael J (2003). Scanning electron microscopy and x-ray microanalysis. Springer.

Kotler P., Brady M., Goodman M. & Hansen T. (2009) Marketing Management. Pearson.

Sutcliffe, H. (2011). A report on responsible research and innovation. Brussels: Matter.

Wanquan Jiang, H.C. Yang, S.Y. Yang, H.E. Horng, J.C. Hung, Y.C. Chen, Chin-Yih Hong (2004), Preparation and properties of superparamagnetic nanoparticles with narrow size distribution and biocompatible, Journal of Magnetism and Magnetic Materials, Volume 283, Issues 2–3, Pages 210-214, ISSN 0304-8853 http://dx.doi.org/10.1016/j.jmmm.2004.05.022.(http://www.sciencedirect.com/science/article/pii/S0304885304006304)

http://dx.doi.org/10.1016/j.jmmm.2004.05.022.(http:/www.sciencedirect.com/science/article/pii/S0304885304006304)

http://dx.doi.org/10.1016/j.jmmm.2004.05.022.(http:/www.sciencedirect.com/science/article/pii/S0304885304006304)


End of presentation

Thank you for your attention!!!

Read more in our blog: http://www.nanophysicslaimos.blogspot.gr

http://www.nanophysicslaimos.blogspot.gr/