______________________________________1. Name 2.Type3.Aim 4.Principal 5.Reagent 6. steps 7.Result 8. conclusion 9. MLI in spectroscope

Blood : 6 tests

3 preliminary : • kastle mayer pink(20 gm Koh __20gm zinc_ 2gm phenolphthalein _100 ml water )

• benzidine _ (benzidine in glacial acetic acid )blue • guciacum (guciacum resin in ethanol ) green Peroxidase enzyme oxidation of reagent in O2 source colour3 confirmatory :• spectroscope (blood absorb light and give bands )_ distilled water1:102 bands (carboxy or oxy ) ___1 band (reduced Hb __( 4bands metHb )_ 2( one thick )hemachromagen )))))DON’T FORGET ( MLI) • Teichmann s __ )hematine )_anhydrous glacial acetic acid_ rhombic

crystal • takayama (haemochromagen ) __ 3v( NAOH _ Pyridine _ glucose )

7v (water ) needle shape crystales

Semen : Florence choline 1.5gm K iodide __2.5gm iodine __30ml water

Rhombic shape small then enlarged disappear choline per iodide

Barberios spermin alcoholic solution of picric acid needle shape crystal

Reinsch test

1. Name : reinsch2. Type : preliminary

3. Aim : diagnosis heavy metals

4. Principal : heavy metals deposit on copper foil in presence of HCL

5. Reagent : 10% HCL+ copper foil 6. steps : contamination _ colour changes __ sublimation _

7. Result :…………………..8. advantages :easy _cheap _quick _ indicate small amount

9. Disadvantages : qualitative not quantitative

Copper foil

Hair and fibers

medulla

Silk

Cotton

wool