Vincent Denef Ecology and Evolutionary Biology, University of Michigan

Water @ Michigan

January 21, 2016

The difference a species makes: impact of invasive species on freshwater microbial

communities

@VJDenef

vdenef@umich.edu

The difference a species makes

Texasinvasives.org

Invasion of the Dreissenids

[Credit: Nalepa, GLERL

Invasion of the Dreissenids The difference a species makes

[Higgins, Ecol Mono, 2010] Bacteria 0 ? ? ?

Invasion of the Dreissenids The difference a species makes

Biota Phytoplankton Micro/nanozooplankton Phototrophic

bacteria

Ecosystem impacts

Chemotrophic Bacteria (benthic-pelagic)

Microbial higher food web connectivity

Geochemical cycling (e.g. CO2 efflux)

[Buffam et al, GCB, 2011]

July 99 July 10

[Credit: Vanderploeg, GLERL]

Chl a concentration

Impacts on bacterial communities

[Credit: Vanderploeg, GLERL]

Harmful algal blooms (e.g., Microcystis)

The difference a species makes

Source water: Lake MI M45, 100 micron pre-filtered Quagga mussels: Lake MI M45, cleaned and acclimated in lab for 12 hrs

20L

3 hrs 4 C

20L

20L

20L

X 3

X 4

X 3

X 4

16S rRNA V4 sequencing

Tom Johengen Hank Vaderploeg

Experimental design Feeding experiments

* = statistically significant difference (LEfSe, p < 0.05)

Impacts on bacterial communities: feeding experiment Feeding experiments

Impacts on bacterial communities: field observations Field observations

Impacts on bacterial communities: field observations Field observations

Acknowledgments

NOAA GLERL Ashley Baldridge Joann Cavaletto Jim Liebig Ed Rutherford Henry Vanderploeg Crew of the R/V Laurentian

University of Michigan Michelle Berry Ashley Burtner Edna Chiang * Seana Florida * Masanori Fujimoto *** Thomas Johengen Ann McCarthy Ryan Keeling * Morgan Meade * Justin Millar Tom Nalepa Danna Palladino Marian Schmidt ** Amelia Waters * Amadeus Twu *

Support

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