Figure (1): NO production by Dextran coated vs. Lipid coatedSPIO particles in Macrophages-24 h

SPIO-4SPIO-2 SPIO-3 Control0.0

0.1

0.2

0.3

SPIO-4

SPIO-2SPIO-3

Control

OD

550

(Nitr

ite le

vel)

10.0 5.0 2.5 1.3 0.6 0.00

1

2

3

4

ug/mL FITC SPIO

AFU

/wel

l S

D

Figure (3) A: macrophages harvested from mice injected with FITC-SPIO show intracellular fluorescence. The same panel stained with DAPI to show nuclear stain and viability. B: Circulating monocyte, shows iron particles (arrow), after the injection of SPIO.

A B

Dark (negatively enhanced) aortic wall, full of iron particles

Bright aortic lumen and wall without negative enhancement and no significant number of iron particles in pathology

Before Injection After Injection (5 Days )

Apo E Deficient mouse

C57B1 (control) mouse

Figure 7: