Honolulu Cloning TechniqueBy: Annie Reich

Block: 4

What is the Honolulu Technique?

• Cloning: when multiple identical copies of a DNA sequence are made. Cloning is also used to create an organism from a clone cell.

• Honolulu Technique: o one of three ways of cloning o produce live, healthy, cloned male or female offspring

from the fibroblast cells of adult animals

Facts

• Developed by Teruhiko Wakayama and Ryuzo Yanagimachi at the University of Hawaii.

• Scientists have successfully produced five generations of healthy cloned mice.

• Scientist believe that in the future Honolulu Technique can be used to treat AIDS, cancer, multiple sclerosis, and also diabetes.

• 3 out of every 100 attempts are successful.

Steps of Honolulu Technique

• Step 1:– A cell’s nucleus is removed from the organism being

cloned.

• Step 2:– Next a donor nucleus is transferred into an egg.

• Step 3:– Then the egg is treated with special chemicals and taken

care of until it is able to be placed into the surrogate mother.

• Step 4:– An identical cloned baby is made.

• Step 5:– Process is repeated over and over again in order to make more copies.

Pros• Creates exact copies

• more successful than the Roslin Technique

• Scientist believe this technique can be used to treat AIDS, cancer, multiple sclerosis, and also diabetes.

• Scientist can test this technique to broaden their understandings and learn more useful ideas on ways to use the technique

Cons• Expensive

• Reduces genetic diversity

• Created many mutations before it was successful

• If a virus gets into the mix it can knock out a whole generation of clones

Bibliographyhttp://biology.about.com/od/biotechnologycloning/ss/cloning-clinic_2.htm

http://library.thinkquest.org/24355/data/details/techniques/honolulu.html

http://www.humancloning.org/threeways.htm

http://robby.nstemp.com/shopping_page.html

http://library.thinkquest.org/24355/data/details/media/honoluluanim.html