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FAMILY CALICIVIRID
AEKasahun Delenee VAR/5994/05
CALICIVIRUS• from the Latin calyx “chalice” or
Cup• Often referred as “Small round
structured viruses”
VIRION AND NUCLEIC ACID PROPERTIES
• non-enveloped• 27-40 nm in diameter• Icosahedral symmerty• Some have characteristic appearance with
32 cup shaped depressions on their surface
• Linear• Positive sense single-stranded RNA viruses
VIRION AND NUCLEIC ACID PROPERTIES
VIRION AND NUCLEIC ACID
PARTICLESEncompasses 4 clinical syndromes:• Swine Vesicular Exanthema• San Miguel Sea Lion Virus• Feline Calicivirus• Rabbit Hemorrhagic Disease
TAXONOMYGroup IV; single stranded RNA positive-strand virusesFamily: CaliciviridaeGenus: Lagovirus
Nebovirus Norovirus Sapovirus Vesivirus
REPLICATION1.Attachement to ho
st receptors mediates endocytosis of the virus into the host cell.
2.Uncoating, and release of the viral genomic RNA into the cytoplasm.
3.VPg is removed from the viral RNA, which is then translated into a processed ORF1polyprotein to yield the replication proteins.
4.Replication occurs in viral factories. A dsRNA genome is synthesized from the genomic ssRNA(+).
REPLICATION
REPLICATION5. The dsRNA genome
is transcribed/replicated thereby providing viral mRNAs/new ssRNA(+) genomes.
6. Subgenomic RNA translation gives rise to the capsid protein and VP2.
REPLICATION7. Assembly of new
virus particles and release by cell lysis.
DISEASES• Swine Vesicular Exanthema• San Miguel Sea Lion Virus• Feline Calicivirus• Rabbit Hemorrhagic Disease
Vesicular Exanthema infection of Porcine
DISEASES
Rabbit Hemorrhagic Disease
DISEASES
FELINE CALICIVIRUS
(FCV)
FELINE CALICIVIRUS(FCV)
a common respiratory disease in cats virus attacks the respiratory tract -- lungs and
nasal passages -- the mouth, with ulceration of the tongue, the intestines, and the musculoskeletal system
FELINE CALICIVIRUS (FCV)
Causative agent: Vesivirus
Clinical Signs: conjunctivitis rhinitistracheatis pneumona and
vesiculation,ulceration of the oral epithelium fever anorexia lethargy stiff gait
Nasal discharge and vesicles & ulcers on the tongue of a cat infected with FCV.
FELINE CALICIVIRUS (FCV)
Tongue ulcerative glossitis from a FCV case.
FELINE CALICIVIRUS (FCV)
Lesions: on muzzle and tongue, respiratory tract, oral cavity and eyes.
Pathogenesis: natural transmission occurs via aerosol and fomites; the virus is often carried to susceptible cats by human handlers.
FELINE CALICIVIRUS (FCV)
Radiographs of interstitial pneumonia.
FELINE CALICIVIRUS (FCV)
Histopathology & gross pathology of interstitial pneumonia from a FCV case.
FELINE CALICIVIRUS (FCV)
Lymphocytic-plasmacytic stomatitis in a cat.
FELINE CALICIVIRUS (FCV)
Lesions in the eyes
FELINE CALICIVIRUS (FCV)
SAMPLES AND TESTSDISEASES
CAUSATIVE
AGENT
SAMPLE/s DIAGNOSTIC TEST
SWINE VESICULA
R EXANTHEM
A
VESIVIRUS Vesicular fluid, mucous membranes
SEROLOGIC TESTELECTRON MICROSCOPY
FELINE CALICIVIR
US
VESIVIRUS oral swabs, blood, skin scrapings or lung
tissue
PCR, IMMUNOFLOURESCENCEENZYME IMMUNOASSAY
RABBIT HEMORRH
AGIC DISEASE
LAGOVIRUS Spleen, liver , blood IMMUNOFLOURESCENCEENZYME IMMUNOASSAY
SAN MIGUEL
SEA LION VIRUS
INFECTION
VESIVIRUS Tissue samples, fecal samples
ELISAImmunoflourescencePCR
CONTROL AND TREATMENT
FELINE CALICIVIRUS There is no specific treatment for FCV Antibiotics are used for secondary bacterial
infections, and immune modulators, such as lymphocyte T-cell immune modulator, have been used for immune support.
Nursing care and rehydration are used for dehydrated and anorexic cats
Routine vaccination.
Avoiding overcrowding of feline populations, reducing stress.
THE END